The main aim of the present study was to compare skeletal maturity level and physical capacities between male Norwegian soccer players playing at elite, sub-elite and non-elite level. Secondary, we aimed to investigate the association between skeletal maturity level and physical capacities. One hundred and two U14 soccer players (12.8-14.5years old) recruited from four local clubs, and a regional team were tested for bone age and physical capacities. Bone age was estimated with x-ray of their left hand and used to indicate maturation of the skeleton. Players went through a comprehensive test battery to assess their physical capacities. Between-groups analysis revealed no difference in chronological age, skeletal maturity level, leg strength, body weight, or stature. However, elite players were superior to sub-elite and non-elite players on important functional characteristics as intermittent-endurance capacity (running distance: 1664m±367 vs 1197m±338 vs 693m±235) and running speed (fastest 10m split time: 1.27seconds±0.06 vs 1.33seconds±0.10 vs 1.39seconds±0.11), in addition to maximal oxygen uptake ( ), standing long jump, and upper body strength (P<.05 for all comparisons). Medium-to-large correlations were found between skeletal maturity level and peak force (r=695, P<.01), power (r=684, P<.01), sprint (r=-.471, P<.001), and jump performance (r=.359, P<.01), but no correlation with upper body strength, , or intermittent-endurance capacity. These findings imply that skeletal maturity level does not bias the selection of players, although well-developed physical capacity clearly distinguishes competitive levels. The superior physical performance of the highest-ranked players seems related to an appropriate training environment.