Northwestern Iran Research Articles

Effects of banqueting on water infiltration and physico-chemical properties of soil in semi-arid lands

Banqueting is a simple and cheap soil conservation practice in natural rangelands under dry climates. Little research exists about its impacts compared to other land uses, where agriculture and land abandonment may be important reasons for degradation. This study has evaluated water infiltration and key physico-chemical properties of soil in natural rangelands with banquets in West Azerbaijan (North-Western Iran) compared to: (i) undisturbed sites (assumed as reference condition) and (ii) cultivated or abandoned dry farmlands. Under these soil conditions, the texture, bulk density, porosity, water content, pH, electrical conductivity, organic carbon and carbonate contents of soil (using common physical-chemical methods) as well as the hydraulic conductivity (using a single-cylinder infiltrometer) were measured in the topsoil (0–20 cm). This data was further processed by applying the Principal Component Analysis and the Agglomerative Hierarchical Cluster Analysis. In areas with banquets, soil hydraulic conductivity was higher by 84% compared to dry farmlands (abandoned or not), where water infiltration was noticeably reduced (−44%) compared to the reference soil condition. Moreover, a noticeable increase in organic carbon content (+80%) and a lower salinity (−36%) of soil compared to cultivated areas was measured. These effects are important to enhance soil fertility on one side, and resistance to erosion on the other side. Therefore, in the experimental areas, banqueting may counteract the degradation of soil due to the agricultural activity as well as its abandonment, and restore the properties of the undisturbed soils that are typical of natural rangelands. The results of this study may be of help for landscape managers and agronomists for the pressing needs for soil conservation and productivity in semi-arid areas that are prone to degradation and abandonment.

Molecular Identification, Multigenic Characterization and Phylogenetic Analysis of Ctenocephalides Canis Fleas from Humans and Domestic Animals in Iran.

Fleas belonging to the Pulicidae are prevalent ectoparasites infesting mammals and birds in Iran. This study focused on genetically identifying and characterizing Ctenocephalides canis collected both off-host and infesting humans and various domestic animals in the country. A total of 918 adult flea samples were collected from 10 sites in western and northwestern Iran between April 2018 and May 2019. Out of these, 71 specimens were found off-host, while the remaining fleas were collected from humans (121), sheep (126), goats (184), and dogs (416). Morphological identification at the genus level was performed on all fleas, and ten selected specimens selected based on the sampling sites and hosts were subjected to molecular detection at the species level by using partial amplification and sequencing of the internal transcribed spacer 1 and 2, as well as the cytochrome oxidase I (COXI) markers. The morphological identification confirmed all fleas as Ctenocephalides spp. Alignment and phylogenetic analysis of nuclear and mitochondrial partial sequences confirmed the presence of C. canis. However, molecular divergence was observed among the ten isolates based on the ITS1 and ITS2 with diversity rates estimated at 0.15% and 3.36%, respectively. Notably, the analysis of the COXI marker revealed no molecular divergence among the partial sequences representing the ten studied isolates from C. canis. This study explores the diversity of C. canis in the western and northwestern regions of Iran, providing insights into their molecular taxonomy and potential role as disease vectors in these areas.

Seromolecular study on the prevalence and risk factors of Toxoplasma gondii infection in pregnant women referred to a gynecology hospital in Urmia, northwest part of Iran in 2022

Toxoplasmosis is a frequent infection among the human population. The infection can cause devastating complications for the fetus during pregnancy. The present study aimed to determine the serological and molecular prevalence of the infection and molecular characterization of Toxoplasma gondii isolates among pregnant women referred to Kowsar Hospital, Urmia, Iran. In a cross-sectional study, 340 blood samples were collected from pregnant women referred to Kowsar Hospital, Urmia, Iran from May to July 2022. Anti-T. gondii IgG and IgM seropositivity were determined by enzyme-linked immunosorbent assay. PCR was carried out by targeting the GRA6 gene of the parasite on all patients’ buffy coats. Anti-T. gondii IgG and IgM antibodies were positive in two (0.6%) women, and 101 (29.7%) women had anti-T. gondii IgG and 70.3% were seronegative. PCR was positive in two IgM-positive women, and both isolates belonged to T. gondii carrying the GRA6 allele of lineage I. The risk of infection was significantly higher in women who had constant contact with cats and soil, and who were residents of rural areas. The two IgM-positive women were asymptomatic regarding acute toxoplasmosis. According to the results of the present study, the prevalence of toxoplasmosis in pregnant women in Urmia is similar to its prevalence in other areas in northwestern Iran, and despite the low prevalence of acute infection, it should not be ignored.

Two new species and some new records of Darwin wasps (Hymenoptera: Ichneumonidae) from northwest of Iran

During a survey of the family Ichneumonidae in northwestern Iran, a total of 17 species belonging to the subfamilies Banchinae, Cryptinae, Ichneumoninae, Mesochorinae, Metopiinae, Orthopelmatinae, Phygadeuontinae and Tersilochinae were identified. Two species, Lissonota (Lissonota) rufofacies Riedel nov. sp. and Orthopelma mukriyana Riedel nov. sp. are new to science and are described and illustrated. Their diagnostic characters are provided. We also provide collecting details for the fifteen species recorded for the first time from Iran.

Trace and minor elements in the ore minerals: An assessment of geochemical distribution in overprinting epithermal on porphyry mineralization at Chodarchay, NW Iran

The Chodarchay overprinting epithermal on porphyry mineralization occurs in the Tarom-Hashtjin subzone of the Alborz magmatic arc in northwestern Iran. Rock units include intrusions (phases I and II) and volcano-volcaniclastic rocks. The alteration types consist of potassic, phyllic, argillic, and propylitic. The porphyry type is mostly accompanied by potassic alteration. The Chodarchay Fault implies two extensional and compressional mechanisms. The porphyry type is associated with the extensional and the epithermal type with the compressional stage of the fault. Mineralization occurred mainly inside the Eocene volcanic and volcano-sedimentary rocks; some ores are associated with the Late Eocene quartz monzonite. The mineralization is divided into three zones from depth to surface: 1) Hypogene sulfide zone (deep); 2) Enriched supergene sulfide zone (near-surface); 3) Oxidized and leached zone (surface). The major sulfide minerals consist of chalcopyrite, pyrite, sphalerite, and galena. Based on the results of the new analyses, chalcopyrite hosts Ag as a solid-solution and contains Ag-rich Bi-sulfosalt micro-inclusions. Non-constant Cd/Zn ratio in the chalcopyrite can indicate varying physiochemical conditions during its crystallization. Low As contents in the pyrite caused relatively clean pyrite with very few elements. The Ni concentrations in the pyrite imply that it was sourced from mantle mafic–ultramafic rocks. Sphalerite is the only Au-host mineral among the sulfide minerals as a nano-mineral phase. Solid-solution Ag exists in the sphalerite. The sphalerite Fe/Zn ratios show that it was precipitated between 220 and 280 °C. Silver and Bi have the highest measured concentrations among all analyzed elements in the galena, mainly partitioning within the galena, but Hg and Cd are abundant in the sphalerite. Bornite is one of the major carriers of Ag and Bi. Silver and Bi exhibit considerable variations in the Cu-(Fe) sulfides. A new occurrence of sulfosalt minerals was identified at Chodarchay (N = 14). The discovered Bi-sulfosalts include cuprobismuthite group minerals (cuprobismuthite, hodrushite, and paderaite), aikinite-bismuthinite series (aikinite and friedrichite), emplectite, makovickyite, galenobismutite, gustavite, heyrovskyite, neyite, cupropavanite; and As-Sb-bearing Cu sulfosalts (watanabeite and colusite). This number of sulfosalts had not previously been reported from any of the Tarom or Alborz deposits or mines. The Au-Ag occurrence was reported from the neyite, emplectite, and gustavite. The Ag-rich minerals crystallize from fractionated fluids that cooled during rising. Silver-carrier sulfosalts may have been exsolution from pre-existing Ag-bearing chalcopyrite in the cooling system. Most of the Ag-bearing sulfosalt minerals formed at the margins and fractures of the chalcopyrite, indicating that the cooling started from these points. Thus, Ag at Chodarchay is hosted inside Cu-(Fe) sulfides, the significant part of them being chalcopyrite and Ag-rich sulfosalt micro-inclusions inside the chalcopyrite. The fluid temperature for the Ag and chalcopyrite precipitation is from 200 to 350 °C. Bismuth assemblages precipitated at > 350 °C. The cuprobismuthite group crystallizes at temperatures > 300 °C. The supergene process and rare secondary enrichment of Ag and Au took place in the oxidation zone; this enrichment is dependent on the presence of manganese. Based on the fire-assay results, the concentration of gold is greater at shallower depths.

Physicochemical Characterization of Novel Bacteriophages of Pseudomonas syringe from Northwest Iran.

Bacterial canker, caused by Pseudomonas syringae, is a devastating disease of stone fruit trees worldwide. The bacterium has a broad host range and a high capacity for adaptation and dissemination, owing to its high mutation rate and horizontal gene transfer. Traditional control methods based on copper compounds and antibiotics have resulted in the development of resistance in the bacterial population. Thus, alternative approaches are needed, such as phage therapy. This study aimed to characterize the physicochemical and biological properties of novel Pseudomonas syringae pv. syringae (Pss)-specific phages isolated from the soils of northwestern Iran. Seventy-five phage isolates were obtained, and their host range was determined against various bacterial pathogens. Five phages exhibiting the highest lytic activity against Pss and a narrow host range were selected for subsequent analysis. The stability of the selected phages was assessed under different conditions such as ultraviolet irradiation, temperature, pH, NaCl concentration, and chloroform exposure. The selected phages demonstrated significant effectiveness invivo, exerting substantial suppression on the population of Pss. This reduction was observed for both individual phages and when the phages were utilized as a mixture. The findings indicate that phages have the potential to be used as biocontrol agents in agriculture.

Molecular Study of Anaplasma spp. in Horses, Sheep, and Goats with Phylogenetic Analysis in Northwest Iran.

Anaplasmosis, a tick-borne disease with worldwide distribution, impacts ruminants, equines, carnivores, and humans. This study aimed to investigate Anaplasma phagocytophilum in horses from Ardabil province and Anaplasma ovis in small ruminants from East Azerbaijan province using the Nested Polymerase Chain Reaction (PCR) method. Blood samples were taken from the jugular vein of 100 healthy horses in the Ardabil province and 156 healthy sheep and goats (116 sheep and 40 goats) in the East Azerbaijan province during the spring and summer seasons of 2016 in northwest Iran. The collected blood samples were stored at -20°C until the molecular experiments were conducted. Nested PCR was employed to detect A. phagocytophilum in horses and A. ovis in small ruminants using extracted DNA and amplifying 16S rRNA and msp4 genes. The Chi-square test of independence was used to determine the relationship between Anaplasma spp., infection, and independent variables, including age, gender, animal species, and sampling location. None of the 100 samples collected from horses in the Ardabil province were positive for A. phagocytophilum. In the East Azerbaijan province, 11 out of the 156 (7.05%) blood samples collected from sheep and goats tested positive for A. ovis. In addition, A. ovisinfection was not significantly related to the independent variables. Phylogenetic analysis showed that the sequence obtained in this study (MH790273) had 100% homology with the sequence obtained from sheep infected with Anaplasma in Ahvaz province (JQ621903.1). The findings of this study can contribute to the prevention and control of anaplasmosis in farm animals in northwestern Iran.

Heightened Smuggling of Weapons and Ammunition from Southern Caucasus to Northwestern Iran on the Eve of World War I

Abstract Documents newly found in the Historical Archives of Azerbaijan Republic reveal that as early as 1912 Ottoman agents were engaged in attempts to entice the Transcaucasian Muslims against Russia and prepare them for the war, which would break out a year-and-a-half later, namely the First World War. Ottomans encouraged the smuggling of Russian weapons and ammunition to regions bordering the Southern Caucasus, especially Northwestern Iran, counting on the sympathy of the Turkophones therein against the Russians, or on their antagonism towards the presence of Russian forces in their own territories. The heightened smuggling of Russian weapons and ammunition alarmed the Russian authorities who began looking into the cases of smugglings in an attempt to prevent or minimize them. Their findings, as expressed in their correspondences, pointed towards an extensive smuggling network headed by an Iranian émigré named Karbalāʾī who ran it with members of his own family and in collaboration with a number of Russian officials.

Incidence of Cancer from 2015 to 2019 in Northwestern of Iran: A Population-Based on Cancer Registry Profile of Maragheh County

Background: Cancer is a leading cause of death globally and poses a significant obstacle to increasing life expectancy in every country, including Iran, where it is a major health threat. Objectives: This study aimed to assess the cancer situation in Maragheh County, Iran, from 2015 to 2019. Methods: Data from Maragheh County for the years 2015 - 2019 were obtained from the Iranian National Population-Based Cancer Registry and the Statistical Center of Iran. The age-standardized rate (ASR) was calculated using the population model suggested by the WHO, with STATA software for data analysis. Results: During the study period, Maragheh recorded a total of 1,700 cancer cases, with males accounting for 970 (54%) of these cases. The average age of cancer patients was 62.4 ± 22.2 years. The ASR for cancer in females ranged from 101.5 to 165.5 per 100,000 people, and for males, it ranged from 128.3 to 150.3 per 100,000 people. Conclusions: The incidence rate of cancer in Maragheh was slightly different from that in Iran and East Azerbaijan province but remained below the global average. The most common cancers in men were stomach, lung, bladder, colorectal, and prostate cancer, while in women, they were breast, colorectal, stomach, leukemia, and thyroid cancer.

Estimation of soil health in the semi‑arid regions of northwestern Iran using digital elevation model and remote sensing data.

Nowadays, neglecting soil conservation issues is one of the most critical factors in reducing soil health (SH). In this regard, to facilitate the estimation of the SH in northwestern Iran, 292 soil samples were taken from a depth of 0-30cm of this area, and a wide range of soil properties were determined. Then, soil health indices (SHIs) were calculated. Simultaneously, the normalized difference vegetation index (NDVI), surface water capacity index (SWCI), and a digital elevation model (DEM) were obtained from satellite data. Finally, multiple linear regression (MLR) relationships between these parameters and SHIs were calculated. In this study, there was a highest significant positive correlation (P < 0.01) between IHI-LTDS and SWCI (0.71**), DEM (0.76**), and NDVI (0.73**). The MLR, with both the whole total (TDS) and minimal (MDS) dataset methods, which includes the aforementioned indices, strongly described the spatial variability of the Integrated Soil Health Index (IHI) (R2 = 0.78, AIC = - 416, RMSE = 0.05, and ρc = 0.76). According to the results of this study, it can be said that the development of SH estimation models using remote sensing extracted parameters can be one of the effective ways to reduce the cost and time of soil sampling in extensive areas.

Two new species of brush-tailed mouse, genus Calomyscus (Rodentia: Calomyscidae), from the Iranian Plateau

Abstract The monogeneric family Calomyscidae includes the brush-tailed mice, genus Calomyscus, which have a Palearctic distribution ranging from the Hindu Kush Mountains in western Pakistan to the eastern Mediterranean region. Zagros Mountains—stretching from northwest to south of Iran—was assumed for long as the range of a single species, Calomyscus bailwardi; however, recent studies revealed considerable heterogeneities among the geographical populations presumably referring to the persistence of undescribed diversity. One such group from the western Zagros Mountains has recently been recognized as C. behzadi Akbarirad, Dezhman, Aliabadian, Siahsarvie, Shafaeipour, and Mirshamsi, 2021. Using molecular, morphometric, geometric morphometric, and karyotypic data, we examine the divergence of 2 additional groups from western Iran and name these as new species. The first of these species is distributed in the northern Zagros and western Alborz Mountains in northwestern Iran was recovered as the sister species to C. urartensis from the South Caucuses. The second species from a single locality in the central Zagros Mountains was clustered closely with C. grandis from the western Alborz Mountains. Morphologic analyses, karyological features, and genetic distances disentangled these 2 new species from their sister species.

The Role of Socioeconomic and Cognitive Status in Determining Traffic Behaviour of Elderly Pedestrians in Iran: A Cross-Sectional Study

Elderly pedestrians are among the most vulnerable groups in terms of traffic-related injuries. This study aimed to investigate traffic behaviour and its determinants among elderly pedestrians in Iran. This cross-sectional study examined the traffic behaviour of 600 elderly pedestrians in Zanjan, Northwestern Iran. The participants were selected using the multistage random sampling method. Pedestrians’ traffic behaviour, socioeconomic status (SES), and cognitive function were assessed using the Pedestrian Behaviour Questionnaire (PBQ), SES questionnaire, and Abbreviated Mental Test score (AMTS), respectively. Multiple linear regression was used to investigate the association of total score of pedestrian traffic behaviour with socioeconomic and cognitive status while adjusting for potential confounding roles of demographic variables. More than 90 percent of elderly pedestrians showed unsafe traffic behaviour. This included traffic violations and failure to adhere to traffic rules. Almost half the elders were aggressive and distracted when walking and crossing the street. A higher total score of PBQ was associated with a higher cognitive status, higher SES, higher levels of education, and walking more than 1 hour a day. The majority of the elders showed unsafe traffic behaviour and elderly pedestrians with high SES and normal cognitive function were more likely to exhibit safe behaviour than those with low SES and cognitive dysfunction. Interventions are needed to improve the traffic behaviour of elderly pedestrians especially those with low SES levels.

Body lice: a vector for re-emerging disease outbreak in a rehabilitation camp in Northwestern Iran

ObjectiveThe report of the outbreak of body louse in northwestern Iran after three decades reminds us again of the danger of the re-emerging of previous epidemics.ResultsThe results of the study that nearly 70% of the patients in a rehabilitation Centre were infected with body louse. In this study, scientific measures were taken to prevent the spread of body lice to healthy people, including isolation of the patients, washing the clothes of those infected at high temperatures, and spraying the rest area, beddings, and blankets. This is a more recent report on an outbreak of body louse in Iran in 2023.

What trees are more suitable for agroforestry implementation? A case study in Northwestern Iran

What trees are more suitable for agroforestry implementation? A case study in Northwestern Iran

The identity of Tanacetum urmiense (Anthemideae, Asteraceae) from northwestern Iran

Tanacetum urmiense Tabad, Maroofi &amp; Rastegar (2022: 112) (Asteraceae) was established on the basis of three collections from Dalanpar Mountain, north-western Iran. The lack of mature achenes led the authors to mistakenly describe this species as a new Tanacetum. In the protologue, the authors stated that the new Tanacetum was related to T. hololeucum (Bornm.) Podlech (1986: 105) but differed in the following characters: plant height (5–12 cm vs. 10–18 cm); indumentum (glabrous or sparsely pubescent vs. densely appressed tomentose); basal leaves dimensions (35 × 12 mm vs. 15–80 × 10–25 mm); capitula diameter (10–20 × 20–30 mm vs. 8–10 × 6–7), and indumentum of the phyllaries (glabrous or sparsely pubescent vs. sparsely or densely hirsutulose).

Prevalence of Helminths Infection in Wild Rodents of Northwestern Iran.

Rodents act as reservoirs, intermediate hosts, or definitive hosts for various zoonotic helminths. Parasitic diseases are among the critical factors affecting the survival and composition of wild rodent populations. Wild rodents share their habitat with domestic free-grazing animals, mainly sheep, stray dogs, and cats, which allows the transmission of helminth infections, such as Toxocara and Trichinella, to these animals. This study investigated the helminth parasite fauna of wild rodents in East Azerbaijan province, north-western Iran, and discussed the possibility of parasite transmission among wild rodents. A total of 204 rodents of 17 different species (spp.) were collected in north-western Iran. Information about the genus, developmental stage, and rodent spp. was recorded for each animal. The gastrointestinal tract, liver, and diaphragm were examined for the presence of helminths. The recovered specimens were identified based on references. The prevalence rate of helminth infection among the captured rodents was 67.16%. Meriones persicus (M. persicus) showed the highest infection and diversity rates. This spp. harbored the zoonotic helminth Capillaria hepatica and some spp. belonging to the genera Toxocara, Syphacia, and Hymenolepis. M. persicus specimens from mountainous areas showed the highest infection rate. This study aimed at evaluating the potential role of wild rodent spp. as reservoirs of helminth infection in East Azerbaijan province, Iran. M. persicus was the most common spp. in our study and represented a higher proportion of the infected rodents in terms of helminth diversity and frequency. Harboring zoonotic helminths, M. persicus represents a health risk. Further studies are recommended to evaluate the prevalence of these parasites in the human community and inform people concerned about the risk of disease transmission to humans through rodents.

Effect of environmental, seed burial depth, and straw mulch on germination and seedling emergence in Cichorium glandulosum

AbstractCichorium glandulosum Boiss. et Huet is a species that has recently spread widely in the autumn crops of northwestern Iran. A study was conducted to evaluate the effect of environmental factors on the germination, emergence, and management of two populations of C. glandulosum. The effects of temperature, photoperiod, NaCl concentration, osmotic potential, seed burial depth, and straw mulch on seed germination and seedling emergence were evaluated for two populations of C. glandulosum from Tabriz and Marand, Iran. The highest germination percentage was observed in the Tabriz (93%) and Marand populations (94%) at 20/10 C (day/night). In both populations, germination was 82% to 93% across a wide range of light/dark periods (8 to 24 h of light). However, germination was significantly reduced (∼70%) under continuous darkness. The osmotic potential required to inhibit 50% of germination was 0.68 MPa for the Tabriz population and 0.62 MPa for the Marand population. The concentration of NaCl required to inhibit 50% of germination was 4.76 dS m−1 for the Tabriz population and 3.81 dS m−1 for the Marand population. The seed burial depths that caused a 50% decrease in emergence for the Tabriz and Marand populations were 1.86 cm and 2.22 cm, respectively. In the Tabriz and Marand populations, the application of 6000 kg ha−1 of straw mulch resulted in a decrease in C. glandulosum emergence to 3% and 10%, respectively. This study’s results inform the conditions required for C. glandulosum germination and establish a theoretical and practical foundation for predicting, preventing, and managing this species using scientific principles.

Nursing Students’ Competency about Writing Nursing Care Plan: An Exploratory Study in Iran

Background. By utilizing the nursing care process, healthcare professionals can implement nursing knowledge effectively and efficiently, ultimately leading to a significant improvement in the quality of care provided. Objective. This study aimed to assess writing nursing care plan competence among Ardabil nursing students in northwestern Iran. Methods. The study involved 248 nursing students from the second to eighth semesters in three nursing and midwifery schools located in the Ardabil province of northern Iran. The data were collected using a simple random sampling method and included a demographic questionnaire along with a survey questionnaire on nursing care plan writing skills. This was conducted between May and June 2023. Descriptive statistics, Pearson correlation, t-test, ANOVA, and multiple linear regression analyses were conducted for data analysis. Results. The mean score (SD) for writing a nursing care plan in a student survey was 3.35 (0.57) on a scale of 1 to 5. The survey’s weighted mean score for each dimension is as follows: data gathering (D) = 3.40 (0.73), identification of client’s problems (P) = 3.40 (0.73), sustainable goals (G) = 3.31 (0.77), appropriateness of intervention (I) = 3.30 (0.67), and recognizing outcomes (O) = 3.37 (0.69). This indicates that the students performed well overall. However, the lowest score was in the appropriateness of interventions dimension. Multiple linear regression analysis revealed that four variables, mean score of grade point average (GPA), age, academic term, and time spent studying (hours per day), were significant predictors of the student survey’s scores on writing nursing care plans. These variables could predict 61% of the total variance. Implication for Nursing Management. Nursing school administrators can establish programs to improve education and practice in nursing care plan development based on this study. They can use these findings to enhance nursing education and practice for students, which will ultimately lead to better nursing care plans. Administrators should ensure that students have the necessary skills for writing efficient care plans and provide training courses to improve their writing and critical thinking skills. Policies can be implemented to encourage students to increase their study hours and maintain quality care programs. Conclusion. Nursing students are skilled in creating well-written nursing care plans that follow the nursing process. The quality of these plans improves with higher GPAs and longer study hours. To enhance nursing care plans, nursing education should focus on developing skills and allocating sufficient time for studying and practicing. Collaborating with faculty and administrators, as well as using reliable resources, can also help improve the quality of care plans.

Comparative molecular epidemiology, subtype distribution, and zoonotic potential of Blastocystis sp. in Equus animals (horses, donkeys, and mules) in northwestern Iran

A total of 500 fecal samples were collected from Equus animals in six different cities (Ardabil, Namin, Nir, Meshginshahr, Germi, and Khalkhal) of Ardabil Province, northwestern Iran, with 200 samples from horses, 200 from donkeys, and 100 from mules. Of the horse samples, 100 were from racing horses under special monitoring and care, while the remaining 100 were from non-racing horses, including those used for herding or in rural areas. All fecal samples were examined for the presence of Blastocystis sp. using PCR amplification of the SSU rRNA gene's barcode region after DNA extraction. The molecular prevalence of Blastocystis infection in Equus animals was 7.6% (38/500). Blastocystis was more common in horses [11.5% (23/200)] than in donkeys [5.5% (11/200)] and mules [4% (4/100)] (P > 0.05). Compared to racing horses [3% (3/100)], non-racing/rural horses [20% (20/100)] exhibited a substantially higher prevalence of Blastocystis (P < 0.05). The prevalence of Blastocystis in diarrheal samples and younger animals was remarkably higher than in formed samples and older animals, respectively (P < 0.05). No significant difference in Blastocystis infection prevalence was found between the genders of examined animals (P > 0.05). In Equus animals, 38 Blastocystis isolates included eight STs: ST10 [31.6% (12/38)], ST1 [21.1% (8/38)], ST2 [15.8% (6/38)], ST3 [10.5% (4/38)], ST4 [7.9% (3/38)], ST7 [5.2% (2/38)], ST14 [5.2% (2/38)], and ST6 [2.6% (1/38)]. These results suggest that Equus animals act as a proper reservoir for numerous Blastocystis STs, consequently playing a crucial part in the spread of this protozoan infection to humans, animals, and water reservoirs.

Characteristics of suicide attempts in Northwestern Iran: a five-year population-based survey.

Suicide is a serious public health problem in the world. This study aims to describe the characteristics of suicide attempters in North-Western Iran and identify latent classes of suicide attempts. This cross-sectional study was conducted in Ardabil Province (Northwest Iran) during 2017-2021 based on a registration system for suicide attempts. We performed latent class analysis (LCA) using a procedure for LCA (PROC LCA) in SAS to investigate the subgroups of suicide attempters based on their characteristics and method, history, and outcome of suicide. Three latent classes were identified for males and females; the first class (non-lethal attempters with lower educational levels) comprised 41.3% of males and 55.4% of females. The second class (non-lethal attempters with higher educational levels) described 52.4% of males and 42.7% of females. Finally, the third class (lethal attempters) included 6.4% of males and 1.9% of females. The main method of suicide attempts was poisoning with medications (87.3%). The results show that only 2.8% of people have a history of suicide attempts. Also, the suicide rate reached 8.26 per 100,000 population in 2021. The present study showed an increasing trend of suicide attempt incidence rate in Ardabil Province from 2017 (99.49 per 100,000 population) to 2021 (247.41 per 100,000 population). This means that the rate of change was 147.92 per 100,000 population during the study period. The findings of LCA, stress the necessity of identification and prioritization of unmet needs of people who had an incomplete suicide in Ardabil.