Northwest Romania Research Articles

The Impact of Agricultural Inputs and Environmental Factors on Potato Yields and Traits

Potato, a component of global food security and economic stability, is cultivated extensively worldwide due to its adaptability to diverse climates and soil types. Ongoing research and technological advancements, including the use of unconventional products destined to fight the most harmful pathogens, are essential for enhancing productivity and resilience in potato farming. The current study aimed to identify the most appropriate fertilizers and phytosanitary treatments in order to attain optimal potato yields, dry matter, and starch contents, under climate-specific environments. The experiment was conducted in the northwest of Romania in 2023. The research was organized as a trifactorial experiment with the factors variety, fertilization, and phytosanitary treatment. XLSTAT (2022.2.1v.) was utilized for data processing. Mineral fertilization combined with conventional phytosanitary treatment led to the highest average yields in both potato varieties. Also, a combination of organic fertilization and treatment with 4% A. cepa extracts led to notable results, which suggests the possibility of successfully using these inputs in organic agriculture. The study shows the importance of selecting appropriate agricultural inputs to optimize potato yields, achieve specific levels of dry matter and starch content in a specific area, and promote sustainability.

Toxoplasma gondii genotypes and frequency in domestic cats from Romania

BackgroundToxoplasma gondii is a zoonotic protozoan parasite with a heteroxenus life cycle that involves felids as the definitive hosts and any warm-blooded animal, including humans, as intermediate hosts. Cats are key players in parasite transmission as they are capable of shedding high numbers of oocysts in their feces that contaminate the environment.MethodsThe study was performed on 31 domestic cats (31.23 ± 27.18 months old) originating from rural and urban areas (5.17:1) in the center and north-west Romania. Feces (n = 31), blood (n = 28), and heart samples (n = 27) were collected. Fecal samples were analyzed by flotation technique, and PCR (529 bp repetitive element). Fecal samples with T. gondii oocysts were bioassayed in mice. Serum samples were analyzed by modified agglutination test and ImmunoComb for the detection of specific anti-T. gondii IgG antibodies. Heart samples were bioassayed in mice, and analyzed by PCR. Toxoplasma gondii positive samples were genotyped by nPCR-RFLP targeting eleven genetic loci (SAG1, SAG2, alt-SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico).ResultsToxoplasma gondii oocysts were found in 2 out of 31 fecal samples collected from a 3-months old stray kitten, and a 4-years old female. In total, 17 out of 27 sera were positive for T. gondii IgG antibodies. The antibody titers in MAT ranged from 1:6 to 1:384. Toxoplasma gondii DNA was detected in 7 out of 27 heart samples, and four of them were positive also by bioassay. Six T. gondii DNA samples from bioassayed mice could be assigned to ToxoDB PCR-RFLP genotype #1 or #3 (Type II) and one T. gondii DNA from heart digest to genotype #2 (Type III). Both of these genotypes are common in Europe.ConclusionsOur results revealed that the infection with T. gondii is still high in cats from Romania. The oocysts shedded by these cats represent an important source of infection for intermediate hosts, including humans. Further studies on a wider range of cases are necessary for a more exhaustive definition of the T. gondii genotypes circulating in Romania.

Comparative assessment of different rose cultivars under environmental conditions in central Transylvania, Romania

Despite the abundance of rose varieties, it is necessary to create and use new genotypes to improve the appearance of green spaces and meet the demands of society, as well as to ensure their response to environmental variables and climate change. To assess the overall value and flower quality of several Romanian and foreign varieties under the ecological circumstances of Northwest Romania, 14 rose varieties from the shrub and floribunda groups were examined. The study identified notable variations among the cultivars for plant growth, foliage, flowers, and their susceptibility to the three primary fungal diseases (black spot, powdery mildew, and rust) prevalent in the study area. Additionally, the study identified the cultivars with proper resilience to frost during the five-year investigation period, particularly after the cold winters. New varieties obtained at HRS Cluj-Napoca, such as Cristiana, Pasiune mov, Maria-Cristina and Splendid, and international varieties such as Fisherman's Friend, Bonica and Mirato, stood out for their decorative quality and attractive floral elements. Some of these varieties have also been considered potential parents for disease and frost resistance in new hybridization projects. Broad-sense heritability data for the quantitative variables under investigation, as well as multivariate analyses, provided useful information for new rose breeding programs. The comparison of quantitative and qualitative assessments of important decorative traits for the roses, together with proper statistical calculation processes to assure their validity and trustworthiness, enabled the development of hypotheses or working models that can improve rose breeding efficiency.

Trichinella spiralis Infecting Wild Boars in West, Southwest, and Northwest of Romania: Evidence of an Underrated Risk.

The species of the genus Trichinella are etiological agents distributed all over the world and are able to infect mammals, birds, and reptiles. Trichinella spiralis is the species most adapted to domestic and wild pigs and is also the most important etiological agent of trichinellosis. The wild boar (Sus scrofa) is a nocturnal omnivorous mammal belonging to the Suidae family. S. scrofa has a great appetite and its diet includes a variety of small prey such as mice, rats, and other rodents, as well as carcasses of larger animals. The aim of this study was the identification and the molecular characterization of Trichinella larvae isolated from the muscle tissue of S. scrofa specimens collected in different counties of Romania. The muscle samples were examined by artificial digestion and the larvae identified at the species level by multiplex PCR. T. spiralis, a species that is able to infect a considerable number of different host species including humans, was identified. In Romania, S. scrofa is an important reservoir species for T. spiralis and plays an important role in linking the domestic and the wild cycle of Trichinella, with serious repercussions for human health.

The use of genetic resources in rose breeding and creation of new rose cultivars through hybridization and selection

Despite the existence of more than 37,000 different varieties of roses worldwide, there remains a significant interest and high relevance in rose breeding. The rose is the most beloved flower in the world, commonly named the “queen of flowers”. In addition to its remarkable ornamental significance, the rose possesses various ecological, environmental, landscaping, food, pharmaceutical and medicinal, cosmetic, and industrial attributes, among others, thereby contributing to its overall socio-economic value. The current study investigated the results of rose breeding in a horticulture research center in the northwest of Romania with over 70 years of experience. The main method used to generate variability for the selection of new genotypes was artificial hybridization. After 1970, an average of 15-20 crossings per year were carried out, in different parental formulas or types of crosses, with approximately 1000-2000 flowers per pollinated per parental combination. Thousands of F1 hybrids were obtained annually (approximately 10,000-12,000 hybrids per year per hybrid combination), and dozens of elites were selected, grafted, and promoted as clonal selections in comparative trials. The success rate in obtaining new varieties through hybridization was 5.1% from total hybrid combinations, 0.72% from total hybrids, and 1.6% from total clonal selections. Of the total new varieties, hybrid teas represent 11.9%, floribundas 64.2%, shrubs 16.6%, and others 7.1%. 38 new cultivars suitable for open field cultivation were obtained, showing different habits, decorative value, flowering behavior, flower color, fragrance, resistance to frost or major diseases, and intended use. The exploration of new research directions aimed at harnessing the potential of roses, the region, and the socio-economic requirements was undertaken to enhance and beauty degraded, or landslide-prone lands, as well as to introduce damascene roses for the oil perfume and cosmetics industry.

Evaluating Groundwater Metal and Arsenic Content in Piatra, North-West of Romania

The present study introduces a monitoring initiative focused on the quality of groundwater in the Piatra locality, situated in the North-West region of Romania. This paper employs an evaluation of 21 physico-chemical parameters, encompassing factors such as electrical conductivity, pH, chemical oxygen demand, turbidity, total hardness, NH4+, NO3−, Cl−, PO43−, Li, Na, K, Ca, Mg, Ba, Sr, Al, Fe, Mn, Sn, and Ti. Additionally, it examines five heavy metals (Cr, Cu, Ni, Pb, and Zn) and arsenic in water sourced from six distinct private wells. Each well, with its characteristics, serves as a unique drinking water source. The assessment encompassed the evaluation of pollution levels, quality status, and risk factors for all drinking water sources, utilizing pollution, quality, and risk indices. The aim of this study was to establish the level of toxicity in water, assess its impact on human health, and disseminate information to the public about the appropriate utilization of individual water sources. The results indicated a general contamination with chloride, ammonium, manganese, chromium, and iron. Human health risk assessment indices revealed that the consumption of studied waters presented non-carcinogenic risks associated with Cr for adults and with Cr, As, Pb, and Cu for children for some of the groundwater sources. The water quality index (WQI) categorizes the samples as possessing excellent and good quality. This research represents one of the initial endeavors to assess the groundwater source quality in connection with the potential human health risks posed by the metals studied within the protected area of the Tisa River Basin.

Histopathology Features of H. pylori Gastritis Associated With Altered Lipid Profile: An Observational Study from a Tertiary Healthcare Center in North West Romania.

H. pylori infection can promote a systemic inflammatory syndrome, eventually leading to intestinal metaplasia and gastric cancer. The aim of our study was to investigate the possible association between dyslipidemia and histopathological features of H. pylori gastritis. An observational, retrospective study was conducted over the period 2017-2022 on symptomatic patients with a positive rapid urease test. A total of 121 patients who underwent upper gastrointestinal endoscopy with stomach biopsy were enrolled in this study. Based on the updated Sydney System, we investigated the association between neutrophils, mononuclear cells, intestinal metaplasia, or gastric atrophy and altered lipid profiles. A high prevalence of H. pylori infection was noticed in the studied group upon the application of the rapid urease test, being associated with dyslipidemia regardless of patient sex. All the endoscopic diagnoses (acute, chronic, or atrophic chronic gastritis, metaplasia) correlated with the histopathological features. Mononuclear cells and metaplasia were more likely to be found in H. pylori-positive patients with dyslipidemia, which is consistent with acute and chronic inflammation caused by H. pylori in the gastric mucosa. Although our study was conducted on a small scale, it offers new insights and details regarding H. pylori infection and histopathological features. Mononuclear cells and metaplasia were associated with an altered lipid profile in H. pylori-positive patients. These findings warrant future investigation, such as the evolution of gastric biopsies and lipid profiles before and after eradication.

THE INFLUENCE OF VARIETIES, FOLIAR FERTILIZATION AND BIOSTIMULATOR ON TRITICALE PRODUCTION IN NORD-WESTERN ROMANIA

Triticale (Triticosecale Wittmack) are a hybrid created by man by crossing two species, namely Triticum aestivum and Secale cereale. They adopted the good ability to adapt to climatic conditions, high resistance to plant diseases and the utilization of low fertility soils from Secale cereale and the high production capacity and high protein content from Triticum aestivum. In general, triticale is used in feeding animals in different forms (concentrated fodder, straw, silage, pasture), especially in feeding pigs and birds. More recently, it was discovered that this plant has several properties, being similar to wheat. It is also used in the bioethanol, alcohol, cellulose industry and even in aquaculture. Fertilization is one of the most important in the cultivation technology of this plant, ensuring production increases both quantitatively and qualitatively. The experiment is located in the year 2021-2022, in northwest Romania, in Livada, Satu Mare county on a typical preluvosol with an acidic reaction, with a pH of 5.19 in the arable layer. It is placed after the method of subdivided plots, being an experiment with three experimental factors, of which only two factors were studied, the variety and fertilization and their influence on triticale production. Due to the severe climatic conditions, with high temperatures and lack of precipitation, additional fertilization with foliar fertilizer and biostimulator recorded significantly negative differences in production. It was found that the best fertilization option during that testing period was the basic fertilization of the soil with complex fertilizers in the fall and nitrogen-based fertilizers in the springtime.

Airplane Parts and Covid Masks: Labour Commuters of North-Western Romania Between Central and Eastern European “Re-Industrialisation” and the Global Market

Abstract This article aims to uncover two main features of ‘re-industrialisation’ in Central and Eastern Europe: the reconfiguration of the economic geography in Northwest Romania and the multiple ways in which the Romanian working class is being integrated into the new economy. Post-socialist shifts towards a low-skilled, flexible, and generally insecure economy have underlined the need for cheap, easily disposable labour, and the emergence of the new economic geography has changed the accumulation of capital in the region and the patterns of labour mobility. Despite massive migration, many have continued to work in the region or have combined migration periods with work close to home. This study explores the different mobilities individuals engage in and seeks to understand why some workers choose to stay and live in the region and how the available opportunities for workers aiming to stay in the region influence their prospects. This study traces the patterns of labour commuting and how this is structured by individuals’ strategies and motivations, as well as the social relationships that support this work. The article analyses labour commuting to two major industrial hubs in the region: one which manufactures aerospace components, and one that produces medical textiles. Both companies are foreign-owned and concentrate a significant proportion of the region’s workforce. The micro-dynamics revealed will contribute to understanding the patterns of work in the specific form of re-industrialisation in contemporary Romania.

Labour Force Composition and Labour Shortage in North-Western Romania: A Cross-County Comparison1

Abstract The paper analyses the labour force composition of two adjacent counties in north-west Romania: Maramureș and Sălaj. Regionally, employers stress the lack of available labour force and resort to commuter networks from nearby rural areas and immigrant labour. Why labour shortage? It is argued that Romania’s FDI-reliant export-led growth model factors in. Namely, the growth model’s reliance on low-cost labour that reduces employment incentives to a minimum (often minimum wage) and employment in repetitive labour-intensive activities make the prospect less attractive. If technological upgrading – requiring skilled employees – is absent, regional labour availability tends to be an issue. Alternative subsistence methods are favoured: seasonal transnational migration, household agricultural subsistence and remittances from relatives. Tying livelihood to families and households, these methods pool resources to replace (even if in part) wage labour under global market-dependency conditions.

Knowledge, Attitudes, Intentions and Vaccine Hesitancy among Postpartum Mothers in a Region from the Northwest of Romania.

This study aims to identify the presence of vaccine hesitancy and the factors that could have determined it in a group of mothers in the postpartum period, with an evaluation of both the level of knowledge and information, as well as the attitudes, perceptions, intentions and sources of information about vaccination. The study was based on a survey-Vaccine Hesitancy Identification Survey-applied in two maternity wards from Bihor County and structured into six subscales (34 items). Based on the answers to the key questions ("Which of the following statements best describes your plans for vaccinating your child?"-item 1 of subscale 4; "Overall, how hesitant do you consider yourself to be about vaccinating your child?"-item 4 of subscale 4), we identified two groups: the group of mothers without hesitant behavior (non-hesitant), called the group pro vaccine (GPV), and the group of mothers with hesitant behavior, called the group non vaccine (GNV). Vaccine hesitancy was identified in our study in 47.28% of the participants (191 of the 404 mothers included). Most of them come from an urban environment (57.59%), have university and post-secondary education (58.64%) and are prim parous (58.64%). The behavior of participants from GNV is influenced by a low level of knowledge and information regarding vaccination and by concerns related to adverse reactions, new vaccines and the number of vaccines administered. Also, this group is characterized by an increased perception of the risks related to vaccination, while the perception of the risks associated with the disease is low. For all subscales, important differences were registered between the two groups in favor of GPV, a group characterized by positive attitudes and perceptions and a better level of knowledge compared to GNV. This study aims to represent a starting point for the organization and running of information campaigns regarding vaccination at the level of Bihor County, especially in areas with low vaccination coverage, where this behavior is identified.

Prevalence of comorbidities and survival analysis of COVID-19 patients – an observational study from a tertiary healthcare center in North West Romania

Prevalence of comorbidities and survival analysis of COVID-19 patients – an observational study from a tertiary healthcare center in North West Romania

Evaluation of the Knowledge and Attitude of Adolescents Regarding the HPV Infection, HPV Vaccination and Cervical Cancer in a Region from the Northwest of Romania.

In Romania, after reaching the age of 18, teenage girls can make an informed decision about vaccination. The aim of the study was to evaluate both knowledge and attitudes related to HPV infection, HPV vaccination, cervical cancer, as well as intentions, reservations, reasons that could influence the decision related to vaccination. We applied an anonymized questionnaire to 690 teenage girls in the 18-19 age group, recruited from 15 high schools in Bihor County. Based on the answers to the question corresponding to item 14 of subscale 3, 2 groups were identified: the group with a hesitant behavior called the non-vaccine group (GNV) and the group with a positive behavior called the pro-vaccine group (GPV). The statistical analysis was processed by using IBM-SPSS 22. The analysis revealed significant differences between the groups in terms of the level of knowledge and attitudes related to vaccination, the adolescent girls in the GPV being more aware of the role and importance of HPV vaccination and more open to the idea of vaccination. For the GNV, the behavior can be explained by the fact that even if they have heard about the HPV infection being transmitted through unprotected sex and they think that vaccination in general is necessary to prevent certain diseases, they do not know if HPV vaccination would be effective in protecting them in the future. Adolescents from GPV declare they would accept HPV vaccination if it would be offered to them or they are already vaccinated. Alongside interventions targeting parents and health-care professionals, it is necessary to improve the level of knowledge of adolescents about HPV infection, HPV vaccination and cervical cancer, by organizing information campaigns in schools, campaigns in which professionals in the field should be involved, but also by implementing education programs addressed to them.

PRELIMINARY RESULTS REGARDING THE OPTIMAL SEED RATES OF SOME WINTER WHEAT VARIETIES

Before to extended on large surfaces, the new wheat varieties need to be examinate to finding their requirements regarding technological components, like planting density. In accord with this demand, during the agricultural year 2021 – 2022 were tested at Oradea, in north-west of Romania, our news registered varieties Dacic and Biharia, beside of other 23 Romanian or foreign varieties of wheat, at seven seeding rates:200, 300, 400, 500, 600, 700 and 800 seeds/m2. Statistical processing of the yield and wheat features were dan by usual correlation (Pearson), Spearman rank correlations, the calculations of linear and quadratic equations of correlations between grain yield and seeding rate and calculation of linear, quadratic, exponential and logarithmic trend between the same characters, the trend of the most significant determination coefficient being utilised in graphical transpositions. Some genotypes (Voinic, Bogdana, Dacic, Cezara and Crisana) have their best ranks of yield at low densities while other (Gabrio, Consecvent, Anapurna and Abundent) at the greatest ones. Taking in consideration the average of ranks, the genotype Consecvent has the best average position, followed by Gabrio, Abundent, Bogdana and Voinic. These five genotypes have a good capability to adjust their yields components to vary densities of plants. At low densities (200, 300, 400 and 500 germinal seeds/m2), the genotypes yield ranks are comparable but at high densities (more than six hundred seeds/m2), the ranks are stronger effected by density. Every genotype has an optimum seed density, depending on its capacity to tolerate or no high density: Crisana- 500 seed/m2, Biharia- 600 seed/m2, Consecvent- 600 to 700 seed/m2, Anapurna- 800 seed/m2, etc. Some varieties, like Voinic, have a pour response to seeding rate, they yielded well even at small density, being able to compensate the reduced density by tillering capacity, number of grains/spikes, better test weight and bigger grain size.

Monitoring Forest Cover Dynamics Using Orthophotos and Satellite Imagery

The assessment of changes in forest coverage is crucial for managing protected forest areas, particularly in the face of climate change. This study monitored forest cover dynamics in a 6535 ha mountain area located in north-west Romania as part of the Apuseni Natural Park from 2003 to 2019. Two approaches were used: vectorization from orthophotos and Google Earth images (in 2003, 2005, 2009, 2012, 2014, 2016, 2017, and 2019) and satellite imagery (Landsat 5 TM, 7 ETM, and 8 OLI) pre-processed to Surface Reflectance (SR) format from the same years. We employed four standard classifiers: Support Vector Machine (SVM), Random Forest (RF), Maximum Likelihood Classification (MLC), Spectral Angle Mapper (SAM), and three combined methods: Linear Spectral Unmixing (LSU) with Natural Breaks (NB), Otsu Method (OM) and SVM, to extract and classify forest areas. Our study had two objectives: 1) to accurately assess changes in forest cover over a 17-year period and 2) to determine the most efficient methods for extracting and classifying forest areas. We validated the results using performance metrics that quantify both thematic and spatial accuracy. Our results indicate a 9% loss of forest cover in the study area, representing 577 ha with an average decrease ratio of 33.9 ha/year−1. Of all the methods used, SVM produced the best results (with an average score of 88% for Overall Quality (OQ)), followed by RF (with a mean value of 86% for OQ).

Child-Centered Approach through Slow Education Principles: A View to Child Personality Development in Early Childhood

We are preoccupied with early childhood education policies at the international level in general, but in particular with early childhood education in Romania. Therefore, the research direction of our study focuses on how early education teachers can integrate the principles of slow education into the development of child-centered competence. The formation and development of the child’s early ontogeny personality occur at the natural pace of development and learning rather than in an accelerated manner. While preparing for the research, we reviewed relevant articles circumscribed to the same topic: slow, child-centered education, deep learning, constructivism, and the enthusiasm of children and teachers. Most of them conclude that the use of child-centered competence in early childhood significantly contributes to slow education principles and characteristics. At the same time, the research aims to provide quantitatively and qualitatively validated arguments for verifying the incidence of child-centered competence from the perspective of sustainable education, in connection with the constructivist orientation of the teachers. Our research included a group of 376 teachers selected from early childhood education institutions in the North-West of Romania, all of them with initial preparation in the field. Using the focus group method, we identified differences in the perception of the two paradigms (child-centeredness and slow pedagogy), the link between these two, and the connection with the constructivist orientation. At the same time, we intended to identify the degree of continuous professional development training according to these paradigms that are not studied and practiced together in early education in Romania, because only the child-centeredness principle is mentioned in the initial teacher training. Therefore, in-service training activities were conducted and they were focused on facilitating and raising awareness of the value of using child-centered competence and the principles of slow education among training participants. At the end of the program, through the quantitative method—the survey—we concluded that the activities included in the training program led to a significant increase in the teachers’ capacity to appreciate the natural pace of development and learning of children to such an extent that the participants themselves adopted the promotion of sustainable slow education in a dynamic society. The methods used helped us deduce that all teachers with a constructivist orientation improved their child-centered competence, and teachers who gave children the freedom to choose activities from the proposed educational offers, according to their needs, interests, and potential, identified the development of the ability to consider children’s natural pace of development and learning as a result of the training. In conclusion, our research complements the literature, proposing a new and useful perspective to approach early childhood education practices by respecting the child’s natural pace of development and learning, which provides a suitable context for promoting international sustainable educational policies. We encourage teachers around the world to reflect on an important issue in the holistic development of the child—if faster means better—in the race to performance.

The Motivation of Medical Staff and the Work Interestedness in the Context of the COVID-19 Pandemic, in a Tertiary Hospital in Romania.

The purpose of this study was to evaluate the impact of the COVID-19 pandemic on the motivation and work interestedness of employees in a tertiary hospital located in the northwest of Romania. In the study, 2230 employee satisfaction questionnaires distributed during 2019-2021 in the Oradea Emergency County Clinical Hospital (CCEHO) were analyzed. The percentage of those who declare themselves motivated at the hospital level remains relatively constant, at around 75%. There were differences between staff categories. The percentage of those who evaluate work interestedness with the qualifier "high" decreased from 45.45% for the year 2019 to 41.78% for the year 2021. The degree of motivation and work interestedness showed a significant increase for TESA staff during the pandemic period compared to the year 2019. A non-significant statistical increase in the percentage of motivated staff was observed among physicians, auxiliary staff and the radiology department staff. The motivation of nurses and laboratory staff decreased, but statistically insignificantly. The COVID-19 pandemic brought statistically significant changes at the level of motivation of the hospital staff only for the administrative staff, and the work interestedness for physicians and nurses decreased statistically significantly, especially in the second year of the pandemic. Older staff with an average level of education are more likely to be unmotivated, as shown by the regression model.

Epidemiological Study on Spinal Cord Injuries in a Hospital from North-West of Romania

Epidemiological Study on Spinal Cord Injuries in a Hospital from North-West of Romania

Non-Syndromic Hearing Loss in a Romanian Population: Carrier Status and Frequent Variants in the GJB2 Gene.

The genetic causes of autosomal recessive nonsyndromic hearing loss (ARNSHL) are heterogeneous and highly ethnic-specific. We describe GJB2 (connexin 26) variants and carrier frequencies as part of our study and summarize previously reported ones for the Romanian population. In total, 284 unrelated children with bilateral congenital NSHL were enrolled between 2009 and 2018 in northwestern Romania. A tiered diagnostic approach was used: all subjects were tested for c.35delG, c.71G>A and deletions in GJB6 (connexin 30) using PCR-based methods. Furthermore, 124 cases undiagnosed at this stage were analyzed by multiplex-ligation-dependent probe amplifications (MLPA), probe mix P163, and sequencing of GJB2 exon 2. Targeted allele-specific PCR/restriction fragment length polymorphism (RFLP) established definite ethio-pathogenical diagnosis for 72/284 (25.35%) of the cohort. Out of the 124 further analyzed, in 12 cases (9.67%), we found compound heterozygous point mutations in GJB2. We identified one case of deletion of exon 1 of the WFS1 (wolframin) gene. Carrier status evaluation used Illumina Infinium Global Screening Array (GSA) genotyping: the HINT cohort-416 individuals in northwest Romania, and the FUSE cohort-472 individuals in southwest Romania. GSA variants yielded a cumulated risk allele presence of 0.0284. A tiered diagnostic approach may be efficient in diagnosing ARNSHL. The summarized contributions to Romanian descriptive epidemiology of ARNSHL shows that pathogenic variants in the GJB2 gene are frequent among NSHL cases and have high carrier rates, especially for c.35delG and c.71G>A. These findings may serve in health strategy development.

Phytochemical Profile and Antioxidant Capacity of Viscum album L. Subsp. album and Effects on Its Host Trees.

Viscum album L. subsp. album is a hemiparasitic plant that is recognized as a medicinal plant due to its beneficial effects, including anti-tumor activity, antioxidant, anti-inflammatory, anti-hepatotoxic, hypoglycemic, and antimicrobial properties as well as for lowering blood pressure. On the other hand, mistletoe is a biotic stressor for both deciduous trees and conifers. Our main aim was to evidence the influence of mistletoe on the content of chlorophylls, proline, total phenols, flavonoids, and antioxidant capacity of leaves from tree host trees (Malus domestica, Prunus domestica, and Populus alba) that grow on the northwest of Romania. In addition, HPLC-DAD-MS-ESI+ was used to analyze the phenolic acid and flavonoid profiles of V. album L. subsp. album leaves according to their parasitized hosts. A significant decrease in chlorophyll a level of approximately 32% was detected in poplars infested with mistletoe, followed by infested apples and plums with pigment reductions of 29.25% and 9.65%, respectively. The content of total phenols and flavonoids in the parasitized trees was higher compared to the non-parasitized ones. In the case of poplar, which presented the highest incidence of mistletoe infestation (70.37%), the content of total phenols in the leaves was two times higher compared to non-infested leaves. Based on HPLC chromatographic analysis, leaves of mistletoe growing on apple (VAM) had the highest content of phenolic acids (7.833 mg/g dw), followed by mistletoe leaves on poplar (VAO) and plum (VAP) (7.033 mg/g dw and, respectively, 5.559 mg/g dw). Among the flavonols, the predominant component was Rhamnazin glucosides in the amount of 1.025 ± 0.08 mg/g dw in VAO, followed by VAP and VAM (0.514 ± 0.04 and 0.478 ± 0.04 mg/g dw, respectively). Although our results show that mistletoe negatively influences the host trees, it is still a valuable plant that must be exploited to bring benefits to human health.