Northern Sites Research Articles

Enterovirus detection in different regions of Madagascar reveals a higher abundance of enteroviruses of species C in areas where several outbreaks of vaccine-derived polioviruses occurred

BackgroundPoliomyelitis outbreaks due to pathogenic vaccine-derived polioviruses (VDPVs) are threatening and complicating the global polio eradication initiative. Most of these VDPVs are genetic recombinants with non-polio enteroviruses (NPEVs) of species C. Little is known about factors favoring this genetic macroevolution process. Since 2001, Madagascar has experienced several outbreaks of poliomyelitis due to VDPVs, and most of VDPVs were isolated in the south of the island. The current study explored some of the viral factors that can promote and explain the emergence of recombinant VDPVs in Madagascar.MethodsBetween May to August 2011, we collected stools from healthy children living in two southern and two northern regions of Madagascar. Virus isolation was done in RD, HEp-2c, and L20B cell lines, and enteroviruses were detected using a wide-spectrum 5ʹ-untranslated region RT-PCR assay. NPEVs were then sequenced for the VP1 gene used for viral genotyping.ResultsOverall, we collected 1309 stools, of which 351 NPEVs (26.8%) were identified. Sequencing revealed 33 types of viruses belonging to three different species: Enterovirus A (8.5%), Enterovirus B (EV-B, 40.2%), and Enterovirus C (EV-C, 51.3%). EV-C species included coxsackievirus A13, A17, and A20 previously described as putative recombination partners for poliovirus vaccine strains. Interestingly, the isolation rate was higher among stools originating from the South (30.3% vs. 23.6%, p-value = 0.009). EV-C were predominant in southern sites (65.7%) while EV-B predominated in northern sites (54.9%). The factors that explain the relative abundance of EV-C in the South are still unknown.ConclusionsWhatever its causes, the relative abundance of EV-C in the South of Madagascar may have promoted the infections of children by EV-C, including the PV vaccine strains, and have favored the recombination events between PVs and NPEVs in co-infected children, thus leading to the recurrent emergence of recombinant VDPVs in this region of Madagascar.

Combining conventional tree-ring measurements with wood anatomy and strontium isotope analyses enables dendroprovenancing at the local scale

Dendroprovenancing provides critical information regarding the origin of wood, allowing further insights into economic exploitation strategies and source regions of timber products. Traditionally, dendroprovenancing relies on pattern-matching of tree rings, but its spatial resolution is limited by the geographical coverage of species-specific chronologies available for crossdating and, in the case of short-distance trades, by scarce environmental variability. Here, we present an approach to provenance timber with high spatial resolution from forested areas that have been exploited intensively throughout history, with the aim to understand the sustainability of the various woodland management practices used to supply timber products. To this end, we combined tree-ring width (TRW), wood anatomical and geochemical analyses in addition to multivariate statistical validation procedures to trace the origin of living oak trees (Quercus robur) sampled in four stands located within a 30-km radius around the city of Limoges (Haute-Vienne, France). We demonstrate that TRW and wood anatomical variables (and in particular cell density) robustly discriminate the eastern from the western site, while failing to trace the origin of trees from the northern and southern sites. Here, strontium isotopic ratios (87Sr/86Sr) and Ca concentrations identify clusters of trees which could not be identified with TRW or wood anatomy. Ultimately, our study demonstrates that the coupling of wood anatomy with geochemical signatures allows to correctly pinpoint the origin of trees. Given the small geographic scale of our study and the limited differences in elevation and climate between study sites, our results are particularly promising for future dendroprovenancing studies. We thus conclude that the combination of multiple approaches will not only increase the accuracy of dendroprovenancing studies at local scales, but could also be implemented at much larger scales to identify trends in historic timber supply throughout Europe.

We belong to the land: review of two northern rewilding sites as a vehicle for equity in conservation

The current planetary shifts and the redistribution of species require us to rethink nature conservation, both from the viewpoint of forming novel ecosystems and in relation to issues of diversity, historical conservation practices, as well as historical and current injustice. Mainly, this reorientation needs to address the historical errors of mainstream societies that promoted nature conservation for their own benefit, at the expense of Indigenous societies. The article explores two Finnish and Sámi rewilding cases of community-led action. In the end, the research seeks to answer how to negotiate a new co-existence with rewilded sites to achieve a better future.

Marine heatwaves in shallow coastal ecosystems are coupled with the atmosphere: Insights from half a century of daily in situ temperature records

Marine heatwaves (MHWs) are extreme ocean temperature events that can have wide-ranging and pervasive effects on marine species and ecosystems. However, studies of MHW characteristics and drivers primarily focus on open-ocean environments, rather than the nearshore coastal ocean (<10 km from coast, <50 m depth). This is despite coastal waters sustaining significant commercial, recreational, and customary fisheries and aquaculture activities that are highly susceptible to the impacts of MHWs. The two longest (>50 year) daily in situ ocean temperature records in the Southern Hemisphere are used to investigate the variability, drivers, and trends of MHWs in shallow water marine ecosystems (SWMEs). Located at the northern and southern limits of New Zealand, both locations experience an average of two to three MHWs annually, with MHWs at the exposed coastline site generally being of longer duration but less intense than those observed within the semi-enclosed harbor site. Observed MHWs have timescales similar to synoptic weather systems (9–13 days) and are most intense during Austral summer with little seasonality in frequency or duration. An investigation of MHWs co-occurring in nearshore coastal and offshore waters suggests that MHWs in semi-enclosed waters (e.g., harbors, estuaries) are more closely coupled with local atmospheric conditions and less likely to have a co-occurring offshore MHW than those occurring on exposed coastlines. Composite analysis using a reanalysis product elucidates specific atmospheric drivers and suggests that atmospheric pressure systems, wind speed and latent heat fluxes are important contributing factors to the generation and decline of MHWs in SWMEs. Investigation of long-term trends in MHW properties revealed an increase in MHW duration and annual MHW days at the southern site and decrease in maximum intensity at the northern site. This is consistent with broad-scale warming trends previously documented at these coastal stations, with differences related to changes in large-scale circulation patterns around New Zealand. Our results highlight the importance of in situ data for the analysis of MHW events in the nearshore coastal ocean, and the role of local atmospheric forcing in modulating the occurrence of MHWs in SWMEs, which can cause decoupling of temperature dynamics with the surrounding shelf sea.

Spatial and temporal variation of aseismic creep along the dilational jog of the West Valley Fault, Philippines: Hazard implications

Accelerated creep, primarily through vertical displacement, has been occurring along 15 en-echelon faults belonging to a 15 km-long and 1.5 km-wide, N-S-trending dilational jog of the active West Valley Fault (or West Marikina Valley Fault), on the southeastern part of Metro Manila, Philippines. The much-larger-than-known tectonic slip rates had been the only reliable evidence in support of excessive groundwater withdrawal as the trigger of creep in the 1990s. Recently available groundwater extraction data (1977–2019) could more directly and consistently link groundwater withdrawal to accelerated creep in the 1990s. Twenty years (1999–2019) of precise displacement measurements could also reveal significant spatial and temporal links between slip rate changes and patterns of groundwater extraction. Our analysis shows that greater rates of vertical displacement are related to the timing of faster extraction rates. Variations in slip rates between the northern and southern measurement sites are primarily due to regional differences in groundwater extraction, which are influenced by differences in the implementation of water extraction reduction regulations. Proximity to the key source of groundwater recharge (Laguna Lake) is also an influencing factor. Although there are many unknowns inherent to this type of study, continued depressurization could induce static stress changes that could modulate the timescale of earthquake occurrence due to the natural course of stress loading driven by regional tectonics. The current and potential effects of continued depressurization in the areas with high slip rates and surrounding regions are paramount considerations in crafting and implementing tighter and extended groundwater extraction regulations.

Pollution characteristics of typical ARGs in the sediments of the sea area adjacent to the Yangtze Estuary, China

The pollution characteristics of the typical antibiotic resistance genes (ARGs) in the sediments of the sea area adjacent to the Yangtze Estuary, China were investigated with both seasonal and spatial insights. The positive rates of sulfonamides sul1, sul2, tetracycline tetW and quinolone gyrA resistance genes in the sediments of all sampling sites were 100%, indicating that the sea area adjacent to the Yangtze Estuary were extensively polluted by these ARGs. Occupying a dominance in most sampling sites, sul1 was found to be the dominant resistance gene in the sediments of the sea area adjacent to the Yangtze Estuary. In terms of seasonal variation characteristics, the absolute abundances of the ARGs in flood season were higher than those in dry season. In terms of spatial distribution characteristics, the absolute abundances of the ARGs in the southern sites were mostly higher than those in the northern sites, and the high abundance sites were mostly located near the coast. Different ARGs also showed different spatial distribution characteristics. The disclosure of the pollution characteristics of the typical ARGs in the sediments of the sea area adjacent to the Yangtze Estuary can provide valued information for protecting the environment of this area as well as the Yangtze River Basin.

Integrating ZooMS and zooarchaeology: New data from the Uluzzian levels of Uluzzo C Rock Shelter, Roccia San Sebastiano cave and Riparo del Broion.

In this study we explore the potential of combining traditional zooarchaeological determination and proteomic identification of morphologically non-diagnostic bone fragments (ZooMS) collected from the Uluzzian levels of three Italian sites: Uluzzo C Rock Shelter, Roccia San Sebastiano cave, and Riparo del Broion. Moreover, we obtained glutamine deamidation ratios for all the contexts analysed during routine ZooMS screening of faunal samples, giving information on collagen preservation. We designed a selection protocol that maximizes the efficiency of the proteomics analyses by excluding particularly compromised fragments (e.g. from taphonomic processes), and that aims to identify new human fragments by favouring bones showing morphological traits more similar to Homo. ZooMS consistently provided taxonomic information in agreement with the faunal spectra outlined by traditional zooarchaeology. Our approach allows us to delineate and appreciate differences between the analysed contexts, particularly between the northern and southern sites, related to faunal, environmental, and climate composition, although no human remains were identified. We reconstructed the faunal assemblage of the different sites, giving voice to morphologically undiagnostic bone fragments. Thus, the combination of these analyses provides a more complete picture of the faunal assemblage and of the paleoenvironment during the Middle-Upper Palaeolithic transition in Italy.

Evaluating the Impact of Nutrient Doses on Biostimulation of Petroleum Hydrocarbon Biodegradation in Cold Region Soils

Biostimulation by addition of nitrogen (N) and phosphorus (P) for enhancement of biodegradation of petroleum hydrocarbons in contaminated soils is a common practice in sub-Arctic (cold) regions. Based on the data reported in 58 peer-reviewed papers on hydrocarbon degradation in northern region soils, there was no identifiable optimal nutrient dose, although applied doses ranged over 3 orders of magnitude. Microcosm slurry biodegradation experiments conducted over a range of N (41 to 1350 mg/kg) and P (46 and 115 mg/kg) doses, using a northern site soil spiked with Arctic diesel, also showed comparable results. While addition of nutrients improved degradation extents, the degradation extents were not dependent on N and P doses. Biodegradation rate constants for C10–C16 and C16–C24 hydrocarbon fractions, however, showed the highest enhancements for the lowest N dose. Microbial community composition analysis based on 16S rRNA sequencing of DNA extracted from microcosms amended with only diesel, only nutrients, and both diesel and nutrients revealed that diesel enriched hydrocarbon degraders such as Pseudomonadaceae and Burkholderiaceae. Overall, our results and analyses show limited benefits of biostimulation of hydrocarbon degradation with high nutrient doses, and low nutrient doses are generally more or equally effective.

Phylogenetic diversity of eastern Asia–eastern North America disjunct plants is mainly associated with divergence time

The underlying causes of biodiversity disparities among geographic regions have long been a fundamental theme in ecology and evolution. However, the patterns of phylogenetic diversity (PD) and phylogenetic beta diversity (PBD) of congeners that are disjunctly distributed between eastern Asia–eastern North America (EA−ENA disjuncts) and their associated factors remain unknown. Here we investigated the standardized effect size of PD (SES-PD), PBD, and potentially associated factors in 11 natural mixed forest sites (five in EA and six in ENA) where abundant EA−ENA disjuncts occur. We found that the disjuncts in ENA possessed higher SES-PD than those in EA at the continental scale (1.96 vs −1.12), even though the number of disjunct species in ENA is much lower than in EA (128 vs 263). SES-PD of the EA−ENA disjuncts tended to decrease with increasing latitude in 11 sites. The latitudinal diversity gradient of SES-PD was stronger in EA sites than in ENA sites. Based on the unweighted unique fraction metric (UniFrac) distance and the phylogenetic community dissimilarity, PBD showed that the two northern sites in EA were more similar to the six-site ENA group than to the remaining southern EA sites. Based on the standardized effect size of mean pairwise distances (SES-MPD), nine of eleven studied sites showed a neutral community structure (−1.96 ≤ SES-MPD ≤ 1.96). Both Pearson's r and structural equation modeling suggested that SES-PD of the EA–ENA disjuncts was mostly associated with mean divergence time. Moreover, SES-PD of the EA–ENA disjuncts was positively correlated with temperature-related climatic factors, although negatively correlated with mean diversification rate and community structure. By applying approaches from phylogenetics and community ecology, our work sheds light on historical patterns of the EA−ENA disjunction and paves the way for further research.

Algal Density Controls the Spatial Variations in Hg Bioconcentration and Bioaccumulation at the Base of the Pelagic Food Web of Lake Taihu, China.

Algal density can significantly impact mercury (Hg) bioaccumulation and biomagnification in aquatic food webs, but the underlying mechanisms remain controversial especially in subtropical and tropical regions. We conducted a comprehensive field study on Hg bioconcentration in phytoplankton and bioaccumulation in size-fractionated zooplankton across 17 sampling sites in Lake Taihu, a large shallow lake in eastern China with large spatial differences in algal density. The higher algal density in the northern sites is highly associated with the lower THg bioconcentration factor (BCF) in phytoplankton and lower THg bioaccumulation factor (BAF) in zooplankton. The low Hg BCFs or BAFs at productive sites could not be explained by algal bloom dilution but attributed to the low Hg bioavailability, which is highly associated with the elevated pH levels at productive sites. The smaller body size of the dominant zooplankton species at higher algal density sites also contributed to their lower Hg bioaccumulation. Importantly, we provide evidence that high algal density is associated with a low proportion of methylmercury (MeHg) in total Hg (% MeHg) in phytoplankton, which is further transferred to zooplankton. Such a low THg BCF or BAF and low % MeHg in plankton at high algal density sites hamper the entry of Hg into the pelagic food webs, which are important but yet underestimated driving forces for the low Hg contents in pelagic fish that are commonly observed in anthropogenic-impacted eutrophic lakes in subtropical regions.

Nitrogen and sulfur compounds in ambient air and in wet atmospheric deposition at Mexico city metropolitan area

Mexico City Metropolitan Area (MCMA), one of the most populous cities in the world, has historically represented a challenge in terms of air pollution. Particulates and sulfur dioxide.(SO2) were identified as the main atmospheric pollutants three decades ago. In order to reduce emissions of these pollutants, replacing of fuel oil by natural gas in power plants was carried out in the late 1980s. This strategy resulted in the reduction of ambient air concentrations of both pollutants. SO2 no longer exceeds its ambient air quality standard; however, acid rain remains a significant issue, with sulfate remaining as the principal component in the wet atmospheric deposition.In this study, spatial and temporal variations in the chemical composition of precipitation (sulfate and nitrate ions, and pH) were obtained weekly at 16 sampling stations, as well as atmospheric SO2 and nitrogen oxides (NOx) from an air quality monitoring network from 2003 to 2018 in the MCMA. Some indicators were applied to evaluate wet atmospheric deposition: SO42−/NO3− and NH4+/NO3− ratios, fractional acidity as well as neutralization factors.SO2 ambient air concentrations have decreased from 2003 to 2018 by more than 70%, while NOx has decreased by 20%. The SO2/NOx ratio has declined about 60%, indicating more effective strategies in reducing SO2 than for NOx emissions. The NOx emission sources are more diverse than for SO2, and NOx has more complex mechanisms of atmospheric deposition and photochemistry.The pH values of the samples at the stations located in the south were more acidic than the samples for the stations in the north. This result is in line with meteorological conditions, where prevailing winds blow from north to south, as well as emission sources located in the north sector, both inside and outside MCMA. For SO42−/NO3− ratio, all sampling sites present current values in the same magnitude order, around 1.4, which is higher compared with urban sites in USA. The 2018 NH4+/NO3− ratio was 1.98 for the southern sampling site, while all northern sampling sites were approximately 2.28. These results are on the same order that urban sites in USA. It was concluded that ammonium was a very important neutralizing agent of pH within the MCMA precipitation.It is recommended to apply strategies for the emissions reduction of acid rain precursors in external sources to the North the MCMA, and to establish a Network for Urban Atmospheric Nitrogen Chemistry.

Extrinsic and intrinsic drivers of parasite prevalence and parasite species richness in a marine bivalve.

Parasite species richness is influenced by a range of drivers including host related factors (e.g. host size) and environmental factors (e.g. seawater temperature). However, identification of modulators of parasite species richness remains one of the great unanswered questions in ecology. The common cockle Cerastoderma edule is renowned for its diversity and abundance of parasites, yet drivers of parasite species richness in cockles have not been examined to investigate the association of both macro and microparasite communities. Using cockles as a model species, some of the key drivers of parasite prevalence and parasite species richness were investigated. Objectives of this 19-month survey were to determine the influence of the environment, host-parasite dynamics and parasite associations on parasite species richness and prevalence at two different geographic latitudes, chosen based on environmental differences. The highest parasite species richness was recorded in the northern sites, and this was potentially influenced by a range of interactions between the host, the pathogens and the environment. Parasite prevalence increased with host size and age, and parasite species richness increased with reduced salinity. A number of interactions between parasites, and between parasites and pathologies may be influencing parasite infection dynamics. New and concerning information is also presented regarding interactions between parasites and their environment. A number of parasites and potential pathogens (bacteria, Trichodina ciliates, metacercariae, trematode sporocysts) may be advantaged under climate change conditions (warming seas, increased precipitation), increasing disease incidence, which may prove detrimental not just for cockles, but for other bivalve species in the future.

Quantifying phenology and migratory behaviours of hummingbirds using single-site dynamics and mark-detection analyses.

Nuanced understanding of seasonal movements of partially migratory birds is paramount to species and habitat conservation. Using nascent statistical methods, we identified migratory strategies of birds outfitted with radio-frequency identification (RFID) tags detected at RFID feeders in two sites in California, USA. We quantified proportions of migrants and residents and the seasonal phenology for each movement strategy in Allen’s and Anna’s hummingbirds; we also validated our methodology by fitting our model to obligate migratory black-chinned hummingbirds. Allen’s and Anna’s hummingbirds exhibited characteristics of facultative migratory behaviour. We also quantified apparent annual survival for each migratory strategy and found that residents had significantly higher probabilities of apparent survival. Low survival estimates for migrants suggest that a high proportion of birds in the migrant group permanently emigrated from our study sites. Considered together, our analyses suggest that hummingbirds in both northern and southern California sites partake in diverse and highly plastic migratory behaviours. Our assessment elucidates the dynamics underlying idiosyncratic migratory behaviours of two species of hummingbirds, in addition to describing a framework for similar assessments of migratory behaviours using the multi-state open robust design with state uncertainty model and single-site dynamics.

Temporal Variation Analysis of Rice Yield in the Jiangsu Province, China: Application of Decision Support System for Agrotechnology Transfer Model

The accuracy of grain yield estimation is critical for national food security. Because of the comprehensive influence of spatial differentiation conditions, such as temperature, precipitation, soil, rice variety, and irrigation, yield estimation requires integrated modeling that is based on dynamic conditions. These dynamic conditions include geographical background, biological factors, and human impact. Most existing studies focus on the observation and analysis of external factors; only a few reports on yield simulations are coupled with nature, management, and crop growth mechanism. Our study incorporates the crop growth mechanism of rice, along with data of rice varieties, soil, meteorology, and field management, to determine the rice yield in Jiangsu province, China. In addition, we have used a decision support system for the agrotechnology transfer model, along with Coupled Model Intercomparison Project data and geographic information system technology. Our results showed that: (1) A calibrated variety genetic coefficient could simulate rice biomass value (flowering stage, maturity stage, and yield) reasonably. The values of NRMSE (Normalized Root Mean Square Error) between the simulated and measured values after parameter calibration are all less than 10%, the values of d(index of agreement) are all close to 1, the simulated value of yield is in good agreement with the measured value. (2) A linear correlation between the meteorological elements and yield was observed. The linear correlation had regional differences. Notably, an increase in precipitation was conducive to the increase in yield. Except at the Huaiyin site, the other sites showed that the temperature rise could potentially lead to reduced production. We found that an increase in solar radiation was unfavorable to the production of rice in the northern and western sites in the Jiangsu province, whereas it was conducive in the southern and eastern sites. (3) Our study predicted the rice yield from typical sites in the Jiangsu province from 2019 to 2060 in the wake of climate change while excluding the extreme effects of diseases, pests, typhoons, and floods. The order of average yield per unit area is as follows: Xinghua site (8212.76 kg/ha) > Huaiyin site (7912.70 kg/ha) > Gaoyou site (7440.98 kg/ha) > Gaochun site (7512.29 kg/ha) > Ganyu site (7460.88 kg/ha) > Yixing site (7167.00 kg/ha). Notably, the average yields from the Xinghua and Huaiyin sites were higher than that from the Jiangsu province (7617.77 kg/ha). The fluctuation of the yield per unit area at each site was generally consistent with the fluctuation in the overall yield, showing a downward trend and tends to be stable. The dispersion of yield per unit area indicates that Gaochun had the most stable yield per unit area followed by Xinghua, Ganyu, Yixing, Huaiyin, and Gaoyou. The yield per unit area of the Huaiyin and Gaoyou sites was unstable and portrayed the biggest fluctuations. Future studies need to focus on how to deal with spatial variation and carry out adaptive verification to make the simulation results applicable to more dimensions.

Controls on organic matter variation during deposition of the Mahogany Oil Shale Zone of the Parachute Creek Member, Green River Formation, Utah

The Green River Formation of Utah, Colorado, and Wyoming represents a ~10 million-year early-middle Eocene record of an unusually large, productive lacustrine system composed of several interconnected basins. This system gave rise to one of the largest oil shale deposits in the world, which preserves a rich trove of information about the climate and ecosystems that prevailed during the Early Eocene Climatic Optimum. This study uses multiple analytical approaches, including both traditional methods and novel proxies, to determine what brought about the accumulation of the unusually-rich oil shales (>40% TOC) of the Mahogany Zone in the Uinta Basin portion of the Green River Formation. The organic-rich mudstones of the Mahogany Zone exhibit strong micro-scale heterogeneity, indicating complex controls on organic matter production and distribution. Petrographic observations indicate highly variable amounts of terrestrial organic matter (spores, wood and plant debris), with the most abundant being amorphous organic material in thin organic-rich laminae. Biomarker ratios indicate that the organic-rich laminae consist of different types of microbially derived organic matter, primarily bituminite and organo-minerallic aggregates of the fluorescent liptinite group. These largely lacustrine-derived laminated deposits are argued to have been produced by large intrato inter-annual algal blooms and other enhanced microbial productivity events paced by longer-term sub-orbital cycle fluctuations, such as El Niño–Southern Oscillation or sunspot variations. The Mahogany Zone deposits appear to have sequestered enough carbon (~64.25 Gt estimated) to suggest that, in aggregate, they were large enough to cause a draw-down of terrestrial CO2 and exert a significant negative feedback effect on climatic warming. During the climatic cooling that occurred on the declining limb of the Early Eocene Climatic Optimum, the sedimentary provenance in the Uinta Basin shifted from southerly sources dominated by large ephemeral fluvial systems, to northerly sources rich in feldspathic and carbonate detritus derived from northern sites in Wyoming. The latter were delivered to the Uinta Basin via a network of interconnected lacustrine basins. Increasing volcaniclastic material delivered from these northerly basins infilled the Uinta Basin from east to west, and their arrival heralded the end of organic-rich deposition, most notably the prolific oil shales for which the Green River Formation is renowned.

Absolute Paleolatitude of Northern Zealandia From the Middle Eocene to the Early Miocene

AbstractThe absolute position during the Cenozoic of northern Zealandia, a continent that lies more than 90% submerged in the southwest Pacific Ocean, is inferred from global plate motion models, because local paleomagnetic constraints are virtually absent. We present new paleolatitude constraints using paleomagnetic data from International Ocean Discovery Program Site U1507 on northern Zealandia and Site U1511 drilled in the adjacent Tasman Sea Basin. After correcting for inclination shallowing, five paleolatitude estimates provide a trajectory of northern Zealandia past position from the middle Eocene to the early Miocene, spanning geomagnetic polarity chrons C21n to C5Er (∼48–18 Ma). The paleolatitude estimates support previous works on global absolute plate motion where northern Zealandia migrated 6° northward between the early Oligocene and early Miocene, but with lower absolute paleolatitudes, particularly in the Bartonian and Priabonian (C18n–C13r). True polar wander (solid Earth rotation with respect to the spin axis), which only can be resolved using paleomagnetic data, may explain the discrepancy. This new paleomagnetic information anchors past latitudes of Zealandia to Earth's spin axis, with implications not only for global geodynamics, but also for addressing paleoceanographic and paleoclimate problems, which generally require precise paleolatitude placement of proxy data.

Community composition of coral-associated Symbiodiniaceae differs across fine-scale environmental gradients in Kāne'ohe Bay.

The survival of most reef-building corals is dependent upon a symbiosis between the coral and the community of Symbiodiniaceae. Montipora capitata, one of the main reef-building coral species in Hawai'i, is known to host a diversity of symbionts, but it remains unclear how they change spatially and whether environmental factors drive those changes. Here, we surveyed the Symbiodiniaceae community in 600 M. capitata colonies from 30 sites across Kāne'ohe Bay and tested for host specificity and environmental gradients driving spatial patterns of algal symbiont distribution. We found that the Symbiodiniaceae community differed markedly across sites, with M. capitata in the most open-ocean (northern) site hosting few or none of the genus Durusdinium, whereas individuals at other sites had a mix of Durusdinium and Cladocopium. Our study shows that the algal symbiont community composition responds to fine-scale differences in environmental gradients; depth and temperature variability were the most significant predictor of Symbiodiniaceae community, although environmental factors measured in the study explained only about 20% of observed variation. Identifying and mapping Symbiodiniaceae community distribution at multiple scales is an important step in advancing our understanding of algal symbiont diversity, distribution and evolution and the potential responses of corals to future environmental change.

Translocating seed sources to new geoclimatic environments has limited effect on lumber quality of eastern Canadian white spruce

Assisted gene flow according to expected climate gradients is considered as a forest management strategy to mitigate impacts of environmental change on forest growth. However, the effects of seed translocation on wood properties and lumber quality remain unknown. This study evaluated the effect of provenance origin on lumber production and quality at rotation age in two white spruce provenance trials established in contrasting environments in eastern Canada. Based on 108 sample trees, which resulted in 943 pieces of lumber, average volume production per tree at the southernmost site was twice that of the production at the northern site. Provenance had a significant influence on growth and lumber strength in the first sawlog but had no effect on lumber stiffness and wood density. Although visual grade yields of No. 2 and better were high in both trials (over 86%), the machine stress rated (MSR) grade potential and percentage of lumber that met the bending stiffness design values of the visual grades were generally low (12%–26%). Hence, plantation-grown lumber should preferably be machine stress rated to ensure its fitness for structural applications in buildings. Management strategies aiming to efficiently sequester carbon should primarily maximize volume productivity in northern sites, as moving seed sources north still reduces provenance productivity, while breeding programs should aim to prevent decrease in lumber stiffness due to augmented productivity and shortened rotation cycles.

Network Structure of Bat-Ectoparasitic Interactions in Tropical Dry Forests at Two Different Regions in Brazil

Network analysis has been used for understanding complex systems in biology for decades. However, scant information is available for networks of antagonistic interactions. The aim of this study was to describe and compare bat-ectoparasite interaction networks in tropical dry forests (TFDs) in the Brazilian Cerrado and Caatinga biomes, which have contrasting environmental conditions and surrounding matrix. Bats and ectoparasites were sampled at six sites in northern and central Minas Gerais State, southeast Brazil. Network connectance, nestedness and number of compartments were compared between regions, as well as bat species richness, abundance and centrality, and ectoparasite specialization. The effect of bat phylogenetic relatedness on the similarity of their associated ectoparasite species was also tested. Bat-ectoparasite networks were nested, connected and highly compartmentalized in TDFs from both regions, with no significant differences in network structure. In addition, host species richness negatively influenced nestedness and connectance, but was positively related to compartment number. These findings are likely related to the high specialization observed for bat-fly species (usually one exclusive parasite species per host), resulting in networks with few interactions. Bat abundance positively affected bat-fly richness, indicating that resource availability is important in determining host-parasite relationships. Finally, phylogenetically related species of bats possessed more similar ectoparasite faunas, which may be associated with evolutionary responses of ectoparasites to escape defensive mechanisms of different bat species. Our results suggest that local bat-ectoparasite interactions are influenced by both ecological factors and evolutionary constraints, but the effects of environmental conditions on network topology deserved further detailed studies.

Can delaying germination reduce barriers to successful emergence for early‐germinating, fall‐sown native bunchgrass seeds in cold deserts?

Efforts to restore semiarid wildlands in the western United States predominantly use fall seeding. Fall conditions are more amenable to seeding, and successfully over‐wintered seeds or plants are poised to take full advantage of spring moisture. However, over‐winter mortality can be a barrier to seeding success. One solution to avoid winter mortality without sacrificing the benefits of fall seeding is to delay germination of fall‐sown seeds. At six northern Great Basin field sites over three planting years (18 trials), we tested whether a hydrophobic seed coating reliably delayed germination and increased seedling establishment of a native bunchgrass. Despite considerable variation among sites and years, seed treatment successfully reduced pre‐winter germination by 84% compared to untreated, but also consistently reduced final germination by 23%. Still, treatment resulted in an average of 2.1‐fold higher emerged seedling density in seven trials, six of which were among the eight trials where more than 50% of untreated seeds germinated pre‐winter. Emergence was greater for untreated seed in five trials, all of them in the same year, and all but one of them with below‐average total germination. Our treatment consistently reduced pre‐winter germination, but only improved emergence when pre‐winter germination of untreated seeds was high. Continued research is merited with germination‐delaying treatments and to experimentally define—and develop models that predict—winter‐related mortality barriers. We also suggest future exploration of bet‐hedging strategies that mix treated and untreated seeds where fall seeding is required and significant but variable risk of over‐winter seedling mortality exists.