Northern Regional Research Laboratory Research Articles

Streptomyces venezuelae NRRL B-65442: genome sequence of a model strain used to study morphological differentiation in filamentous actinobacteria.

For over a decade, Streptomyces venezuelae has been used to study the molecular mechanisms that control morphological development in streptomycetes and is now a well-established model strain. Its rapid growth and ability to sporulate in a near-synchronised manner in liquid culture, unusual among streptomycetes, greatly facilitates the application of modern molecular techniques such as ChIP-seq and RNA-seq, as well as time-lapse fluorescence imaging of the complete Streptomyces life cycle. Here we describe a high-quality genome sequence of our isolate of the strain (Northern Regional Research Laboratory [NRRL] B-65442) consisting of an 8.2 Mb chromosome and a 158 kb plasmid, pSVJI1, which had not been reported previously. Surprisingly, while NRRL B-65442 yields green spores on MYM agar, the American Type Culture Collection (ATCC) type strain 10712 (from which NRRL B-65442 was derived) produces grey spores. While comparison of the genome sequences of the two isolates revealed almost total identity, it did reveal a single nucleotide substitution in a gene, vnz_33525, involved in spore pigment biosynthesis. Replacement of the vnz_33525 allele of ATCC 10712 with that of NRRL B-65442 resulted in green spores, explaining the discrepancy in spore pigmentation. We also applied CRISPR-Cas9 to delete the essential parB of pSVJI1 to cure the plasmid from the strain without obvious phenotypic consequences.

Phylogenomic analysis of the Brevibacillus brevis clade: a proposal for three new Brevibacillus species, Brevibacillus fortis sp. nov., Brevibacillus porteri sp. nov. and Brevibacillus schisleri sp. nov.

During a screen for antifungal activity of Brevibacillus strains in the Northern Regional Research Laboratory collection we identified two strains with strong activity. Subsequent genomic sequencing and phylogenomic analysis revealed that these strains (NRRL NRS-1210T and NRRL B-41110T) are likely novel species. To confirm their taxonomic placement, we conducted a 16S rRNA phylogenetic analysis and subsequently sequenced the genomes of 10 Brevibacillus type strains with a 16S homology > 97%. Phylogenomic analysis of these type strains and of representative Brevibacillus strains deposited in GenBank also identified several novel clades that should be recognised as novel species. For one of these novel clades, we were able to obtain a publicly available isolate (ATCC 35690T) that could serve as a type strain. The three new species were subjected to a polyphasic characterisation to confirm their taxonomic status. Cells of strains NRRL NRS-1210T, NRRL B-41110T and ATCC 35690T are Gram-staining positive, motile and form tan colonies. All three strains are obligate aerobic mesophiles with a broad pH range for growth. The two most prominent fatty acids of the three strains were identified as iso-C15:0 and anteiso-C15:0. The DNA G+C contents of strains NRRL NRS-1210T, NRRL B-41110T and ATCC 35690T are 47.2mol%, 47.1mol% and 47.3mol%, respectively. Based on these characteristics, three novel species are proposed: Brevibacillus fortis sp. nov. (NRRL NRS-1210T = DSM 9886T = ATCC 51666T), Brevibacillus porteri sp. nov. (NRRL B-41110T = KACC 19693T) and Brevibacillus schisleri sp. nov. (ATCC 35690T = LMG 17055T).

Pediocin SA-1: A selective bacteriocin for controlling Listeria monocytogenes in maize silages.

In this study, we assessed the potential as silage additive of a bacteriocin produced by Pediococcus acidilactici Northern Regional Research Laboratory (NRRL) B-5627 (pediocin SA-1). Maize was inoculated either with a bacterial starter alone (I) or in combination with the bacteriocin (IP), and untreated silage served as control. We monitored the products of fermentation (ethanol, and lactic and acetic acids), the microbial population, and the presence of the indicator strain Listeria monocytogenes Colección Española de Cultivos Tipo (CECT) 4032 (1×10(5) cfu/g) after 1, 2, 5, 8, 16, and 30d of ensiling. Our results indicated antilisterial activity of the bacteriocin, anticipating the disappearance of L. monocytogenes in IP compared with I and control silages. The PCR-denaturing gradient gel electrophoresis analysis revealed the addition of the bacteriocin did not affect the bacterial communities of the spontaneous fermentation, and the inoculant-containing bacteria (Lactobacillus plantarum, Lactobacillus buchneri, and Enterococcus faecium) were found in addition to the bacterial communities of untreated maize silages in I and IP silages. Both treatments increased the concentration of antimicrobial compounds (acetic acid, ethanol, and 1,2-propanodiol) and led to lower residual sugar contents compared with the control, which would provide enhanced aerobic stability. The fact that the identified species L. plantarum, L. buchneri, and E. faecium produce some of these inhibitory compounds, together with their persistence throughout the 30d of fermentation, suggest these bacteria could actively participate in the ensiling process. According to these results, pediocin SA-1 could be used as an additive to control the presence of L. monocytogenes in maize silages selectively, while improving their fermentative quality and eventually their aerobic stability.

Science and Secrecy

On 17 September 1945 Robert Coghill, fermentation chief at the US Department of Agriculture's Northern Regional Research Laboratory, Peoria, Ill., wrote a letter to Otto Behens of Eli Lilly and Company. In it he said he was “very sorry that we shall not be able to cooperate with you as closely in the future as we have in the past, because we have discontinued our work on penicillin at this laboratory … the whole problem has lost any semblance of urgency.”

Historical aspects of antitumor compounds from plants, including homoharringtonine (Omacetaxine Mepesuccinate, SynriboTM)

Various preparations derived from vascular plants have been used utilized to treat tumors or cancerous conditions for centuries. The available literature was summarized by Jonathan Hartwell in a remarkable series of papers “Plants Used Against Cancer” published in Lloydia from 1967 to 1971. The U.S. National Cancer Institute (NCI) established the Cancer Chemotherapy National Service Center (CCNSC) in 1955. Hartwell transferred to CCNSC in 1957, and under his guidance a major program to discover plant anticancer constituents was developed. Arrangements were made between the NCI and the U.S. Department of Agriculture (USDA) for extensive plant collections in 1960. Many of the collections were from countries that were involved in the Food for Peace Program, Public Law (PL) 480. The Wisconsin Alumni Research Foundation (WARF) was awarded an extraction and screening contract, and research contracts were awarded to Drs. Monroe Wall, Morris Kupchan, Norman Farnsworth, John Cassady and others. When plant seeds were obtained, they were sent to the Northern Regional Research Laboratory (NRRL) in Peoria, Illinois, where they were examined in a program to identify potential new crops. Extracts of many of the seeds were prepared and screened for antitumor activity and the more significant results obtained from the seed extracts will be summarized.

Xylanase Production by Mixed Culture Using Crude Hemicellulose from Rice Straw Black Liquor and Peat Moss as an Inert Support

Black liquor (BL) is a by-product of rice straw pulping process. It is a low costs raw material for production value-adding proteins and enzymes, which has been paid more and more attention to reduce its environmental pollution. Mixed cultures of micelial fungi, Trichoderma reesei Northern Regional Research Laboratory (NRRL)11236, Trichoderma reesei NRRL 6165 and Aspergillus niger strains NRC 5A, NRC 7A, and NRC 9A were evaluated for their ability to produce xylanase using crude hemicellulose (CHC) prepared from BL and peat moss as an inert support under solid state fermentation (SSF). The most potent strains, A. niger NRC 9A (818.26 U/g CHC) and T. reesei NRRL 6165 (100.9±57.14 U/g CHC), were used in a mixed culture to enhance xylanase production by co-culturing under SSF. In the mixed culture, xylanase production (1070.52±12.57 U/g CHC) was nearly1.3 and 10.6-fold increases over the activities attained in their monocultures, A. niger NRC 9A and T. reesei NRRL 6165, respectively. Optimization of the culture parameters of the mixed culture SSF process, concentration of ammonium sulfateand corn steep liquor, CHC/peat moss ratio, inoculum size and ratios of the two strains, initial pH value, initial moisture content and incubation time, exhibited a significant increase (2414.98± 84.02 U/g CHC) in xylanase production than before optimization.

In vitro Study of the Potential Protective Role of Lactobacillus Strains by Acrylamide Binding

AbstractAcrylamide is a chemical naturally formed in certain foods during processing and/or cooking at high temperatures. Scientific studies have indicated that significant dietary exposure to acrylamide poses a risk for several types of cancer and may have harmful effects to the nervous system in humans. Hence, methods that can contribute to reduce acrylamide exposure in foods are needed. Considering that specific lactic acid bacteria are able to remove a variety of foodborne mutagens, namely heterocyclic amines, lead, cadmium and aflatoxin B1 by a binding mechanism, the aim of the present study was to assess the ability of fourteen lactic acid strains to remove acrylamide in vitro. Acrylamide binding ability ranged from 11.89 to 29.12%, and it was found to be both concentration‐ and strain‐dependent. Lactobacillus reuteri Northern Regional Research Laboratory 14171 (USDA‐ARS) and Lactobacillus casei Shirota were the most efficient binders (24.01 and 24.95%, respectively) after 12 h of incubation using an acrylamide concentration of 5 μg/mL. Acrylamide binding was also pH‐dependent, and the bacterial–toxin complex exhibited high stability assessed by repeated washings. The results of this work proposes for the first time the acrylamide binding ability in vitro of selected lactic acid bacteria, which suggest their potential for detoxification of the food contaminant acrylamide.Practical ApplicationsAll bacteria tested proved capable to bind acrylamide efficiently and rapidly in phosphate buffer saline; hence, these bacteria could be an effective decontamination mechanism against foodborne acrylamide along the gastrointestinal tract. It is reasonable to believe that during the first contact of acrylamide‐contaminated food with lactic acid bacteria in the mouth, the place where digestion starts and the pH is favorable for the toxin–bacteria interaction, a portion of acrylamide may be bound by the bacteria. Moreover, as both toxin and bacteria navigate through the digestive tract, the variations in pH may cause mutagen binding at different extents until it reaches the small intestine (alkaline pH), the site where absorption of food components is the highest, and the pH value is favorable. Therefore, this kind of bacteria could play a protective role by reducing the bioavailability of foodborne acrylamide.

Paenibacillus larvae 16S–23S rDNA intergenic transcribed spacer (ITS) regions: DNA fingerprinting and characterization

Paenibacillus larvae is the causative agent of American foulbrood in honey bee (Apis mellifera) larvae. PCR amplification of the 16S–23S ribosomal DNA (rDNA) intergenic transcribed spacer (ITS) regions, and agarose gel electrophoresis of the amplified DNA, was performed using genomic DNA collected from 134 P. larvae strains isolated in Connecticut, six Northern Regional Research Laboratory stock strains, four strains isolated in Argentina, and one strain isolated in Chile. Following electrophoresis of amplified DNA, all isolates exhibited a common migratory profile (i.e., ITS-PCR fingerprint pattern) of six DNA bands. This profile represented a unique ITS-PCR DNA fingerprint that was useful as a fast, simple, and accurate procedure for identification of P. larvae. Digestion of ITS-PCR amplified DNA, using mung bean nuclease prior to electrophoresis, characterized only three of the six electrophoresis bands as homoduplex DNA and indicating three true ITS regions. These three ITS regions, DNA migratory band sizes of 915, 1010, and 1474bp, signify a minimum of three types of rrn operons within P. larvae. DNA sequence analysis of ITS region DNA, using P. larvae NRRL B-3553, identified the 3′ terminal nucleotides of the 16S rRNA gene, 5′ terminal nucleotides of the 23S rRNA gene, and the complete DNA sequences of the 5S rRNA, tRNAala, and tRNAile genes. Gene organization within the three rrn operon types was 16S–23S, 16S–tRNAala–23S, and l6S–5S–tRNAile–tRNAala–23S and these operons were named rrnA, rrnF, and rrnG, respectively. The 23S rRNA gene was shown by I-CeuI digestion and pulsed-field gel electrophoresis of genomic DNA to be present as seven copies. This was suggestive of seven rrn operon copies within the P. larvae genome. Investigation of the 16S–23S rDNA regions of this bacterium has aided the development of a diagnostic procedure and has helped genomic mapping investigations via characterization of the ITS regions.

Xanthan Biopolymer Semipilot Fermentation

Abstract Using standard microbiological techniques in a semipilot plant-scalable fermenter, xanthan-specific culture techniques developed by the U.S. Dept of Agriculture's Northern Regional Research Laboratory (NRRL) were used at Marathon Oil Co.'s Denver Research Center (DRC) to obtain improved and reproducible high conversions and yields of xanthan biopolymer broth. Practical nutrients and fermenter parameters were studied to define and improve the viscosity performance and economics of xanthan broth production for thickening water in the Maraflood™ enhanced oil recovery process.

Mycotoxic diseases produced in mice by species of the aspergillus ochraceus group

Twenty-three isolates (identified below by Northern Regional Research Laboratory culture numbers) comprising eight species of the Aspergillus ochraceus group were examined for toxicity in Swiss albino mice fed for 3 wk on diets containing rice cultures of the isolates mixed at 50% concentrations with a commercial purified diet. The histopathological changes produced and a capacity to produce ochratoxin A, penicillic acid or both mycotoxins were the criteria used to assign the isolates to one of five groups. Group I ( A. auricomus NRRL nos 387 and 388, A. petrakii 404, 416 and 4369, A. elegans 4850 and A. alliaceus 1237) included some strains that produced penicillic acid but none produced ochratoxin A and none caused a significant incidence of lesions or deaths. Group II isolates ( A. sclerotiorum 415, 1598 and 4901) did not produce ochratoxin A, but two produced penicillic acid and all caused a 100% death rate, principally with lesions of multifocal hepatic necrosis and fatty change. In group III ( A. alliaceus 315 and 4181), each isolate produced ochratoxin A and all the treated mice died. Renal lesions were consistent with those described for ochratoxicosis A. Group IV isolates ( A. sclerotiorum 5166 and 5170) produced both ochratoxin A and penicillic acid and killed over half of the treated mice. The main hepatic and renal lesions in these mice were necrotizing cholangitis, multifocal hepatic necrosis and degeneration and necrosis of the renal convoluted tubules. The group V isolates ( A. melleus 386 and 5103, A. ostianus 420 and 3524 and A. ochraceus 398, 399, 400, 402 and 410, as well as an isolate of Penicillium viridicatum, Purdue University Strain 66-68-2) did not produce ochratoxin A, but four produced penicillic acid and all caused hepatic and renal lesions identical to those observed with group IV isolates. The hepatic and renal changes observed in the mouse following treatment with either the group IV or group V isolates were similar to those described previously for an isolate of A. ochraceus and for several Indiana isolates of P. viridicatum.

Lethality in mice of double-stranded ribonucleic acid from virus-like particles of Penicillium stoloniferum: G. A. Sansing,E. B. Lillehoj,S. N. Freerand R. W. Detroy, Toxicon, 1974, 12, 513 (Northern Regional Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604, U.S.A.)

Lethality in mice of double-stranded ribonucleic acid from virus-like particles of Penicillium stoloniferum: G. A. Sansing,E. B. Lillehoj,S. N. Freerand R. W. Detroy, Toxicon, 1974, 12, 513 (Northern Regional Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604, U.S.A.)

Comments on “influence of shearing history on the properties of polymer melts. I”

Journal of Applied Polymer ScienceVolume 18, Issue 8 p. 2533-2534 NoteFree Access Comments on “influence of shearing history on the properties of polymer melts. I”† E. B. Bagley, E. B. Bagley Northern Regional Research Laboratory, Peoria, Illinois 61604Search for more papers by this author E. B. Bagley, E. B. Bagley Northern Regional Research Laboratory, Peoria, Illinois 61604Search for more papers by this author First published: August 1974 https://doi.org/10.1002/app.1974.070180833 † Z. Walczak J. Appl. Polym. Sci., 17, 153, 1973 AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat No abstract is available for this article. Volume18, Issue8August 1974Pages 2533-2534 ReferencesRelatedInformation

Bacillus alcalophilus subsp. halodurans subsp. nov.: An Alkaline-Amylase-Producing, Alkalophilic Organism

An alkaline-amylase-producing, alkalophilic bacillus, NRRL B-3881, was characterized and compared with Bacillus sp. ATCC 21591 and Bacillus alcalophilus Vedder strain NCTC 4553 (=ATCC 27647), which is here designated as the type strain of B. alcalophilus. All three strains contained motile, gram-positive rods with rounded ends and swollen, clavate sporangia with oval, terminal to subterminal endospores. All three strains grew in soybean broth; were facultatively anaerobic; hydrolyzed starch, gelatin, and casein; reduced methylene blue; and fermented the following carbohydrates without gas production: Sucrose, D-glucose, lactose, maltose, D-mannitol, D-xylose, L-arabinose, glycerol, sorbitol, and salicin. None produced acetylmethylcarbinol, indole, unrease, or crystalline dextrins. Bacillus sp. ATCC 21591 and Bacillus sp. NRRL B-3881, but not NCTC 4553, reduced nitrate to nitrite, utilized citrate, and grew well in 12% NaCl and slowly in 15% NaCl. B. alcalophilus NCTC 4553 did not grow in 5% NaCl. In our opinion, these differences are sufficient to justify the establishment of a separate subspecies for ATCC 21591, NRRL B-3881, and similar strains. We propose the name B. alcalophilus subsp. halodurans as the name for this new subspecies. The name of the type subspecies, which contains the type strain, NCTC 4553, is B. alcalophilus subsp. alcalophilus Vedder. NRRL B-3881 is designated as the type strain of B. alcalophilus subsp. halodurans and is available from the Northern Regional Research Laboratory. It has also been deposited in the American Type Culture Collection under the number 27557.

Recent research for the control of mycotoxins in cereal

Abstract

Glucose isomerase covalently bound to porous glass beads.

Biotechnology and BioengineeringVolume 14, Issue 3 p. 509-513 Communications to the EditorFree Access Glucose isomerase covalently bound to porous glass beads† G. W. Strandberg, G. W. Strandberg Northern Regional Research Laboratory, Northern Marketing and Nutrition Research Division, Agriculture Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604Search for more papers by this authorK. L. Smiley, K. L. Smiley Northern Regional Research Laboratory, Northern Marketing and Nutrition Research Division, Agriculture Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604Search for more papers by this author G. W. Strandberg, G. W. Strandberg Northern Regional Research Laboratory, Northern Marketing and Nutrition Research Division, Agriculture Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604Search for more papers by this authorK. L. Smiley, K. L. Smiley Northern Regional Research Laboratory, Northern Marketing and Nutrition Research Division, Agriculture Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604Search for more papers by this author First published: May 1972 https://doi.org/10.1002/bit.260140319Citations: 36 † Presented at 5th Great Lakes Regional American Chemical Society Meeting, Peoria, Illinois, June 10–11, 1971 AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume14, Issue3May 1972Pages 509-513 ReferencesRelatedInformation

Cost analyses for new products and processes developed in USDA laboratories

AbstractCost analyses frequently help to implement the research and development program on new products and processes from farm crops. Having cost data available encourages early adoption and application by industry of products and processes that are developed. Furthermore, cost estimates provide information that can serve as a guideline for developing an effective plan of work for research projects. The procedures used to compile cost estimates at the Northern Regional Research Laboratory follow those generally recommended by most cost engineers with appropriate modifications to fit particular situations. Accuracy of an estimate depends upon the quantity and quality of the process data made available to the cost engineer. Both order of magnitude estimates prepared shortly after studies on a process are initiated on a laboratory scale and preliminary cost estimates prepared either from extensive laboratory studies or from process engineering studies in the pilot plant have found application in research investigations. A typical example of such appications is found in our program for evaluating the industrial potential of several nylons which can be produced fromCrambe abyssinica oil.

WHEELS MAKE HIGH‐PRESSURE RESEARCH ROLL

Annals of the New York Academy of SciencesVolume 172, Issue 9 p. 155-174 WHEELS MAKE HIGH-PRESSURE RESEARCH ROLL John P. Friedrich Ph. D., John P. Friedrich Ph. D. Northern Regional Research Laboratory Peoria, Ill. A laboratory of the Northern Utilization Research and Development Division, Agricultural Research Service, U.S. Department of Agriculture. Mention of firm names or trade products does not imply endorsement or recommendation by the Department of Agriculture over other firms or similar products not mentioned.Search for more papers by this author John P. Friedrich Ph. D., John P. Friedrich Ph. D. Northern Regional Research Laboratory Peoria, Ill. A laboratory of the Northern Utilization Research and Development Division, Agricultural Research Service, U.S. Department of Agriculture. Mention of firm names or trade products does not imply endorsement or recommendation by the Department of Agriculture over other firms or similar products not mentioned.Search for more papers by this author First published: October 1970 https://doi.org/10.1111/j.1749-6632.1970.tb34974.xCitations: 2 AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume172, Issue9Third Confeence on Catalytic Hydrogenation and Analogous Pressure ReactionsOctober 1970Pages 155-174 RelatedInformation

Water‐Soluble Films from Partially Acetylated High‐Amylose Corn Starch

Starch - StärkeVolume 21, Issue 4 p. 92-96 Article Water-Soluble Films from Partially Acetylated High-Amylose Corn Starch A. M. Mark M. S., A. M. Mark M. S. Northern Regional Research Laboratory ,) Peoria, Illinois 61604 (USA)Search for more papers by this authorC. L. Mehltretter Ph. D., C. L. Mehltretter Ph. D. Northern Regional Research Laboratory ,) Peoria, Illinois 61604 (USA)Search for more papers by this author A. M. Mark M. S., A. M. Mark M. S. Northern Regional Research Laboratory ,) Peoria, Illinois 61604 (USA)Search for more papers by this authorC. L. Mehltretter Ph. D., C. L. Mehltretter Ph. D. Northern Regional Research Laboratory ,) Peoria, Illinois 61604 (USA)Search for more papers by this author First published: 1969 https://doi.org/10.1002/star.19690210403Citations: 6 This is a laboratory of the Northern Utilization Research and Development Division, Agricultural Research Service, U.S. Department of Agriculture. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Citing Literature Volume21, Issue41969Pages 92-96 RelatedInformation

Note on the Natural Occurrence of Ochratoxin A

Journal Article Note on the Natural Occurrence of Ochratoxin A Get access Odette L Shotwell, Odette L Shotwell Northern Regional Research Laboratory,1 Peoria, 111. 61604 Search for other works by this author on: Oxford Academic Google Scholar C W Hesseltine, C W Hesseltine Northern Regional Research Laboratory,1 Peoria, 111. 61604 Search for other works by this author on: Oxford Academic Google Scholar Marion L Goulden Marion L Goulden Northern Regional Research Laboratory,1 Peoria, 111. 61604 Search for other works by this author on: Oxford Academic Google Scholar Journal of Association of Official Analytical Chemists, Volume 52, Issue 1, 1 January 1969, Pages 81–83, https://doi.org/10.1093/jaoac/52.1.81 Published: 01 February 2020 Article history Received: 21 October 1968 Published: 01 February 2020

Stereospecific hydrogenation of stearolate with copper catalysts

Journal of the American Oil Chemists' SocietyVolume 45, Issue 10 p. 708-708 Technical Stereospecific hydrogenation of stearolate with copper catalysts Sambasivarao Koritala, Sambasivarao Koritala Northern Regional Research Laboratory, No. Utiliz. Res. Dev. Div., ARS, USDA, Peoria, Illinois, 61604Search for more papers by this author Sambasivarao Koritala, Sambasivarao Koritala Northern Regional Research Laboratory, No. Utiliz. Res. Dev. Div., ARS, USDA, Peoria, Illinois, 61604Search for more papers by this author First published: 01 October 1968 https://doi.org/10.1007/BF02541263Citations: 3AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat No abstract is available for this article.Citing Literature Volume45, Issue10October 1968Pages 708-708 RelatedInformation