Northern Queensland Research Articles

Exploring the occupational experiences of livestock farmers during drought: A narrative inquiry.

For much of the 21st century, Australia has endured the most prolonged and severe drought since records began. This drought has been found to have negative and long-lasting consequences upon both the physical and mental well-being of farmers and their families. To date, however, no research has examined the experience of drought from an occupational perspective. This study aims to explore the way in which drought impacts upon the lived experience of the farming role and the way in which the occupational identity of farmers influences the meanings attributed, and response to, drought. Narrative inquiry and thematic analysis were used to explore the experiences of drought among six farming men and four farming women from Northern Queensland. Four inter-related themes were identified. These being: 'Becoming a farmer - Rites of passage on entry to the farming role'; 'Farmers as guardians over the land'; 'Drought as siege' and; 'Leisure occupations as temporary bridges to a world beyond drought'. Each of these themes offer insights into the way in which drought comes to be understood by farmers and, in turn, experienced and responded to. By better understanding the occupational experiences of farmers during drought, resources can be more effectively targeted towards promoting occupational balance and well-being. Interventions aimed at reframing the way in which the farming role is conceptualised from a young age and supporting occupations beyond farming as bridges to the outside world may be effective in achieving positive outcomes during drought.

Occurrence of emerging contaminants in biosolids in northern Queensland, Australia

This study aims to identify and quantify different classes of emerging contaminants (ECs), such as pharmaceutical and personal care products (PPCPs), per-and polyfluoroalkyl substances (PFAS), heavy metals (HMs), polycyclic musks (PMs) in biosolids from different sewage treatment plants (STPs) from regional councils across Northern Queensland, Australia. Biosolids samples were named BS1 to BS7 for each council. The results revealed significant variations in the concentrations of different ECs in biosolids which could be explained in some instances by the characteristics of the upstream sewage network. For instance, BS4-biosolids from a small agricultural shire (largely sugarcane) showed the highest concentration of zinc and copper, which were 2430 and 1050 mg/kg, respectively. Among PPCPs, the concentration of ciprofloxacin was found to be the highest in BS3 and BS5, two large regional council areas which are a mix of domestic and industrial (predominantly domestic) biosolids of 1010 and 1590 ng/g, respectively. In addition, the quantity of sertraline was consistently high in all biosolids except from BS7, one of the smaller regional councils, which is indicative of the domestic catchments attached. PFAS compounds were detected in all biosolids samples except in BS6, one of the small (agricultural and tourist) catchments. Two PFAS compounds emerged as the most common pollutants that were perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS). The largest industrial catchment biosolids, BS2 showed the highest concentration of PFOS at 253 ng/g, while the smallest regional council, BS7 showed the maximum concentration of 7.90 ng/g of PFOA. Overall, this study concludes that certain ECs such as HMs, antibiotics, PFOS and PFOA in biosolids may pose high environmental risks.

Four species in one: taxonomic revision of the Eirmocides helenita (Semper, 1879) complex (Lepidoptera: Lycaenidae) from Australia and New Guinea

AbstractTaxonomic investigations of the Eirmocides helenita (Semper, 1879) complex from north‐eastern Australia and mainland New Guinea based on adult morphology (male genitalia and wing colour pattern elements), together with a phylogenetic hypothesis of the genus (18 or 69% of the recognised species) based on molecular data (1 mitochondrial and 12 nuclear genes), and examination of type material, indicate that there are five species within this monophyletic lineage, here referred to as the E. helenita species‐group. Four of the species, previously classified under the name Eirmocides helenita (Semper, 1879) sensu lato and characterised by iridescent turquoise dorsal colouration in the male, are predominantly allopatric, as follows: Eirmocides callainus Braby & Müller, sp. nov., is endemic to the Wet Tropics biome of north‐eastern Queensland; Eirmocides helenita (Semper, 1879) sensu stricto is restricted to Cape York Peninsula of northern Queensland; Eirmocides rouku Braby & Müller, sp. nov., is endemic to the Western Province of southern Papua New Guinea; and Eirmocides dimorphus (Röber, 1886), stat. rev., occurs more widely throughout mainland New Guinea and its adjacent islands. The fifth member of the group, Eirmocides cupreus (Röber, 1886), is sympatric with E. dimorphus on mainland New Guinea. The taxonomic revision brings to 26 the total number of species recognised in Eirmocides and within the tribe Candalidini to 40 species. Lectotypes are designated for Plebeius dimorphus Röber, 1886, Plebeius dimorphus var. cupreus Röber, 1886, and Holochila subargentea Grose‐Smith & Kirby, 1896.

Differences in gene expression in field populations of Wolbachia-infected Aedes aegypti mosquitoes with varying release histories in northern Australia.

Aedes aegypti is the principal mosquito vector of dengue, yellow fever, Zika and chikungunya viruses. The wMel strain of the endosymbiotic bacteria Wolbachia pipientis was introduced into the vector as a novel biocontrol strategy to stop transmission of these viruses. Mosquitoes with Wolbachia have been released in the field in Northern Queensland, Australia since 2011, at various locations and over several years, with populations remaining stably infected. Wolbachia infection is known to alter gene expression in its mosquito host, but whether (and how) this changes over the long-term in the context of field releases remains unknown. We sampled mosquitoes from Wolbachia-infected populations with three different release histories along a time gradient and performed RNA-seq to investigate gene expression changes in the insect host. We observed a significant impact on gene expression in Wolbachia-infected mosquitoes versus uninfected controls. Fewer genes had significantly upregulated expression in mosquitoes from the older releases (512 and 486 from the 2011 and 2013/14 release years, respectively) versus the more recent releases (1154 from the 2017 release year). Nonetheless, a fundamental signature of Wolbachia infection on host gene expression was observed across all releases, comprising upregulation of immunity (e.g. leucine-rich repeats, CLIPs) and metabolism (e.g. lipid metabolism, iron transport) genes. There was limited downregulation of gene expression in mosquitoes from the older releases (84 and 71 genes from the 2011 and 2013/14 release years, respectively), but significantly more in the most recent release (509 from the 2017 release year). Our findings indicate that at > 8 years post-introgression into field populations, Wolbachia continues to profoundly impact expression of host genes, such as those involved in insect immune response and metabolism. If Wolbachia-mediated virus blocking is underpinned by these differential gene expression changes, our results suggest it may remain stable long-term.

Biogeographic Variability in Kernel Oil and Press Cake Content of Beauty Leaf Tree (Calophyllum inophyllum L.), as Determined by Chemical and Near-Infrared Spectroscopy Analysis

The aim of this study was to evaluate biogeographic variability in the fruit, kernel, kernel-oil and press-cake contents of 50 accessions of the beauty leaf tree (C. inophyllum L.) collected from 19 locations spanning 4000 km along the eastern and northern coasts of Australia (Northern Territory and Queensland). Mature fallen fruits of C. inophyllum were collected from individual trees and stored in a shed for over a year. The fruits were cracked open to extract the kernels, and the kernels were crushed to 5–10 mm. NIR spectra of crushed kernels were collected using FT–NIR. Results of this study showed large variation between individual trees and the provenances for oil, resin and cake contents. Most of the C. inophyllum genotypes were separated based on their NIR fingerprint using PCA and PLS-DA. It was concluded that NIR spectroscopy not only aids in the screening of large numbers of genotypes, but it also allows the preservation of the tested seeds for further propagation. This feature will have the greatest advantage in plant breeding and commercial cultivation, as only the seeds that contain high oil content could be sown to help establish plantations with high oil-production capacity. Overall, it was concluded that the differences between provenances for oil, resin and cake contents can be predicted using NIR spectra. Furthermore, NIR spectroscopy can be used as a tool to define provenance variations in the kernel oil content of the beauty leaf tree.

Effect of seaweed extract on avocado root growth, yield and post-harvest quality in far north Queensland, Australia

Seaweed extracts are proven to increase productivity in many agricultural crops, but there is limited research on their use in avocado production. Therefore, we evaluated the effectiveness of a seaweed extract from Durvillaea potatorum and Ascophyllum nodosum on avocado yield, revenue and post-harvest fruit quality in a series of field experiments in Australia, and on seedling root growth in a pot experiment. The field experiments were conducted on commercial farms across three different locations in northern Queensland over four years and utilised avocado trees with different ages, cultivars (Hass and Shepard) and inoculum pressures from Phytophthora cinnamomi. Results showed that the application of the seaweed extract by fertigation significantly improved avocado yield (kg fruit per tree) by 38%, fruit firmness by 4% (skin) and 22% (flesh) and fruit skin colour by 1° (hue), and an upgraded visual ripeness score. The increases in yield were associated with greater number of fruits per tree (up to 42%) indicating the liquid seaweed extract improved fruit set and retention per tree. Regular soil application of the seaweed extract to young trees (cv. Hass) in pots increased the root fresh weight by 22%. Overall, the regular application of the seaweed extract to avocado trees was found to be practical and economically viable for improving fruit production and post-harvest quality in Australian orchards.

The infection efficacy of Metarhizium strains (Hypocreales: Clavicipitaceae) against the Queensland fruit fly Bactrocera tryoni (Diptera: Tephritidae).

The Queensland fruit fly (Qfly), Bactrocera tryoni Froggatt, is a devastating pest of Australia's commercial fruit systems. Fruit fly mitigation is heavily centered around the use chemical insecticides, with limited investigation into microbial control alternatives. The wet tropics of northern Queensland is a highly biodiverse ecosystem containing many species of insect pathogenic fungi, but it is unclear whether any of these entomopathogens could contribute to Qfly management programs. In laboratory trials, we investigated the potential for Qfly microbial control by 3 locally sourced strains of entomopathogenic fungi comprising 2 species, Metarhizium guizhouense (Chen and Guo) and Metarhizium lepidiotae (Driver and Milner). Additionally, we evaluated 2 different inoculation methods to derive the most effective way to expose the flies to conidia-either through dry conidia or in a conidial suspension. All 3 strains were successful in causing Qfly mortality. Metarhizium lepidiotae resulted in the highest mean mortality over the trials, while M. guizhouense resulted in the highest mortality in a single replicate. Laboratory experiments revealed exposure through dry conidia to be the most effective method to inoculate the flies. These results suggest that fungal entomopathogens could be a viable pathway to Qfly suppression.

Integrated GIS-AHP-based approach for off-river pumped hydro energy storage site selection

Pumped hydro energy storage (PHES) solutions enable greater diffusion of renewable energy into the electricity grid. However, accelerated development of PHES is complex due to the numerous spatially relevant technical, environmental, social, and economic criteria that must be assessed to determine a pumped hydro sites feasibility. With the goal to rapidly narrow down feasible sites from a large land area, this study developed a Geographic Information System (GIS) and Analytic Hierarchy Process (AHP) based technique to autonomously identify PHES sites based on a range of environmental and technical criteria. Following GIS-AHP analysis, Levelized Cost of Energy (LCOE) scenario analysis was conducted with a limited number of feasible sites, as well as their carbon abatement potential. The developed approach was demonstrated for the case of Northern Queensland (NrQLD), Australia. The developed GIS-AHP PHES site selection approach identified 14 potentially feasible sites. For the base case scenario, these sites could generate 366.94 TWh over their lifetime while abating 300 ktCO2eq. The LCOE of these 14 PHES sites ranged between 0.04 AU$/kWh and 0.27 AU$/kWh for the base case scenario. The developed approach has implications for energy planning managers seeking to efficiently narrow down feasible PHES sites, which are essential storage enablers for transitioning to a zero-emissions economy.

Influence of Landscape Characteristics on Wind Dispersal Efficiency of Calotropis procera

Rubber bush (Calotropis procera), a perennial invasive milkweed, infests large swathes of pastoral land in northern Australia and Queensland, diminishing pasture productivity. The seeds of rubber are small with fluffy pappi that confer buoyancy during wind dispersal. Long-distance seed dispersal (LDD) by wind is dependent in part on seed terminal velocity, the height of release above the ground, the surrounding vegetation, and wind parameters such as speed and vertical turbulence. Using empirical dispersal data, spatial population distribution, and historical knowledge of three experimental sites, we examine how seed traits can interact with environmental features to promote dispersal. We expected naturalised rubber bush populations to have the following: (1) higher spatial autocorrelation on open plains where dispersal distances are maximised compared to hilly habitats or those with tall vegetation; (2) southeast to northwest directional bias aligned to prevailing winds; and (3) patchy satellite populations ahead of an infilled continuous main front. Seed dispersal kernels were estimated by releasing seeds from dehiscent fruit for four periods of ten minutes each at three locations from a fixed height while monitoring wind speed. Five alternative models were fitted to the seed dispersal data, of which the log-logistic (Kolgomorov–Smirnov test p = 0.9998), 3-parameter Weibull model (K-S p = 0.9992), and Weibull model (K-S p = 0.9956) provided the best fit in that order. Stem size distribution was similar at the leading edges of populations at all sites (F10, 395 = 1.54; p = 0.12). The exponential semivariogram model of the level of spatial autocorrelation was the best fit and was adopted for all sites (Tennant Creek (TC), Helen Springs (HS) and Muckaty (MU) sites (R2 = 63.8%, 70.3%, and 93.7%, respectively). Spatial autocorrelation along the predicted southeast-to-northwest bearing was evident at all sites (TC kriging range = 236 m; HS = 738 m and MU = 1779.8 m). Seed dispersal distance was bimodal and dependent on prevailing wind conditions, with short distance dispersal (SDD) up to 55 m, while the furthest propagules were 1.8 km downwind in open environments. Dispersal directions and distances were pronounced on plains with short or no vegetation, compared to hilly locations or areas with tall vegetation. In designing management strategies, it should be noted that invasion risk is greater in frequently disturbed open landscapes, such as pastoral landscapes in Northern Australia. Infestations on open xeric grassland plains with shrubby vegetation should be a priority for rubber bush control to maintain high levels of productivity in beef production systems.

Cherax quadricarinatus Resistant to Chequa iflavirus: A Pilot Study.

High mortalities of redclaw crayfish (Cherax quadricarinatus) were reported from northern Queensland farms, mainly attributed to two viruses, Chequa iflavirus and Athtab bunyavirus. From a research population of redclaw crayfish with these pre-existing viral infections, five individuals were found uninfected by Chequa iflavirus but infected with Athtab bunyavirus. A pilot study was designed to examine if progeny crayfish from this cohort were resistant to infections by Chequa iflavirus. Two experiments measured changes in viral load with RT-qPCR. Seven donors, four negative controls and six crayfish injected with a purified virus or saline were used. In Experiment 1, the purified viral inoculum was injected into the crayfish, and they were bled 14 days post-injection (dpi). In Experiment 2, haemolymph containing the viruses was injected into the same crayfish and they were bled at 24 hpi, 48 hpi, 7 dpi and 14 dpi. In Exp. 1, the crayfish cleared Chequa iflavirus infections within 14 dpi, while in Exp. 2, it was within 24 hpi. One mortality was observed, but that crayfish had cleared the virus before dying. The number of copies of Athtab bunyavirus and the weights of the crayfish did not differ significantly (p > 0.05) between the control and injected crayfish. Histology of crayfish all showed that the haemolymph vessels were clear of granulomas, suggesting no bacterial involvement. There was no melanisation in the gill tissue of control crayfish, but it was prominent in virus-injected crayfish. Neither group had haemocytic infiltration of the muscle fibres. Anti-viral immune mechanisms of RNA interference and Cherax quadricarinatus Down Syndrome Cell Adhesion Molecule (DSCAM) are hypothesised to be involved in viral clearance. We conclude that these crayfish were resistant to Chequa iflavirus infections and could be commercially exploited by aquaculturists as a nuclear breeding stock if numbers are increased over time.

Time trends, projections, and spatial distribution of low birthweight in Australia, 2009-2030: Evidence from the National Perinatal Data Collection.

Infants with low birthweight (LBW, birthweight <2500 g) have increased in many high-resource countries over the past two decades. This study aimed to investigate the time trends, projections, and spatial distribution of LBW in Australia, 2009-2030. We used standard aggregate data on 3 346 808 births from 2009 to 2019 from Australia's National Perinatal Data Collection. Bayesian linear regression model was used to estimate the trends in the prevalence of LBW in Australia. Wefound that the prevalence of LBW was 6.18% in 2009, which has increased to 6.64% in 2019 (average annual rate of change, AARC=+0.76%). If the national trend remains the same, the projected prevalence of LBW in Australia will increase to 7.34% (95% uncertainty interval, UI=6.99, 7.68) in 2030. Observing AARC across different subpopulations, the trend of LBW was stable among Indigenous mothers, whereas it increased among non-Indigenous mothers (AARC=+0.81%). There is also an increase among the most disadvantaged mothers (AARC=+1.08%), birthing people in either of two extreme age groups (AARC=+1.99% and +1.53% for <20 years and ≥40 years, respectively), and mothers who smoked during pregnancy (AARC=+1.52%). Spatiotemporal maps showed that some of the Statistical Area level 3 (SA3) in Northern Territory and Queensland had consistently higher prevalence for LBW than the national average from 2014 to 2019. Overall, the prevalence of LBW has increased in Australia during 2009-2019; however, the trends vary across different subpopulations. If trends persist, Australia will not achieve the Sustainable Development Goals (SDGs) target of a 30% reduction in LBW by 2030. Centering and supporting the most vulnerable subpopulations is vital to progress the SDGs and improves perinatal and infant health in Australia.

Mid-career graduate practice outcomes of the James Cook University medical school: key insights from the first 20 years.

Previous studies demonstrate early-career James Cook University (JCU) medical graduates are more likely to practise in regional, rural and remote areas than other Australian doctors. This study investigates whether these practice patterns continue into mid-career, identifying key demographic, selection, curriculum and postgraduate training factors associated with rural practice. The medical school's graduate tracking database identified 2019 Australian practice location data for 931 graduates across postgraduate years (PGY) 5-14, which were categorised into Modified Monash Model (MMM) rurality classifications. Multinominal logistic regression was undertaken to identify specific demographic, selection process, undergraduate training and postgraduate career variables associated with practice in a regional city (MMM2), large to small rural town (MMM3-5) or remote community (MMM6-7). One-third of mid-career (PGY5-14) graduates were working in regional cities, mostly in North Queensland, with 14% in rural towns and 3% in remote communities. These first 10 cohorts were undertaking careers in general practice (n=300, 33%), as subspecialists (n=217, 24%), rural generalists (n=96, 11%), generalist specialists (n=87, 10%) or hospital non-specialists (n=200, 22%). The findings show positive outcomes from the first 10 JCU cohorts for regional Queensland cities, with a significantly higher proportion of mid-career graduates practising regionally compared with the overall Queensland population. The proportion of JCU graduates practising in smaller rural or remote towns is similar to the overall Queensland population. The establishment of the postgraduate JCUGP Training program and the Northern Queensland Regional Training Hubs for building local specialist training pathways should further strengthen medical recruitment and retention across northern Australia.

Camera trapping ekes out some improvement for surveying sparse mammal populations in northern Queensland

Monitoring is a critical component of conservation land management and the choice of methods can influence the final inventory of species recorded. The use of camera trapping has increased in recent years as a cost-effective method to record more species and to identify more cryptic and rare species. In this study we first examined data from detailed field surveys (which did not employ camera trapping) in northern Queensland to examine the abundance and frequency of mammals detected by cage, box and pitfall traps, and spotlighting. We then used data from an additional set of sites that compared these methods with camera trapping. Twenty-five species were recorded in the first data set and 26 species were recorded in the second. Overall, mammals were recorded in exceedingly low numbers, and camera trapping only improved the detection of some species such as larger species that could not be trapped (i.e. dingo Canis familiaris and pig Sus scrofa) or were uncommon (i.e. northern quoll Dasyurus hallucatus and northern brown bandicoot Isoodon macrourus). Our results suggest that survey effort should be substantial and use the most suitable methods to identify management, threat and habitat relationships for potentially precarious wildlife communities.

New records and a review of animal foods in the diets of the Brolga Antigone rubicunda and Australian Sarus Crane A. antigone gillae

Cranes are opportunistic omnivores, sometimes considered to be primarily vegetarian. However, there is limited knowledge in Australia of their animal prey and its importance in their diets. From field records in 2006–2021, we report novel foods including fish and crabs taken by adult Australian Sarus Cranes Antigone antigone gillae and the first records of the foods of their dependent young (crabs and grasshoppers). For Brolgas A. rubicunda, we report the first records of ducklings as prey, and of crabs, beetles and grasshoppers fed to dependent young. Both species have learnt to prey on rodents displaced or killed by sugar cane harvesting machinery in northern Queensland. A review of diet records since 1810 also reveals some overlooked foods and food-handling methods but, despite a sighting of a Brolga ingesting a whole Cane Toad Rhinella marina, there is no evidence of immunity to the toad’s toxin in either species of crane. The plant and animal components of the diet of the dependent young of both species remain almost unknown and there are also significant knowledge gaps for the animal prey of adult Australian Sarus Cranes; the vertebrate prey of adult Brolgas; and the balance of prey types for both species in the breeding and non-breeding seasons. To advance knowledge of crane diets and nutritional ecology in Australia and New Guinea, direct field techniques including faecal sampling, collection of shed feathers and camera trapping could be undertaken, supported by remote electronic technologies in relatively inaccessible breeding wetlands. Meanwhile – in anticipation of the forthcoming revision of the Handbook of Australian, New Zealand &amp; Antarctic Birds – we urge fieldworkers, birdwatchers and others to examine their field notes for unpublished information on crane foraging and food items, especially for the dependent young of Brolgas and Australian Sarus Cranes.

Population dynamics of chital deer (Axis axis) in northern Queensland: effects of drought and culling

Context Chital deer (Axis axis) are long established in the northern Queensland dry tropics, and at high densities are considered pests by cattle graziers. Cost-effective management is difficult for widespread, fluctuating populations of vertebrate pests such as these deer. Historically, control of chital deer has been limited to recreational and some commercial ground-shooting and trapping. Concerns over chital deer impacts were heightened during drought in 2015 and funding became available for aerial culling. Aim This study set out to determine (1) distribution and abundance, (2) seasonal reproductive output, (3) potential and actual rates of increase and their determinants, and (4) efficient management strategies for chital deer in the northern Queensland dry tropics. Methods In 2014, ~13 000 km2 of the main distribution was surveyed by helicopter. Multiple vehicle ground surveys per year monitored chital deer density on two properties during 2013–2022. Seasonal shot samples of deer on both properties assessed reproductive output during 2014–2016. Aerial surveys during 2016–2020 determined chital deer densities on seven properties, prior to aerial culling on four properties. Finally, the maximum rate of increase of chital deer was calculated from life-history data. Key results Regionally, chital deer are patchily distributed and so are best monitored locally where densities can be &gt;50 deer km−2. Vehicle ground surveys recorded an ~80% decline in chital deer populations on two properties over 7–10 months during drought in early 2015, with a similar rate being recorded by aerial survey at a third site. There was little recruitment during the drought, but the decline was seemingly driven by adult mortality. Aerial shooting further reduced populations by 39–88% to &lt;3 deer km−2 on four properties. Where there was no continuing control, culled populations recovered to pre-cull densities or higher after 2.4 years. One unculled property recovered to its pre-drought density after 6 years. Rates of recovery were at or higher than the maximum annual rate of increase for chital deer estimated here as 26–41%. Conclusions Drought has a lasting effect on this chital deer population, because of the resulting large population decline and the modest rate of any recovery in the absence of culling. Culling can reduce populations to low density, but the removal rate needs to be sustained to suppress future growth. Implications Drought provides a strategic opportunity to further reduce chital deer populations for enduring control. Large reductions are feasible given the clumped dispersion of populations within properties and in the region.

Genomic analysis of an outbreak of toxin gene bearing Corynebacterium diphtheriae in Northern Queensland, Australia reveals high level of genetic similarity.

Toxigenic diphtheria is rare in Australia with generally fewer than 10 cases reported annually; however, since 2020, there has been an increase in toxin gene-bearing isolates of Corynebacterium diphtheriae cases in North Queensland, with an approximately 300% escalation in cases in 2022. Genomic analysis on both toxin gene-bearing and non-toxin gene-bearing C. diphtheriae isolated from this region between 2017 and 2022 demonstrated that the surge in cases was largely due to one sequence type (ST), ST381, all of which carried the toxin gene. ST381 isolates collected between 2020 and 2022 were highly genetically related to each other, and less closely related to ST381 isolates collected prior to 2020. The most common ST in non-toxin gene-bearing isolates from North Queensland was ST39, an ST that has also been increasing in numbers since 2018. Phylogenetic analysis demonstrated that ST381 isolates were not closely related to any of the non-toxin gene-bearing isolates collected from this region, suggesting that the increase in toxigenic C. diphtheriae is likely due to the expansion of a toxin gene-bearing clone that has moved into the region rather than an already endemic non-toxigenic strain acquiring the toxin gene.

High incidence of inflammatory bowel disease in Northern Australia: a prospective community population-based Australian incidence study in the Mackay-Isaac-Whitsunday region.

To determine the incidence of inflammatory bowel disease (IBD) in the Mackay-Isaac-Whitsunday region in Northern Queensland (-21.14° S) and to allow a comparison with Southern Australian and New Zealand data (Geelong, Australia -38.14° S; Tasmania -41.43° S and -42.88° S (Launceston and Hobart) and Canterbury, New Zealand -43.46 °S). A prospective observational community population-based IBD study was conducted between 1 June 2017 and 31 May 2018. Primary includes the crude annual incidence rate of IBD, Crohn's disease (CD), ulcerative colitis (UC) and inflammatory bowel disease-unclassified (IBDU), while secondary includes disease phenotype and behaviour. Fifty-six new cases of IBD were identified. Twenty-three were CD, 30 were UC and 3 were IBDU. The crude annual incidence rate per 100 000 for IBD, CD, UC and IBDU were 32.2 (95% confidence interval (CI): 24.78-41.84), 13.23 (95% CI: 8.79-19.90), 17.25 (95% CI: 12.06-24.67) and 1.73 (95% CI: 0.56-5.35). When directly age-standardised to the World Health Organisation Standard Population Distribution, the overall CD, UC and IBDU incidence were 13.19, 17.34 and 1.85 per 100 000, with an overall age-standardised IBD incidence of 32.38. This is the first study to define the incidence of IBD in a Northern Australian cohort and to allow a comparison between North and Southern Australia. The IBD crude is the highest reported in Australia. Like others, we found a high and low incidence of upper gastrointestinal Crohn's disease and complicated disease at diagnosis respectively, likely reflective of the increased availability and early uptake of endoscopic procedures.

Genetic Diversity and Expanded Host Range of J Paramyxovirus Detected in Wild Small Mammals in China

J paramyxovirus (JPV) is a rodent-borne Jeilongvirus isolated from moribund mice (Mus musculus) with hemorrhagic lung lesions trapped in the 1972 in northern Queensland, Australia. The JPV antibodies have been detected in wild mice, wild rats, pigs, and human populations in Australia. Here, by next-generation sequencing (NGS), we detected JPV from M. musculus in Shandong Province of China. Molecular detection of JPV was performed to survey to survey the infection among 66 species of wild small mammals collected from six eco-climate regions in China by applying JPV specific RT-PCR and sequencing. Altogether, 21 out of 3070 (0.68%) wild small mammals of four species were positive for JPV, including 5.26% (1/19) of Microtus fortis, 3.76% (17/452) of M. musculus, 1.67% (1/60) of Apodemus peninsulae, and 0.48% (2/421) of Apodemus agrarius, which captured three eco-climate regions of China (northeastern China, northern China, and Inner Mongolia-Xinjiang). Sequence analysis revealed the currently identified JPV was clustered with other 14 Jeilongvirus members, and shared 80.2% and 89.2% identity with Australia's JPV partial RNA polymerase (L) and glycoprotein (G) genes, respectively. Phylogenetic analysis demonstrated the separation of three lineages of the current JPV sequences. Our results show three new hosts (A. agrarius, A. peninsulae, and M. fortis) for JPV, most of which were widely distributed in China, and highlight the potential zoonotic transmission of JPV in humans. The detection of JPV in wild small mammals in China broaden the viral diversity, geographical distribution, and reservoir types of JPV. Future studies should prioritize determining the epidemiological characteristics of JPV, so that potential risks can be mitigated.

Recircumscription of Utricularia leptorhyncha and U. lasiocaulis and three related new species for northern Australia

The Utricularia lasiocaulis F.Muell. complex (subg. Polypompholyx; sect. Lasiocaules) is a morphologically and ecologically variable group of closely related taxa with a mostly tropical distribution across northern Australia. A recent molecular phylogenetic study placed the recognised species U. kamienskii F.Muell. sister to a clade of accessions relegated to either U. leptorhyncha O. Schwarz or U. lasiocaulis, with the latter previously circumscribed as an assemblage of highly variable morphological forms. We have expanded the previous study to include populations representing the known distributions of all three species and have attempted to include all morphological variants; 55 ingroup accessions were used in the full phylogenetic analysis based on two non-coding chloroplast regions (rps16, trnD–T) and the nuclear ribosomal internal transcribed spacer (ITS). We found that the pink corolla form of the usually white flowered U. kamienskii is not sufficiently different, and we retain it under that species. We also found strong support for a paraphyletic U. leptorhyncha, with the smaller flowered accessions matching the type material placed sister to all other U. lasiocaulis forms, including a grouping previously assigned as a larger flowered U. leptorhyncha. Within the U. lasiocaulis clade we found that much of the variation sorts into well-supported clades that we find are sufficiently morphologically and genetically differentiated from the type clade for recognition at the specific rank, namely Utricularia brennanii R.W.Jobson &amp; Baleeiro from Northern Territory and Queensland, Utricularia cowiei R.W.Jobson &amp; Baleeiro from the Kimberley region of Western Australia, and U. disjuncta R.W.Jobson &amp; Baleeiro restricted to the Darwin and Gulf region of the Northern Territory.

Resistance Bioassays and Allele Characterization Inform Analysis of Spodoptera frugiperda (Lepidoptera: Noctuidae) Introduction Pathways in Asia and Australia.

The fall armyworm (FAW) Spodoptera frugiperda (Smith; Lepidoptera: Noctuidae) is present in over 70 countries in Africa, Asia, and Oceania. Its rapid dispersal since 2016 when it was first reported in western Africa, and associated devastation to agricultural productivity, highlight the challenges posed by this pest. Currently, its management largely relies on insecticide sprays and transgenic Bacillus thuringiensis toxins, therefore understanding their responses to these agents and characteristics of any resistance genes enables adaptive strategies. In Australia, S. frugiperda was reported at the end of January 2020 in northern Queensland and by March 2020, also in northern Western Australia. As an urgent first response we undertook bioassays on two Australian populations, one each from these initial points of establishment. To assist with preliminary sensitivity assessment, two endemic noctuid pest species, Helicoverpa armigera (Hübner; Lepidoptera, Noctuidae) and Spodoptera litura (Fabricius; Lepidoptera, Noctuidae), were concurrently screened to obtain larval LC50 estimates against various insecticides. We characterized known resistance alleles from the VGSC, ACE-1, RyR, and ABCC2 genes to compare with published allele frequencies and bioassay responses from native and invasive S. frugiperda populations. An approximately 10× LC50 difference for indoxacarb was detected between Australian populations, which was approximately 28× higher than that reported from an Indian population. Characterization of ACE-1 and VGSC alleles provided further evidence of multiple introductions in Asia, and multiple pathways involving genetically distinct individuals in Australia. The preliminary bioassay results and resistance allele patterns from invasive S. frugiperda populations suggest multiple introductions have contributed to the pest's spread and challenge the axiom of its rapid 'west-to-east' spread.