The diel periodicity of vertical migrations of herbage-dwelling spiders has been studied in natural habitats of clay semidesert of the northern Caspain (Transvolga) region. Steppe habitats (microdepressions) and desert habitats (microelevations) are shown to have much in common: the abundance and the family composition of spider populations of both biotopes are similar; they slightly differ only during the hot summer season. The amplitude of diel fluctuations in spider abundance is relatively great, which is typical of open cenoses under semiarid conditions. In spring and autumn, the peaks of spider abundance in both types of habitats are at night, when air temperature is minimal. In summer, in addition to the nighttime abundance peaks, there are daytime peaks of similar size, due mostly to the increased activity of “southern” taxa, the Thomisidae in desert associations and the Thomisidae + Salticidae in steppe associations. The diel periodicity of vertical migrations in hortobiontic spiders is a complicated phenomenon determined by a number of factors. It is to some extent conditioned by environmental parameters and by vertical migrations of their potential prey, phytophagous insects. Thus, the ecological niches in spiders of different taxa are separated in time according to their morphological and behavioral adaptation to climatic conditions. This separation probably alleviates competition between populations of different taxa.