An experiment was conducted to study the “Effect of zinc and boron on grain quality of wheat (Triticum aestivum L.) during the winter (rabi) season of 2018–2019 and 2019–20 at the agronomy research farm of IFTM University, Lodhipur Rajput, Moradabad, Uttar Pradesh. Ten treatments, viz. T1 , Control (no fertilizer); T2 , zinc @ 5 kg/ha (SA); T3 , zinc @ 1 kg/ha (FA); T4 - zinc @ 1 + 1 kg/ha (2 FA); T5 , zinc @ 5 kg/ha (SA) + zinc @ 1 kg/ha (FA); T6 , boron @ 0.5 kg/ha (SA); T7 , boron @ 0.1 kg/ha (FA); T8 , boron @ 0.2 kg/ha (2 FA); T9 , boron @ 0.5 kg/ha (SA) and boron @ 0.1 kg/ha (FA) and T10-, zinc @ 5 kg/ha + boron @ 0.5 kg/ha (SA) were comprised in randomized block design (RBD) with the three replications. The results revealed that different levels and application methods of zinc and boron significantly improved the grain quality of wheat. Maximum Zn content (35.4 and 35.9 mg/ha) and Zn uptake (185.1 and 197.1 g/ha) in 2018–19 and 2019–20, respectively were recorded with Zn @ 5 kg/ha (SA) + zinc @ 1 kg/ha (FA). Moreover, maximum B content (58.5 and 59.5 mg/ha) and B uptake (361.9 and 376.4 g/ha) in 2018–19 2019–20, respectively were observed with B @ 0.5 kg/ha (SA) and B @ 0.1 kg/ha (FA). Maximum protein content in grain (12.6 and 12.7%) and protein yield (712.8 and 744.9 kg/ha) was recorded under Zn - 5 kg/ha (SA) + B 0.5 kg/ha (SA) in 2018–19 and 2019–20, respectively