In June 2004, potato plants of cvs Bistra, Desiree, Discovery, KIS94-1/514 and P s ata, showing unusual virus-like symptoms, were observed in a field in central Slovenia. Crops from different parts of the country were planted in this field to compare the frequency of virus infections. The symptoms were observed only on potatoes originating from Raki c an in northeastern Slovenia. Affected plants showed severe dwarfing, leafcurling and reduced leaf size; tubers were very small and few in number. Brown spots were clearly visible in cross-sections of tubers. Sap from plants with symptoms was used for mechanical inoculation of test plants. Local and systemic symptoms were observed on Nicotiana benthamiana , N. clevelandii , N. glutinosa , N. rustica , N. tabacum , but only local lesions occurred on Datura stramonium . No symptoms were observed on Chenopodium quinoa . Electron microscopy of negatively stained crude extracts from N. benthamiana , N. clevelandii and N. tabacum with symptoms revealed numerous rhabdovirus-like particles. During the previous summer, tomato plants cv. Belle, showing unusual symptoms of distinct chlorotic spots, wrinkles on fruits and narrow, necrotic leaves, were observed in northeastern Slovenia, about 16 km from Raki c an. Infected plants also showed size reduction, curling of mature leaves, yellowing and chlorosis along the margins of younger leaves. Electron microscopy of negatively stained crude extracts revealed rhabdovirus-like particles. Three weeks after mechanical inoculation with extracts from leaves from affected plants, systemic yellowing of veins and leaf curling was observed on N . tabacum cv. White Burley, and necrotic spots, starting on the bases of the leaves, on N . clevelandii . These symptoms resembled those of potato isolate. Electron microscopy of crude sap extracts again revealed rhabdovirus-like particles . DAS-ELISA was used for confirmation of rhabdovirus infection of potato, tomato and affected plants of N. rustica inoculated with potato sap and N. tabacum cv. White Burley inoculated with tomato sap. Antisera against Eggplant mottled dwarf virus (EMDV) and Potato yellow dwarf virus (PYDV) were kindly supplied by Dr S. Winter, DSMZ, Braunschweig, Germany, and ELISA results confirmed that tested plants were infected with EMDV. No infections with PYDV were found. Although EMDV is widespread in the Mediterranean region and the Middle East (Brunt et al ., 1996), and has recently been reported in mainland Spain (Aramburu et al ., 2006), this is the first report of EMDV in Slovenia.