North Carolina State University College Research Articles

Postmortem pathologic findings in dogs that underwent total body irradiation and hematopoietic cell transplant: A case series of five dogs with B-cell multicentric lymphoma.

Alternative therapies that can help achieve complete remission in dogs with lymphoma include total body irradiation and hematopoietic cell transplant, though there are few reports describing successes and pathologic sequelae of these procedures. During a 10-year period, 94 dogs with multicentric lymphoma received a hematopoietic cell transplant following total body irradiation at North Carolina State University College of Veterinary Medicine. Seven of these 94 dogs (7%) died prior to discharge, five (5%) of which presented for postmortem examination. Of these dogs, four received an autologous hematopoietic cell transplant, while one received a haploidentical allogeneic hematopoietic cell transplant. All five dogs had bone marrow depletion with all hematopoietic lines affected. Three had systemic candidiasis, while two had bacterial infections. To the authors' knowledge, this is the first report to document pathologic findings and development of systemic mycoses in dogs post total-body irradiation therapy and hematopoietic cell transplant.

The Transition from Letter Grading to Modified Pass-Fail Grading at a College of Veterinary Medicine: A Narrative Inquiry of Student Experiences

Compared with traditional letter grading, pass/fail grading is an assessment approach that can alter the educational environment and enhance student wellbeing without compromising education quality. Little is known about the experiences of students during the transition from traditional grading to pass/fail grading. The onset of the COVID-19 pandemic resulted in an abrupt move to modified pass-fail (MPF) grading at North Carolina State University College of Veterinary Medicine (NCSU-CVM), followed by a decision to permanently adopt MPF grading for the entire core pre-clinical doctor of veterinary medicine (DVM) curriculum. This study employed a narrative inquiry of surveys and interviews to facilitate deep understanding of student perspectives during the transition to MPF grading. Focus was placed on understanding what this transition meant for DVM students in terms of life and learning quality. Our analysis identified seven key themes that captured student experiences, which were generally positive, during this transition: education culture, student perceptions of instructor impact, shift from extrinsic to intrinsic valuation of curricular content, competitiveness for external merit-based opportunities, use of letter grading and MPF in a single semester, student recommendations, and wellbeing. Through exploration of these themes and presentation of concerns identified in students’ stories, this study provides guidance for other programs considering revision of their own assessment frameworks.

Success in Grateful Client Philanthropy: Insights from Veterinary Faculty at North Carolina State University

The veterinary literature contains few publications or empiric research on the impact of grateful client philanthropy. To improve our understanding of the impact of philanthropy on veterinary medicine, we surveyed the faculty at NC State University College of Veterinary Medicine. This qualitative study involved an anonymous survey of 153 North Carolina State University College of Veterinary Medicine faculty members administered and analyzed through an online survey software program, QualitrixXMTM. 33% (51) of faculty responded to the survey. 73% (37) of the respondents indicated that their personal interactions with clients led to gifts to their research or service and 29% (15) indicated that they have received six or more gifts from their interactions with clients of the hospital. The top three examples of direct beneficial impacts from grateful client giving were research support (13.1%), technological improvements and equipment upgrades (10.5%), and professional development (4.6%). The top three negative impacts of faculty interactions with a grateful client program were the perceived need to address the overwhelming needs of the donor (19%), the faculty’s concerns regarding the owner’s higher expectations of service (7.1%) and time (7.1%). 61.9% of the respondents did not note any negative impacts. Top three responses to donor inquiries regarding philanthropic giving were to direct the client to a development officer (76%), discuss gift opportunities with the client (45.1%), and discuss individual research needs and opportunities (19.1%). Overall, faculty reported positive impact with grateful patient philanthropy. Continued development and understanding of the philanthropic process is recommended to maximize donor support of academic medical efforts.

MRI findings associated with clinical presentation and histopathology in a Mexican redknee tarantula (Brachypelma hamorii)

AbstractA 15‐year‐old, 9 g, female Mexican redknee tarantula (Brachypelma hamorii) presented to the North Carolina State University College of Veterinary Medicine with a history of anorexia and weight loss. Physical examination revealed an inability to extend the left chelicera, which was suspected to prevent the tarantula from prehending live prey. Magnetic resonance imaging revealed enlargement and T2 hyperintensity of the left chelicera. The patient was provided with supplemental feedings and rehydration until it was found deceased more than 8 months after the initial presentation. A postmortem examination was performed, and histopathology showed an area of chronic inflammation at the level of the left chelicera. Magnetic resonance imaging can be used as an antemortem diagnostic tool to identify lesions in tarantulas.

EVALUATION OF PROGNOSTIC INDICATORS FOR INJURED TURTLES PRESENTING TO A WILDLIFE CLINIC.

The Turtle Rescue Team (TRT) at North Carolina State University College of Veterinary Medicine currently sees over 500 chelonian cases a year. Each turtle receives a thorough physical examination as the main evaluation of its health because further diagnostics are cost prohibitive in a donation-based facility. Thus, there is a need to further understand how a few economical diagnostics can evaluate the prognosis of chelonians. The objectives were to evaluate lactate, PCV, total solids, glucose, temperature, and HR as prognostic indicators in multiple chelonian species and to determine how the change in lactate concentrations over a 24 h period affects prognosis. Eastern box turtles (Terrapene carolina carolina), yellow-bellied sliders (Trachemys scripta scripta), river cooters (Pseudemys concinna), painted turtles (Chrysemys picta), and common snapping turtles (Chelydra serpentina) were evaluated because of prevalence of presentation to the TRT, differences in habitat, and differences in physiology. Hyperlactatemia, an increased lactate over 24 h when the initial lactate concentration was greater than 5 mM/L, and anemia were determined to be significant prognostic indicators of mortality for all turtles, and hypoglycemia was a significant prognostic indicator of mortality for male turtles (P < 0.05).

Impact of Classical Counterconditioning (Quiet Kennel Exercise) on Barking in Kenneled Dogs-A Pilot Study.

Simple SummaryBarking is a major source of noise pollution in dog kennels and negatively impacts the welfare of anyone within earshot, especially the dogs in the kennels themselves. It is crucial to have solutions to help reduce barking quickly and humanely that are easy to understand and put into place that also do not require a lot of resources such as time, expertise, or money. This study looked at the use of an exercise (Quiet Kennel Exercise—QKE) that uses classical counterconditioning (Pavlov) to help change the way the dogs feel when a person enters the kennel area from unpleasant to pleasant. This should help to reduce barking, especially that which is caused by negative emotions such as fear and frustration. After the initial baseline period of 5 days, people passing through the kennel tossed treats to the dogs no matter what the dogs did for 10 days. Data was collected three times a day for the entire study period. Sound level readings (decibels), number of dogs present, and number of dogs barking were recorded. Results showed improvement in the loudest volume recorded after the exercise was in place, fewer dogs barking over time, and each dog barking less each time. The most improvement was noticed in the afternoon.Excessive barking is a major source of noise pollution in dog kennels and negatively impacts welfare. Because resources are often limited, minimizing barking in the simplest and most easily implementable way is imperative. This pilot study implemented a Quiet Kennel Exercise (QKE) that utilized classical counterconditioning to change the dogs’ negative emotional state (which can lead to barking) to a more positive emotional state. Therefore, barking motivation is reduced, so barking should decrease. This study aims to show proof of concept that decreasing barking through classical counterconditioning is effective. It was conducted in one ward of day-time boarding kennels at North Carolina State University College of Veterinary Medicine. Data was collected three times per day and included decibel readings, number of dogs present, and number of dogs barking during a 5-day initial baseline and 10-day intervention period. During baseline, people passing through the ward acted as they normally would. During intervention, passersby were asked to simply toss each dog a treat regardless of the dogs’ behaviors in the kennel. Descriptive results show improvement in maximum level of barking after QKE, fewer dogs barking over time, dogs barking less each time, and the most improvement noted in the afternoon.

Uneven Paths: Agricultural Pathways that Lead Students to Enroll

For colleges of agriculture throughout the US, recruitment and retention of undergraduate students is a matter of existential importance. We analyzed personal statements written by applicants accepted to undergraduate degrees at North Carolina State University College of Agriculture and Life Sciences (CALS) to determine what pre-university agricultural experiences are related to first-term success and graduation within six years of first enrollment. The 491 students who submitted written materials as a part of their application to CALS described an array of agriculture pathways that we classified as categories of work and volunteer experience, clubs, coursework, personal history, and leisure. Our study found that 319 students, or 65%, described at least one pathway that played a role in their choice to apply. We found that most pathways were dominated by White students, with just 8.8% of students with at least one agriculture pathway from a minoritized group. Further, we calculated the mean First Term GPA (FTGPA) and graduation rate after six years for each pathway group. Findings include that students who discussed FFA participation had relatively high FTGPAs and graduation rates, while students intending to become veterinarians had relatively high FTGPA but low six-year graduation rates.

Absence of known feline MYH7 and MYBPC3 variants in a diverse cohort of cats with hypertrophic cardiomyopathy.

Hypertrophic cardiomyopathy (HCM) is the most common cause of heart disease in the domestic cat with a genetic predisposition in a few breeds. In the Maine Coon and Ragdoll breeds, two variants associated with the HCM phenotype have been identified in the cardiac myosin binding protein C gene (MYBPC3; p.Ala31Pro and p.Arg820Trp respectively), and a single variant has been identified in the myosin heavy chain gene (MYH7; p.Glu1883Lys) in one domestic cat with HCM. It is not known if these variants influence the development of HCM in other cohorts of the feline population. The objective of this study was to evaluate the presence of the known MYBPC3 and MYH7 variants in a population of cats with HCM. DNA was isolated from samples collected from non-Ragdoll and non-Maine Coon domestic cats diagnosed with HCM through the North Carolina State University College of Veterinary Medicine and genotyped for the three variants. One-hundred and three DNA samples from cats with HCM were evaluated from domestic shorthair, domestic longhair and purebred cats. All samples were wt for the MYBPC3 and MYH7 variants. Although this study was limited by its inclusion of cats from one tertiary hospital, the lack of these MYBPC3 and MYH7 variants in this feline HCM population indicates that the clinical utility of genetic testing for these variants may be isolated to the two cat breeds in which these variants have been identified. Further studies to identify the causative variants for the feline HCM population are warranted.

TEMPORAL PATTERNS IN ADMISSION OF EASTERN BOX TURTLES (TERRAPENE CAROLINA CAROLINA) AT A NORTH CAROLINA WILDLIFE CLINIC AS A REFLECTION OF CLIMATE

Significant impacts of global climate change on wildlife have been documented and are projected to continue. Reptiles have been suggested as being especially susceptible to these effects along with other anthropogenic impacts on their environment. A retrospective review of medical records for 2,356 wild eastern box turtles (Terrapene carolina carolina) admitted to the Turtle Rescue Team (TRT) at the North Carolina State University College of Veterinary Medicine between 1996 and 2017 was performed in order to: 1) report common presenting complaints, 2) describe the timeline of when box turtles were admitted to the TRT for each year of the study, and 3) investigate temporal shifts in turtle admissions and associations with changes in environmental temperature over a 22-yr period. The most common presenting complaint was vehicular trauma (n = 1,195) with over 70% of the caseload associated with anthropogenic impacts. Average monthly temperatures from 1996 to 2017 for Raleigh, North Carolina, USA, were extracted from the National Oceanic and Atmospheric Administration climate database. By comparing the pre- and post-2006 years using the Mann-Whitney test, we found that both the annual peak temperature and the annual timing of admission to the TRT have shifted significantly or with a strong tendency towards significance (P = 0.0008 and 0.052, respectively). Annual peak temperature has increased by 1.3°C, and timing of admission has shifted 18 days earlier between pre- and post-2006 years. This supports the hypothesis that box turtle activity patterns are shifting and that these changes are potentially related to climate.

Chromatophoroma in a Siamese fighting fish (Betta splendens)

An adult male blue Siamese fighting fish (Betta splendens) presented to the North Carolina State University College of Veterinary Medicine with a cranial mass located dorsal to his right eye....

Ecological and Socioeconomic Factors Associated with Bartonella henselae Exposure in Dogs Tested for Vector-Borne Diseases in North Carolina.

Bartonella henselae is a zoonotic vector-borne pathogen affecting both humans and dogs. Little is known about the epidemiology of B. henselae in dogs, including risk factors associated with exposure. The objectives of this study were to map the current distribution of B. henselae in dogs in North Carolina (NC) and to identify ecological and socioeconomic factors influencing B. henselae seroreactivity. Results from 4446 B. henselae serology samples from dogs in NC submitted by veterinarians for clinical diagnostic testing to the North Carolina State University College of Veterinary Medicine Vector Borne Disease Diagnostic Laboratory between January 1, 2004 and December 31, 2015 were retrospectively reviewed. These results were used to generate a map of B. henselae seroreactivity. To account for sparsely sampled areas, statistical smoothing using head banging and areal interpolation kriging was performed. Using previously described risk factors for exposure to canine tick-borne diseases, eight multivariable logistic regression models based on biologically plausible hypotheses were tested, and a final model was selected using an Akaike's Information Criterion weighted-average approach. Seroreactivity among dogs tested for vector-borne disease was variable across the state: higher along the southern/eastern coastal plains and eastern Piedmont, and lower in the western mountains. Of 25 explanatory factors considered, the model combining demographic, socioeconomic, climatic, and land use variables fits best. Based on this model, female intact sex and increasing percentage of the county with low-intensity development and evergreen forest were associated with higher seroreactivity. Conversely, moderate development, increasing median household income, and higher temperature range and relative humidity were associated with lower seroreactivity. This model could be improved, however, by including local and host-scale factors that may play a significant role in dogs' exposure.

Grateful Client Philanthropy and Veterinary Medicine: Experiences at North Carolina State University.

The historical reliance of state and federal funds as a sole source of veterinary educational activities has created a funding gap at many academic institutions. Due to declining resources, philanthropy has become an important source of financial support for veterinary colleges in the United States. In particular, for academic institutions with veterinary hospitals, grateful client philanthropy has been an increasingly important area of resource growth. Philanthropic gifts support innovative research, scholarship and capital, and programmatic initiatives. Areas of giving are often geared towards major infrastructure gifts and naming opportunities, faculty endowment, student scholarships, and other gift opportunities. This review provides an overview of grateful client philanthropy at North Carolina State University College of Veterinary Medicine and explores the various giving opportunities and challenges of donor giving in veterinary medicine. (129/200)

Ten years of experience with a veterinary credential responder course

Training of veterinary students to improve their ability to respond to disaster events that affect livestock and companion animals is an important facet of veterinary education. Just over 10 years ago, the North Carolina (NC) State University College of Veterinary Medicine worked collaboratively with the NC Department of Agriculture and Consumer Service's Emergency Management Program and the University of North Carolina at Chapel Hill Gillings School of Global Public Health, to develop a competency-based Veterinary Credential Responder (VCR) course. This special report reflects on this experience and provides a detailed description of the current VCR course. The 2-week VCR course combines lecture, online, experiential, and group exercises to meet entry-level federal credentialing requirements. Students gain a working knowledge of emergency management, emergency operation plans, and emergency support functions. Over 1000 veterinary students have received the VCR credential making them eligible to participate as deployable members of NC Veterinary Response Corps.

ASSESSMENT OF A VISUAL SCORING SYSTEM FOR IDENTIFYING AND QUANTIFYING ANEMIA IN MALE EASTERN BOX TURTLES ( TERRAPENE CAROLINA CAROLINA).

The North Carolina State University College of Veterinary Medicine Turtle Rescue Team (TRT) regularly admits wild eastern box turtles (EBT) ( Terrapene carolina carolina) for treatment and rehabilitation. Adult males of this species generally have an impressive red coloration of the iris, a feature commonly used to differentiate them from their brown-eyed female conspecifics. Male EBTs that present often display marked pallor of the eyes, which has accompanied clinical anemia. The aim of this study was to examine the relationship between eye color and packed cell volume (PCV) in adult male EBTs, and to assess the accuracy of using eye color to predict PCV. Eye-color scoring cards were created using Adobe Creative Cloud Photoshop®. Each card was composed of blocks displaying progressively lighter tints of a red hue, with a number (score) assigned to each block. Five-block, six-block, and nine-block scoring cards were prepared, with lower numbers (scores) corresponding to lighter hues. To determine the variation in subjective color scores assigned by different human observers, 85 individuals were asked to score EBT eye color in three animals using each of the three scoring cards. Eye color of adult male EBTs that presented to the TRT ( n = 18) and those housed at the North Carolina Museum of Natural Sciences ( n = 6) were scored using each of the three cards, immediately preceding venipuncture of the occipital sinus for determination of PCV. All animals were scored by at least two individuals. Turtles with low scores (pale eyes) using all three scoring cards consistently had lower PCVs. The five- and six-block scorecards had a positive predictive value (PPV) of 100% while the nine-block score card had a PPV of 71.4%. This specific and noninvasive method for estimating PCV of male EBTs is clinically desirable in both rehabilitation and hospital settings.

Gross Morphometry, Histomorphometry, and Immunohistochemistry Confirm Early and Persistent Jejunal Crypt Hyperplasia in Poults with Enteritis and Depressed Growth.

Phosphorylated histone 3 (PH3) and cleaved caspase 3 (CCASP3) were used to detect proliferating and apoptotic cells, respectively, in the jejunums of female sibling poults, with and without enteritis and depressed growth, from hatch to day 35. Poults that developed enteritis and depressed growth (SIB flock) were raised on a commercial farm in eastern North Carolina, whereas poults with normal growth and no enteritis (TAU flock) were raised in the Teaching Animal Unit at North Carolina State University College of Veterinary Medicine. Beginning on day 5 through day 35 and at processing, TAU poults were significantly heavier than SIB poults. Jejunal weights, relative jejunal weights, and jejunal densities were greater in SIB poults from day 10 through 35. Jejunal efficiency (body weight /jejunal length) was higher in TAU poults at day 5 and days 10 through 35. Mucosal thickness was greater in SIB poults between days 7 and 21 but greater in TAU poults at days 28 and 35. From day 7 to 35, villus-to-crypt ratios were higher for TAU poults and lower for SIB poults because hyperplastic crypts formed a greater percentage of the mucosa in SIB poults. By day 7, PH3- and CCASP3-positive cells were increased in SIB poults, showing that mucosal changes resulted from combined crypt epithelial hyperplasia and increased apoptosis of villous enterocytes. Findings in this study confirm that enteritis, in the absence of clinical signs, and depressed growth in turkey poults begins by day 7, can be identified microscopically, persists for at least 35 days, is associated with lower processing weights, and has a profound negative effect on turkey growth.

MORBIDITY AND MORTALITY OF WILD TURTLES AT A NORTH CAROLINA WILDLIFE CLINIC: A 10-YEAR RETROSPECTIVE.

The medical records from 1,847 wild turtle patients seen between 2005 and 2014 by the Turtle Rescue Team at the North Carolina State University College of Veterinary Medicine were analyzed. Eastern box turtles (Terrapene carolina carolina; n = 947), yellow-bellied sliders (Trachemys scripta scripta; n = 301), cooters ( Pseudemys spp.; n = 235), common snapping turtles (Chelydra serpentina; n = 165), and eastern painted turtles (Chrysemys picta; n = 93) made up 94.3% of all patients. Patient admissions peaked in May when 25.6% (473/1,847) of all turtles were admitted. Cooters were the most-likely species to be gravid, and the loss of gravid females may put this population at increased risk for decline. The majority of wild turtles presented for anthropogenic causes, primarily vehicular trauma (63.2%; 1,168/1,847), which also had the greatest mortality at 57.8% (675/1,168) of any presenting complaint. Coelomic breach was the presenting injury with greatest risk of dying, increasing the risk of dying by 4.8 times. Other factors that were associated with increased mortality included head injuries, myiasis, and cranial or caudal midline injuries. Of all turtle species, eastern box turtles most commonly presented for nontraumatic conditions including aural abscesses (8.2%; 78/947), upper respiratory infections (6.3%; 60/947), and both conditions concurrently (2.5%; 14/947). While many turtles presented with little to no chance for survival in the wild, 47.6% were eventually released and that number increased to 62.0% released for those that survived 24 hr or longer after presentation. This study adds to the knowledge about the treatment of injured and diseased wild turtles in order to potentially ameliorate the overall impact of humans, especially as a result of vehicular trauma.

A Comprehensive Wellness Program for Veterinary Medical Education: Design and Implementation at North Carolina State University

Research in veterinary medical education has illustrated the challenges students face with respect to mental and emotional wellness, lack of attention to physical health, and limited opportunities to meaningfully engage with persons from different backgrounds. In response, the North Carolina State University College of Veterinary Medicine has adopted a comprehensive wellness program available to all members of the college community. The wellness program is based on a ‘house system’ learning community model and focuses on five broad outcome categories: intellectual growth; mental and emotional health; social distance reduction; cultural competence; and physical health. This case study paper describes the development and implementation of the model at this institution.

CLINICAL, ULTRASONOGRAPHIC, AND ARTHROSCOPIC CHARACTERISTICS OF HAIR SHAFTS WITHIN SYNOVIAL STRUCTURES OF SEVEN HORSES.

To the authors' knowledge, the ultrasonographic appearance of hair shafts within a synovial structure of the horse has not been described previously. The purpose of this descriptive study was to illustrate the clinical, ultrasonographic, and arthroscopic characteristics of confirmed hair shafts within synovial structures of seven horses. A review of medical records of the Farm and Equine Veterinary Medical Center at North Carolina State University College of Veterinary Medicine identified seven horses with clinical signs suspicious for inflammatory or septic synovitis/tenosynovitis that had hair shafts identified within those structures ultrasonographically. Imaging findings were confirmed by surgical arthroscopy, bursoscopy, tenoscopy, or necropsy. Unique ultrasound characteristics of intrasynovial hair included thin, linear, hyperechoic structures, which did not cast acoustic shadows. Findings supported the use of ultrasonography as a complementary diagnostic method for ruling out hair foreign material in horses with signs of inflammatory or septic synovitis/tenosynovitis.

Descriptive Epidemiology for Turtles Admitted to the North Carolina State University College of Veterinary Medicine Turtle Rescue Team

Abstract The North Carolina State University College of Veterinary Medicine Turtle Rescue Team has been treating and releasing wild turtles since 1996 and has compiled a collection of almost 4,000 medical records, now available for consultation by researchers via the North Carolina State University Libraries Special Collections Research Center. Data available for each case include county where turtle was discovered, patient identification number, admission date, species, sex and reproductive status, physical examination findings, clinical diagnosis, last case-entry date, length of stay, and final disposition. Additional data in the records include a day-by-day description of treatment and husbandry performed for each turtle. This report summarizes 2,613 turtle cases examined between 1996 and 2012 by the Turtle Rescue Team, including 12 native species of turtle from 63 North Carolina counties. The sex distribution of those of known sex were evenly distributed. The most common presenting condition was vehicular trauma while garden equipment and fish-equipment–related trauma, pet surrender, and other human-induced injury represented an additional 154 cases. Animal attacks and trauma due to unknown causes were also represented. Other conditions diagnosed on presentation included infection, aural abscessation, nutritional disorder, neurologic disorder, buoyancy disorder, prolapse, and other. A small number of turtles were not diagnosed or were healthy. Ultimate disposition data were available for 2,318 turtles, of which 1,227 were released to the wild. The epidemiological data presented here are similar to information collected in Illinois, Tennessee, and Virginia. Medical records from wildlife hospitals and primary care facilities represent an important opportunity to gain valuable insight into the epidemiology of human interaction with native wildlife species.

The Path of Least Resistance: Using Available Tools to Support the E-Resources Lifecycle

On the surface, it might appear that libraries with different collections, staffing levels, and budgets would have to make use of different tools to address their e-resource management needs. However, in their review of electronic resources lifecycle work at North Carolina State University and Georgia Perimeter College, Tessa Minchew and Sofia Slutskaya discovered that this is not always the case. The presenters reviewed the electronic resources lifecycle and three distinct types of tools (content management systems/wikis, Microsoft Access, and Trello) that are being used in different ways to address different lifecycle-related tasks at each of their institutions.