North Brazil Research Articles

Feasibility of dried blood spot for hepatitis C diagnosis in vulnerable subjects and people living in remote areas from Brazil

BackgroundAgile, accessible and cheap diagnosis of hepatitis C virus (HCV) infection is essential to achieve the elimination of this infection, worldwide, as mandated by the World Health Organzation as part of its strategy for 2030. Dried blood spots (DBS) can be an attractive alternative for sample collection among people living in remote areas and vulnerable populations due to the less invasive collection, its biosafety, and storage & transportation of samples at room temperature.DesignThis study aims to estimate the usefulness of dried blood spot samples for the diagnosis and the assessment of HCV infection rates in three different settings in Brazil. Cross-sectional analysis of a sample collection from different populations, aiming to assess the performance of the testing algorithms and respective procedures among different populations with diverse background infection rates.MethodsWe reported the evaluation of DBS as alternative samples for detecting anti-HCV in different groups in real life conditions: (I) Vulnerable subjects living in remote areas of Southeast, North and Northeast Brazil (n = 1464); (II) Beauticians (n = 288); (III) People who use non-injectable drugs (n = 201); (IV) patients referred to outpatient care (n = 275).ResultsGeneral assay accuracy was 99%, with a weighted kappa value of 0.9, showing an excellent performance. Sensitivities ranged from 87.5% to 100.0% between groups and specificities were above 99.2%. A total of 194 individuals had HCV RNA in serum and concordance of anti-HCV detection in DBS was 98.4%.ConclusionsDBS samples could be used for anti-HCV detection in different populations recruited in real life conditions and ambulatory settings, with a high overall sensitivity and specificity.

Genomic Epidemiology Reveals the Circulation of the Chikungunya Virus East/Central/South African Lineage in Tocantins State, North Brazil.

The chikungunya virus (CHIKV) is a mosquito-borne virus of the family Togaviridae transmitted to humans by Aedes spp. mosquitoes. In Brazil, imported cases have been reported since June 2014 through two independent introductions, one caused by Asian Lineage in Oiapoque, Amapá state, North Region, and another caused by East/Central/South African (ECSA) in Feira de Santana, Bahia state, Northeast Region. Moreover, there is still limited information about the genomic epidemiology of the CHIKV from surveillance studies. The Tocantins state, located in Northern Brazil, reported an increase in the number of CHIKV cases at the end of 2021 and the beginning of 2022. Thus, to better understand the dispersion dynamics of this viral pathogen in the state, we generated 27 near-complete CHIKV genome sequences from four cities, obtained from clinical samples. Our results showed that the newly CHIKV genomes from Tocantins belonged to the ECSA lineage. Phylogenetic reconstruction revealed that Tocantins’ strains formed a single well-supported clade, which appear to be closely related to isolates from the Rio Grande do Norte state (Northeast Brazil) and the Rio de Janeiro state (Southeast Brazil), that experienced an explosive ECSA epidemic between 2016–2019. Mutation analyses showed eleven frequent non-synonymous mutations in the structural and non-structural proteins, indicating the autochthonous transmission of the CHIKV in the state. None of the genomes recovered within the Tocantins samples carry the A226V mutation in the E1 protein associated with increased transmission in A. albopictus. The study presented here highlights the importance of continued genomic surveillance to provide information not only on recording mutations along the viral genome but as a molecular surveillance tool to trace virus spread within the country, to predict events of likely occurrence of new infections, and, as such, contribute to an improved public health service.

High irradiance impairs Isoëtes cangae growth

Isoëtes cangae is a critically endangered submerged quillwort endemic from Amazon ferruginous outcrops fields in north Brazil. The species inhabit a single oligotrophic lake with specific light irradiance quality and quantity. We analyzed light irradiance in field conditions to study the relationship between irradiance and species morphological and physiological traits. Specifically, we compared the I. cangae growth and photosynthesis in ex situ cultivation under control (CI – 20 µmol m−2 s−1) and high irradiance (HI – 400 µmol m−2 s−1). Light spectra in natural habitat suggest that I. cangae is a shade-tolerant species and the results in artificial light confirmed the growth impairment under long-term HI. Plants showed photosynthesis inhibition and the loss of older leaves, which were gradually replaced by new leaves. Our results highlight that the proper management of light irradiance is fundamental in ex situ and in situ cultivation for I. cangae conservation.

Do Eddies Connect the Tropical Atlantic Ocean and the Gulf of Mexico?

AbstractConsistent with satellite‐tracked trajectories of drogued drifters, but at odds with Eulerian assessment of satellite‐altimetry measurements of sea‐surface height, we show that North Brazil Currents Rings (NBCRs) are incapable of bypassing the Lesser Antilles as structures that coherently transport material. We arrive at this conclusion by applying geodesic eddy detection on the altimetric data set over nearly its entire extent. While we detect northwestward translating NBCRs that can be classified as coherent Lagrangian eddies, they typically experience strong filamentation and complete loss of coherence prior to reaching the Lesser Antilles. Moreover, the filamented material hardly penetrates into the Caribbean Sea, let alone the Gulf of Mexico, and not without substantively mixing with the ambient fluid east of the archipelago. The nature of the inability of the de‐facto oceanographic Eulerian, streamline‐based eddy detection technique to produce a correct assessment of the connectivity between the tropical Atlantic and the Gulf of Mexico is rooted in its lack of objectivity.

Sources and Pathways of Intraseasonal Meridional Kinetic Energy in the Equatorial Atlantic Ocean

Abstract In the equatorial Atlantic Ocean, meridional velocity variability exhibits a pronounced peak on intraseasonal time scales whereas zonal velocity dominantly varies on seasonal to interannual time scales. We focus on the intraseasonal meridional velocity variability away from the near-surface layer, its source regions, and its pathways into the deep ocean. This deep intraseasonal velocity variability plays a key role in equatorial dynamics as it is an important energy source for the deep equatorial circulation. The results are based on the output of a high-resolution ocean model revealing intraseasonal energy levels along the equator at all depths that are in good agreement with shipboard and moored velocity data. Spectral analyses reveal a pronounced signal of intraseasonal Yanai waves with westward phase velocities and zonal wavelengths longer than 450 km. Different sources and characteristics of these Yanai waves are identified: near the surface between 40° and 10°W, low-baroclinic-mode Yanai waves with periods of around 30 days are excited. These waves have a strong seasonal cycle with a maximum in August. High-frequency Yanai waves (10–20-day period) are excited at the surface east of 10°W. In the region between the North Brazil Current and the Equatorial Undercurrent, high-baroclinic-mode Yanai waves with periods between 30 and 40 days are generated. Yanai waves with longer periods (40–80 days) are shed from the deep western boundary current. The Yanai wave energy is carried along beams eastward and downward, thus explaining differences in strength, structure, and periodicity of the meridional intraseasonal variability in the equatorial Atlantic Ocean. Significance Statement Past studies show that intraseasonal meridional kinetic energy is important for the deep equatorial circulation (DEC). However, numerical studies use intraseasonal variability with varying characteristics to investigate the formation and maintenance of the DEC. This is partly because of sparse observations at depth that are limited to single locations. This study investigates intraseasonal meridional kinetic energy in the equatorial Atlantic in a high-resolution ocean model that is tested against available shipboard and moored observations. We analyze the spatial and temporal distribution and the baroclinic structure of intraseasonal variability. Using the model, we identify different sources and pathways of intraseasonal energy in the deep equatorial Atlantic. We offer groundwork for further studies on the formation and maintenance of the DEC.

Genetic parameters and trends of growth traits in Nelore cattle raised in the Northern region of Brazil

The objective of this study was to estimate (co)variances and genetic parameters and to predict genetic trends for weight at 120 (W120), 210 (W210), 365 (W365), and 450 (W450) days of age in Nelore cattle raised in the northern region of Brazil. The database comprised records of 30,387 animals born between 2000 and 2013 in the Brazilian North. Estimates were calculated by the Restricted Maximum Likelihood (REML) method, in single- and multi-trait analyses in an animal model. Heritability as obtained using single- and multi-trait models for W120 (0.22 and 0.31), W210 (0.20 and 0.34), W365 (0.51 and 0.51), and W450 (0.49 and 0.51) indicated moderate to high magnitudes, with the possibility of genetic selection and incorporation into the herd. Genetic correlations between growth traits were favorable, ranging from 0.78 to 0.96. Genetic trends for W120 and W210 varied largely, from -0.31 to 4.68 and -0.53 to 7.62 kg/year, respectively. Smaller fluctuations were observed in genetic trends for W365 and W450, which ranged from -1.08 to 10.90 and -1.29 to 12.51 kg/year, respectively. Selection for W365 and W450 proved to be the criterion of choice for Nelore herds raised in the region; however, it may compromise adult performance because of higher costs and time for production. A thorough analysis of mattings is recommended to allow the selection of earlier-developing animals.

Brazilian marine phylogeography: A literature synthesis and analysis of barriers.

In the last 30 years a plethora of phylogeography studies have been published targeting Brazilian marine species. To date, several historical and extant physical and ecological processes have been identified as drivers of allopatric, sympatric and parapatric population genetic differentiation detected along the Brazilian coast. Examples of extant physical barriers include the split of the South Equatorial Current into the Brazil and North Brazil boundary currents, the mouth of major rivers (e.g., Amazon, São Francisco and Doce rivers) and coastal upwellings. Examples of historical barriers include the Vitória-Trindade seamount chain promoting genetic differentiation during periods of glacial maxima and lower sea levels. Examples of ecological speciation include adaptations to different substrata, resource use and reproductive biology. We used published data to build data sets and generalized additive models to identify patterns of spatial phylogeographical concordance across multiple taxa and markers. Our results identify Cape São Roque as the most dominant extant barrier to gene flow along the Brazilian coast, followed by the Vitória-Trindade seamount chain and Cape Santa Marta. Cape Santa Marta is the northern winter limit of the Rio da Plata plume and is intermittently influenced by the Malvinas Current. This study provides a novel explicit quantitative approach to comparative phylogeography that recognizes four Brazilian phylogeographical regions delimited by processes associated with barriers to gene flow.

Influence of the Cape Angle and Flow Speed on Eddy Formation and Detachment: Experimental Results and Comparison with North Brazil Current Eddies

Influence of the Cape Angle and Flow Speed on Eddy Formation and Detachment: Experimental Results and Comparison with North Brazil Current Eddies

Paleomagnetic Constraints From 925 Ma Mafic Dykes in North China and Brazil: Implications for the Paleogeography of Rodinia

AbstractCoeval Neoproterozoic mafic dykes with a well‐constrained isotopic age of 925 Ma are distributed in the North China Craton (NCC) of China and São Francisco Craton (SFC) of Brazil. Several recent studies favor the hypothesis that these two cratons were connected during 925 Ma and constituted building cratons for the supercontinent Rodinia. However, the paleo‐positions of the two cratons in Rodinia have not yet been paleomagnetically resolved. This paper presents the paleomagnetic results of these dykes in both NCC and SFC. Detailed thermal and alternating‐field demagnetization revealed that these dykes record stable components of magnetization with unblocking temperatures below the Curie temperature of magnetite. Rock magnetic results and scanning electron microscope analysis further confirm that the major magnetic minerals in dyke samples are pseudo‐single domain titanomagnetites. The mean directions of the characteristic remanent magnetization (ChRM) of the dykes are distinct from those of rocks of different ages in the sampling localities, and are supported by positive baked contact tests, indicating primary remanent magnetization. The ChRM of the SFC dykes also exhibits dual polarity. Our results suggest that the NCC and SFC were in the moderate to high paleolatitudes of the Southern Hemisphere during approximately 925 Ma, suggesting a possible paleogeographic connection between the two cratons. A new paleogeographic reconstruction of both cratons during 925 Ma is proposed, which is supported by other geological evidence.

Forcing mechanisms of the circulation on the Brazilian Equatorial Shelf

The Brazilian Equatorial Shelf (BES) is a very dynamic region due to the influence of various forcings: trade winds, macrotides, the Amazon River, and the North Brazil Current (NBC). To investigate each forcing’s role in BES circulation, a control simulation and three sensitivity simulations (without rivers, without tides, and without winds) were performed using the Regional Ocean Modeling System (ROMS) with a horizontal resolution of 1/24° and realistic forcings. The results showed that the NBC advected low-salinity waters from the Amazon River northwestward to Caribbean Sea and occasionally eastward through the NBC retroflection. The NBC exhibited the strongest flow when all forcings were included in the simulation. On the other hand, southeasterly winds inhibited the NBC retroflection system and, thus reducing its contribution to North Equatorial Counter Current (NECC) and Equatorial Undercurrent (EUC). The simulation without tides showed a plume more advected by the NBC and constricted to the first 10 m of the water column, while in the simulation without wind the plume was less advected northwestward by the weakened NBC and spread more toward the shelf break. The correlation analysis highlighted the complexity of the system, the complementarity of the forcings and their time-variable interdependence. The three sensitivity simulations and correlation analysis demonstrated the wind to be the main mechanism of the circulation in the BES. The sensitivity simulations also gave insight of how circulation would respond in a future scenario if climate changes persist. Previous studies suggest that the reduced Amazon runoff may indirectly weaken Atlantic ITCZ and thus trade winds causing a weakened circulation in tropical Atlantic. Our results show that this weakened circulation would advect even less fresh water northward, worsening the disturbances in North Atlantic due to the reduced freshwater supply.

Vitamin E deficiency in childhood: a narrative review.

Vitamin E is an important nutrient from the earliest stages of life. It plays key roles as an antioxidant and in the maintenance of the immune system, among others. Vitamin E deficiency (VED), which occurs more frequently in children, is rarely addressed in the literature. This narrative review aims to summarise the chemistry, biology, serum indicators and clinical trials that have evaluated the impact of fortification and other relevant aspects of vitamin E, in addition to the prevalence of its deficiency, in children worldwide. Vitamin E intake in recommended amounts is essential for this nutrient to perform its functions in the body. Serum α-tocopherol is the most widely used biochemical indicator to assess the prevalence of VED. VED has been associated with symptoms secondary to fat malabsorption and may lead to peripheral neuropathy and increased erythrocyte haemolysis. Reduced concentrations of α-tocopherol may be caused by the combination of diets with low amounts of vitamin E and inadequate consumption of fats, proteins and calories. The lowest prevalence of VED was found in Asia and the highest in North America and Brazil. High proportions of VED provide evidence that this nutritional deficiency is a public health problem in children and still little addressed in the international scientific literature. The planning, evaluation and implementation of health policies aimed at combatting VED in the paediatric population are extremely important.

Molecular Epidemiology of Sporadic and Outbreak-Related Salmonella Typhi Isolates in the Brazilian North Region: A Retrospective Analysis from 1995 to 2013.

Typhoidal salmonellosis is a global public health problem occurring in developing endemic regions. In Brazil, cases are mostly registered in the North and Northeast regions. Molecular characterization of the strains is important to understand the epidemiology of disease infections and to design control strategies. The present study retrospectively evaluates the genotyping features of sporadic and outbreak-related Salmonella Typhi isolates from the Brazilian North region. Bacterial isolates were recovered from blood and a rectal swab of patients in the states of Acre and Pará, Brazilian North region, in the period of 1995 to 2013, and were submitted to genotyping by applying Multilocus sequence typing (MLST) and Pulsed Field Gel Electrophoresis (PFGE) reference methods. MLST genotyping revealed the presence of epidemic clones ST1 and ST2, and 20 pulsotypes were identified by PFGE, including four distinct clusters (A–D), and six subclusters (A1–D1) with indistinguishable strains in different periods and locations. To conclude, the obtained data demonstrates the temporal stability, adaptation, and transmission of outbreak-related and sporadic S. Typhi strains over time, contributing to the transmission chain in the region.

Contrasting Sea‐Air CO2 Exchanges in the Western Tropical Atlantic Ocean

AbstractThe western Tropical Atlantic Ocean is a biogeochemically complex region due to the structure of the surface current system and the large freshwater input from the Amazon River coupled with the dynamics of precipitation. Such features make it difficult to understand the dynamics of the carbon cycle, leading to contrasting estimates in sea‐air CO2 exchanges in this region. Here, we demonstrate that these contrasting estimates occur because the western Tropical Atlantic Ocean can be split into three distinct sub‐regions in terms of the sea‐air CO2 exchanges. The sub‐region under the North Brazil Current domain acts as a weak annual CO2 source to the atmosphere, with low interannual variability. The sub‐region under the North Equatorial Current influence acts as an annual CO2 sink, with great temporal variability. The third sub‐region under the Amazon River plume influence shows greater interannual variability of CO2 exchanges, but it always acts as a net oceanic sink for CO2. Despite this large spatial variability, the entire region acts as a net annual CO2 sink of −1.6 ± 1.0 mmol m−2 day−1. Importantly, the Amazon River plume waters drive 87% of the CO2 uptake in the western Tropical Atlantic Ocean. In addition, we found a significant increasing trend in sea surface CO2 partial pressure in the North Brazil Current and North Equatorial Current waters. Such trends are more pronounced than the increase in atmospheric CO2 partial pressure, revealing the sensitivity of carbon dynamics in these sub‐regions to global climate change.

Generation of Vorticity Near Topography: Anticyclones in the Caribbean Sea

AbstractMesoscale anticyclonic eddies dominate the sea‐surface height variability in the Caribbean Sea. Although it is well established that these anticyclones are formed near the eastern boundary of the Caribbean Sea, which is demarcated by the Lesser Antilles, the source of their anticyclonic vorticity remains unclear. To gain insight into this source, we analyze the fluxes of vorticity into the Caribbean at its eastern boundary using a high‐resolution numerical model. We find that the anticyclonic vorticity in the eastern Caribbean Sea predominantly originates from regions where intense ocean currents flow close to the Lesser Antilles. More specifically, St. Lucia and Grenada are hotspots for vorticity generation. The local generation rate scales with the amplitude of the volume transport through the passages between these islands. This finding is in contrast with the view that anticyclonic North Brazil Current (NBC) rings in the Atlantic Ocean are the main source of anticyclonic vorticity in the eastern Caribbean Sea. Our analyses reveal that the direct contribution of the vorticity of the NBC rings is of lesser importance than the local generation. However, the collision of upstream NBC rings with the Lesser Antilles increases the volume transport through the passages into the Caribbean Sea, so that their presence indirectly leads to enhanced local production of anticyclonic vorticity. This process is an example of the importance of vorticity generation near topography, which is ubiquitous in the oceans, and expected to be important whenever currents and steep topography meet.

Controlling Parameters of Benthic Deep-Sea Foraminiferal Biogeography at the Brazilian Continental Margin (11-22°S)

This study presents new quantitative data on benthic foraminifera from three bathymetric transects of the Brazil (11-14°S, 420-1900 m) and Campos (22°S, 430-2000 m) basins. The quantity and quality of organic matter flux as well as substrate properties and hydrodynamic conditions at the sediment-water interface are identified as key parameters controlling assemblage distribution. Based on the total (stained and unstained) fauna, a distinct biogeographic divide between a Globocassidulina subglobosa/crassa assemblage in the Campos Basin and a rosalinid/bolivinid assemblage in the Brazil Basin occurs across the bifurcation of the South Atlantic Central Water into its southward subtropical and northward tropical branches. In the Campos Basin, coarser sediments, increased bottom current activity, and variable nutrient supply favor an assemblage of Globocassidulina subglobosa/crassa, Nuttalides umbonifer and Alabaminella weddellensis. Occurrences of cold-water coral mounds in 870 m provide an ecological niche favoring species such as Alabaminella weddellensis which benefit from trapped nutrients. The Brazil Basin is characterized by increased abundances of Rosalina and Bolivina, while Globocassidulina subglobosa/crassa is comparatively less frequent. Assemblages with G. subglobosa/crassa, Rosalina spp., Bolivina variabilis and Bolivina subreticulata are favored by a relatively high nutrient input at 14°S. Further north, assemblages with Bolivina subreticulata, Bolivina variabilis, Epistominella exigua, G. subglobosa/crassa are located beneath the velocity core of the North Brazil Undercurrent (NBUC), coinciding with more clayey sediments rich in TOC. Occurrences of delicate branching forms such as Saccorhiza ramosa indicate a more stable setting, distal to the main current. Rose Bengal stained (living) specimens are scarce in all three regions, as is typical for deep-sea foraminiferal faunas. Their patterns of species distribution largely reflect those observed for the total fauna.

Volume and Heat Transports by North Brazil Current Rings

A methodology that combines a 24-year long (January 1993 to December 2016) global dataset of eddy trajectories, derived from altimetry, with vertical temperature and salinity profiles from the EN4.2.0 database, derived from XBTs/MBTs, CTDs and Argo floats, was used to reconstruct the mean vertical structure of North Brazil Current (NBC) rings, and to calculate some of their properties. The number of NBC rings formed each year varied from 2 to 8, with an annual-mean formation of 5.3 ± 1.5. During the analyzed period, 112 rings were sampled at least once, at various distances from the center of the rings, leading to a total of 1323 (604) temperature (salinity) profiles available to compute the mean NBC ring, depicting a large, surface intensified, and relatively shallow ring, with intense temperature and salinity anomalies. The meridional volume transport was estimated in 1.3 Sv (1Sv = 106 m3s-1) per ring, leading to an annualized transport of ~7 ± 2 Sv. The amount of South Atlantic Water (SAW) within the mean ring was estimated in ~40 to 60% of the ring volume. According to these estimates, NBC rings may be responsible in different years for approximately 20 to 80% of the northward volume transport associated with the upper limb of the Atlantic Meridional Overturning Circulation (AMOC), and approximately 15 to 55% of the meridional heat transport in the tropical Atlantic.

Anti-Sporothrix Antibody Detection in Domestic Cats as an Indicator of a Possible New Occurrence Area for Sporotrichosis in North Brazil.

Feline sporotrichosis has emerged as an important public health issue in some countries, especially Brazil. Currently, zoonotic transmission of Sporothrix brasiliensis by domestic cats is the major sporotrichosis spread form throughout this country. Sporotrichosis in Brazil is a good model for the One Health concept application, which connects the environment, human and animal health. Under this thinking, the aim of this study was to investigate the seroprevalence of sporotrichosis in cats from Rolim de Moura, Rondônia, Brazil, using antibody detection by an ELISA test previously validated for human diagnosis. For the standardization of this test, 30 serum samples from cats with proven sporotrichosis and 11 sera from healthy cats were used. The assay showed 87% sensitivity and 100% specificity for the diagnosis of feline sporotrichosis. After the standardization, 202 serum samples from distinct cats from Rolim de Moura were evaluated. The test was positive in 63 (31.19%) cats from the studied area. A multivariate analysis revealed that living far from forest or agricultural areas as well as pure breed animals had higher odds ratios (3.157 and 2.281, respectively) for the presence of detectable levels of anti-Sporothrix antibodies. These results show the applicability of this assay in the detection of anti-Sporothrix antibodies in feline serum samples and point to a putative new occurrence area of urban sporotrichosis dispersing to the North region of Brazil.

Chikungunya Virus Asian Lineage Infection in the Amazon Region Is Maintained by Asiatic and Caribbean-Introduced Variants.

The simultaneous transmission of two lineages of the chikungunya virus (CHIKV) was discovered after the pathogen’s initial arrival in Brazil. In Oiapoque (Amapá state, north Brazil), the Asian lineage (CHIKV-Asian) was discovered, while in Bahia state, the East-Central-South-African lineage (CHIKV-ECSA) was discovered (northeast Brazil). Since then, the CHIKV-Asian lineage has been restricted to the Amazon region (mostly in the state of Amapá), whereas the ECSA lineage has expanded across the country. Despite the fact that the Asian lineage was already present in the Amazon region, the ECSA lineage brought from the northeast caused a large outbreak in the Amazonian state of Roraima (north Brazil) in 2017. Here, CHIKV spread in the Amazon region was studied by a Zika–Dengue–Chikungunya PCR assay in 824 serum samples collected between 2013 and 2016 from individuals with symptoms of viral infection in the Amapá state. We found 11 samples positive for CHIKV-Asian, and, from these samples, we were able to retrieve 10 full-length viral genomes. A comprehensive phylogenetic study revealed that nine CHIKV sequences came from a local transmission cluster related to Caribbean strains, whereas one sequence was related to sequences from the Philippines. These findings imply that CHIKV spread in different ways in Roraima and Amapá, despite the fact that both states had similar climatic circumstances and mosquito vector frequencies.

Distribution and provenance of heavy minerals from recent sediments of Green Lake, North Brazil, revisited

Geostatistical and multivariate statistical analyses were applied to heavy mineral data from an Amazonian fluvial-lake system near the Tapajós River mouth to investigate the spatial distribution and source-area of sediments. Twenty-one points were investigated, and the physical characteristics of the Green Lake deepest point were determined. Sand accumulates in the lake margins and mud quantity increases towards the lake center. Heavy mineral assemblage is composed of zircon, tourmaline, kyanite, rutile, staurolite, anatase, sillimanite, garnet, and spinel. Tourmaline, staurolite, and spinel are more abundant in the southeast area of the lake, while kyanite is dominant in the north area and zircon is in the whole lake except in its southeast area. Zircon - tourmaline and zircon - staurolite pairs are negatively correlated (r= -0.947 and -0.775, respectively), while tourmaline - staurolite and sillimanite - anatase pairs have a positive correlation (r= 0.628 and 0.675, respectively) which indicate different source rock types. Geostatistical analysis grouped the heavy minerals in three grups: Group 1 (tourmaline – staurolite – spinel - kyanite) and Group 2 (garnet – rutile – sillimanite - anatase) related to metamorphic source rocks ranging from medium to high grade, and Group 3 (zircon) related to acid igneous source rocks. The heavy mineral assemblage of Green Lake is analogous to the assemblage of the Alter do Chão Formation, indicating that this formation is the source of sediments of Green Lake.

Wintertime process study of the North Brazil Current rings reveals the region as a larger sink for CO<sub>2</sub> than expected

Abstract. The key processes driving the air–sea CO2 fluxes in the western tropical Atlantic (WTA) in winter are poorly known. WTA is a highly dynamic oceanic region, expected to have a dominant role in the variability in CO2 air–sea fluxes. In early 2020 (February), this region was the site of a large in situ survey and studied in wider context through satellite measurements. The North Brazil Current (NBC) flows northward along the coast of South America, retroflects close to 8∘ N and pinches off the world's largest eddies, the NBC rings. The rings are formed to the north of the Amazon River mouth when freshwater discharge is still significant in winter (a time period of relatively low run-off). We show that in February 2020, the region (5–16∘ N, 50–59∘ W) is a CO2 sink from the atmosphere to the ocean (−1.7 Tg C per month), a factor of 10 greater than previously estimated. The spatial distribution of CO2 fugacity is strongly influenced by eddies south of 12∘ N. During the campaign, a nutrient-rich freshwater plume from the Amazon River is entrained by a ring from the shelf up to 12∘ N leading to high phytoplankton concentration and significant carbon drawdown (∼20 % of the total sink). In trapping equatorial waters, NBC rings are a small source of CO2. The less variable North Atlantic subtropical water extends from 12∘ N northward and represents ∼60 % of the total sink due to the lower temperature associated with winter cooling and strong winds. Our results, in identifying the key processes influencing the air–sea CO2 flux in the WTA, highlight the role of eddy interactions with the Amazon River plume. It sheds light on how a lack of data impeded a correct assessment of the flux in the past, as well as on the necessity of taking into account features at meso- and small scales.