Generalized frequency division multiplexing (GFDM) is one of the candidate waveforms beyond 5G for wireless communication. Channel estimation is challenging in wireless transmission because of inherent interference. It is based on a pilot signal that uses Least Square (LS) and Normalized Least Mean Square (NLMS) to perform the estimation. This paper used the Artificial Neural Network (ANN) as a channel estimation method, considered a novel estimation process for the GFDM transceiver. The channel estimation based on ANN depends on the data set generated from LS estimation, which considers the proposed method is optimized to LS based on the backpropagation neural network. The ANN algorithm considered a fitness function to estimate the channel. Levenberg-Marquardt backpropagation (LM), Bayesian Regularization backpropagation (BR), and Scaled Conjugate Gradient backpropagation (SCG) training methods training by using the Matlab toolbox used to perform the estimation. BR training method gives the best performance than LS and other training methods at 22 neurons in hidden layers, which at 20dB give BER = 0.0369 that enhanced over LS by 0.08.