Normal-phase Solvent Research Articles

The application of normal phase liquid chromatography/mass spectrometry by using coaxial continuous flow fast atom bombardment

This article describes the interfacing of a normal phase fused silica capillary high performance liquid chromatography system to a magnetic sector mass spectrometer by using continuous flow fast atom bombardment (CFFAB). While the performance of CFFAB using reversed phase techniques is well understood, there is very little if any documentation on interfacing nonaqueous normal phase systems with CFFAB. This article describes the use of packed fused silica capillary liquid chromatography columns and the corresponding normal phase solvent systems. The experimental parameters required with nonaqueous solvent systems differ significantly from those of aqueous solvent systems. Ditallowdimethylammonium chloride (DTDMAC), a cationic surfactant commonly used as the active ingredient in fabric softener products, was chosen as a model compound to demonstrate the technique. DTDMAC was identified in a commercially available fabric softener product by using on-line normal phase liquid chromatography/mass spectrometry with accurate mass and tandem mass spectrometry.

A Simple, Sensitive Assay for the Spermicide Nonoxynol-9 in Biological Fluids by High-Performance Liquid Chromatography

A new high-performance liquid chromatographic technique has been developed to quantltate nonoxynol-9 in serum, urine, and vaginal fluid. The method is rapid, involves minimal sample preparation, and can be used to analyze a large number of biological fluid samples. The assay elutes a single nonoxynol-9 peak with no interfering components. This was accomplished using a 10-μm pelicular packed amine column, a normal-phase solvent system, and fluorescence detection. Nonoxynol-9 levels as low as 0.23 μg/mL in urine can be detected.

Interpretation of normal-phase solvent strength scales based on linear solvation energy relationships using the solvatochromic parameters π, α and

An analysis of normal-phase solvent eluotropic strength (ε0) and solute adsorption energy (S0) was carried out by use of linear salvation

Characterization of organics in tannery effluents by liquid chromatography mass spectrometry

Combined high performance liquid chromatography mass spectrometry using a moving belt interface has been used to study extracts from five tannery and leather finishing industry discharges. Liquid chromatographic separation was performed with a Zorbax CN column using a normal phase solvent system of hexane + methylene chloride + methanol. Twenty different compounds were identified. Binaphthyl sulfone, which could not be gas chromatographed successfully, was one of the major components found in all five tannery extracts.