Normal People Research Articles

UTILIZING CONVOLUTIONAL NEURAL NETWORKS TO COMPREHEND SIGN LANGUAGE AND RECOGNIZE EMOTIONS

The inability to communicate verbally is widely acknowledged as a significant disability. The primary objective of this research is to create a practical system aimed at individuals with hearing impairments, particularly those who depend on sign language as their primary means of communication. This system aims to enable deaf individuals to express themselves, communicate effectively and facilitate understanding of their language which would otherwise be challenging since most people are unfamiliar with sign language. By employing human gesture interpretation and motion capture, this technology can facilitate the translation of sign language into spoken language and vice versa. Despite the existence of various methods to convert sign language into voice, none of them currently provide an entirely intuitive user interface. Our objective is to create a system that not only translates sign language but also integrates a natural user interface, thus enhancing accessibility for individuals who are blind or have visual impairments. This system will achieve this by recognizing facial expressions and effectively conveying emotions behind words assisting visually impaired individuals in expressing themselves more effectively. Hence, in this project, we tentatively aim to build a system that can ease the lives of blind, deaf and dumb people to some extent. Normal communication like normal people do might never be possible for differently abled people but through our project, we try to provide them with a tool that can help them experience normalcy while communicating with normal people.

Co-regulated ceRNA network mediated by circRNA and lncRNA in patients with gouty arthritis

Numerous studies have demonstrated the involvement of messenger RNAs (mRNAs) and non-coding RNAs, including long non-coding RNAs (lncRNA), circular RNAs (circRNAs) and microRNA (miRNAs), in gouty arthritis onset; however, the regulatory mechanism has not yet been elucidated. Here, we applied whole-transcriptome sequencing to identify the differentially expressed circRNAs, lncRNAs, miRNAs and mRNAs between the gout patients and normal people, and constructed co-regulated networks of circRNAs and lncRNAs according to the competitive endogenous RNA (ceRNA) theory for gouty arthritis onset to improve our understanding of the pathogenesis of this disease. The most significant finding of this study is the co-regulated ceRNA network of circRNAs and lncRNAs in gouty arthritis. The circRNA novel_circ_0030384 and the lncRNAs AAMP, TRIM16, PKN1, XLOC_184579 and XLOC_189826 were upstream genes in the co-regulated network. These upstream genes upregulated miR550a-5p and miR550a-3-5p, which downregulated PSME1 and FERMT3 expression. These mRNAs participated in proteasome dynamics, antigen processing and presentation, and platelet activation, which are associated with inflammation in gouty arthritis. In addition, the circRNA and lncRNAs upregulated miR550a-5p, which downregulated GRK2 and OS9 expression. Also, it proved that the down-regulated of PSME1, FERMT3, GRK2 and OS9 can aggravate gouty arthritis in vitro. In summary, these genes mediate inflammation in gouty arthritis through chemokine signaling to regulate neutrophil function.

Apolipoprotein C3-rich low-density lipoprotein (AC3RL) exacerbates cerebral ischaemic stroke injury by increasing BBB leakage via LOX-1

Abstract Background Ischaemic stroke causes the abrupt reduction or cessation of blood flow to the brain results in a cascade of events that can compromise blood-brain barrier (BBB) integrity. We revealed that an increase in apolipoprotein C3 (ApoC3)-rich low-density lipoprotein (AC3RL) is associated with endothelial cell apoptosis and inflammatory responses, indicating that AC3RL is one of the risk factors for cardiovascular events. Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) triggers a cascade of events within endothelial cells that leads to endothelial dysfunction. Purpose We aimed to investigate whether AC3RL affects BBB permeability via the LOX-1, and thus increases brain damage. Methods We collected the whole blood from normal and patients with ischaemic events and separated them by centrifugation. AC3RL were collected using FPLC. We performed middle cerebral artery occlusion (MCAO) surgery to induce ischaemic stroke using B6 (C57BL/6) and LOX-1-/- mice injected with AC3RL. Triphenyl tetrazolium chloride (TTC) and Evans blue stains were accessed to confirm brain injury and BBB permeability. Results FPLC results showed that patients with ischaemic events presented higher levels of AC3RL compared to normal people. TTC stain showed the increased area of ischaemic penumbra damage in B6 mice with AC3RL injection, and LOX-1-/- mice with AC3RL injection were significantly protected. Evans blue dye leakage was observed in the brain tissues of B6 mice with AC3RL injection. LOX-1-/- mice with AC3RL injection revealed a decrease in BBB permeability. The modified neurological severity score (mNSS) method revealed a significant rise in B6 mice with AC3RL injection, which suggested a decline in motor and nervous function. LOX-1-/- mice with AC3RL injection significantly improved in motor and neurological functioning. Conclusions We demonstrate that AC3RL, through its effects on LOX-1, is associated with an increased risk of BBB damage after stroke. Therefore, pharmacological inhibition of AC3RL-LOX-1 interaction will be the main strategy to reduce severe brain damage. Figure 1: (A) AC3RL percentage (%) in individuals. Normal: healthy people, Ischemic event: patients who have experienced an ischemic event. **p < 0.001 vs. Normal group. (B, C) TTC stain of brain tissue after euthanasia to pinpoint the site of brain damage. The white portion of the mice brains indicated the site of the injury. (D, E) Evans blue dye leakage study in mice brains exhibited BBB permeability changes. (F) Assessment of mice's motor and neurological performance using the mNSS. Data are expressed as the mean ± SEM. **p < 0.001, ****p < 0.0001 vs. sham group, ##p < 0.001 vs. B6 I.V. AC3RL group.Graphical abstractFigure 1.

Relationship Between The Compliance of Antiretroviral Therapy and The Occurrence of Underweight Conditions, Calf Circumference, Handgrip Strength, and Triceps Skinfold in HIV/AIDS Patients at RSSA Malang

Background: Human Immunodeficiency Virus or commonly known as HIV is a virus that infects CD4 cells thereby impaired the human immune system. The number of new HIV cases in Indonesia in 2019 was reported to have reached 50,282 cases and tends to increase from year to year. What often happens in people with HIV/AIDS (PLWHA) tends to experience weight loss accompanied by opportunistic infections that do not occur in normal people. Adherence to therapy is the main factor that must be emphasized by PLWHA in order to achieve treatment success. However, ARV therapy adherence rates are reported to be <80%. This number can be a predictor of therapy failure. One indicator of the success of therapy is when there is clinical improvement in the patient's condition, such as no opportunistic infections or weight gain. Aim: The aim of this study was to determine whether there is a relationship between adherence to antiretroviral therapy and underweight conditions in HIV/AIDS patients. Methods: This study used a cross sectional design with an analytical observational nature and the research subjects were patients at RSSA Malang who visited from June to August 2023. Results: The results of the study showed that there was a significant relationship between adherence to taking ARVs and being underweight (p=0.018) and there was no significant relationship between adherence to taking ARVs with calf circumference, handgrip strength, and triceps skinfold. Conclusion: The conclusion of this study is that the more compliant the patient is with ARV therapy, the higher the increase in body mass index and the smaller the possibility of becoming underweight.

Association between Sexual Abuse Experience and Salivary Stress Biomarkers among Transgender and Gender Non-Conforming Individuals in Chennai – A Cross-Sectional Study

Introduction: Gender-nonconforming people undergo unimaginable sexual and physical abuse. This leads to stress and other stress-related disorders which can be easily determined by salivary stress biomarkers. Early life stress has an association with the immune system linked with stress. Transgenders and gender nonconforming people are prone to sexual abuse in early life more compared to normal people. This study aims to know about the association between sexual and physical abuse experience and salivary stress biomarkers in Transgender and gender-nonconforming individuals. Materials and Methods: This study was a cross-sectional epidemiological study. A snowball sampling design was adopted. The study was conducted in various places in Thozhi Shelter for Transgenders, Chetpet, Chennai among Gender non-conforming individuals and Transgenders. This study was conducted in April 2022. MDA and IL-6 levels were determined from their saliva using the passive drool method. Result: Interleukin L 6 and MDA levels were significantly higher in the GNC Group than in the control group. 63.64% of Transgender and gender non-conforming individuals experienced sexual abuse whereas 73% of them have experienced physical abuse in their lifetime. Conclusion: Transgender and gender non-conforming individuals who experienced physical/sexual abuse have increased IL 6 levels and decreased MDA levels in saliva when compared to the non-transgender individuals.

Malaysian Sign Language Detection with Convolutional Neural Network for Disabilities

Sign language is the main form of communication used by deaf people. Most of their activities like speaking and learning involved sign language. In Malaysia, instead of a deaf person, only a few people know sign language because it's not part of the co-curriculum. Hence, normal people need to know sign language so that they can communicate with deaf people effectively. However, learning sign language requires a lot of effort and takes years of study. Therefore, this research tends to initiate the sign language image detection system that utilizes the deep learning model. The initial effort involves the development of an image detection system that can identify the alphabet of sign language from A to Z. There will be 26 alphabets that contribute to 26 classes of image detection using the Convolutional Neural Network. 85 images will be collected for each alphabet and the total data will be distributed into 80% and 20% for training and testing the CNN model, respectively. The result showed that the proposed system produced more than 95% detection accuracy of the alphabet based on finger sign gestures. For future works, the proposed system will be evaluated with a more complex dataset in terms of words.

Longitudinal relationships between Aβ and tau to executive function and memory in cognitively normal older adults

The early accumulation of AD pathology such as Aβ and tau in cognitively normal older people is predictive of cognitive decline, but it has been difficult to dissociate the cognitive effects of these two proteins. Early Aβ and tau target distinct brain regions that have different functional roles. Here, we assessed specific longitudinal pathology-cognition associations in seventy-six cognitively normal older adults from the Berkeley Aging Cohort Study who underwent longitudinal PiB PET, FTP PET, and cognitive assessments. Using linear mixed-effects models to estimate longitudinal changes and residual approach to characterizing cognitive domain-specific associations, we found that Aβ accumulation, especially in frontal/parietal regions, was associated with faster decline in executive function, not memory, whereas tau accumulation, especially in left entorhinal/parahippocampal regions, was associated with faster decline in memory, not executive function, supporting an “Aβ-executive function, tau-memory” double-dissociation in cognitively normal older people. These specific relationships between accumulating pathology and domain-specific cognitive decline may be due to the particular vulnerabilities of the frontal-parietal executive network to Aβ and temporal memory network to tau.

CNN Algorithm with SIFT to Enhance the Arabic Sign Language Recognition

Sign language is used as a primary means of communication by millions of people who suffer from hearing problems. The unhearing people used visual language to interact with each other, Represented in sign language. There are features that the hearing impaired use to understand each other, which are difficult for normal people to understand. Therefore, deaf people will struggle to interact with society. This research aims to introduce a system for recognizing hand gestures in Arabic Sign Language (ArSL) through training the Convolutional Neural Network (CNN) on the images of ArSL gestures launched by the University of Prince Mohammad Bin Fahd, Saudi Arabia. A Scale Invariant Feature Transform (SIFT) algorithm is used for creating the feature vectors that contain shape, finger position, size, center points of palm, and hand margin by extracting the Important features for images of ArSL and transforming them to points of the vector. The accuracy of the proposed system is 97% using the SIFT with CNN, and equal to 94.8% nearly without SIFT. Finally, the proposed system was tried and tested on a group of persons and its effectiveness was proven after considering their observations.

Tourism Adaptation Strategy During the Crisis from Covid-19 Pandemic at Ngoi District, Luang Prabang Province

This study is about " Tourism adaptation strategy during the crisis from Covid-19 pandemic at Ngoi district, Luang Prabang Province" was studied in a qualitative format with an interview questionnaire as a tool for data collection. The results of the study found that the state of tourism in Ngoi City during the spread of the Covid-19 disease in Ngoi District is that tourists have dropped to 99.15%. Ngoi by training personnel and open the reception of tourists in the green tourism area and speed up the implementation of Lao safe tourism standards, most of the businesses that provide tourism services have closed their businesses by turning from the service business to farming and raising animals and starting to reopen in December 2021 and the local people are turning from the service profession to tourism to agriculture. raise animals and collect forest products of Dong, from the analysis of the tourism environment of Ngoi District and found that Ngoi District has strengths that can cope with this crisis situation more than weaknesses and there are also favorable opportunities from the external environment that can restore and develop the tourism situation in Ngoi District to normal people quickly. The use of corrective strategy (WO Strategy), the use of defensive strategy (ST Strategy) and the use of receiving strategy (WT Strategy) so that stakeholders in Ngoi district can adapt to the situation.

Palmitic acid promotes miRNA release from adipocyte exosomes by activating NF-κB/ER stress

ObjectiveThe release of adipose tissue-derived miRNAs is increased under conditions of obesity, but the exact molecular mechanisms involved have not been elucidated. This study investigated whether obesity-induced increases in palmitic acid (PA) content could activate the NF-κB/endoplasmic reticulum stress (ER stress) pathway and promote the expression and release of exosomal miRNAs in adipocytes.MethodsAbdominal adipose tissue and serum samples were collected from normal weight individuals and people with obesity to clarify the correlation of serum PA content with NF-κB/ER stress and the release of exosomal miRNAs. NF-κB and ER stress were blocked in obese mice and in vitro cultured adipocytes to demonstrate the molecular mechanisms by which PA promotes the release of exosomal miRNAs.The morphology, particle size and distribution of the exosomes were observed via transmission electron microscopy and NTA.ResultsAccompanied by increased serum PA levels, the NF-κB/ER stress pathway was activated in the adipose tissue of people with obesity and in high-fat diet (HFD)-induced obese mice; moreover, the levels of miRNAs in both adipose tissue and serum were increased. P-p65 (Bay11-7082) and ER stress (TUDCA) blockers significantly reduced the levels of miRNAs in abdominal adipose tissue and serum, decreased blood glucose levels, and improved glucose tolerance and insulin sensitivity in obese mice. In 3T3-L1 adipocytes, high concentrations of PA activated the NF-κB/ER stress pathway and increased the expression and release of miRNAs in exosomes. P-p65 (Bay11-7082) and ER stress (TUDCA) blockers significantly reversed the increased release exosomal miRNAs cause by PA.ConclusionsObesity-induced increases in PA content increase the expression and release of miRNAs in adipocyte exosomes by activating the NF-κB/ER stress pathway.

Prevalence of Chronic Kidney Disease and Associated Factors among the Diabetic and Prediabetic Population in the Bandare-Kong Cohort Study; A Population-Based Study.

This investigation aims to examine the relationship between diabetes and prediabetes with chronic kidney disease (CKD) while taking into account key risk factors such as gender, age, lifestyle, smoking habits, and blood pressure. Between November 17, 2016, and November 22, 2018, 4063 subjects aged 35 to 70 years were enrolled in the first phase of the Bandare-Kong Non-Communicable Disease (BKNCD) Cohort Study, which is part of the PERSIAN (Prospective Epidemiological Research Studies in IrAN) cohort and was conducted in a coastal region of the Hormozgan province in southern Iran. CKD was calculated using the Modification of Diet in Renal Disease (MDRD) formula based on glomerular filtration rate (GFR)<60 mL/min per 1.73 m2 , or albumin/Cr>30 mg/g in random urine, self-reported kidney failure, or dialysis. Urine albumin and creatinine were determined by standard kits (Pars Azmoon, Tehran, Iran) and the BT1500 automatic chemistry analyzer (Biotecnica Instruments, Rome, Italy). The prevalence of CKD was found to be 15.3%, with 29.6% identified in diabetic individuals and 16.5% in prediabetic patients. So, the prevalence of CKD in diabetics was higher than prediabetics and normal people. Increased age, dysglycemia (diabetes or prediabetes), hypertension, and use of angiotensin receptor blockers were markedly associated with an elevated risk of CKD in adults. The study emphasizes the importance of early detection and management of CKD risk factors, particularly among high-risk individuals, to mitigate CKD progression and associated complications. By addressing modifiable risk factors, proactive screening, and enhanced awareness, significant strides can be made in reducing CKD burden and improving patient outcomes.

Machine Learning-based Categorization of Airbnb Listings in NYC

This research focuses into creating a machine-learning-driven system to categorize Airbnb listings in New York City (NYC) based on neighborhood attributes and listing features. Utilizing data scraped from InsideAirbnb.com, including custom attributes such as median household income, craft beer and specialty coffee counts, and a connectivity score, KMeans clustering was applied to classify listings into four groups. These groups, named Normal People, The 2%, Central Action, and Hip Kids, offer insights into the city’s diverse landscape of Airbnb offerings. The classification model’s accuracy was validated using various semi-supervised learning techniques, resulting in 100% accuracy for some models. Dropping significant features like income in validation tests reduced accuracy to 66-78%, showing the importance of feature selection. The study demonstrates the potential of machine learning in enhancing Airbnb’s understanding of customer preferences and refining inventory management.

Kinematic gait analysis for patients with meniscus injury

To investigate application of the Codamotion 2CX1 three-dimensional dynamic joint motion capture system to analyze the kinematic characteristics of patients with different degrees of meniscus injury. From December 2020 to June 2022, 135 patients with meniscus injury and recruited normal people were collected, including 82 males and 53 females, aged 14 to 29 years old, with disease duration of 1 to 3 months. Combined with clinical symptoms and MRI examination, the diagnosis of meniscus injury was confirmed, and the patients were divided into stages, including 37 cases of grade 0(normal), 30 cases of gradeⅠ, 33 cases of gradeⅡ, 35 cases of grade Ⅲ according to Stoller grading standard. Subjects in each group were tested walking by using Codamotion 2CX1 three-dimensional dynamic joint motion capture system. Quantitative indexes of walking and kinematics were collected, including knee flexion and extension, internal and external rotation and internal and external turning, and their kinematics were analyzed. In the distribution of knee flexion/extension, there were significant differences in maximum knee flexion, minimum knee extension and knee flexion and extension range among 4 groups(P<0.05). In the distribution of internal/external rotation of knee joint, there were significant differences in the range of internal rotation and rotation of knee joint among 4 groups(P<0.05). In the distribution of internal/external turning of knee joint, there were significant differences in the range of internal and external turning of knee joint among 4 groups(P<0.05). The clinical stage progression was positively correlated with the range of motion of knee extension, external rotation, internal and external turning and turning range(P<0.05). It was negatively correlated with knee flexion, internal rotation, flexion extension and rotation range(P<0.05). The internal and external rotation angles of knee joint could be used as independent factors influencing the clinical stage of meniscus injury (P=0.006, 0.019<0.05). The knee movement of patients with meniscus injury has obvious changes, and the changes are different under different clinical stages. Gait analysis provides a reliable basis for the kinematic analysis of meniscus injury, helps to better understand the kinematic indexes of joints, and provides a reliable auxiliary diagnosis and treatment plan, which provides a new direction for the follow-up medical research.

Event-related potentials study on the effects of high neuroticism on senile false memory.

To study the false memory among senile normal people with high neuroticism and low neuroticism using neuropsychological scales and event-related potentials (ERPs), and to explore the effects of high neuroticism on false memory and its neuroelectrophysiological mechanism. A cross-sectional study was conducted, in which the general situation questionnaire, adult version of Eysenck personality questionnaire (EPQ) and Montreal cognitive assessment (MoCA) scale were used to establish a multi-dimensional survey in senile normal people over 60 years old from communities in Zhengzhou, and the EPQ and general situation questionnaire were used to comprehensively screen and divide the study subjects into high neuroticism group and low neuroticism group from 206 senile people. The population was matched by 1:1 according to gender, age (±2 years), and years of education (±2 years), and 40 subjects were finally enrolled for detection of electroencephalograph (EEG) components using ERPs. The Deese-Roediger-McDermott (DRM) paradigm of false memory was designed using E-prime2.0 system, and the stimulus program was presented. The EEG signals of the study subjects were recorded in real time and acquired using 64-channel Neuroscan EEG signals acquisition system. High neuroticism group was evidently lower in the mean accuracy than low neuroticism group, and the difference in the mean accuracy was statistically significant (P = 0.013), but the difference in reaction time was not statistically significant. 2. The mean amplitude of EEG component N400: The difference in the main effect of N400 in the brain region was significantly different (P<0.001), and the mean amplitude of N400 was the largest in frontal region, followed by central region and parietal region successively (all P<0.05). There was no statistically significant difference in the neurotic main effect or the interaction effect of neuroticism and brain region. The latency of N400: There was no significant difference in the neurotic main effect, main effect of the brain region or the interaction effect of neuroticism and brain region. 3. The mean amplitude of EEG component LPC: The difference in the main effect of the brain region was significantly different (P<0.001), and the mean amplitude of LPC was the largest in frontal region, followed by central region and parietal region successively (all P<0.05). There was no significant difference in the neurotic main effect, neuroticism or the interaction effect of neuroticism and brain region. As to the latency of LPC, there was significant difference in the main effect of the brain region (P = 0.025), and the latency of LPC was shorter in frontal region than that in central region (P<0.05). The differences in the neurotic main effect, interaction effect of neuroticism and brain region were not statistically significant. High neuroticism can significantly increase the false memory of senile normal people. The EEG components N400 and LPC are potential early indicators of high neuroticism affecting false memory. High neuroticism may influence false memory by affecting the frontal cortex function.

Influence of Anatomy-Based Fitting in Cochlear Implant Users on Music Perception Using the Montreal Battery of Evaluation of Amusia.

The aim is to investigate the influence of the anatomy-based fitting (ABF) in cochlear implants (CI) on the perception of specific musical features. The Montreal Battery of Evaluation of Amusia (MBEA) test battery was performed with a collective of 16 CI users with and without an anatomy-based fitting and 9 normal hearing subjects. The insertion angles of the intracochlear electrode arrays were calculated using Otoplan® (MED-EL, Innsbruck, Austria) software. CI users with an anatomy-based fitting achieve better results in the MBEA in all categories, especially in rhythm, meter, and memory. There is no effect of insertion depth alone on the ability to perceive musical features, even after anatomy-based fitting. The results of the CI users in comparison to normal hearing people confirm previous studies. Apart from better speech understanding in CI users, which has been demonstrated in other studies, the anatomy-based fitting also contributes to a better perception of musical features. Currently, there are no comparable studies on this relationship. Investigations with further parameters with the help of the MBEA are pending. Our results show that anatomy-based fitting of cochlear implants significantly improves the perception of specific musical attributes and thus leads to an increased enjoyment of music.

(Im)possible Facilitating Environments: the Spaces of Family in Sally Rooney’s &lt;i&gt;Normal People&lt;/i&gt;

Building upon the concept of the “good enough” facilitating environment developed by pediatrician and psychoanalyst D. W. Winnicott, this article aims to analyze the family relationships depicted in the novel Normal People by Sally Rooney. Through the contrast of experiences, the author develops two distinct family environments — for Connell Waldron, the space is nurturing, while for Marianne Sheridan, it is marked by violence. This narrative choice allows for reflections on the impacts of these spaces on the emancipation process of the protagonists. They initiate a romantic relationship in their final year of high school and, amidst ups and downs, remain intertwined in each other's lives until the end of college, when they must navigate separate paths. Through the dialogues between the protagonists and the information provided by the third-person narrator, which alternates between the two points of view, this article aims to analyze how the family dynamics of each character influence their self-perception and the choices they make over the four-year period covered in the novel.

Affinity of endosulfan and HCB with human serum albumin affects serum concentrations in a general population

Endosulfan and hexachlorobenzene (HCB) were previously widely used organochlorine pesticides. Much attention has been paid to trends in human exposure to endosulfan and HCB and to the transport and distribution of endosulfan and HCB within the body. We determined endosulfan I, endosulfan II, and HCB concentrations in serum samples collected from the general population of Wuxi in Jiangsu Province (China) between 2012 and 2016. The endosulfan concentrations varied little throughout the study period, but the HCB concentrations increased. The HCB concentrations were significantly higher in serum from overweight people and smokers than in serum from normal weight people (p = 0.028) and non-smokers (p = 0.011), respectively. HCB binds to human serum albumin (HSA) and can form halogen bonds to CYS-461 and ARG-484 at site II (subdomain IIIA). Fatty acids and nicotine increase the affinity between HCB and HSA, explaining the higher HCB concentrations in serum from overweight people and smokers than normal weight people and non-smokers, respectively.

The immun function of dendritic cells in SFTS patients

ObjectivesSevere fever with thrombocytopenia syndrome (SFTS) is a newly emerging infectious disease caused by a novel bunyavirus in which host immune system suppression is thought to be crucial in disease development. Dendritic cells (DCs) are professional antigen-presenting cells (APCs) critical for initiation and orchestration of the immune response. And it have been suggested that functionally impaired DCs may mediate the suppression of host-specific T-cell immune responses and thus facilitate viral persistence and disease progression.This study was designed to improve the in vitro culture method for DCs and investigate the different immunologic functions of DCs between SFTS patients and healthy people. MethodsAll confirmed SFTS patients (N = 10) were recruited from the Jinan Infectious Diseases Hospital in 2019; routine laboratory parameters were collected. The frequencies, phenotypes were analyzed by flow cytometry. And the levels of 8 cytokines in the cell culture supernatant were detected by flow cytometry. ResultsOn day 8 of the incubation period, cells were harvested and analyzed by flow cytometry. There were significant differences in the rates of CD1a-, CD83-positive cells between SFTS patients and healthy people (all P < 0.05). The detection of 8 cytokines in the culture supernatant showed that the expressions of IFN-α and IFN-γ in the culture supernatant of DC cells in SFTS patients were lower than those in normal people (P < 0.05, P < 0.01). ConclusionsThe present results indicate that DCs may be functionally impaired in SFTS. A decreased level of circulating mDCs was closely correlated with SFTS progression.

The Impact of the Current Political Crisis on Public Life in Pakistan: An Economic Case Study of Recent Events

From the day first 14 Aug 1947 to now Pakistan passed tough time throughout history, Lack of Economic resources and financial instability political turmoil leadership crisis bad governance and many other factors. All these elements put Pakistan on the brink of destruction. According to a cautious survey in 1987 normal people were earning $5.50 per day. The overall percentage was $0.20% earning. The economic progress of the country was $96.80%. This percentage fluctuates over time according to the economic and political conditions of Pakistan. In 2018 it changed to $77.60% and overall entire population earned $1.20%. However on the flip-side population according to the 2000 Census, Pakistan’s population was 160 Million. In 2017 it increased the figure to 220 Million. Thus, overall it can be analyzed economic sources were not enough to meet the country’s requirements. Since April 2022 until now Pakistan’s economic situation are going lower and declining day by day due to political instability, poor governance, sick administration and financial crisis.

Selenium intake is an effective strategy for the improvement of cognitive decline in low cognition older Americans

Age-related cognitive decline is a prominent concern in older adults and selenium (Se) deficiency has been found to be associated with cognitive deficits. For the first time, the present study explored the association between Se intake and cognitive performance in older people with/without cognitive impairment using the data from the National Health and Nutrition Examination Survey 2011–2014. Weighted linear regression models were conducted to evaluate the association between dietary Se/total Se intakes and cognitive assessments. A total of 2387 participants were included. The significant positive association between dietary Se/total Se intakes and total scores of cognitive functioning tests existed only in the older people with low cognitive performance (p < 0.001), not in those with normal cognitive performance. In conclusion, Se intake was beneficial for cognitive decline only in the low cognition older people but failed in normal cognition older people.