The application of timber-based strengthening solutions to existing wooden and masonry structures, combines several benefits, such as reversibility, compatibility, lightness, sustainability, affordability, and effectiveness. With reference to existing timber floors, an efficient method to enhance their seismic response is the fastening of an overlay of plywood panels to the existing sheathing, an intervention that greatly improves in-plane strength, stiffness, and energy dissipation. In order to promote the use of this retrofitting solution in practice, this work presents a set of calculation tools supporting the design and advanced numerical modelling of timber diaphragms strengthened with plywood panels. The suite of tools allows to first estimate the full nonlinear, cyclic in-plane response of the strengthened diaphragms starting from the geometrical and material properties of the existing sheathing and the plywood overlay, as well as the mechanical characteristics of the fasteners. As second step, such estimated in-plane response can be transformed into a constitutive law for performing nonlinear numerical simulations, by means of a user-supplied subroutine developed for finite element software DIANA FEA. The presented calculation examples and the performed validation against reference studies from literature, show that the developed tools can provide an accurate estimate of the in-plane response of the diaphragms, and enable an efficient numerical simulation of their seismic behaviour. The implemented tools can be used to both obtain preliminary indication for plywood-based seismic retrofitting design, and to calibrate the interventions on existing diaphragms based on the specific characteristics and needs of a building, relying on the adaptability and versatility of this strengthening method.