In recent decades, computer vision-based methods have been introduced into engineering structures to provide full-field, non-contact vibration measurement. However, the vision-based vibration measurement method is challenging to measure the targetless structures. This paper proposed a target-free out-of-plane vibration measurement method using projection speckle and camera self-calibration technology. In this method, the camera extrinsic parameter estimation result based on the direct method is used as the initial value in the proposed method. Then, a nonlinear inequality constraint on the extrinsic parameters is established regarding the epipolar condition to minimize the reprojection error. Furthermore, the projection relationship between the projection speckle displacement and the actual displacement of the structure is studied so that the deviation of the out-of-plane vibration displacement is corrected. In the experimental verification, 3D reconstruction and rigid translation experiments are designed, respectively. The results show that the self-calibration accuracy of the binocular camera proposed in this paper is notably improved compared with the existing methods. At the same time, the deviation correction effect of the vibration displacement is significant. Finally, the typical flat plate structure and composite wing structure are taken as vibration measurement cases to demonstrate that the proposed approach is reliable and accurate in measuring the dynamic responses of engineering structures.