Noni Leaf Research Articles

Sunscreen Cream Formulation of Noni Leaf Extract (Morinda citrifolia L.) with Emulsifier Combination of Tween 80 and Lecithin

Background: Noni leaf (Morinda citrifolia L.) extract is a natural product that can be used as a sunscreen. The extract contains flavonoids which function act as an antioxidant. In this work, sunscreen cream formulated with noni leaf extract was prepared using a combination of tween 80 and lecithin. Objective: The purpose of this study is to evaluate how the combination of tween 80 and lecithin affects the physical qualities of the cream, such as organoleptic, homogeneity, emulsion type, spreadability, adhesion, pH, and stability over 28 days of storage at room temperature, and to find the best formula. Methods: This study used 10% of noni leaf extract in the cream formulation. The Simplex Lattice Design (SLD) method was used to determine the effect of different concentrations of the two emulsifiers on the cream's spreadability, adhesion, and pH. Furthermore, the SLD was used to find the best formula. Results: The results showed that different concentrations of the emulsifier, which are the tween 80 and lecithin combination, affected the physical properties and storage stability of cream preparations. The interaction of tween 80 and lecithin is having a significant impact on the cream's adhesion and spreadability; however, the effect of the interaction on the pH value was not significant. Conclusion: The formula containing 2.5 % tween 80 and 2.5 percent lecithin was found to be the most effective in fulfilling the cream physical properties while remaining stable during storage.

Effect of topical ethanolic extract of noni leaf on post-extraction wound by observation of wound diameter and osteoblast cells

Objective: The aim of this study is to determine the effect of noni leaf ethanol extract gel on post extraction wound of rabbit teeth observed from wound diameter and osteoblasts cell count. Tooth extraction is one of the treatments performed in the oral region that cause wound. Application of substance or medicine might help accelerate and improve wound healing.
 Material and Methods: Twenty-four rabbits divided into three groups: negative control, positive control with 10% povidone iodine, and treatment group with 10% ethanol extract gel of noni leaf. The tooth was extracted from the mandibular left incisor. The diameter of the wound area was examined on day 0, 3, 7, and 14 while the osteoblasts cell count was obtained from rabbits’ specimen in each group on 14th day. Measurement of wound diameter was done by using calipers, while the number of osteoblasts were calculated using toluidine blue histochemistry stain and light microscope with 400x magnification.
 Results: The ratio of wound diameter were better in treatment group with significant difference (p=0.048) while osteoblasts cell count were not significantly different among all groups.
 Conclusion: Topical application of noni leaf had an effect in decreasing mucosal wound diameter but there was not any evidence yet that it was increased osteoblast cells on socket wound healing process.

Daya Hambat Ekstrak Daun Mengkudu (Morinda citrifolia L.) terhadap Pertumbuhan Bakteri Bacillus cereus

Noni (Morinda citrifolia L.) is a plant typical of tropical regions including Indonesia, whose fruits, leaves, seeds, and roots are often used in traditional medicine, including as an antibacterial. This research was conducted to determine the inhibition zone of noni leaf extract (Morinda citrifolia L.). An inhibition test was carried out according to the Kirby-Bauer method with 4 replications on the growth of Bacillus cereus. The results of this study showed that a concentration of 25% produced an average inhibition zone of 9.075 mm, a concentration of 50% produced an average inhibition zone of 8.650 mm, a concentration of 75% produced an average inhibition zone of 9.275 mm, a concentration of 100% produced an average inhibition zone of 10,050 mm, positive control using ciprofloxacin produced an average inhibition zone of 30.475 mm and negative control using DMSO produced an average inhibition zone of 0.000 mm. The results of the comparative test using the Kruskal-Wallis statistical test with a p. value of 0.003 (p<0.005) and the results of the Mann-Whitney test showed that there was a significant difference between P2 and P4 with a value of 0.042 (p<0.005). The conclusion obtained from this study was that the noni leaf extract had a weak zone of inhibition on the growth of Bacillus cereus at a concentration of 100% according to Greenwood's criteria.

Uji Daya Antiinflamasi Ekstrak Etanolik Daun Mengkudu (Morinda Citrifolia (L.)) Terhadap Mencit Putih (Mus Musculus) Jantan Galur Swiss Yang Diinduksi Karagenin

Inflammation is the body's response to protect it self from chemicals, infectious agents, and damage to body tissues caused by injury. Flavonoid compounds can be used as anti-inflammatory by inhibiting the activity of cyclooxygenase (COX) and lipoxygenase enzymes. The amount of flavonoid content in noni leaves was 15.97 mg QE/g extract. The difference between this study and the previous research conducted by Rifaldy, et al (2019) lies in the test animals, the method of measuring inflammation, and the dose of extract used. This study aims to determine the anti-inflammatory power and dosage of the ethanolic extract of noni (Morinda citrifolia (L.)) leaves. This study is an experimental study, using a completely randomized design with one-way pattern with one treatment factor, namely variations in the dose of Noni Leaf Ethanolic Extract (EEDM), namely 42 mg/20gBW mice, 56 mg/20gBW mice, and 70 mg/20gBW mice. The anti- inflammatory test method uses artificial edema formation with carrageenin. The research data was in the form of percentage of anti-inflammatory power (%DAI), thenstatistically analyzed using SPSS with 95% confidence level. The results of the anti-inflammatory power of EEDM from the smallest to the largest doses produced anti-inflammatory properties, namely 55.30%, 62.28%, and 69.41%, this means that the larger the dose, the higher the percentage of anti-inflammatory power (p<0.05). The percentage of anti-inflammatory power of EEDM at a dose of 70 mg/20gBW mice had an anti-inflammatory effect that was not significantly different from the positive control (p>0.05).

Bioautographic Analysis And Antibacterial Activity Test Of Mengkudu Leaf (Morinda Citrifolia L.) Ethanol Extract On The Bacteria Propionibacterium Acnes

Noni contains alkaloids, flavonoids, tripertinoid, protein, lime, iron, carotene, glycosides antarkiron. The content of anthraquinin, scaloptin, flavonoid and adaptogen compounds are active compounds that have antifungal and antimicrobial effects. The antibiotics commonly used are antibiotics such as tetracycline, clindamycin and others. These chemical-based antibacterials have many negative side effects, therefore other alternatives are needed as antibacterials, one of which is by using noni. This study aims to analyze bioautography and determine the antibacterial activity of noni leaf ethanol extract against Propionibacterium acnes bacteria.This research was conducted experimentally using noni leaf ethanol extract with concentrations of 30%, 40%, 50% as independent variables and bioautographic analysis and antibacterial activity as dependent variables. The bioautography analysis was performed using TLC and the antibacterial activity test was performed using paper discs.The results of antibacterial testing of noni leaf ethanol extract with a concentration of 30% had an inhibition zone of 8.36 mm with a medium category, a concentration of 40% had an inhibition zone of 10.3 mm with a strong category and a concentration of 50% had an inhibition zone of 11.1 mm in a strong category. positive control tetracycline 30µg had an inhibition zone of 20.8 mm which was categorized as very strong. The results of the bioautography test using contact bioautography using the mobile phase of chloroform: n-hexane (7:3). The inhibition zone was obtained at an Rf value of 0.17, and the characteristics of the chromatogram spots were carried out by spraying 10% FeCl3 suspected that the spots were flavonoid compounds.

Toksisitas Daun Mengkudu (Morinda citrifolia L.) sebagai Mat Elektrik terhadap Kematian Nyamuk Aedes aegypti

Dengue Hemorrhagic Fever (DHF) in Indonesia has increased from year to year. Community usually used chemical insecticides to control dengue fever. However, the continuous use of chemical insecticides will have a negative impact on the environment and humans and the possibility of resistance to mosquitoes. An alternative that can be done is to use a bioinsecticide made from noni leaves (Morinda Citrifolia L.) which contains flavonoids, saponins and alkaloids. This study aimed to analyze the toxicity of noni leaf (Morinda citrifolia L.) as an electric mat to the death of the Ae. aegypti. The type of research is a quasi-experimental study with a posttest only research design with a control group design using 4 variations of 1 gram, 1.5 gram, 2 gram, 2.5 gram with five repetitions. The samples used were 25 Ae. aegypti mosquitoes. Statistical analysis used is One Way Anova Test and Probit Test. The results of the average percentage of deaths during 24 hours of observation were 30%, 41%, 54% and 68%. One Way Anova test showed the value (p-valuee=0.000). The probit test showed the LC50 of 1.735 gram/6m3. The conclusion of this research is noni leaf has toxicity to kill the Ae. aegypti mosquito. However, its effectiveness is still below the WHO requirements because it has not reached 90%.

ANALISIS BIOAUTOGRAFI DAN UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN MENGKUDU (Morinda citrifolia L.) TERHADAP BAKTERI Propionibacterium acne

Noni or (Morinda citrifolia L.) contains active anthraquinone, scaloptin, flavonoid, and adaptogen compounds that have antifungal and antimicrobial effects. Antibacterial chemicals have many negative side effects, and other alternatives are needed as antibacterials, one of which is by using noni. This study aims to analyze bioautography and determine the antibacterial activity of noni leaf ethanol extract against Propionibacterium acne bacteria.

ANTIPYRETIC EFFECTIVENESS OF METHANOL EXTRACT OF NONI (Morinda citrifolia L.) LEAVES ON INDUCED BREWER’S YEAST

Fever is an increase in body temperature above the normal body temperature (36°-37° C). Fever is overcome by the administration of antipyretic supply (paracetamol), can also use tradisional medicines from medicinal plants such as noni leaves. This research aims to find out the antipyretic effect of methanol extract of noni leaves (Morinda citrifolia L.) on white mice in the induction of Brewer's Yeast. The extract was prepared by maseration method using methanol as a solvent. Mice were divided in 5 treatment groups. Each mice was induced with Brewer's Yeast at a dose of 112 mg / 20 grams of body weight for mice. The first group in the CMC given 1% as negative control, the second group was given Paracetamol as a positive control, the 3rd, 4th and 5th were given noni leaf extract at a dose of 50 mg / KgBB, a dose of 150 mg / KgBB, a dose of 250 mg / KgBB. Temperature measurement were carried out for 3 hours at intervals of 30 minutes. Measurement data were analyzed statistically using ANOVA test (Analisys Of Variance) with Anova one way method. The results of the research were obtained, that methanol extract of noni leaves can reduce the temperature of fever. The best dosage as an antipyretic is a dose of 250 mg / KgBB. Key words:Antipiretic, methanol extract, Noni leaf(Morinda citrifolia L), Brewer's Yeast

Increasing antioxidant activity of soursop (Annona muricata L.) and noni (Morinda citrifolia L.) leaves fermented by Lactobacillus plantarum BP102

Free radicals are constantly produced by either cell metabolism or from external sources. At high concentration, they induced a tissue damage called oxidative stress. Soursop leaf (Annona muricata L.) and noni leaf (Morinda citrifolia L.) are medicinal plants with potency as antioxidants. This study aimed to evaluate the capacity of Lactobacillus plantarum BP102 in elevating the antioxidant activity of soursop and noni leaves. Dried-powder and methanol extract of soursop and noni leaves were diluted with sterile distilled water 3 g/30 mL and 0.3 g/30 mL, respectively, inoculated with 1% (v/v) of L. plantarum BP102 inoculum. The antioxidant activity was carried out using the 2.2-diphenyl-1-picrylhydrazyl (DPPH) method. The antioxidant activity increased in dried-powder and methanol extract of soursop and noni leaves with different activity levels after being fermented using L. plantarum BP102 based on IC50. The increasing antioxidant activity in dried-powder of soursop leaves IC50 6.41±0.06 to 0.034±0.01 mg/mL (99.5%) was higher than of the methanol extract IC50 2.78±0.00 to 0.11±0.01 mg/mL (96%). Unfortunately, the effect of fermentation towards noni leaves could only be observed in the form of methanol extract IC50 12.8±0.01 to 0.33±0.02 mg/mL (increased by 97.4%), the dried-powder of noni leaves was suspended and produced a dark color. The probiotic L. plantarum BP102 was used as a fermented agent in increasing the bioactive compounds especially related to antioxidant activity.

Quality Of Duck Eggs At Different Soaking Times In Noni Leaf Extract Stored For 15 Days

Duck eggs are one type of protein source food that is much favored by the people of Indonesia. However, currently there are not many ways of preservation to maintain the quality of duck eggs in storage. With preservation by immersion with noni leaf extract, it can be an alternative in maintaining the quality of duck eggs in storage for 15 days. This is because noni leaves contain elements of chemical compounds such as amino acids, phenolic compounds, ursulic acid, alkaloids, phenols, and glycosides which are antimicrobial, antibacterial and anti-inflammatory. This study aims to determine the effect of soaking duck eggs in noni leaf extract on the interior quality of duck eggs. This study used a completely randomized design (CRD) with a concentration of 45% noni leaf extract with immersion time of 0, 12, 24, 36 and 48 hours with 3 replications. To determine whether there is a difference in treatment using Duncan Multiple Range Test (DMRT) at the level of = 0.05. The research variables are Albumen Insex, Yolk Index and Haugh Index. Data were analyzed by SPSS. The results of the study concluded that soaking duck eggs with noni leaf extract for 12-48 hours with an extract concentration of 45% had no significant effect on egg quality in this case the yolk index, albumen index and Haugh index.

PENGARUH METODE EKSTRAKSI TERHADAP KADAR TOTAL FENOLIK, KADAR TOTAL FLAVONOID DAN AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN MENGKUDU (Morinda citrifolia L.)

ABSTRACT
 
 Excessive Reactive Oxygen Species (ROS) can cause hypertension. Prevention of the risk of
 hypertension is consume an antioxidant. Noni (Morinda citrifolia L). is a plant that empirically
 proven to lower blood pressure and has antioxidant activity. Antihypertensive activity of noni
 leaves is influenced by phenolic and flavonoid compounds. The extraction method affects the levels
 of compound in extracts. The aims of study is determine the effect of the extraction method on the
 total phenolic level, total flavonoid level and antioxidant activity of noni leaf extract. The
 extraction methods are maceration, Microwave Assisted Extraction and Soxhlet. The results
 showed that the different extraction methods affected the total phenol level, total flavonoid level
 and antioxidant activity of the noni leaf extract. The Soxlet method provides the highest total
 phenolic level, total flavonoid level and antioxidant activity than other extraction methods.
 
 Keywords: Noni leaf extract, extraction method, total phenolic level, total flavonoid level and
 antioxidant activity

The effect of Noni (Morinda citrifolia) leaf shoot meal as an antioxidant and antibacterial against Salmonella sp. and Escherichia coli

Indonesia as a tropical country is rich in biological resources, among which are known as herbal plants. One of the herbs that are often used is Noni (Morinda citrifolia), which has been reported to possess antioxidant and antimicrobial activities. The objective of this study was to evaluate the antioxidant and antibacterial effects of Noni Leaf Shoot Meal (NLSM) to inhibit Salmonella sp. and Escherichia coli as poultry ration. Two hundred and forty kampong chickens of 4 weeks old were reared until 12 weeks old. The diet treatments were R0= 0% NLSM (control), R1= 0.5% NLSM, R2= 1% NLSM, R3= 1.5% NLSM, R4= 2% NLSM, and R5= 2.5% NLSM. Six treatments and four replications are used with ten kampung chickens per replicate. Data were analyzed using descriptive statistics. The parameters measured were phytochemical analysis, vitamin A, beta carotene, Malondialdehida (MDA) blood, inhibition zone diameter, and Clearance test. Results showed that NLSM could reduce MDA blood and bacterial concentration. It is concluded that lower MDA concentration in kampong chicken blood indicates that NLSM contains high antioxidants. NLSM can reduced bacterial concentration in chicken blood so it can be used as a natural antibacterial to improve kampong chickens production and immune system.

The Effect of Dosage and Method of Providing Cinnamononi Extract to Broiler’s Performance and Carcass’ Characteritic

This study was conducted to determine the effect of dosage and method of providing Cinnamononi Extract as feed additive to Broiler’s performance, and carcass’ characteristic. Cinnamononi Extract (CE) is an extract of cinnamon leaf, noni leaf, and noni fruit mixture at a ratio of 1:2:2. This experiment used a Complete Randomized Design (CRD) with 7 treatment combinations and 4 repetitions on 140 seven days aged chicks (strain AA). The treatment combinations were: T1 = without CE and without fasting, T2 = 250 mg CE/kg Body Weight and without fasting, T3 = 350 mg CE/kg BW, without fasting, T4 = 250 mg CE/kg BW after fasting for 1.5 hours in the morning, T5 = 350 mgCE/kg BW after fasting for 1.5 hours in the morning, T6 = 250 mg CE/kg BW after fasting for 1.5 hours in the day time, and T7 = 350 mg CE/kg BW after fasting for 1.5 hours in the day time. CE was given everyday by dissolving it in a limited water (± 5 ml/chicken). Results indicated that the combination of dosage and method of providing CE treatments increased (p < 0,05) broiler performance (it increased BWG, but reduced feed intake and FCR), but there was no significant effect (p > 0.05) on carcass characteritic. The conclusion of this experiment was that Cinnamononi Extract could be used as growth promotter feed additive. The effective and efficient dosage and method of providing Cinnamononi Extract to increase broiler performance is 250 mg/kg body weight after fasting for 1.5 hours either in the morning or in the day time.

Ethanolic Noni (Morinda citrifolia L.) leaf extract dechlorophyllised using sedimentation process: Antioxidant, antibacterial properties and efficacy in extending the shelf‐life of striped catfish slices

SummaryAntioxidant and antimicrobial activities of ethanolic noni leaf extract (ENLE) without and with chlorophyll removal by sedimentation method were comparatively investigated. Total chlorophyll content was reduced by 82% in the top fraction (CR‐ENLE) collected after 24 h at 4 °C as compared to that of ENLE. Antioxidant and antimicrobial activities were lower in the bottom fraction rich in chlorophyll (Chlo‐ENLE) than others (P &lt; 0.05). Based on the microbiological limit, the shelf‐life of striped catfish slices pre‐treated with 400 mg kg−1 C‐R‐ENLE was extended to 9 days as compared to the 3 days recorded for the control (without pre‐treatment). Slices treated with CR‐ENLE had lower lipid oxidation than those treated with ENLE during refrigerated storage (P &lt; 0.05). The sedimentation process was therefore a potential green method for producing ENLE having improved antioxidant and antimicrobial activities without green colour. It can be used as a natural additive for shelf‐life extension of fish slices.

Morinda citrifolia (Noni) leaf extract as corrosion inhibitor for steel-reinforced concrete in saline environment

This study aimed to determine the effectiveness of Morinda citrifolia (Noni) leaf extract as corrosion inhibitor for steel-reinforced concrete exposed to saline environment. Chloride ingress in concrete structures exposed to saline environment that leads to corrosion of steel reinforcement is prevalent in many countries including the Philippines. The use of toxic organic and inorganic compounds as corrosion inhibitors is regulated in many countries due to detrimental effects on the environment. Thus, the development of green and eco-friendly corrosion inhibitor alternatives such as those derived from biodegradable plant materials is necessary. This study hypothesized that a sample concrete amended with Noni leaf extract (0.08, 0.17, 0.25, 0.33, and 0.42%) is better than ordinary Portland concrete cement as control (0%). Compression testing, colorimetric method, and steel reinforcement corrosion testing were used to measure the compressive strength, chloride penetration resistance, and corrosion rates, respectively. Results revealed that the application of > 0.33% Noni leaf extract improved the chloride penetration resistance of the concrete by 30–33% and reduced the corrosion rate on steel reinforcement by 57–59% without affecting the compressive strength of the concrete. Noni leaf extract concentration of 0.42% exhibited combined optimum compressive strength improvement, chloride penetration resistance, and corrosion inhibition. These results show that Noni leaf extract > 0.33% can protect the steel-reinforced concrete structure against corrosion in 3% sodium chloride test media under laboratory condition. This is the first report of using Noni leaf extract admixed in concrete for the protection of steel reinforcement against corrosion.

“Formulation of Morinda Wound Healing Ointment”

The biological process of Wound healing comprises the trauma and pathologic condition of the oral mucosa.For this requires proper management so the wound heal faster and without any complication.The strong indicator of wound healing is the increase in number of fibroblast cells in the oral mucosa. The remodelling phase of wound healing shows decreasing fibroblast cell proliferation and then collagen fibres are synthesized. Noni plant have tremendous medicinal uses, by observing this fact Noni leaf (Morinda citrifolia L.), a part of the noni plant, was successfully used in present research to make ointments to heal soft tissue in wounds. The leaves of Morinda have potential chemical compounds such as glycosides, sterol, amino acids and scopletin that may be useful in the wound repair process. In addition to this Morinda citrifolia leaf contains active substances such as saponin, tannin, polyphenol flavonoid, and triterpen. The purpose of this research is to discover the activeness of the Morinda citrifolia leaves extracts in normal solvent such as ethanol to make ointments in order to arrest the growth of the bacteria.[1] By using macernation extract of morinda leaves done successfully, ointment made and done the evalution test such as spreadibilty, diffusion study, washability and very important antimicrobial test. The entire test gives corresponding good result, so the present research showing good response to healing mechanism of wounds.

Phytochemical Analysis and Wound-Healing Activity of Noni (Morinda Citrifolia) Leaf Extract

ABSTRACT This study identified the phytoconstituents, and evaluated the antioxidant, anti-inflammatory, and wound healing activities of Morinda citrifolia (Noni) leaf extract. Results showed that Noni leaves contained flavonoids, alkaloids, tannins, triterpenes, saponins, and coumarins. The total flavonoids of the Noni leaf extract (NLE) was 2.649 mg RU g−1 of dry mass. NLE was active against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus, and demonstrated antioxidant and anti-inflammatory activities with the IC50 of 133.99 and 70.21 µg mL−1, respectively. NLE also had wound healing effects in mice via reducing the wound area and the histological regeneration after 11 d of treatment. Noni leaf extract caused negligible skin irritation on rabbit skin. Abbreviations: NLE, Noni leaf extract; MIC: Minimum inhibitory concentration; DPPH, 1,1-diphenyl-2-picrylhydrazyl; RU: Rutin; IC50, Half maximal inhibitory concentration.

Production of Functional Kampong Chicken Meat with Low Cholesterol, High Antioxidant, and Unsaturated Fatty Acids Fed Noni (Morinda citrifolia) Leaf Shoot Meal

The objective of this study was to evaluate the effect of noni (Morinda citrifolia) leaf shoot meal (NLSM) supplementation in the ration on the performances of kampong chickens as well as lipid, malondialdehyde (MDA), and fatty acid contents of meats. Two hundred and forty kampong chickens of 4 weeks old were reared until 12 weeks old. The diet treatments were R0: 0% NLSM (control), R1: 0.5% NLSM, R2: 1% NLSM, R3: 1.5% NLSM, R4: 2% NLSM, and R5: 2.5% NLSM. A completely randomized design (CRD) was used with six treatments and four replications with ten kampong chickens per replicate. The data were analyzed using ANOVA and if there were significant different, the data were further analyzed using Duncan’s Multiple Range Test. The variables measured were performances of kampong chickens as well as lipid, MDA, and fatty acid contents of meat. The result showed that cholesterol and LDL contents of meat were significantly reduced by feeding NLSM. Performance and HDL contents of meat were not significantly different for all diet treatments. In addition, feeding kampong chickens with ration supplemented with NLSM could reduce MDA, saturated fatty acids and increase unsaturated fatty acids contents of meat. It is concluded that 2.5% NLSM can be used to improve meat quality of kampong chicken as functional meat.

AKTIVITAS ANTIBAKTERI EKSTRAK PERASAN DAUN MENGKUDU (Morinda citrifolia L.) TERHADAP Escherichia coli SECARA IN VITRO

Noni juice can inhibit the growth of Escherichia coli bacteria. Noni juice extraction needs concentration to extract so that the preparation is more stable in the storage process. The purpose of this study was to determine the antibacterial activity and the amount of the minimum inhibitory concentration of noni juice extract from E. coli bacteria in vitro. This type of experimental research with a completely randomized one-way design. The extract was obtained by concentrating the Noni leaf extract. Extract quality control parameters include organoleptic, yield, drying shrinkage, and qualitative tests of flavonoid and anthraquinone compounds. Antibacterial activity test using the disc diffusion method with an extract concentration of 1.56%; 3.12%; 6.25%; 12.5%; and 25% with 3 replications. Positive control of amoxicillin and negative control of distilled water. Inhibition is known from the zone formed around the paper disc. The data obtained were statistically analyzed using Kruskall Wallis followed by Mann Whitney with a 95% confidence level. The results showed that the variation in the concentration of the noni juice extract had a significant effect (p &lt;0.05). The biggest inhibitory zone was seen at 25% concentration of 10.16 mm and included in the strong category. The minimum inhibitory power was produced at a concentration of 3.12% at 2.50 mm with a weak treatment category.

Aktivitas Antibakteri Kombinasi Ekstrak Daun Mengkudu (Morinda citrifoli L.) dan Bawang Putih (Allium sativum) terhadap Escherichia coli dan Staphylococcus aureus

Indonesia is a nation with rich medicinal plants. Medicinal plants can be used for the treatment of diseases due to bacterial infection. Noni leaf ( Morinda citrifolia ) and garlic ( Allium sativum ) are medicinal plants and herbs that have the potential to inhibit or kill Escherichia coli and Staphylococcus aureus . The aim of this research is to know the antibacterial activity on the combination of extract noni leaf ( Morinda citrifolia ) and garlic ( Allium sativum ) to growth of Escherichia coli and Staphylococcus aureu s . In this study using a research design that is a complete randomized design with purposive judgment sampling method.Anti-bacterial activity test performed by the disk diffusion method. The concentration of extract used is 10%, 30%, 60% and 90%. The analysis was done by Kruskal-Wallis test. The results of this study showed that a combination of 30% leaf extract and 10% garlic extract able to inhibit the growth of Staphylococcus aureus bacteria optimally with the average area of the inhibit zone is 12 mm. While on the combination of 30% noni leaf extract and 90% garlic extract able to inhibit the growth of Eschericia coli optimally with the average area of inhibit zone is 22,27 mm.