Non-conventional Methods Research Articles

Applications of Oleuropein

One of the most prevalent phenolic compounds found in olive leaves is oleuropein. Numerous studies have demonstrated the biologically significant effects of this compound, including anti-inflammatory, anti-atherogenic, anticancer, antimicrobial, and antiviral effects, which has led to its increased attention in the scientific community. Oleuropein can be recovered and purified (mostly by chromatographic techniques) from a variety of sources using both conventional and non-conventional methods. It can then be applied in a number of contexts. Because of its numerous pharmacological properties, oleuropein is commercially obtainable as a food enhancement in Mediterranean countries. Numerous scientific and clinical investigations have demonstrated their efficacious significance.

Green and sustainable technologies for extraction of carotenoids from natural sources: a comprehensive review.

In recent years, driven by increasing consumer demand for natural and healthy convenient foods, the food industry has been shifting from synthetic to natural products. This shift is also reflected in the growing popularity of non-conventional extraction methods for pigments, which are favored for sustainability and environment-friendliness compared to conventional processes. This review aims to investigate the extraction of carotenoids from a variety of natural sources, including marine sources like fungus, microalgae, and crustaceans, as well as widely studied plants like tomatoes and carrots. Additionally, it delves into the recovery of valuable carotenoids from waste products like pomace and peels, highlighting the nutritional and environmental benefits. The review also emphasizes the role of green solvents such limonene, vegetable oils, ionic liquids, supercritical fluids, and natural deep eutectic solvents in effective and ecologically friendly carotenoid extraction. These technologies support the ideas of a circular and sustainable economy in addition to having a smaller negative impact on the environment. Overall, the present study highlights the crucial importance of green extraction technologies in achieving the dual goals of sustainability and public safety.

Progress on nonculture based diagnostic tests for invasive mould infection.

This review describes the current status of diagnosing invasive mould disease and Pneumocystis pneumonia using nonconventional diagnostics methods. There has been significant development in the range of nonculture mycological tests. Lateral flow tests (LFTs) for diagnosing aspergillosis complement galactomannan ELISA testing, and LFTs for other fungal diseases are in development. Rapid and low through-put B-D-Glucan assays increase access to testing and there has been significant progress in the standardization/development of molecular tests. Despite this, no single perfect test exists and combining tests (e.g., antigen and molecular testing) is likely required for the optimal diagnosis of most fungal diseases. Based on established clinical performance few mycological tests can be used alone for optimal diagnosis of fungal disease (FD) and combining tests, including classical approaches is the preferred route for confirming and excluding disease. Next-generation sequencing will likely play an increasing role in how we diagnose disease, but optimization, standardization and validation of the entire molecular process is needed and we must consider how host biomarkers can stratify risk. Given the burden of FD in low- and medium-income countries, improved access to novel but more so existing diagnostic testing is critical along with simplification of testing processes.

Anti-inflammatory and anti-hyperglycemia effects of mealworm (Tenebrio molitor larvae) protein extracted by four methods: alkali, salt, enzyme, and screw press.

The use of edible insect protein in food products is contingent on their biological effects. Conventional protein extraction methods are not only time-consuming and costly but also energy-intensive. There is a need for alternative techniques that maintain the bioactivities of insect proteins and are environmentally sustainable. This study compares the health functionality of mealworm (Tenebrio molitor larvae) concentrates obtained by conventional methods-alkali and salt (MS) extraction-and nonconventional methods-enzyme (ME) and screw press (MP)-to enhance their applicability despite lower protein concentration. Overall, MP exhibited the highest essential amino acids content, whereas ME showed the highest in vitro digestibility, total phenolic contents, and antioxidant capacities among all the concentrates. ME also had a significant cell proliferative capacity at concentrations ≥500µg/mL. MS significantly inhibited tumor necrosis factor-alpha and interleukin-1beta secretion in lipopolysaccharide-treated Hep3B cells compared to other samples. As for anti-hyperglycemia effects, treatment with MS and ME for 2 and 5min significantly increased the p-Akt/Akt ratio (MS, 1.34- and 1.61-fold; ME, 2.26- and 2.70-fold, respectively). In conclusion, enzyme treatment enhanced nutritional value and antioxidant capacity, whereas salt treatment potentially contributed to anti-inflammatory and anti-hyperglycemia activities. Hybrid extraction techniques combining conventional and nonconventional methods are suggested based on target applications, considering health benefits, environmental impact, costs, and efficiencies. PRACTICAL APPLICATION: Four mealworm protein extraction methods (alkali/salt/enzyme/screw press) were compared for their nutritional and biological properties. Alkali extraction enhanced protein content, enzyme treatment improved nutritional value and antioxidant capacity, and salt-assisted extraction exhibited immunomodulatory effects in vitro. Notably, enzyme and salt treatments produced protein concentrates with significant antidiabetic and anti-hyperglycemic properties.

Hydrogen Generation by Photolysis of Water Vis-à-Vis Other Conventional and Advanced Non-conventional Methods of Hydrogen Production—A Review

Hydrogen Generation by Photolysis of Water Vis-à-Vis Other Conventional and Advanced Non-conventional Methods of Hydrogen Production—A Review

Optimization of Phenolic Compounds Extraction from Aerial Parts of Fabiana punensis S. C. Arroyo by Ultrasound- and Microwave-Assisted Extraction.

Fabiana punensis S. C. Arroyo is a subshrub or shrub that is indigenous to the arid and semiarid region of northern Argentina and is known to possess several medicinal properties. The objective of this study was to optimize the extraction conditions so as to maximize the yield of bioactive total phenolic compound (TPC) and flavonoids (F) of F. punensis' aerial parts by using non-conventional extraction methods, namely ultrasound-assisted extraction, UAE, and microwave-assisted extraction, MAE, and to compare the biological activities and toxicity of optimized extracts vs. conventional extracts, i.e., those gained by maceration. Response Surface Methodology (RSM) was used to apply factorial designs to optimize the parameters of extraction: solid-to-liquid ratio, extraction time, ultrasound amplitude, and microwave power. The experimental values for TPC and F and antioxidant activity under the optimal extraction conditions were not significantly different from the predicted values, demonstrating the accuracy of the mathematical models. Similar HPLC-DAD patterns were found between conventional and UAE- and MAE-optimized extracts. The main constituents of the extracts correspond to phenolic compounds (flavonoids and phenolic acids) and apigenin was identified. All extracts showed high scavenger capacity on ABTS•+, O2•- and H2O2, enabling the inhibition of the pro-inflammatory enzymes xanthine oxidase (XO) and lipoxygenase (LOX). They also showed an antimutagenic effect in Salmonella Typhimurium assay and cytotoxic/anti-proliferative activity on human melanoma cells (SKMEL-28). Toxicological evaluation indicates its safety. The results of this work are important in the development of efficient and sustainable methods for obtaining bioactive compounds from F. punensis for the prevention of chronic degenerative diseases associated with oxidative stress, inflammation, and DNA damage.

Phytochemical, Antimicrobial, and Antioxidant Activity of Different Extracts from Frozen, Freeze-Dried, and Oven-Dried Jostaberries Grown in Moldova.

In this paper, the qualitative and quantitative profile is evaluated of the bioactive compounds, antioxidant activity (AA), microbiostatic properties, as well as the color parameters of jostaberry extracts, obtained from frozen (FJ), freeze-dried (FDJ), and oven-dried berries (DJ). The optimal extraction conditions by ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) were selected after determination of the total polyphenol content (TPC), total flavonoid content (TFC), total antocyanin content (TA), AA by 2,2-diphenyl-1-picrylhydrazyl-hydrate (DPPH), and the free radical cation 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonates (ABTS). Non-conventional extraction methods are less destructive to anthocyanins, while drying the berries reduced TA, regardless of the extraction method. The oven-drying process reduced the concentration of TA in DJ extracts by 99.4% and of ascorbic acid by 92.42% compared to FJ. AA was influenced by the jostaberry pretreatment methods. The DPPH and ABTS tests recorded values (mg Trolox equivalent/g dry weight) between 17.60 and 35.26 and 35.64 and 109.17 for FJ extracts, between 7.50 and 7.96 and 45.73 and 82.22 for FDJ, as well as between 6.31 and 7.40 and 34.04 and 52.20 for DJ, respectively. The jostaberry pretreatment produced significant changes in all color parameters. Mutual information analysis, applied to determine the influence of ultrasound and microwave durations on TPC, TFC, TA, AA, pH, and color parameters in jostaberry extracts, showed the greatest influence on TA (0.367 bits) and TFC (0.329 bits). The DPPH and ABTS inhibition capacity of all FJ' extracts had higher values and varied more strongly, depending on pH, heat treatment, and storage time, compared to the AA values of FDJ' and DJ' extracts. A significant antimicrobial effect was observed on all bacterial strains studied for FJP. FDJP was more active on Bacillus cereus, Staphylococcus aureus, and Escherichia coli. DJP was more active on Salmonella Abony and Pseudomonas aeruginosa. The antifungal effect of DJP was stronger compared to FDJP. Jostaberry extracts obtained under different conditions can be used in food production, offering a wide spectrum of red hues.

Political Economy of Manufacturing: Conventional and Nonconventional Practices in the Global Context

This paper examines the intricate interplay between political economy and manufacturing practices, encompassing both conventional and nonconventional methods in a global context. It explores how political and economic factors shape manufacturing strategies, with a focus on technological innovation, labor dynamics, sustainability, and regulatory frameworks. Comparative case studies highlight disparities between developed and developing economies, revealing distinct approaches to industrial policies and their impacts on economic outcomes. The findings underscore the importance of balancing traditional manufacturing strengths with advancements in Industry 4.0 technologies to enhance competitiveness and sustainability. Policy recommendations advocate for strategic government interventions that promote innovation, support workforce adaptation, and foster international collaboration in manufacturing sectors.

Design and Material Optimization of Oil Plant Piping Structure for Mitigating Erosion Wear

Erosion in piping structures poses a significant challenge for oil industries as the conveyance of solid particles leads to operational malfunctions and structural failures affecting the overall oil plant operation. Conventional oil recovery methods have historically dominated, while in response to the challenges imposed by declining conventional oil production, the global shift towards non-conventional methods necessitates a reevaluation of erosion mitigation strategies due to increased piping infrastructure. Therefore, in this study research has been conducted to reduce erosion and optimize the piping structure. Variables impacting the erosion in piping were investigated from the literature, and simulation cases were made based on the impacted variables. Computational Fluid Dynamics (CFDs) analysis was performed using the Discrete Phase Model (DPM) to determine the erosion wear rate in each simulation case; based on the CFD results, variables with low Turbulent Dissipation Rates (TDRs) and Erosion Rates (ERs) were determined, and the optimized piping structure was designed. As a result, the optimized piping structure showed an 80% reduction in the turbulent dissipation rate and a 99.2% decrease in the erosion wear rate. These findings highlight a substantial improvement in erosion control, ensuring the safety and longevity of piping structures in oil plant operations.

Ultrasound Microwave Assisted Extraction Pada Daun Serai Wangi Menggunakan Pelarut Ionik.

Leaves of citronella (Cymbopogon nardus L.) have pretty much essential oil. Essential oil from citronella leaves is generally extracted by conventional methods. Conventional method is still less effective. The problem can be overcome by using non-conventional methods in the form of UMAE (Ultrasound-Microwave Assisted Extraction). UMAE is an extraction process assisted by ultrasound and microwaves. NaCl solution can be used as a solvent in the UMAE method because NaCl has a large dielectric constant. The aim of this study was to examine the effect of extraction time and the addition of salt on the yield and color of essential oils produced using the UMAE. The extraction process begins with the raw material preparation stage, such as harvesting, size reduction, and drying. Then, pre-treatment was carried out using ultrasound for 5 minutes at 35oC with the addition of 2, 4, 6, 8, and 10% (w/v) salt. Pre-treatment results were followed by the extraction process using microwaves for 30, 45, 60, 75, and 90 minutes. The best results were obtained with the addition of 6% salt and 60 minutes of extraction time with a feed to solvent ratio of 0.1 with a yield of 1.975% and the color is yellow.

Experimental Study on Electro-Discharge Drilling of NiTiCu10 Shape Memory Alloy

Shape memory alloys (SMAs) are potential materials in various areas such as engineering and medicine, and their applications are being studied for practical use. SMAs show competence in their main material properties in response to their working environment or external stimuli. Machining of NiTiCu10 SMAs is difficult using traditional machining because of their wide-ranging mechanical properties like high toughness, strength, and sensitivity to phase transformation temperature. Nonconventional machining methods such as electro-discharge machining (EDM) are suitable for effective machining SMAs. The work material NiTiCu10 SMA has been used in this study, and processed using the vacuum induction melting (VIM) technique. The addition of copper leads to increase in the martensitic transformation temperatures. This paper focuses on the electro-discharge drilling (EDD) of NiTiCu10 SMA using copper tool electrode. Experimental analysis of performance criteria has been evaluated by conducting experiments following one factor at a time (OFAT) approach. Machining features such as material removal rate (MRR), tool electrode wear rate (TEWR), and surface roughness (SR) have been studied by considering pulse current ([Formula: see text]), gap voltage ([Formula: see text]), pulse on time ([Formula: see text]), pulse off time ([Formula: see text]), and rotational speed of tool electrode (N). Experimental results have shown that machining at the highest [Formula: see text] of 12[Formula: see text]A yields the highest MRR with the value of 4.077[Formula: see text]mm3/min, whereas mcahining at [Formula: see text] of 15[Formula: see text][Formula: see text]s yields the lowest TEWR with the value of 0.031[Formula: see text]mm3/min. The lowest SR is 2.8[Formula: see text][Formula: see text]m achieved at the lowest [Formula: see text] of 15[Formula: see text][Formula: see text]s. Surface morphology is significant in quality evaluation in the manufacturing, and building industries because it directly determines the mechanical performance of parts, and the service life of products. Morphological investigation via scanning electron microscope (SEM) has confirmed the formation of craters, debris, microcracks, and resolidified layers. The benefit of this study has been that we have been able to select the range of significant control factors, and predict their levels, for decisive experimentation.

Editorial: Improving protein functionality using conventional and non-conventional methods

Editorial: Improving protein functionality using conventional and non-conventional methods

Psychotherapist factors that patients perceive are associated with treatment failure.

Psychotherapy is a well-established and effective treatment for various psychiatric problems, but a substantial proportion of patients do not benefit from it, and many terminate treatment prematurely. Previous studies suggest that therapist dissatisfaction may play a pivotal role in premature treatment termination. This study, therefore, aimed to investigate therapist factors that may contribute to less-than-optimal results and dropout. Data were collected through a survey of patients (n = 736) with the experience of previous unsuccessful psychotherapy treatments. Based on prior research, the survey covered 13 therapist behaviors and traits, such as being unstructured or unengaged. The most common therapist factor that patients associated with treatment failure was poor assessment/understanding (86.7%), followed by inflexibility (71.7%) and poor knowledge (70.1%). Furthermore, this study identified four novel therapist-related factors: breaking the treatment contract, inappropriate sexual behaviors or comments, using non-conventional methods, and dominating behaviors. Overall, this study highlights the significance of therapist-related factors in premature treatment termination and treatment failure, shedding light on the crucial role therapists play in the therapeutic process. Understanding these factors is essential for improving psychotherapy outcomes and reducing dropout rates. Further investigations are needed to explore the impact of these therapist behaviors on treatment outcomes and to develop strategies for enhancing therapist competencies and skills to foster a more effective therapeutic alliance. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Genomic Advances in Biofortification of Iron and Zinc in Wheat (Triticum aestivum L.)

Majority of population that depend on wheat are found to be deficient in iron and zinc. About 20% of calories worldwide are derived from wheat. Milling of wheat reduces the bioavaibility of Fe and Zn in wheat grains. Micronutrient deficiency is the main cause of malnutrition worldwide and hidden hunger in underdeveloped countries. To overcome this problem it becomes necessary to develop varieties that are rich in iron and zinc content. Biofortification gets around these issues by enhancing the micronutrient content of wheat. The genetic approaches of biofortification particularly the non-conventional methods have proved to be the most efficient ones. These method includes QTL mapping approach, GWAS and transgenics. Although conventional methods for a long time has been used for developing varieties of wheat rich in Fe and Zn but conventional breeding for certain traits may be inconvenient and time-consuming and are prone to errors due to their reliance on traditional phenotyping or visual assessments. Several research have been undertaken to map QTLs of Fe and Zn concentration in wheat grains and have been used in elite genetic backgrounds to improve micronutrient content. Genomic studies revealed through GWAS exhibit high QTL resolution and allele coverage, which can be used for improvement of Fe and Zn content in wheat. Genetic engineering techniques or transgenics approaches allows genetic alteration of crops to improve the nutritional quality of wheat. Genomic approach of biofortification of wheat has proved to be the most efficient methods for increasing iron and zinc content in the crop.

Optimization of Extraction Conditions of Bioactive Compounds From Kurdistan Species Urtica dioica.

Phytotherapeutics derived from medicinal plants treat various illnesses, including viral infections such as SARS, MERS, and SARSCoV-2, as well as bacterial and fungal diseases. It highlights ongoing research into the chemical compositions of plant components for developing new drugs, with a particular emphasis on anti-cytotoxic agents for anticancer drugs. Traditional extraction methods have limitations, leading to the exploration of environmentally friendly technologies such as ultrasound-assisted, supercritical fluid, microwave-assisted, and accelerated solvent extraction. The paragraph concludes by stating the aim of a specific study to optimize extraction conditions of bioactive compounds from Urtica dioica in Kurdistan, comparing conventional and non-conventional extraction methods, solvents, and extraction times. The study was conducted between June 2022 and August 2022, fresh leaves and stems of U. dioica plant were collected and sequentially underwent four extraction methods (maceration, Soxhlet, ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE) by using petroleum ether, chloroform, ethanol, and distilled-water as solvents. The results highlighted significant variations in the yields of bioactive compounds based on the extraction method, solvent type, and duration. Among conventional methods, Soxhlet was the most powerful method and had the most extraction yields, while maceration had the lowest yields. The modern techniques surpassed the conventional methods by producing high extraction yields within a shorter time (a few minutes) and using a lesser amount of solvent. Consequently, UAE and MAE emerge as the most efficient techniques. Hence, MAE effectively produced the highest extraction yields and is considered the preferred technique. The choice of solvents significantly influenced the extraction yields, with ethanol consistently emerging as an effective solvent across various extraction methods. In contrast, petroleum ether demonstrated the lowest efficacy as a solvent. Furthermore, the results unveiled the impact of extraction time on yields, indicating a correlation between increased time and extraction yield in certain cases. Extraction is a very critical step in the study of medicinal plants. The amount of extracted compounds is significantly affected by the extraction method, solvent, and time. Ethanol stands out as the most effective solvent, producing the highest yields of bioactive compounds, while petroleum ether yields the least. Additionally, extraction yield shows a direct relation with extraction time. Soxhlet being the most powerful among conventional methods and maceration yields the least. Modern techniques, particularly UAE and MAE, surpass conventional methods by achieving high yields in shorter times with less solvent. MAE, in particular, offers advantages such as shortened extraction time, increased efficiency, reduced labor, and enhanced selectivity, making it the preferred method for extracting bioactive compounds from aerial parts U. dioica.

The Role of Thermal and Electrical Energies to Increase the Pepper Quality: A Review

Indonesia is one of the most prominent pepper producers and consumers worldwide. Indonesian pepper quality, however, is still low. The pepper’s low-quality problem is caused by the processing that still uses the traditional (conventional) method. This study reviews many techniques that have the potential to improve pepper quality. The review result from the references shows that waiting time (delaying) in the ripening process can increase the pepperine level from 4.33 % to 6.60 %. The pepperine level is the most vital parameter to show pepper quality. However, a more comprehensive result that compares the conventional and non-conventional methods for all kinds of pepper quality parameters still could not be obtained.

Synthesis of propargylamine: pioneering a green path with non-conventional KA2 coupling approach.

The KA2 coupling reaction is a well-explored and versatile method for forming C-C bonds in synthetic chemistry. It is composed of ketone, amine, and alkyne, which play a major role in the synthesis of propargylamines, known for their diverse biological activities and are used in treating neurogenetical disorders. The KA2 coupling is highly challenging due to the low reactivity of ketimines toward nucleophilic attacks with metal acetylide intermediates formed by activating the C-H bond of the alkyne. Despite predominant studies conducted on thermal conditions for KA2 coupling reactions, green and sustainable approaches like non-conventional methods still have a lot to achieve. This review article provides a comprehensive introduction to the non-conventional approach in the KA2 coupling reaction, outlining its mechanisms and exploring future aspects.

Advancements in genetic techniques and functional genomics for enhancing crop traits and agricultural sustainability.

Genetic variability is essential for the development of new crop varieties with economically beneficial traits. The traits can be inherited from wild relatives or induced through mutagenesis. Novel genetic elements can then be identified and new gene functions can be predicted. In this study, forward and reverse genetics approaches were described, in addition to their applications in modern crop improvement programs and functional genomics. By using heritable phenotypes and linked genetic markers, forward genetics searches for genes by using traditional genetic mapping and allele frequency estimation. Despite recent advances in sequencing technology, omics and computation, genetic redundancy remains a major challenge in forward genetics. By analyzing close-related genes, we will be able to dissect their functional redundancy and predict possible traits and gene activity patterns. In addition to these predictions, sophisticated reverse gene editing tools can be used to verify them, including TILLING, targeted insertional mutagenesis, gene silencing, gene targeting and genome editing. By using gene knock-down, knock-up and knock-out strategies, these tools are able to detect genetic changes in cells. In addition, epigenome analysis and editing enable the development of novel traits in existing crop cultivars without affecting their genetic makeup by increasing epiallelic variants. Our understanding of gene functions and molecular dynamics of various biological phenomena has been revised by all of these findings. The study also identifies novel genetic targets in crop species to improve yields and stress tolerances through conventional and non-conventional methods. In this article, genetic techniques and functional genomics are specifically discussed and assessed for their potential in crop improvement.

Material testing for injection molded and 3D printed pristine/glass reinforced nylon at various strain rates

With the advent of new polymer material and non-conventional methods of manufacturing, the effect of loading conditions on mechanical properties has now been in huge demand to facilitate their industrial applications. Present paper has been focused on investigating the strain rate effects on mechanical properties of pristine nylon-6 and 20% glass reinforced nylon-6 material fabricated using injection molding and SLS (Selective Laser Sintering) printing manufacturing methods, respectively, at three strain rates of 0.0003, 0.003, and 0.01 per second. Tensile strength of injection molded nylon-6 has shown a positive relationship with strain rate whereas compressive strength has shown a reciprocal effect. At highest strain rate of 0.01 s−1, tensile strength exhibits 10.27% more as compared to lowest strain rate of 0.0003 s−1. Tensile strength at highest strain rate is found 60 MPa whereas at lowest strain rate it exhibits 54.41 MPa. At higher values of strain rate, rising of maximum strain is found very much sensitive than the rise in tensile strength. It also shows high percentage of deformation at higher value of strain rate. On the other hand, SLS printed 20% glass reinforced nylon-6 shows variable tensile strength with respect to strain rate due to uneven distribution of glass particles into nylon matrix created due to manufacturing process of SLS printing. FESEM images shows the cavity formation as observed before loading conditions whereas the glass fracturing phenomenon observed at the after loading conditions. However, compressive strength is found minimal with strain rate with an average value of 175.61 MPa. The findings of this study have contributed in avoiding the improper selection of material and its strain rate application during numerical modeling for industrial application purpose.

Geography-based transmission network expansion strategies for Andean electrical systems

In many countries of the Global South, transmission network expansion faces unique challenges due to the diversity of available resources and specific geographic conditions. These challenges are particularly evident in regions with complex geographies, such as the Andean region, where elevation differences and irregular topography pose significant obstacles to the development of electricity infrastructure. This study addresses these challenges by applying non-conventional strategies for transmission grid expansion, with a particular emphasis on adapting to the unique geographic features of the Andean region. To illustrate this, a case study called EC-45 bus system is developed using Ecuador as a country representative of the geographic diversity of the Andean region. Furthermore, this work presents a Mixed Integer Linear Programming (MILP) model that prioritizes the repowering and reconfiguration of existing lines over the construction of new lines. The methodology employed evaluates the investment required for the construction of new lines, considering construction costs for three site types (<1000 meters above sea level (m.a.s.l.), ≥1000 m.a.s.l., and combined). Quantitative results show that using non-conventional solution methods can generate savings of up to 30.60% in investment costs compared to the solution provided by the Linear Disjunctive model. This approach offers a way towards more efficient management of resources, especially in countries with complex geographies such as the Andean one.