Introduction T h e observation (Weijer , 1961; Weijer et al., 1963, 1961, 1965), that different modes of lcaryokinesis are involved in the differentiation of specific n~orphological struct;res of the vegetative cycle of Nez~rosporrr crassa; raises the immediate question whether anv of these non-classical modes of nuclear division extend h t o the reproductive cycle of the mould. Although Singleton (1953) in his description of the chromosome cycle in the ascus of N . cmssn states that as the spore matures after division IV , its cytoplasm, originally rather uni forn~ly granular o r finely in appearance, beconles highly vacuolate (p. 137), it was felt that this information on the last interphase of a scos~oro~enes i s n7as too incomplete to be useful for a critical assessment of the prob/em Gated above. ~ h e r e f A r e , a detailed study was made of the late telophase-interphase nuclei of division I V in the devel6ping ascus. Weijer (1964) and Weijer et al., (1964, 1965) observed in the juvenile cycle of N . cmssn ring shaped nuclear configurations, which are usually diffuse in character early in Icaryolcinesis, whereas in more advanced stages, the nuclear material is separated into eight Feulgen stainable bodies (seven chromosomes and a centriole) surrounding a non-stainable nucleolus. Similar ring shaped nuclei were described b y Balcerspigel (1958, 1959) in Gelclsi~~ospovn tetrrrsperma Dowd., and in Endogolze spl~ag7zophila Atlc., and b y Robinow (1957, 1962) in il4ucor hie7nnlis and i M . fragilis. Hall (1963), in his studies concerning the cytology of iMo7zili7tin fructicolrr (Wint . ) Honey., did not observe ring shaped nuclei, although the published photograph of interphase nuclei is suggestive f o r their occurrence. Since the ring shaped nucleus is an easily recognizable structure of the juvenile cvclc, a search for the occurrence of this nuclear configuration was initiated in the developing ascus. Material and lMethods Crosses \vere made between strain F G S C 262 and F G S C 16 on liquid crossing medium (Stanford Neurospora A~lcthods, 1964). Half-pint milk bottles containing 70 ml. of crossing medium \irere inoculated with hvphal material of strain F G S C 16 on a partly submerged filter paper square (7 s 8.~111.) and incubated at 23°C for 6-7 days to allow the develop~nent of protoperithecia. Conidia of strain F G S C 262 werk then dusted on to the resulting growth. T h e cultures \i.erc then reincubated a t 23°C fo r 128 to 130 hours, after which the perithecia \.ere removed and the asci wcre dissected on to a covers l i~ . After 1 drying at 60°C, the preparations \irere stained according to the Feulgen technique as outlincd bv h/IcDonald and Weijer (1965, in preparation). Observations and microphoto~raphs \irere made with a Leitz Ortholux I1 microscope fitted wit11 a Lcitz plano objective (100 x N.A. 1.32) and a Leitz Orthomat 35 mm. automatic camera. Balzers interference filters (540 and 576 mu) wcre used.
Read full abstract