The total alkaloids obtained from opium by solvent extraction was packed in a column, connected to the top of an acid column (pH 2.2) which in turn was connected to the top of an alkali column (pH 12.5), all standing vertically (Fig. 1). These were developed with ether to separate the alkaloids into amphoteric and non-amphoteric bases, the acid column was separated, and this column was eluted by the procedure described in Part I of this series to collect fractions of crude morphine, narcotine, papaverine, crude thebaine, and crude codeine. Crude morphine, thebaine, and codeine were purified by rechromatography through columns of respective pH of 3.5, 5.0, and 3.8. The pure alkaloids thereby obtained were determined by non-aqueous titration. The manner of separation of these alkaloids is presented in Chart 1.