Non-toxic Effect Research Articles

5-Fluoro/(trifluoromethoxy)-2-indolinone derivatives with anti-interleukin-1 activity.

The pro-inflammatory cytokine interleukin-1 (IL-1) drives the pathogenesis of several inflammatory diseases. Recent studies have revealed that 2-indolinones can modulate cytokine responses. Therefore, we screened several 2-indolinone derivatives in preliminary studies to develop agents with anti-IL-1 activity. First, the putative efficacies and binding interactions of 2-indolinones were evaluated by docking studies. Second, previously synthesized 5-fluoro/(trifluoromethoxy)-1H-indole-2,3-dione 3-(4-phenylthiosemicarbazones) (compounds 47-69) which had the highest inhibitory effect in the screening were evaluated for inhibitory effects on the IL-1 receptor (IL-1R). Compounds 52 (IC50 = 0.09 µM) and 65 (IC50 = 0.07 µM) were selected as lead compounds for the subsequent synthesis of new derivatives. The novel 5-fluoro/(trifluoromethoxy)-1H-indole-2,3-dione 3-(4-phenylthiosemicarbazones) (compounds 70-116) were designed, synthesized, and in vitro studies were completed. The compounds 76, 78, 81, 91, 100, 105, and 107 tested showed nontoxic inhibitory effects on IL-1R-dependent responses in the range of 0.01-0.06 µM and stronger than the lead compounds 52 and 65. In vitro and in silico findings showed that compounds 78 (IC50 = 0.01 µM) and 81 (IC50 = 0.02 µM) had the strongest IL-1R inhibitory effects and the most favorable drug-like properties. Molecular modeling studies of the compounds 78 and 81 were carried out to determine the possible binding interactions at the active site of the IL-1R.

In vitro establishment of cell suspension culture of Ceropegia bulbosa for improved production of cerpegin content through elicitation of engineered carbon and ZnO nanoparticles.

The present investigations aimed to conserve C. bulbosa a threatened plant species and for production of cerpegin through cell culture technology using ENP elicitation. Leaf explants were aseptically cultured with normal MS medium-supplemented PGRs BA and NAA various concentrations, and the best callus induction response was recorded on 4.5 + 4.5 μM. The prospective special effects of the ENPs on plant cell cultures are the key part of our study and used to evaluate leaf callus culture proliferation with the reduction of browning, establishment, biomass, and metabolite formation. The CNP concentrations (0, 2, 4, 8, 12 mg/l) are used for the callus proliferation and browning reduction. The cell suspension cultures are also established, and they were elicited with EZnONPs (0, 25, 50, 100, 150 mg/l) for evaluation of biomass, antioxidant, non-antioxidant enzyme activation, toxicity, ROS defense activation, and metabolite development in cell cultures. The metabolite extraction, UV, and NMR characterization confirm that the toxic and nontoxic effect of ENPs on leaf cell cultures varies with high to low concentration.

Spectrophotometrically, Spectroscopically, Microscopically and Thermogravimetrically Optimized TiO2 and ZnO Nanoparticles and their Bactericidal, Antioxidant and Cytotoxic Potential: A Novel Comparative Approach.

Current study was aimed to determine the antibacterial, antioxidant and cytotoxic potential of Titanium dioxide nanoparticles (TiO2NPs) and Zinc oxide nanoparticles (ZnONPs). Nanoparticles were characterized by UV-Vis spectrophotometry, particle size analyzer (PSA), fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy(SEM), and thermogravimetric analysis (TGA). The Minimum inhibitory concentration (MIC) was determined by standard agar dilution method. Antibacterial potential of nanoparticles was analyzed by standard disc diffusion method against bacterial strains including Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumonia. Different concentrations of NPs (0.2, 0.4, 0.6, 0.8, 1.0, 1.2 and 1.4mg/mL) were incorporated to evaluate the antimicrobial activity. Antioxidant activity and cytotoxicity of these NPs was analyzed by DPPH method and brine shrimp cytotoxicity assay, respectively. The MIC of TiO2NPs against E. coli, P. aeruginosa and K. pneumoniae was 0.04, 0.08 and 0.07mg/mL respectively while the MIC of ZnONPs against the above strains was 0.01, 0.015 and 0.01mg/mL. The maximum zone of inhibition was observed for K. pneumoniae i.e., 20mm and 25mm against TiO2 and ZnO NPs respectively, at 1.4mg/mL concentration of NPs. The susceptibility of NPs against bacterial strains was evaluated in the following order: K. pneumoniae > P. aeruginosa > E. coli. The antioxidant activity of nanoparticles increased by increasing the concentration of NPs while cytotoxic analysis exhibited non-toxic effect of ZnO NPs while TiO2 had toxic effects on 1.2 and 1.4mg/mL concentrations. Results revealed that ZnO NPs have moreantibacterialand negligiblecytotoxic potential in contrast to TiO2 NPs.

APPA Increases Lifespan and Stress Resistance via Lipid Metabolism and Insulin/IGF-1 Signal Pathway in Caenorhabditis elegans.

Animal studies have proven that 1-acetyl-5-phenyl-1H-pyrrol-3-yl acetate (APPA) is a powerful antioxidant as a novel aldose reductase inhibitor independently synthesized by our laboratory; however, there is no current information on APPA's anti-aging mechanism. Therefore, this study examined the impact and mechanism of APPA's anti-aging and anti-oxidation capacity using the Caenorhabditis elegans model. The results demonstrated that APPA increases C. elegans' longevity without affecting the typical metabolism of Escherichia coli OP50 (OP50). APPA also had a non-toxic effect on C. elegans, increased locomotor ability, decreased the levels of reactive oxygen species, lipofuscin, and fat, and increased anti-stress capacity. QRT-PCR analysis further revealed that APPA upregulated the expression of antioxidant genes, including sod-3, gst-4, and hsp-16.2, and the critical downstream transcription factors, daf-16, skn-1, and hsf-1 of the insulin/insulin-like growth factor (IGF) receptor, daf-2. In addition, fat-6 and nhr-80 were upregulated. However, the APPA's life-prolonging effects were absent on the daf-2, daf-16, skn-1, and hsf-1 mutants implying that the APPA's life-prolonging mechanism depends on the insulin/IGF-1 signaling system. The transcriptome sequencing also revealed that the mitochondrial route was also strongly associated with the APPA life extension, consistent with mev-1 and isp-1 mutant life assays. These findings aid in the investigation of APPA's longevity extension mechanism.

In Vitro Study on Spermicidal Action of Hydro-methanol Extract of Tinospora cordifolia (Willd.) Stem in Rat and Human Sperm: a Comparative Analysis.

Human fertility regulation is a major way to control overpopulation. In this perspective, this study emphasized the in vitro effect of hydro-methanol extract of Tinospora cordifolia (TCHME) stem for spermicidal and reproductive hypo-functions using human and rat samples. Control, 0.5-, 1-, and 2-mg TCHME-charged groups were considered to assess the relevant parameters. Levels of spermiological parameters like sperm motility, viability, the integrity of plasma and acrosomal membrane, and nuclear chromatin decondensation were significantly reduced (p < 0.05) in the dose- and duration-dependent TCHME-charged groups compared to the control. The inhibitory concentration 50 (IC50) of TCHME on motile human and rat sperms were 0.8 and 0.4 mg/ml, respectively. Testicular androgenic key enzymes and antioxidant enzymes (human sperm pellet, testes, and epididymis of rat)' activities were significantly diminished (p < 0.05), while antioxidant enzymes' activities were significantly elevated (p < 0.05) in renal and insignificantly (p > 0.05) elevated in hepatic tissues of rat in TCHME-charged groups compared to the control. Significant elevation (p < 0.05) of thiobarbituric acid reactive substances (TBARS)' level in human sperm pellet, testes, and epididymis of rats and significant diminution (p < 0.05) in TBARS levels of liver and kidney were observed in TCHME-charged groups. It focused that TCHME is more potent for stress imposition on reproductive tissues and sperm compared to the other tested tissues. Non-significant alterations (p > 0.05) in glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) activities in the said organs of rat indicated its non-toxic effect. It highlighted that TCHME possesses spermicidal and reproductive tissue-specific effects which strengthen the possibilities of male contraceptive development from it.

In vitro evaluation of polycaprolactone‐based structure for guiding segmental bone defect

AbstractSegmental bone tissue engineering is a highly effective approach for the repair of large bone defects. In this article, 3D printing was used to fabricate the polycaprolactone (PCL)/NaCl‐based cylindrical structures. The effects of the structure's thickness (L) and NaCl concentration on the mechanical properties, degradability, swelling behavior, porosity, and cytotoxicity of the samples were investigated. Response surface methodology was employed to study the mechanical behavior using the central composite design (CCD). Results showed that increasing the NaCl concentration up to 10% wt significantly improved the degradability, swelling, and hydrophilicity of the structures. It was also indicated that the maximum stiffness of the guide structures under vertical loading was almost five times larger than the horizontal loading direction, but the samples showed greater compressive strength and elongation under horizontal compressive loading. Results indicated that the mechanical properties of the structures were more dependent on the structure thickness so the thicker structures with an 800 μm thickness had better mechanical properties in both vertical and horizontal loading. Cytotoxicity assay also approved the nontoxic effect of the PCL structures on the MC3T3 osteoblast cell line. Based on the results, the PCL‐based structures were a suitable candidate to act as a guide for segmental bone tissue engineering.Highlights A 3D‐printed conduit was manufactured to guide bone reconstruction Response surface methodology (RSM) using central composite design (CCD) was employed NaCl concentration and the diameter of strands were the main parameters This 3D‐printed guiding approach is useful for segmental bone tissue engineering

Conducting polymer composite-based biosensing materials for the diagnosis of lung cancer: A review

The development of a simple and fast cancer detection method is crucial since early diagnosis is a key factor in increasing survival rates for lung cancer patients. Among several diagnosis methods, the electrochemical sensor is the most promising one due to its outstanding performance, portability, real-time analysis, robustness, amenability, and cost-effectiveness. Conducting polymer (CP) composites have been frequently used to fabricate a robust sensor device, owing to their excellent physical and electrochemical properties as well as biocompatibility with nontoxic effects on the biological system. This review brings up a brief overview of the importance of electrochemical biosensors for the early detection of lung cancer, with a detailed discussion on the design and development of CP composite materials for biosensor applications. The review covers the electrochemical sensing of numerous lung cancer markers employing composite electrodes based on the conducting polyterthiophene, poly(3,4-ethylenedioxythiophene), polyaniline, polypyrrole, molecularly imprinted polymers, and others. In addition, a hybrid of the electrochemical biosensors and other techniques was highlighted. The outlook was also briefly discussed for the development of CP composite-based electrochemical biosensors for POC diagnostic devices.

Inducing Cytotoxicity in Colon Cancer Cells and Suppressing Cancer Stem Cells by Dolasetron and Ketoprofen through Inhibition of RNA Binding Protein PUM1.

Clinical trials of new drugs often face a high failure rate of approximately 45 percent due to safety and toxicity concerns. Repurposing drugs with well-established safety profiles becomes crucial in addressing this challenge. Colon cancer ranks as the third most prevalent cancer and the second leading cause of cancer related mortality worldwide. This study focuses on the RNA-binding protein pumilio1 (PUM1), a member of the PUF family involved in post-transcriptional gene expression regulation. By utilizing molecular docking techniques and FDA-approved drugs, potential inhibitors against PUM1 were identified. Notably, dolasetron and ketoprofen demonstrated promising results, exhibiting strong binding affinity, hydrophobic interactions, and favorable chemical reactivity according to Conceptual-DFT calculations. Both compounds effectively reduced cell viability, with IC50 values of 150 µM and 175 µM, respectively and shows long term inhibitory effects as seen by reduced in number of colonies. Moreover, they exhibited inhibitory effects on colon cancer stem cells, as indicated by reduced colonospheroid size and numbers. Apoptosis is induced by these compounds and has triggered activation of executioner caspase 3/7 in HCT116 cells which is evident through a caspase 3/7 assay and AO/EB staining, while the non-toxic effect of these compounds was evident from viability against non-cancerous cell line and hemolysis assay. Additionally, the treatment group showed a significant decrease in PUM1 and cancer stem cell markers expression compared to the control group. In conclusion, this study highlights the potential of targeting PUM1 as a novel approach to colon cancer treatment. Dolasetron and ketoprofen demonstrate promise as effective anti-cancer and anti-cancer stem cell drugs, inducing apoptosis in colon cancer cells through inhibition of PUM1.

Toxicity assessment of bioplastics on brine shrimp (Artemia franciscana) and cell lines

Polylactic acid (PLA) and polybutylene succinate (PBS), are categorized as biodegradable bioplastics that have progressively being utilized as food packaging replacing the obtrusive petroleum plastic. This study aims to evaluate the toxicity of bioplastics and petroleum-based plastic polyethylene (PE) on Artemia franciscana and animal cell lines. Each plastic sample was assessed in its powdered form, and the size distribution of PE, PLA, and PBS microplastic particles was observed by optical microscopy. Additionally, the toxicity of hatching percentage and mortality rate was investigated. The results demonstrated that the percentage of hatching and the mortality rate post treatment with PLA and PBS particles for 24 and 48 h did not significantly alter in comparison to those treated with PE particles. However, the percentage of Artemia hatching reduced post treatment with plastic particles in comparison to the control, as well as the mortality rate increased with a high concentration of plastic particles. The morphology of Artemia after ingesting microplastic particles was obtained using phase contrast inverted microscopy. The accumulation of plastic in the Artemia gut was observed succeeding its exposure to PE, PLA, and PBS particles. In addition, the cytotoxicity of plastics on human keratinocyte (HaCaT), normal human dermal fibroblast (NHDF), and African green monkey kidney (Vero) cell lines were determined by MTT assay. The result exhibited that increasing concentrations of microplastic particles had substantially less toxic to practically nontoxic effect on the cells. The biomolecule profile by synchrotron-IR technique displayed that plastics potentially have more alteration effect in epithelial kidney cells (Vero) than fibroblasts (NHDF) and keratinocytes (HaCaT). Our results demonstrate that PE, PLA, and PBS had toxicity on Artemia following ingestion, in accordance with the concentration and duration tests. Moreover, microplastic particles were nontoxic or harmless to animal cell lines and altered some intracellular biomolecule profiles. These discoveries are essential not only for environmental health risk assessment but also in order to establish recommendations toward safer utilization of bioplastics-based materials for a robust lifestyle.

Innovative Eco-Friendly Microwave-Assisted Rapid Biosynthesis of Ag/AgCl-NPs Coated with Algae Bloom Extract as Multi-Functional Biomaterials with Non-Toxic Effects on Normal Human Cells.

Harmful algal blooms impact human welfare and are a global concern. Sargassum spp., a type of algae or seaweed that can potentially bloom in certain regions of the sea around Thailand, exhibits a noteworthy electron capacity as the sole reducing and stabilizing agent, which suggests its potential for mediating nanoparticle composites. This study proposes an eco-friendly microwave-assisted biosynthesis (MAS) method to fabricate silver nanoparticles coated with Sargassum aqueous extract (Ag/AgCl-NPs-ME). Ag/AgCl-NPs-ME were successfully synthesized in 1 min using a 20 mM AgNO3 solution without additional hazardous chemicals. UV-visible spectroscopy confirmed their formation through a surface plasmon resonance band at 400-500 nm. XRD and FTIR analyses verified their crystalline nature and involvement of organic molecules. TEM and SEM characterization showed well-dispersed Ag/AgCl-NPs-ME with an average size of 36.43 nm. The EDS results confirmed the presence of metallic Ag+ and Cl- ions. Ag/AgCl-NPs-ME exhibited significant antioxidant activity against free radicals (DPPH, ABTS, and FRAP), suggesting their effectiveness. They also inhibited enzymes (tyrosinase and ACE) linked to diseases, indicating therapeutic potential. Importantly, the Ag/AgCl-NPs-ME displayed remarkable cytotoxicity against cancer cells (A375, A549, and Caco-2) while remaining non-toxic to normal cells. DNA ladder and TUNEL assays confirmed the activation of apoptosis mechanisms in cancer cells after a 48 h treatment. These findings highlight the versatile applications of Ag/AgCl-NPs-ME in food, cosmetics, pharmaceuticals, and nutraceuticals.

An Estimation of the Antiviral Activity and Toxicity of Biologically Active Substances Obtained from the Raw Materials of Artemisia cina Berg. In Vitro and In Vivo.

Species of the genus Artemisia are well known for their use as ingredients in ancient medicine. The advantage of using plant extracts compared to individual pharmaceutical ingredients is the rate of adaptation of the pathogenic microorganisms to the drug. Due to the rapid development of multidrug-resistance in microorganisms in the field, it is essential to search for novel, effective drugs with low toxicity. Therefore, the purpose of this study was to isolate and study the biologically active substances obtained from various substances in the raw materials of Artemisia cina Berg. The identification of the main biologically active components was performed using the method of chromato-mass spectrometry. Moreover, the antiviral activity of several extracts was studied using the method of measuring limiting dilutions (the Reed-Mench method), with some modifications. For the first time, the biological activity of extracts from the raw material of Artemisia cina Berg. upon the SARS-CoV-2 virus was confirmed. All the obtained extracts exhibited nontoxic effects in animals, with an LD50 greater than 2 g/kg. Comprehensive toxicological analyses are also presented in the study, such as those of the biochemical parameters of urine after one day and one week of the extracts' administration in mice at a dose of 2 g/kg body weight. In all groups of animals that received extracts of Artemisia cina Berg., a slight increase in the presence of red blood cells in their urine was observed one day following the administration of the extracts. This increase decreased somewhat after a week; however, it remained higher than the levels observed in the control animals. In the three groups, there was also a slight increase in the amount of ketones in the urine. Two weeks following the administration of the extracts to these groups, the internal organs of the animals were examined. The examination showed that the internal organs of the animals that received the extracts were not visibly different from those of the control animals in terms of their size or appearance. The weight of the internal organs of the animals that received the extracts was also similar to the weight of the internal organs of the control animals, illustrating the absence of toxicity.

Anticancer effects of herbal medicine Emelia-M, Mshikazi and Delosma H against human leukaemia cells.

Leukaemia is one of the three major types of blood cancers that lead to the overproduction of abnormal white blood cells. Emelia M (EMB), Mshikazi and Delosma H are herbal medicines that are being used by traditional healers in KwaZulu-Natal, South Africa to treat leukaemia and other diseases. To gain insight into the safety (non-toxic effect), anti-cancer activity, mechanisms of action and phytochemical profiles of traditional herbal medicines (Emelia M (EMB), Mshikazi and Delosma H) in South Africa. The viability of human peripheral blood mononuclear cells (PBMCs), monocytic (THP-1) and T-lymphocyte (Jurkat) cell lines exposed to varying concentrations of aqueous extracts of the three herbal medicines were assessed using adenosine triphosphate (ATP) assay. All three extracts showed a dose-dependent effect on the viability of PBMCs. Cell viability decreased with increasing concentrations of extracts when compared with the untreated cells at 24 and 48 hours. The inhibitory activities (IC50) of the extract were found in the order of Mshikazi > EMB, > Delosma H. All the extracts induced apoptosis with minimal necrosis. Many bioactive compounds that have been previously reported to have anticancer effects were identified in the extracts. Mshikazi extract significantly inhibited the growth of THP-1 and Jurkat cells and induced cell death through apoptosis than the other two extracts.

Meta-Analysis: Effect of Acupuncture Therapy in Lowering Frozen Shoulder Pain

Background: Acupuncture therapy has become a popular non-drug therapy with good curative effects, convenient application, non-toxic side effects, and low cost, and it has been widely used in the treatment of Frozen Shoulder. This study aims to analyze and estimate the effect of acupuncture therapy on reducing the degree of pain in Frozen shoulders. Subjects and Method: This study used a systematic review and meta-analysis by following PICO, Population: Frozen Shoulder patients. Intervention: acupuncture therapy. Comparison: no acupuncture therapy. Result: reduction in the degree of Frozen shoulder pain. The data used was obtained from scientific research articles from electronic databases including PubMed, Google Scholar, Hindawi, BMC, Scopus, and Science Direct Randomized Control Trial (RCT) designs from 2011 to 2022 which report effect sizes with mean and SD. The keywords used in the search for scientific articles are “Acupuncture” AND “Frozen Shoulder” AND “Frozen Shoulder Pain”. The selection of articles was carried out using the PRISMA flowchart. Data were analyzed using Review Manager software version 5.4.1. Results: A total of 9 articles with eligible RCT designs from Asia and Europe were selected for the systematic review and meta-analysis. The results of the meta-analysis showed that patients with Frozen Shoulders who received acupuncture therapy experienced a degree of pain 0.48 units lower than without acupuncture and was statistically significant (SMD= -0.48; 95% CI= -1.14 to -0.18; p=0.15). Conclusion: Acupuncture therapy reduces the degree of Frozen Shoulder pain. Keywords: acupuncture, pain, frozen shoulder. Correspondence: Mehdya Vikia Murti. Masters Program in Public Health, Universitas Sebelas Maret. Jl. Ir. Sutami 36A, Surakarta, Central Java 57126. Email: mehdyvikiamurti@gmail.com. Mobile: +62895363037033.

Toxicity test of bioceramic biphasic calcium phospate (BCP) Sr-Ag doping as bone graft in BHK-21 fibroblast cells

Background: Bone graft is a substitute material that is used to assist reconstruction, stabilize the structure and bonds in bone, stimulate the osteogenesis process and as a healing bone defect. One type of bone graft that has good osteoinductive and bicompatibility is alloplast which is a synthetic calcium phosphate compound. The most frequently used Calcium Phosphate groups are Hydroxyapatite (HA), β-Tricalcium Phosphate (β-TCP), and Biphasic Calcium Phosphate (BCP). In this study the material used was BCP doping Sr2+ and Ag+. Strontium ions (Sr2+) can increase osteoblast activity, reduce osteoclast activity and cytokine production, improve osteointegration, and minimize fractures. Ag+ ion has the ability as an antibacterial agent. Purpose: To explain and prove the toxicity of bioceramic Biphasic Calcium Phosphate (BCP) doped Sr-Ag as bone graft on BHK-21 fibroblast cells. Methods: This type of research is a laboratory experiment with a post-test only control group design. Treatment with Biphasic Calcium Phosphate (BCP) doped Sr-Ag with concentrations of 200 ppm, 180 ppm, 160 ppm, 140 ppm, 120 ppm, 80 ppm, 40 ppm, 20 ppm, 10 ppm, 5 pmm, 2.5 ppm, 1.25 ppm, 0.625 ppm in BHK-21 fibroblast cell culture. Results: The percentage of fibroblast cell life at concentrations of 200 ppm, 180 ppm, 160 ppm, 140 ppm, 120 ppm, 80 ppm, 40 ppm, 20 ppm, 10 ppm, 5 pmm, 2.5 ppm, 1.25 ppm, 0.625 ppm, respectively, the percentage of live cells was 38% 44%, 46%, 50%, 52%, 65%, 69%, 71%, 72%, 75%, 77%, 81%, and 87%. The parameter used in this toxicity test is CD50. Conclusion: The results of the toxicity test of bioceramic Biphasic Calcium Phosphate (BCP) doped Sr-Ag as a bone graft showed a toxic and non-toxic effect on BHK-21 fibroblast cells at certain concentrations.

Effects of Sihuang Zhili Granules on the Diarrhea Symptoms, Immunity, and Antioxidant Capacity of Poultry Challenged with Lipopolysaccharide (LPS).

A growing interest has been focused on Chinese herbs as alternatives to antimicrobial growth promoters, which are characterized by non-toxic side effects and drug resistance. The purpose of this study was to evaluate the effects of the Sihuang Zhili granule (abbreviated as Sihuang) on diarrhea, immunity, and antioxidation in poultry. Thirty male Leghorn chickens, aged 21 days, were randomly assigned to one of three groups with ten animals each. The control group (CON) received intraperitoneal saline injections, while the LPS-challenged group (LPS) and Sihuang intervention group (SH) received intraperitoneal injections of LPS (0.5 mg/kg of BW) and Sihuang (5 g/kg) at d 31, d 33, d 35, respectively. The control and LPS groups were fed a basal diet, while the SH group was fed a diet supplemented with Sihuang from d 21 to d 35. Analysis of the diarrhea index showed that the addition of Sihuang inhibited the increase in the diarrhea grade and the fecal water content caused by LPS, effectively alleviating poultry diarrhea symptoms. The results of the immune and antioxidant indexes showed that Sihuang significantly reduced the contents of the pro-inflammatory factors TNF- α and IL-1 β, as well as the oxidative stress markers ROS and MDA. Conversely, it increased the contents of the anti-inflammatory factors IL-4 and IL-10, along with the activities of antioxidant enzymes GSH-Px and CAT, thereby enhancing the immune and antioxidant abilities of chickens. Furthermore, Sihuang protected the chicken's ileum, liver, and immune organs from LPS invasion and maintained their normal development. In conclusion, this study confirmed the antidiarrheal effect of Sihuang in poultry farming and demonstrated its ability to improve poultry immunity and antioxidant capacity by modulating antioxidant enzyme activity and inflammatory cytokine levels.

Characterization of Secondary Metabolites and Cytotoxic Assay of Haliclona sp. Sponge Against T47D Breast Cancer Cells

Characterization of secondary metabolites and cytotoxic testing of Haliclona sp. against T47D breast cancer cells were conducted in this study. The objective was to assess the cytotoxicity of T47D cancer cells and identify the functional groups involved. The research methods employed included maceration, partitioning, phytochemical testing, toxicity testing using the BSLT method, separation through flash column chromatography (FCC), cytotoxic testing using the MTT method, and characterization using FTIR. The partition results of methanol extract consist of n-hexane, ethyl acetate, and methanol-water fractions. The methanol extract demonstrated high toxicity, with an LC50 of 5.21 ppm. Among the fractions, the ethyl acetate fraction exhibited the highest toxicity compared to the n-hexane and methanol-water fractions, with LC50 values of 25.76 ppm, 42.71 ppm, and 55.26 ppm, respectively. Phytochemical testing of the ethyl acetate fraction yielded positive results for terpenoids, steroids, alkaloids, and phenolic compounds. The ethyl acetate fraction was further separated using flash column chromatography, resulting in ten combined fractions (M1-M10). The cytotoxicity tests of the M3 fraction against 747D breast cancer cells showed non-toxic effects, with an IC50 value of 1382.29 ppm. The FTIR analysis of the M3 fraction revealed the presence of functional groups such as O-H, =C-H, C-H aliphatic, C=O, and C=C, which is indicative of the presence of terpenoids, steroids, and esters.

Effect of iron-fortified jamun leather on the Asunra-induced anemia in Sprague Dawley rats.

Micronutrients such as minerals and vitamins are required in a minute quantity but play a pivotal role in the functioning of the body. Therefore, deficiency in one of them can lead to lethal health conditions. Iron deficiency anaemia is one of the most common micronutrient deficiencies across the world and is affecting women and children. The present study aimed to investigate the anti-anaemic effect of fortified jamun leather on anaemia biomarkers and haematology in anaemic female Sprague Dawley rats. A total of 40 Sprague Dawley rats were used in 4 groups. Iron deficiency anaemia was induced by oral administration of the Asunra drug. The treatments were fed at two dosage levels i.e., 40 and 60% iron-fortified leather. All animals were treated for 60 days and the parameters including biochemical, and histopathology of the kidney and liver were examined. The experiment's findings showed that the group fed with iron-fortified leather (G3) succeeded significantly (P < 0.05) in restoring the serum iron (98.68 ± 2.88 μg/dL), haemoglobin (12.41 ± 0.32 g/dL), ferritin (24.54 ± 1.98 ng/mL) and haematocrit levels (39.30 ± 1.66%) at the end of the 60 days period. Additionally, the treated group's mean values for transferrin and total iron binding capacity were lower than those of the anaemic rats, indicating an improvement in iron levels. The microscopic analysis revealed that treatments had no toxic effects on the kidney and liver tissues, except in the diseased group, which had necrosis and irregular cell structure. Conclusively, iron-fortified jamun leather helped improve iron deficiency biomarkers and imparted a non-toxic effect on tissues in rats.

Interaction mechanism of egg derived peptides RVPSL and QIGLF with dipalmitoyl phosphatidylcholine membrane: microcalorimetric and molecular dynamics simulation studies.

Egg-derived peptides are becoming increasingly popular due to their biological activity and non-toxic effects. The egg-derived peptides Arg-Val-Pro-Ser-Leu (RVPSL) and Gln-Ile-Gly-Leu-Phe (QIGLF) display strong angiotensin-converting enzyme inhibitory activity and they can be taken up by intestinal epithelial cells. The interaction of the egg-derived peptides RVPSL and QIGLF with the membrane remains unclear. The position and structure of the peptides in the membrane were calculated. The maximum density values of RVPSL and QIGLF were 2.27 and 1.22 nm from the center of the 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) membrane, respectively, indicating that peptides penetrated the membrane-water interface and were embedded in the membrane. The interaction of RVPSL and QIGLF with the DPPC membrane did not affect the average area per lipid or the lipid sequence parameters. The thermodynamic parameters ΔH, ΔG, and ΔS of the interaction between the peptide RVPSL with the DPPC membrane were 17.91 kJ mol-1 , -17.63 kJ mol-1 , 187.5 J mol-1 ·k-1 , respectively. The thermodynamic parameters ΔH, ΔG, and ΔS of the interaction between peptide QIGLF with DPPC membrane were 17.10 kJ mol-1 , -17.12 kJ mol-1 , 114.8 J mol-1 ·k-1 , respectively. The results indicated that the binding of peptides RVPSL and QIGLF to DPPC is an endothermic, spontaneous, and entropy-driven reaction. The results of the study are relevant to the problem of the low bioavailability of bioactive peptides (BP). © 2023 Society of Chemical Industry.

Antimicrobial evaluation of Tripleurospermum callosum (Boiss. & Heldr.) E. Hossain extracts using in vitro and in vivo Caenorhabditis elegans model against urinary system pathogens

ABSTRACT Tripleurospermum callosum (Boiss. & Heldr.) E. Hossain was recorded in Turkish ethnobotanical data for its use against urinary and respiratory system ailments. Infusion, decoction and 96% ethanol extracts of T. callosum aerial parts were prepared for in vitro antimicrobial activity against urinary system pathogens Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027, Klebsiella aerogenes ATCC 1348 and Candida albicans ATCC 10231. The non-toxic concentrations of extracts and in vivo antimicrobial assay were performed using C. elegans. The extracts were analysed by Liquid Chromatography Mass Spectrometry (LC-MS/MS) for phytochemical composition. The water extracts were non-toxic at between 5000 and 312 µg/mL, while 96% ethanol extract at 312 µg/mL for C. elegans. The infusion extract showed in vivo anti-infective effect 5000–312 μg/mL against Gram-negative strains. The results indicate a potential role of plant extracts with relatively non-toxic and anti-infective effects against urinary system pathogens.

Toxicological Study of n-hexane and Chloroform Extracts of Lantana camara L. in Experimental Animals

Abstract: Aim: The aim of the current study is to investigate the acute, sub-acute and sub-chronic toxicity study of extracts (n-hexane and chloroform) of L. camara in Wistar rats. Materials and Methods: HPTLC and GC-MS analysis were carried out to analyze different constituents present in L. camara extract. Acute toxicity study was conducted as per OECD 420 guidelines, while sub-acute and sub-chronic studies were conducted according to OECD 407 and 408 guidelines respectively. Toxicopathological effects of both extracts were tested with the help of hemotoxylin and eosin staining of vital organs (liver, kidney, brain, testes, and ovaries) in experimental animals. Antioxidant and behavioral tests were also conducted on both the extracts, so as to evaluate the possible effects of toxicity. Results: The toxicity investigation of chloroform and n-hexane extracts exhibits no toxicity in terms of hematological (p<0.01) and biochemical parameters (p<0.05). Histopathological sections also confirmed the non-toxic effects on the liver, brain, kidney and on reproductive organs. Conclusion: The n-hexane and chloroform extracts of L. camara can be safely used for therapeutic purposes. Keywords: Lantana camara, GC-MS, HPTLC, Toxicity, Histopathology, Antioxidant, PI uptake.