CorrigendumCorrigendumPublished Online:01 Mar 2013https://doi.org/10.1152/ajpendo.zh1-6771-corr.2013Original articleMoreSectionsPDF (3 MB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInEmail Volume 303, January 2013Xiao X, Mruk DD, Cheng CY. c-Yes regulates cell adhesion at the apical ectoplasmic specialization-blood-testis barrier axis via its effects on protein recruitment and distribution; doi:10.1152/ajpendo.00422.2012; http://ajpendo.physiology.org/content/304/2/E145.full.In this article, in Fig. 4E, image b, part 1 was errorously placed on the top part of the image (the images are duplicates of each other). The correct version of Fig. 4 is listed here. The error and correction make no change in the results or conclusions discussed in the article.Fig. 1.Changes in the distribution of BTB integral membrane proteins and actin regulatory protein Eps8 and F-actin organization following in vivo c-Yes knockdown in adult rat testes. Frozen sections of rat testes obtained 2 days after administration of the last dose of siRNA duplexes for c-Yes knockdown in vivo in adult rats at 60–70 days of age (∼250–300 g body wt) vs. nontargeting control siRNA duplexes were stained for BTB integral membrane proteins occludin and N-cadherin (C and D) as well as F-actin and the actin-bundling and barbed-end capping protein Eps8 (E and F). Cell nuclei were visualized with DAPI (blue). Areas boxed by white rectangles in A, images a and b, and C and E, images a–d, were magnified to better illustrate changes in protein localization at the BTB and shown as 1 and 2 in A, C, and E, which were analyzed for either changes in fluorescence intensity or mislocalization by diffusing away from the basement membrane (BM), as shown in B, D, and F. Dashed lines mark the relative location of the BTB near the basement membrane in images a–d. Areas boxed by green rectangles in images b and d from the c-Yes RNAi group were enlarged and are shown to the right of images b or d with white arrowheads to indicate that the elongating/elongated spermatids there were embedded in the seminiferous epithelium vs. control tubules of the same stages, such as in stage VIII of the epithelial cycle, showing defects in spermiation, some of which were misoriented/misaligned, losing their polarity with the heads no longer pointing toward the basement membrane (see yellow arrowheads). The silencing of c-Yes was also confirmed in these tubules in which c-Yes immunostaining was diminished considerably (A, image b2 vs. image a2), and the c-Yes staining intensity was diminished by ∼75% (B), consistent with findings shown in Fig. 1, A and B. The distribution of occludin was also found to be altered and mislocalized, and it was no longer tightly restricted to the BTB (as denoted by 2 white arrows in C, image b2 vs. image a2), consistent with findings shown in Fig. 1. N-cadherin (C, image d2 vs. image c2, and D), F-actin (E, image b2 vs. image a2, and F), and Eps8 (E, image d2 vs. image c2) also displayed a considerably diffused pattern of distribution at the BTB (see white brackets). Semiquantitative analysis of densitometric data, such as changes in staining intensity (e.g., c-Yes and occludin) and protein mislocalization, where a target protein (e.g., N-cadherin, F-actin, Eps8) that moved away from the BM was summarized in B, D, and F, with data in the nontargeting control group arbitrarily set at 1. Each bar is the mean ± SD of ∼50 seminiferous tubules from 3 different rats (**P < 0.01). Scale bar in image a = 100 μm, which applies to images b–d. Scale bar in 1 = 30 μm, which applies to 2 and also micrographs in green boxes.Download figureDownload PowerPointThis article has no references to display. Previous Back to Top FiguresReferencesRelatedInformationRelated articlesc-Yes regulates cell adhesion at the apical ectoplasmic specialization-blood-testis barrier axis via its effects on protein recruitment and distribution 15 Jan 2013American Journal of Physiology-Endocrinology and Metabolism More from this issue > Volume 304Issue 5March 2013Pages E555-E556 Copyright & PermissionsCopyright © 2013 the American Physiological Societyhttps://doi.org/10.1152/ajpendo.zh1-6771-corr.2013History Published online 1 March 2013 Published in print 1 March 2013 PDF download Metrics Downloaded 103 times