Non-prolific Ewes Research Articles

PSVII-11 Genetic parameter estimates for lamb survival in prolific and nonprolific ewes

Abstract Given the increasing global demand for animal products, increasing lamb survival is a key breeding objective for sheep. However, high prolificacy can be associated with increases in fetal and lamb mortality. Therefore, the goal of this study was to estimate the variance components and genetic parameters for lamb survival (LS), using a: 1) single-trait model, where all ewes are analyzed together; and 2) two-trait model, where ewes are grouped in two groups based on birth type [i.e., Group 1 (G1) = single lamb/ewe, and Group 2 (G2) = multiple lambs/ewe]. In both models, LS was defined as the survival of lamb from birth to weaning and analyzed as a threshold trait. A total of 1,040,629 records (215,075 from single and 825,554 from multiple birth) from 319,467 ewes from several breeds/breed groups were available for the study. There were no females with two different types of births. Variance components were estimated through Bayesian inference via Gibbs sampling implemented in the GIBBSF90+ program, fitting a two-trait animal model with maternal effects, in which breed/breed groups were included as fixed effects. Average number of lambs/ewe in G2 was 2.57 (SD = 0.72). Heritability estimated (± SE) for LS on the underlying scale using the single-trait (0.04 ± 0.10) and two-trait model (0.010 ± 0.001 for LSG1 and 0.031 ± 0.004 for LSG2) were low. Maternal heritability estimated by the single-trait model was 0.007 ± 0.043, while by the two-trait model the estimates were 0.010 ± 0.006 and 0.040 ± 0.005 for LSG1 and LSG2, respectively. The genetic correlation estimated between LSG1 and LSG2 was weak and positive (0.25 ± 0.21). The low non-significant genetic correlation between single and multiple birth groups suggests that lamb survival is a different genetic trait in different birth types. Therefore, selecting for improving LS in nonprolific ewes may not necessarily translate to improving LS in prolific ewes. This underscores the need for careful consideration and targeted breeding strategies to achieve desired outcomes in nonprolific and prolific sheep simultaneously. Further studies to define the optimal statistical approach to genetically evaluate LS in prolific and nonprolific ewes are warranted.

Appearances of Local Prolific Ewes in Semarang Regency Central Java Indonesia

The present study examined the quantitative and qualitative physical parameters of prolific local ewes in Bawen and Jambu districts, Central Java. We used purposive sampling to select and categorized 132 local, three-lambing ewes into three groups based on their prolific capacity: 66 single, 49 twins and 17 triplets. The data were subjected to analysis of variance using Chi-Square. The result showed that many quantitative properties of prolific ewes resembled those of fat-tailed sheep: thin tail, highly significant (P<0.01) compared to fat tails; straight face, highly significant (P<0.01); and fewer convex face and short body, significant (P<0.05) with a tall body in the non-prolific ewes. Few prolific ewes had big ears and black-white wool and significantly different (P<0.05) from the non-prolific ewes. Conclusively, local prolific ewes had a qualitative appearance more closely related to that of thin-tailed ewes, but greater quantitative performances than the non-prolific ewes.

Is progesterone the key regulatory factor behind ovulation rate in sheep?

Ovarian antral follicles in the ewe grow in an orderly succession, producing 3 to 4 waves per estrous cycle. In prolific sheep, some large antral follicles from the second-to-last wave of the estrous cycle are added to the ovulatory follicles emerging just before estrus to give a higher ovulation rate; it is feasible that regression of these follicles is prevented by an increase in serum concentrations of FSH or LH pulsatility at proestrus. Prolific sheep tend to have a shorter luteal phase than nonprolific ewes and there is a great deal of evidence that luteal progesterone (P4), in addition to regulating LH release, may govern the secretion of FSH heralding the emergence of follicular waves. The specific purpose of this study was to determine whether or not extending the duration of the luteal phase in prolific sheep to that typically seen in nonprolific breeds would alter the follicle wave dynamics and ovulation rate. In 2 separate experiments, exogenous P4 (7.5 mg per ewe intramuscularly) was administered on day 11 at PM and day 12 at AM (day 0 = first ovulation of the interovulatory interval studied) in moderately prolific Rideau Arcott × Polled Dorset ewes (experiment 1, n = 8) and highly prolific Olkuska ewes (experiment 2, n = 7; TRT), whereas the equinumerous groups of animals served as controls (CTR). Transrectal ovarian ultrasonography was performed daily, and jugular blood samples were drawn twice a day from day 9 until the next ovulation. Progesterone injections resulted in relatively uniform increments in serum P4 levels, but the mean duration of the interovulatory interval did not differ (P > 0.05) between TRT and CTR groups of ewes in either experiment. The mean ovulation rate post-treatment was 1.6 ± 0.2 vs 3.2 ± 0.4 (experiment 1, P < 0.001) and 3.2 ± 0.8 vs 4.0 ± 1.0 (experiment 2, P > 0.05) in TRT vs CTR, respectively. The number and percentage of ovulating follicles from the penultimate wave of the interovulatory interval studied was 0.25 ± 0.16 vs 1.75 ± 0.45 (P < 0.01) and 25.0 ± 16.4% vs 75.0 ± 16.4% (P < 0.05) in experiment 1, and 0.50 ± 0.30 vs 1.60 ± 0.40 (P < 0.05) and 13.8 ± 9.0% vs 53.4 ± 16.7% (P < 0.05) in experiment 2, for TRT vs CTR, respectively. In summary, administration of P4 at the end of diestrus decreased the incidence of ovulations from the penultimate wave of the estrous cycle in both the moderately and highly prolific strains of sheep, but it reduced the ovulation rate only in moderately prolific ewes.

Correlations between ultrasonographic characteristics of corpora lutea and systemic concentrations of progesterone during the discrete stages of corpora lutea lifespan and secretory activity in cyclic ewes

Associations between physical characteristics and functionality of corpora lutea (CL) have previously been reported in monovulatory species, albeit several studies in cattle and humans have refuted the existence of temporal relationships between CL size, echotexture and serum progesterone (P(4)) concentrations. The main objective of the present study was to examine whether or not there were correlations between ultrasonographic image attributes of CL and systemic concentrations of P(4) during the discrete stages of the luteal phase in two breeds of sheep differing in ovulation rates (non-prolific Western White Face [WWF] ewes and prolific Finn [F] sheep). Transrectal ovarian ultrasonography utilized a 7.5-MHz linear-array transducer connected to a portable scanner (Aloka SSD-500) and the images were analyzed using commercially available image analytical software (Image ProPlus(®)) validated for the present application in sheep. The correlations were assessed using the Pearson's Product Moment (PPM) analysis and also, to increase the accuracy of statistical tests, the analysis of covariance (ANCOVA), with the number of CL as a co-factor. In WWF ewes, serum concentrations of P(4) correlated significantly with the total luteal area (TLA) during the CL growth phase (days 3-6; day 0 = ovulation) and functional luteolysis (days 12-15), and with numerical pixel values (NPVs--pixel intensity) during luteolysis; the results obtained by using two different statistical methods were generally similar. In prolific F ewes, serum P(4) concentrations were directly correlated with TLA during CL growth (days 3-6; ANCOVA), functional luteolysis (days 13-14; PPM), and structural CL regression (days 11-14; PPM and ANCOVA), and with NPVs during functional luteolysis (PPM and ANCOVA). We concluded that systemic P(4) concentrations could only be accurately predicted from the changes in luteal area during CL growth and regression, and from NPVs during luteolysis, in both prolific and non-prolific ewes, but the changes in size and echotexture of the luteal glands at mid-cycle were not indicative of serum P(4) concentrations in sheep.

Effects of dietary supplements of folic acid on reproductive performance in ewes

The objective of this project was to assess the impact of periconceptional folic acid supplementation on the reproductive performance of prolific and non-prolific ewes, in the estrous and anestrous seasons. Two initial trials took place during the estrous season at two experimental sites where 38 Dorset and 39 half-Finn half-Dorset ewes (site A) as well as 80 Dorset ewes (site C) were divided into two groups receiving either 0 or 210 mg ewe-1 d-1 of folic acid over a period which extended from 21 d premating to 30 d postmating. Three other trials were conducted in the anestrous season where 80 Dorset ewes (site A), 56 half-Romanov ewes (site B) and 78 Dorset ewes (site C) were subjected to the same protocol as the one used in the breeding season. In all the trials, the folic acid supplement increased plasma and red cell folates, but had no effect on fertility, embryonic mortality, or the size or weight of the litter at birth. Folic acid supplementation did not improve the reproductive performance of prolific and non-prolific ewes, either in the estrous season or in the anestrous period. Key words: Ewes, reproduction, vitamins, folic acid, fertility, prolificacy

Computer assisted image analyses of corpora lutea in relation to peripheral concentrations of progesterone: A comparison between breeds of sheep with different ovulation rates

Transrectal ovarian ultrasonography is a non-invasive technique that permits the real-time serial visualization of ovarian structures. Previous studies have reported correlations between serum progesterone concentrations and physical characteristics of the corpus luteum (CL) in different species. The aim of the present study was to investigate whether or not there were correlations between ultrasound image attributes of the CL and changing progesterone concentrations over time, in prolific and non-prolific ewes. Ultrasonographic images of CL were taken, once daily, from 12 Western White Face ewes and 7 Finn ewes for the duration of one luteal phase. Blood samples were collected daily prior to each scanning session and assayed to determine mean serum concentrations of progesterone. Analysis of ultrasound images was performed using a series of custom-developed computer algorithms optimized for ultrasonography on a computer graphics workstation. Both total luteal area and mean pixel values were correlated with the pattern of serum concentrations of progesterone from days 3 to 15 after ovulation in Western White Face ewes and from days 3 to 14 in Finn ewes. There was no significant correlation between progesterone concentrations and spot pixel heterogeneity for either Western White Face ewes or Finn ewes. We concluded that pixel heterogeneity is a poor indicator of progesterone secretory ability of the CL when compared to mean pixel values. However, luteal area and mean spot pixel values are better but not strong indicators of the functional status of the CL in cyclic ewes.

Effects of a 6-day treatment with medroxyprogesterone acetate after prostaglandin F2 alpha-induced luteolysis at midcycle on antral follicular development and ovulation rate in nonprolific Western white-faced ewes.

Medroxyprogesterone acetate (MAP) from intravaginal sponges prolongs the lifespan of large ovarian follicles when administered after prostaglandin F2alpha (PGF2alpha)-induced luteolysis early in the luteal phase of ewes. The present study was designed to determine whether a PGF2alpha/MAP treatment applied at midcycle would alter the pattern of antral follicle growth and increase ovulation rate in nonprolific ewes. A single injection of PGF2alpha (15 mg, i.m.) was given, and an intravaginal MAP (60 mg) sponge was inserted for 6 days, on approximately Day 8 after ovulation, in 7 (experiment 1), 8 (experiment 2) or 11 (experiment 3) ultrasonographically monitored, cycling Western white-faced ewes; seven ewes (experiment 1) served as untreated controls. Blood samples were collected each day and also every 12 min for 6 h, halfway through the period of treatment with MAP (experiment 1), or every 4 h, from 1 day before to 1 day after sponging (experiment 2). Seventeen of 26 treated ewes (experiment 1, n = 6; experiment 2, n = 5; experiment 3, n = 6) ovulated 1 to 6 days after PGF2alpha, but this did not affect the emergence of ensuing follicular waves (experiments 1 and 2). These ovulations, confirmed by laparotomy and histological examinations of the ovaries (experiment 3), were not preceded by an increase in LH/FSH secretion and did not result in corpora lutea, as evidenced by transrectal ultrasonography and RIA of serum progesterone (experiments 1 and 2). Following the removal of MAP sponges, the mean ovulation rate was 3.1 +/- 0.4 in treated ewes and 2.0 +/- 0.3 in control ewes (experiment 1; P < 0.05). In experiments 1 and 2, the ovulation rate after treatment (3.1 +/- 0.4 and 2.8 +/- 0.4) was also greater than the pretreatment rate (1.9 +/- 0.3 and 1.9 +/- 0.1, respectively). Ovulations of follicles from two consecutive waves before ovulation were seen in five treated but only in two control ewes (experiment 1), and in seven ewes in experiment 2. There were no significant differences between the MAP-treated and control ewes in mean daily serum concentrations of FSH and estradiol, and no differences in the parameters of LH/FSH secretion, based on frequent blood sampling. Treatment of nonprolific Western white-faced ewes with PGF2alpha and MAP at midcycle changed follicular dynamics and increased ovulation rate by approximately 50%. These effects of MAP, in the absence of luteal progesterone, may not be mediated by changes in gonadotropin secretion.