Non-organic Solvent Research Articles

Conversion of Nitrate to Ammonia by Amidinothiourea-Coordinated Metal Molecular Electrocatalysts with d-π Conjugation.

The electrochemical reduction of nitrate to ammonia (NO3RR) provides a desired alternative of the traditional Haber-Bosch route for ammonia production, igniting a research boom in the development of electrocatalysts with high activity. Among them, molecular electrocatalysts hold considerable promise for the NO3RR, suppressing the competing hydrogen evolution reaction. However, the complicated synthesis procedure, usage of environmentally unfriendly organic solvents, and poor stability of Cu-based molecular electrocatalysts greatly limit their employment in NO3RR, and the development of desired Cu-based molecular catalysts remains challenging. Herein, a simple nonorganic solvent involving a one-step strategy was proposed to synthesize d-π-conjugated molecular electrocatalysts metal-amidinothiourea (M-ATU). Cu-ATU is composed of Cu coordinated with two S and two N atoms, whereas Ni-ATU is formed by Ni with four N atoms from two ATU ligands. Remarkably, Cu-ATU with a Cu-N2S2 coordination configuration exhibits superior NO3RR activity with a NH3 yield rate of 159.8 mg h-1 mgcat-1 (-1.54 V) and Faradaic efficiency of 91.7% (-1.34 V), outperforming previously reported molecular catalysts. Compared to Ni-ATU, Cu-ATU transfers more electrons to the *NO intermediate, effectively breaking the strong sp2 hybridization system and weakening the energy of N═O bonds. The increase in free energy of *NO reduced the energy barriers of the rate-determining step, facilitating the further hydrogenation process over Cu-ATU. Our work opened up a new horizon for exploring molecular electrocatalysts for nitrate activation and paved a way for the in-depth understanding of catalytic behaviors, aligning more closely with industrial demands.

Formic acid stability in different solvents by DFT calculations.

New technologies have been developed toward the use of green energies. The production of formic acid (FA) from carbon dioxide (CO[Formula: see text]) hydrogenation with H[Formula: see text] is a sustainable process for H[Formula: see text] storage. However, the FA adduct stabilization is thermodynamically dependent on the type of solvent and thermodynamic conditions. The results suggest a wide range of dielectric permittivity values between the dimethyl sulfoxide (DMSO) and water solvents to stabilize the FA in the absence of base. The thermodynamics analysis and the infrared and charge density difference results show that the formation of the FA complex with H[Formula: see text]O is temperature dependent and has a major influence on aqueous solvents compared to the FA adduct with amine, in good agreement with the experiment. In these conditions, the stability thermodynamic of the FA molecule may be favorable at non-organic solvents and dielectric permittivity values closer to water. Therefore, a mixture of aqueous solvents with possible ionic composition could be used to increase the thermodynamic stability of H[Formula: see text] storage in CO[Formula: see text] conversion processes. Using the Quantum ESPRESSO package, density functional theory (DFT) calculations were performed with periodic boundary conditions, and the electronic wave functions were expanded in plane waves. For the exchange-correlation functional, we use the vdW-DF functional with the inclusion of van der Waals (vdW) forces. Electron-ion interactions are treated by the projector augmented wave (PAW) method with pseudopotentials available in the PSlibrary repository. The wave functions and the electronic densities were expanded employing accurate cut-off energies of 6.80[Formula: see text]10[Formula: see text] and 5.44[Formula: see text]10[Formula: see text] eV, respectively. The electronic density was computed from the wave functions calculated at the [Formula: see text]-point in the first Brillouin-zone. Each structural optimization was minimized according to the Broyden-Fletcher-Goldfarb-Shanno (BFGS) algorithm, with force and energy convergence criteria of 25 meV[Formula: see text]Å[Formula: see text] and 1.36 meV, respectively. The electrostatic solvation effects were performed by the [Formula: see text] package with the Self-Consistent Continuum Solvation (SCCS) approach.

An efficient dual-function fluorescent probe for sulfites and sulfides and its imaging application in cells

As gas signaling molecules in organisms, SO2 derivatives and H2S play crucial regulating roles in a series of physiological processes. Therefore, developing an assay that can accurately monitor the concentration of SO2 derivatives and H2S in cells is extremely important for the research and treatment of related illnesses. A bifunctional probe SN-F based on FRET mechanism for SO2 derivatives and H2S was designed. SN-F had a short response time to SO2 (2 min), excellent anti-interference capability and selectivity in the non-organic solvent system (pH = 7.4), which was suitable for the determination of SO2 derivatives in cells. SN-F had a wide linear range for H2S. Moreover, SN-F was applied in cell imaging successfully with high targeting ability to endoplasmic reticulum (ER) and could monitor endogenous and exogenous H2S in cells.

Food Wastes and Microalgae as Sources of Bioactive Compounds and Pigments in a Modern Biorefinery: A Review.

The United Nations 2030 Agenda for Sustainable Development has created more pressure on countries and society at large for the development of alternative solutions for synthetic and fossil fuel derived products, thus mitigating climate change and environmental hazards. Food wastes and microalgae have been studied for decades as potential sources of several compounds that could be employed in various fields of application from pharmaceutical to textile and packaging. Although multiple research efforts have been put towards extracting rich compounds (i.e., phenolic compounds, tocopherols, and tocotrienols) from these sources, they still remain overlooked as two major sources of bioactive compounds and pigments, mainly due to inefficient extraction processes. Hence, there is a growing need for the development of optimized extraction methods while employing non-organic solvent options following the main principles of green chemistry. This review will focus on delivering a clear and deep analysis on the existing procedures for obtaining bioactive compounds and pigments from food wastes derived from the most consumed and produced fruit crops in the world such as apples, oranges, cherries, almonds, and mangoes, and microalgal biomass, while giving light to the existing drawbacks in need to be solved in order to take full advantage of the rich properties present in these two major biorefinery sources.

Sustainable preparation of graphene-analogue boron nitride by ball-milling for adsorption of organic pollutants

The method of fabricating low-cost adsorbents with high activity and durability via a convenient and eco-friendly procedure is of great importance to wastewater treatment. Herein, a high-efficient mechanical exfoliation strategy was proposed to facilely prepare few-layered graphene-analogue boron nitride (BN) via a one-step non-organic solvent assisted wet ball mill procedure. Ball-milling treatment increased the specific surface area of BN 3.5-fold by reducing the thickness to ∼3 layers with 45 min. The exfoliated BN exhibited strikingly improved sorption performance to organic contaminants with around 124% and 116% increased removal efficiency respectively for oxytetracycline (OTC) and Rhodamine B (RhB) as compared to the bulk BN. Batches sorption experiments showed that the sorption processes were thermodynamic endothermic, and well fitted to pseudo-second-order kinetic model and Freundlich isotherm equation. The π-π stacking interaction, hydrophobic effect and electrostatic interaction were proposed as the dominated sorption mechanism. In addition, no significant decline in adsorptive removal ability for the sorbent after 5 times recycling. The results indicate that the ball-milling exfoliation is a fast, green, sustainable and promising strategy for synthesis of highly potent BN based two-dimensional layered adsorbents.

Drying and quality performance of dragon blood’s resin (Daemonorops sp.) exposed to solar and oven drying

Dragon blood’s resin is obtained by extracting the dragon blood’s fruit with organic or non-organic solvents. Following extraction, drying process is usually carried out to dry the wet resin. The drying techniques used is assumed to affect not only the drying performance but also the quality of the dragon blood’s resin, in particular its active ingredient content (dracorhodin). The purpose of this study was to measure and compare the effect of solar and oven drying on the drying performance and quality of dragon blood’s resin. The oven temperature used was 60°C. Both drying techniques were executed until the resin dried which was indicated by color change and its easiness to rupture. The results showed that solar drying of dragon blood required shorter time (210 minutes) with higher drying rate (0.25%/minute) than the oven drying process which need 250 minutes to finish with a drying rate of 0.14%/minute. Except the ash content of solar dried resin, other post-drying quality parameters of dragon blood resin from both drying techniques have met the Indonesia standard for trading purpose (SNI 8663:2018). Further statistical analysis confirmed that the drying techniques applied significantly affected the drying rate and post-drying quality values of dragon blood’s resin.

Controlled preparation and gas sensitive properties of two-dimensional and cubic structure ZnSnO3

Two-dimensional (2D) ZnSnO3 is a promising candidate for future gas sensors due to its high chemical response and excellent electronic properties. However, the preparation of 2D ZnSnO3 nanosheets by utilizing soluble inorganic salts and nonorganic solvents remains a challenge. In this work, 2D ZnSnO3 was synthesized via a facile graphene oxide (GO)-assisted co-precipitation method, in which inorganic salts in the aqueous phase replaced metal organic salts in a non-aqueous system. Meanwhile, a “dissolution and recrystallization” mechanism was proposed to explain the transformation from 3D nanocubes to 2D nanosheets. In comparison, the 2D ZnSnO3 nanosheets showed a higher response to formaldehyde (HCHO) at low operating temperature (100 °C). The response (Ra/Rg) of the 2D ZnSnO3 sensor to 10 ppm HCHO was as high as 57, which was approximately 5 times the response of the ZnSnO3 nanocubes sensor. However, the ZnSnO3 nanocubes sensor showed better gas sensing performance to ethanol at high temperature (200 °C). Different gas-sensitive properties were attributed to the different gas diffusion and adsorption processes caused by the morphology and nanostructure. Moreover, both sensors could detect either 0.1 ppm HCHO or ethanol at their optimum operating temperature. This work presents a relatively economical method to prepare 2D compound metal oxides, provides a novel “dissolution and recrystallization” mechanism for 2D multi-metal oxide preparation, and sheds light on the great potential of high-efficiency HCHO and/or ethanol gas sensors.

Novel chitosan-poly(vinyl acetate) biomaterial suitable for additive manufacturing and bone tissue engineering applications

Chitosan, a biocompatible and biodegradable natural polymer, offers great promise as a biomaterial for tissue engineering applications. Chitosan scaffolds have previously been fabricated using additive manufacturing techniques, however, the use of crosslinkers, weak mechanical stability and structural resolution remain problematic. In this study Chitosan-PVAc biopolymer blends were prepared using a non-organic solvent that can prepare a three-dimensional printable biopolymer in less time than conventional methods. Prepared films were characterised using SEM, FTIR and thermogravimetric analysis. Additionally, the swelling properties, biodegradability and printability of the scaffolds were also studied. The fabricated films were biodegradable within a 3-week period and showed controllable swelling properties. Results indicated no toxicity and cells attached onto films. Additionally, hydrogels showed antibacterial activity against S. aureus, S. epidermidis and E.coli, which could potentially prevent implant related infections. Additive manufacturing simulation of PVAc composite 3% chitosan and PVAc composite 4% chitosan were able to produce a layered scaffold without using crosslinkers and therefore confirming printability. Cytocompabability were assessed using a resazurin assay and cell attachment. From these results, we concluded that the printable PVAc composite 3% chitosan and PVAc composite 4% chitosan biopolymer blends meet the requirements of a biomaterial and can potentially be used for biomedical implants.

Water composed of reduced hydrogen bonds activated by localized surface plasmon resonance effectively enhances anti-viral and anti-oxidative activities of melatonin

Melatonin is a lipophilic antioxidant generally dissolved in organic solvent before delivery. However, the presence of organics may severely depress the functional effects of melatonin. By rendering deionized water (DIW) flow through gold nano-particles under localized surface plasmon resonant illumination, we developed plasmon-activated water (PAW) which successfully increases the solubility of melatonin to 150.325%. Melatonin dissolved in PAW also exhibits stronger anti-viral and anti-oxidative activities than that dissolved in DIW in which the percentage of dengue virus infected human hepatocellular carcinoma cells is remarkably decreased (14.7% vs. 20.6%) whilst the clearance rate of hydroxyl radical is significantly enhanced (11.9% vs. 6.69%), respectively. Moreover, in vivo approaches further show that following chronic sleep deprivation, the level of oxidative stress, hepatic bioenergetics, anti-oxidative enzyme activity, and metabolic function are all significantly improved in rats received melatonin prepared in PAW than that in DIW. As the bio-activity of melatonin depends largely on its solubility, utilizing PAW as a non-organic solvent will not only enhance the anti-viral and anti-oxidative function of melatonin, but also offer great potential for clinical use of melatonin as a therapeutic strategy to depress virus infection and counteract oxidative damage in a more natural, more economic and more efficient way.

An Actinobacterial Isolate, Streptomyces sp. YX44, Produces Broad-Spectrum Antibiotics That Strongly Inhibit Staphylococcus aureus.

The need for new antibiotics is increasing due to their overuse, and antibiotic resistance has become one of the major threats worldwide to public health, food safety, and clinical treatment. In this study, we describe an actinobacterial isolate, YX44, which belongs to the genus Streptomyces. This Streptomyces was isolated from a drinking pipe located in Osaka, Japan, and has the ability to inhibit Gram-positive bacteria, Gram-negative bacteria, and various fungi. YX44 fermentation broth shows strong activity against Escherichia coli and Staphylococcus aureus, as well as also inhibiting clinical isolates of multidrug-resistant Staphylococcus aureus. The YX44 antibacterial substances in the broth are relatively heat-stable, show high stability from the pH range 1 to 11, and have good solubility in both organic and non-organic solvents. Size-exclusion chromatography revealed that the YX44 antibacterial compounds are less than 1000 Da in size. LC-MS was able to identify three possible candidate molecules with molecular weights of 308, 365, 460, and 653 g/mol; none of these sizes correspond to any well-known antibiotics. Our results show that Streptomyces sp. YX44 seems to produce a number of novel antibiotics with high pH stability and good solubility that have significant activity against S. aureus, including multidrug-resistant strains.

Purification of xylooligosaccharides from bamboo with non-organic solvent to prepare food grade functional sugars

Food grade xylooligosaccharides (XOS) have a high application value, but most of the reported purification methods of XOS use organic solvents, which has significant problems such as large amount of solvent, residual solvent, and difficulty in solvent recovery. In addition, although there are many reported methods of separation and purification, quantitative analysis is lacking for each specific treatment step, such as sugar loss rate, decolorization rate, protein removal rate, and desalination rate. Therefore, purification of XOS from bamboo with non-organic solvent to prepare food grade functional sugars was presented by this study. What’s more, quantitative analysis and optimization was made for each step of separation, which was of great significance for guiding the actual production. Overall, through activated carbon adsorption, ion exchange and membrane filtration comprehensive treatments, food grade XOS were obtained from bamboo with 7.5% yield, a purity of 92.3%, and a total sugar proportion as high as 98.9%.

Processing, Carbonization, and Characterization of Lignin Based Electrospun Carbon Fibers: A Review

Greenhouse gas emissions and environmental impacts of petroleum-based fuels and materials have necessitated the development of renewable resource-based alternatives. In the process to extract cellulose for converting it to bioethanol (bio-based gasoline) large quantities of lignin are produced as the main byproduct-making it useful for further processing application for sustainable materials. Lignin is the second abundant source of renewable carbon with an aromatic structure which makes it a potential candidate for carbon fiber production. Since lignin can be dissolved in a variety of organic and non-organic solvents, electrospinning has been used to produce precursor fibers for carbon nanofiber production. These carbon nanofibers have been tested as a potentially sustainable alternative for the current non-renewable electrodes in energy storage and conversion devices such as supercapacitors. Using lignin by-products from the fuel energy sector in making devices for electrical energy sector provides a great opportunity for promoting a circular economy from sustainable materials while also contributing to researching alternative sustainable materials in light of a global pandemic. This review presents a summary of the processing conditions for electrospinning different varieties of lignin, characterization of the electrospun fibers and the carbonization conditions for converting fibers. Different techniques that of the structural properties of the precursor fibers, characteristics of carbon nanofibers and their performance in energy storage devices are discussed. Compared to the other published reviews in this field, this review aims to present the current knowledge on material-processing-lignin-carbon fibers properties relationship.

A Systematic Safety Evaluation of Nanoporous Mannitol Material as a Dry-Powder Inhalation Carrier System

For carrier-based dry-powder inhaler (DPI) formulations, the adhesion between carrier particles and active pharmaceutical ingredients (API) particles have a significant influence on the aerosolization performance of the API-carrier complexes and the desired detachment of the API for efficient pulmonary delivery. In our previous study, nanoporous mannitol material was successfully fabricated as carriers by a one-step nonorganic solvent spray drying method with the thermal degradation of ammonium carbonate. These carriers were shown to achieve excellent aerosolization performance. In addition, no residue of ammonium carbonate was detected on the powder surface. However, the safety of nanoporous mannitol carriers (Nano-PMCs) during pulmonary administration/delivery was still unknown because the lung is vulnerable to the inhaled particles. To address this question, the present study was conducted to construct a systematic safety evaluation for DPIs carriers to investigate the safety of Nano-PMCs in the whole inhalation, which would make up for the lack of detailed and standardized method in this field. In vitro safety evaluation was carried out using respiratory and pulmonary cytotoxicity tests, hemolysis assay, and ciliotoxicity test. In vivo safety evaluation was studied by measuring inflammatory indicators in the bronchoalveolar lavage fluid, assessing the pulmonary function and observing pulmonary pathological changes. Nano-PMCs showed satisfactory biocompatibility on respiratory tracts and lungs in vitro and in vivo. It was suggested that Nano-PMCs were safe for intrapulmonary delivery and potential as DPI carriers.

Interfacial polyelectrolyte complexation spinning of graphene/cellulose nanofibrils for fiber-shaped electrodes

Abstract

Self-Cleaning and Hydrophobic Pineapple Peel Fibre based Biocomposite

Biocomposite has been widely used as plastics replacement for its biodegradability. However, the water absorptivity defeats its purpose as food packaging. The water absorption analysis for pineapple peel fiber (PAPF) was done according to ASTM D570-98 and it has been proven that the water content increases to 7.2% with the 50% content of PAPF in the bio-composite (50% PAPF). For that matter, hydrophobic coating was synthesized and applied on PAPF based bio-composite. The bio-composite was produced using Low Density Polyethylene (LDPE), pineapple peel fibre (PAPF), Linear LDPE grafted maleic anhydride (LLDPE-g-MA) as compatibilizer and Refined, Bleached Deodorized Olein (RBDOL) as plasticizer. The coating was synthesized by using polydimethylsiloxane (PDMS) and decamethylcyclopentasiloxane as hydrophobic component, silica nano-particles as nanostructured particles and non-organic solvent and non-ionic surfactant p-octyl polyethylene glycol phenyl ether as surfactant. The emulsion was homogenized and after that sprayed onto the surface of PAPF bio-composite and dried in the oven at 50-60˚C for 24 hours. Through the 100µm magnification using field emission scanning electron microscope (FESEM), the hydrophobic coating was proven to conform the Cassie-Baxter hydrophobic property. C-O bond from the PAPF disintegrates after the production of bio-composite from the Attenuated Total Reflectance Fourier Transform Infrared (ATR FTIR) spectrometry transmittance. The application of the coating onto the surface of PAPF bio-composite gave the surface hydrophobic property where the contact angle changes from 82.27±2.66˚ which is hydrophilic to 122.63±2.17˚ which is hydrophobic. The bio-composite with the highest PAPF content will also exhibit self-cleaning ability which makes it suitable for the usage of hydrophobic food packaging material.

Determination of Anti-Diabetic Property of organic and nonorganic solvent extracts of Schizophyllum commune

Schizophyllum commune is well-known, non-consumable split gill mushroom having a variety of nutraceutical properties, due to the presence of various bioactive components. In this study, antidiabetic potency of Schizophyllum commune was evaluated using different extraction solvents. Results revealed the extract of methanol solvent showed high antidiabetic potency (IC50 =50.98 μg/ml), followed by another solvents such as ethanol and hot water extracts respectively. Antidiabetic activity of extracts was compared with standards and positive correlation was observed. In conclusion, based on accumulated data Schizophyllum commune may be used as natural antidiabetic substance and a better alternative to synthetic chemicals. It can be prospective source of potent constituent for fighting against human metabolic disorder.

Fluorescence Polarization Immunoassay for the Determination of T-2 and HT-2 Toxins and Their Glucosides in Wheat.

T-2 and HT-2 toxins and their main modified forms (T-2 glucoside and HT-2 glucoside) may co-occur in cereals and cereal-based products. A fluorescence polarization immunoassay (FPIA) was developed for the simultaneous determination of T-2 toxin, HT-2 toxin and relevant glucosides, expressed as sum. The developed FPIA, using a HT-2-specific antibody, showed high sensitivity (IC50 = 2.0 ng/mL) and high cross-reactivity (100% for T-2 toxin and 80% for T-2 and HT-2 glucosides). The FPIA has been used to develop two rapid and easy-to-use methods using two different extraction protocols, based on the use of organic (methanol/water, 90:10, v/v) and non-organic (water) solvents, for the determination of these toxins in wheat. The two proposed methods showed analytical performances in terms of sensitivity (LOD 10 µg/kg) recovery (92–97%) and precision (relative standard deviations ≤13%), fulfilling the criteria for acceptability of an analytical method for the quantitative determination of T-2 and HT-2 toxins established by the European Union. Furthermore, the methods were then validated in accordance with the harmonized guidelines for the validation of screening methods included in the Regulation (EU) No. 519/2014. The satisfactory analytical performances, in terms of intermediate precision (≤25%), cut-off level (80 and 96 µg/kg for the two methods) and rate of false positives (<0.1%) confirmed the applicability of the proposed methods as screening method for assessing the content of these toxins in wheat at the EU indicative levels reported for T-2 and HT-2 toxins.

Non-organic solvent prepared nanofiltration composite membrane from natural product tannic acid (TA) and cyclohexane-1,4-diamine (CHD)

Conventional nanofiltration membrane fabrication generally includes the usage of toxic organic solvent that poses threats to the environment and public health. Herein, we explored preparing nanofiltration membrane through a non-organic solvent method utilizing the highly reactive natural product, tannic acid (TA), along with cyclohexane-1,4-diamine (CHD). Fabrication process was carried out through sequential immersion of a polyethersulfone (PES) support in two aqueous monomer phases. The absence of a solvent interface facilitated the diffusion and mixing of the two monomers, and the highly reactive quinone derivative of TA enabled the rapid film formation via Michael addition and Schiff base formation. Membrane performance was optimized through investigation of fabrication parameters. The TA-CHD membrane exhibited a negatively charged surface, with a molecular weight cut-off (MWCO) of 600 Da. The membrane prepared at optimal conditions showed approximate 97% and 50.7% rejections towards Na2SO4 and NaCl, with a water flux at approximate 35 L m−2 h−1 (10 bar). Fouling behaviour of the TA-CHD membrane was studied and a chemical cleaning test under two extreme pH was performed to demonstrate the chemical stability. The proposed non-organic solvent method here provides an eco-friendly way to utilize the potential of polyphenol natural products in water purification membrane preparation.

Study of Acidified Aqueous Extraction of Phenolic Compounds from Hibiscus sabdariffa L. calyces

Introduction:Hibiscus calyces are important sources from anthocyanins and pigments. The recovery of these bioactive compounds using non-organic solvents becomes very attractive for the food industry.Methods:For this reason, the separation of phenolic compounds by acidified aqueous extraction from hibiscus calyces was studied. The experiments were conducted by a fractional factorial design.Result and Conclusion:Four factors were evaluated: temperature, time, stirring speed and enzyme concentration. The extracts produced were subjected to analysis of color (L*,a*,b*andChroma), total monomeric anthocyanins, antioxidant capacity by ABTS and fourteen phenolic compounds were quantified. The results showed that the best condition to obtain hibiscus calyces extract was using an enzyme concentration of 50 µL/1000 g hibiscus extract, 400 rpm of stirring speed at 55 ºC by 4 hours of extraction, that corresponded to concentrations of 17595, 7516, 2568 μg/g, expressed on a dry basis, for total phenolic compounds, delphinidin 3-sambubioside and cyanidin 3-sambubioside, respectively, and antioxidant capacity measured by ABTS of 7.8 µmol of Trolox equivalent per gram.

Protocol: a versatile, inexpensive, high-throughput plant genomic DNA extraction method suitable for genotyping-by-sequencing

BackgroundThe recent development of next-generation sequencing DNA marker technologies, such as genotyping-by-sequencing (GBS), generates thousands of informative single nucleotide polymorphism markers in almost any species, regardless of genomic resources. This enables poorly resourced or “orphan” crops/species access to high-density, high-throughput marker platforms which have revolutionised population genetics studies and plant breeding. DNA quality underpins success of GBS methods as the DNA must be amenable to restriction enzyme digestion and sequencing. A barrier to implementing GBS technologies is access to inexpensive, high-throughput extraction methods that yield sequencing-quality genomic DNA (gDNA) from plants. Several high-throughput DNA extraction methods are available, but typically provide low yield or poor quality gDNA, or are costly (US$6–$9/sample) for consumables.ResultsWe modified a non-organic solvent protocol to extract microgram quantities (1–13 μg) of sequencing-quality high molecular weight gDNA inexpensively in 96-well plates from either fresh, freeze-dried or silica gel-dried plant tissue. The protocol was effective for several easy and difficult-to-extract forage, crop, horticultural and common model species including Trifolium, Medicago, Lolium, Secale, Festuca, Malus, Oryza, and Arabidopsis. The extracted DNA was of high molecular weight and digested readily with restriction enzymes. Contrasting with other extraction protocols we assessed, Illumina-based sequencing of GBS libraries developed from this gDNA had very uniform high quality base-calls to the end of sequence reads. Furthermore, DNA extracted using this method has been sequenced successfully with the PacBio long-read platform. The protocol is scalable, readily automated without requirement for fume hoods, requires approximately three hours to process 192 samples (384–576 samples/day), and is inexpensive at US$0.62/sample for consumables.ConclusionsThis versatile, scalable and simple protocol yields high molecular weight genomic DNA suitable for restriction enzyme digestion and next-generation sequencing applications including GBS and long-read sequencing platforms such as PacBio. The low cost, high-throughput, and extraction of high quality gDNA from a range of fresh and dried source plant material makes this method suitable for many sequencing and genotyping applications including large-scale sample screening underpinning breeding programmes.