non-OA Group Research Articles

LncRNA SNHG5/miR-26a/SOX2 signal axis enhances proliferation of chondrocyte in osteoarthritis.

Chondrocyte is involved in the destruction of joints in osteoarthritis (OA) patients. The aim of this study was to explore the expression level of small nucleolar RNA host gene 5 (SNHG5) and evaluate its function in chondrocyte. In our current study, the expression levels of SNHG5, miR-26a, and SOX2 in 17 pairs of articular cartilage tissues and in the non-OA group were assessed by real-time quantitative reverse-transcription polymerase chain reaction. Results showed that the levels of SNHG5 and SOX2 were significantly downregulated in OA tissues, while the level of miR-26a was upregulated. MTT, colony formation and cell transwell assays were performed to assess the function of SNHG5 on the cell viability, growth ability, and migration capacity in CHON-001 cells. It was found that SNHG5 could promote chondrocyte cell proliferation and migration. The relationship between SNHG5 and miR-26a was confirmed by RIP and the luciferase reporter assays. SOX2 was identified as a target gene of miR-26a by the luciferase reporter assay. Rescue assay was applied to verify the relationship among SNHG5, miR-26a, and SOX2. Our current study demonstrated that SNHG5 is involved in the mechanism of OA through functioning as a ceRNA to competitively sponge miR-26a, therefore, regulating the expression of SOX2.

Dysregulation of both miR-140-3p and miR-140-5p in synovial fluid correlate with osteoarthritis severity.

ObjectivesThis study looked to analyse the expression levels of microRNA-140-3p and microRNA-140-5p in synovial fluid, and their correlations to the severity of disease regarding knee osteoarthritis (OA).MethodsKnee joint synovial fluid samples were collected from 45 patients with OA of the knee (15 mild, 15 moderate and 15 severe), ten healthy volunteers, ten patients with gouty arthritis, and ten with rheumatoid arthritis. The Kellgren–Lawrence grading (KLG) was used to assess the radiological severity of knee OA, and the patients were stratified into mild (KLG < 2), moderate (KLG = 2), and severe (KLG > 2). The expression of miR-140-3p and miR-140-5p of individual samples was measured by SYBR Green quantitative polymerase chain reaction (PCR) analysis. The expression of miR-140-3p and miR-140-5p was normalised to U6 internal control using the 2-△△CT method. All data were processed using SPSS software.ResultsExpression of both miR-140-3p and miR-140-5p was downregulated in OA synovial fluid, showing a statistical difference between the OA and non-OA group, and increased OA severity was associated with a decreased expression of miR-140-3p or miR-140-5p. The Spearman rank correlation analysis suggested that the expression of miR-140-3p or miR-140-5p was negatively correlated with OA severity. In addition, the expression of miR-140-5p was 7.4 times higher than that of miR-140-3p across all groups.ConclusionThe dysregulation of miR-140-3p and miR-140-5p in synovial fluid and their correlations with the disease severity of OA may provide an important experimental basis for OA classification, and the miR-140-3p/miR-140-5p are of great potential as biomarkers in the diagnosis and clinical management of patients with OA.Cite this article: C-M. Yin, W-C-W. Suen, S. Lin, X-M. Wu, G. Li, X-H. Pan. Dysregulation of both miR-140-3p and miR-140-5p in synovial fluid correlate with osteoarthritis severity. Bone Joint Res 2017;6:612–618. DOI: 10.1302/2046-3758.611.BJR-2017-0090.R1.

Comorbidities and health-related quality of life in Koreans with knee osteoarthritis: Data from the Korean National Health and Nutrition Examination Survey (KNHANES).

ObjectivesThis study aimed to evaluate the association of knee osteoarthritis (OA) with comorbidities and health-related quality of life (HRQOL).MethodsA total of 8,907 (weighted n = 13,687,058) participants aged ≥50 years who had undergone knee radiography were selected from the 2010–2012 Korea National Health and Nutrition Examination Survey. OA was classified into four subgroups based on the presence or absence of pain and radiographic OA (ROA): non-OA (Pain-/ROA-), pain only (Pain+/ROA-), ROA only (Pain-/ROA+), and painful ROA (Pain+/ROA+). ROA was defined as Kellgren–Lawrence grade ≥ 2. HRQOL measurements including EuroQOL visual analogue scale (EQ-VAS) scores and the five dimensions and summary index of the EuroQOL-5 dimension (EQ-5D index) were also analyzed. Multivariable logistic regression and linear regression analyses were performed.ResultsAfter adjustment for socioeconomic and lifestyle characteristics, cardiovascular disease, malignancy, and other comorbidities were not significantly associated with OA. Pain only and painful ROA were each significantly associated with limitations in physical activity (odds ratio (OR) 2.66, 95% CI 2.07–3.44, p < 0.001 and OR 2.83, 95% CI 2.25–3.58, p < 0.001, respectively), lower EQ-VAS (β-coefficient = -10.95, p < 0.001 and β-coefficient = -9.75, p < 0.001, respectively), and EQ-5D index (β-coefficient = -0.10, p < 0.001 and β-coefficient = -0.13, p < 0.001) compared with the non-OA group, whereas ROA only was not associated with limitations in physical activity or lower HRQOL score.ConclusionsComorbidities were not significantly associated with knee OA after adjustment. Knee OA was associated with physical activity and HRQOL. Painful knee OA, with or without ROA, was more strongly associated with decreased physical activity and lower quality of life than ROA without pain.

Open Abdomen Improves Survival in Patients With Peritonitis Secondary to Acute Superior Mesenteric Artery Occlusion.

Damage control surgery and open abdomen (OA) have been extensively used in the severe traumatic patients. However, there was little information when extended to a nontrauma setting. The purpose of this study was to evaluate whether the liberal use of OA as a damage control surgery adjunct improved the clinical outcome in acute superior mesenteric artery occlusion patients. A single-center, retrospective cohort review was performed in a national tertiary surgical referral center. Forty-four patients received OA (OA group) and 65 patients had a primary fascial closure (non-OA group) after diagnosed as peritonitis secondary to acute superior mesenteric artery occlusion from January, 2005 to June, 2016. Revascularization was achieved through endovascular aspiration embolectomy, open embolectomy, or percutaneous stent. No difference of bowel resection length was found between groups in the first emergency surgery. However, more non-OA patients (35.4%) required a second-look enterectomy to remove the residual bowel ischemia than OA patients (13.6%, P<0.05). OA was closed within a median of 7 days (4 to 15 d). There was a mean of 134 cm residual alive bowel in OA, whereas 96 cm in non-OA. More non-OA patients suffered from intra-abdominal sepsis (23.1% vs. 6.8%, P<0.01), intra-abdominal hypertension (31% vs. 0, P<0.01), and acute renal failure (53.8% vs. 31.8%, P<0.05) than OA group after surgery. Short-bowel syndrome occurred infrequently in OA than non-OA patients (9.1% vs. 36.9%, P<0.01). OA significantly decreased the 30-day (27.3% vs. 52.3%, P<0.01) and 1-year mortality rate (31.8 % vs. 61.5%, P<0.01) compared with non-OA group. Liberal use of OA, as a damage control adjunct avoided the development of intra-abdominal hypertension, reduced sepsis-related complication, and improved the clinical outcomes in peritonitis secondary to acute SMA occlusion.

Association between osteoarthritis and mental health in a Korean population: a nationwide study.

Osteoarthritis (OA) is a chronic disease and has become a major health problem in recent years. OA causes chronic pain and disability and can induce mental disorders such as stress perception, depression and suicidal ideation. We investigated the incidence of perceived stress, depressed mood and suicidal ideation in OA patients, using the 2010-2012 Korea National Health and Nutrition Examination Survey (KNHANES). A total of 8271 participants were included (3580 male and 4691 female). OA patients were defined as participants with knee/hip joint pain and radiographic changes of the knee/hip joints. Mental disorders included stress perception, depression and suicidal ideation. Odds ratios (ORs) and 95% confidence intervals (95% CIs) for mental disorders were calculated according to the OA. In the multiple logistic regression model, compared to the non-OA group, the ORs of high stress perception, depression and suicidal ideation were 1.59 (95% CI 1.10-2.31), 1.52 (95% CI 1.01-2.29) and 1.90 (95% CI 1.33-2.72), respectively, in male, and 1.41 (95% CI 1.19-1.68), 1.27 (95% CI 1.04-1.54) and 1.49 (95% CI 1.25-1.78), respectively, in female. OA is associated with impaired mental health. OA patients had high stress perception, depression and suicidal ideation. More numbers of male with OA had much more mental disorders than female with OA.

Osteoarthritic Extracellular RNA Biomarkers in Synovial Fluid

The molecular mechanisms influencing the initiation and progression of osteoarthritis (OA) are unclear. Therefore, current clinical management of predominately involves symptomatic treatment of end-stage diagnosed OA. Understanding the pathogenesis of OA at the pre-clinical stage may aid in both diagnostic and preventative modalities in the management of this chronic disease. PURPOSE: To determine the synovial extracellular RNA (exRNA) changes associated with OA. METHODS: Synovial fluid was collected from the injured knee of a cohort of 14 individuals (ages 15-47, 10 males and 4 females) undergoing surgical repair following ACL and/or meniscus injuries. Each knee had arthroscopically graded OA and divided the cohort into 5 individuals in the OA group (OA in >1 knee compartment) and 9 individuals in the non-OA group (OA in ≤1 knee compartment). Total exRNA was extracted from the synovial fluid collected at the time of surgery, >30 million reads generated per sample using massively parallel sequencing, and differential abundance of RNA was calculated between the two groups. RESULTS: A total of 19 protein coding exRNAs were significantly different (FDR ≤ 0.05) between the two groups: 13 increased in the OA group, 6 decreased. While no specific pathways were enriched, these genes included several genes known to influence OA pathways including ADAM12 (metalloprotease-disintegrin 12) and BMPR1A (bone morphogenetic protein receptor type 1A). In addition, 3 miRNAs were different: 2 increased, 1 decreased. One of these increased miRNAs was mir30a which has a known role in promotion of extracellular matrix degradation. CONCLUSIONS: These data suggest that the profile of extracellular RNA molecules are dysregulated arthroscopically diagnosed OA. While the specific intercellular signaling role of these exRNA are yet to be elucidated, they offer intriguing biomarkers and suggestions of dysregulated molecular pathways in OA. Changes in the genes ADAM12, BMPR1A, and mir30a suggest that exRNA markers of extracellular matrix degradation are biomarkers for the initiation and/or progression of OA. These findings have the potential clinical utility to differentiate patients at risk for the development of OA, introducing the possibility for intervention at pre-symptomatic stages.

Symptoms and signs of temporomandibular disorders in patients with knee osteoarthritis

Both temporomandibular disorder (TMD) and knee osteoarthritis (KOA) are prevalent joint diseases; however, an association between them has not been reported. Therefore, this study investigated the prevalence of the specific symptoms and signs of TMDs (SSTs) in patients with KOA. In total, 200 patients with KOA and 150 healthy individuals were recruited. The prevalence of specific SSTs in patients with mild or severe KOA was compared with the prevalence of specific SSTs in the control group and the results were analysed using a chi-square test. Logistic regression was used to adjust for potential confounders, such as gender and age. The prevalence of 'impaired range of jaw movement (IRM)' was 63.6% (n = 77) in the mild KOA group and 62.4% (n = 117) in the severe KOA group; the values for both KOA groups were significantly higher than that for the non-OA control group (34.7%, n = 144; P < 0.017). In addition, 54.7% of the patients with severe KOA reported 'impaired temporomandibular joint (TMJ) function', a value significantly higher than that of the control group (39.6%, P < 0.017). No significant differences between groups were found for other SSTs. Patients with KOA might be more likely to experience SSTs, such as IRM and impaired TMJ function.

Epidemiological characteristics of patellofemoral osteoarthritis in elderly Koreans and its symptomatic contribution in knee osteoarthritis

BackgroundMany studies have reported the prevalence of knee osteoarthritis (OA) but have invariably focused on the tibiofemoral (TF) joint and overlooked the patellofemoral (PF) joint. Accordingly, little epidemiological information is available regarding the PF OA. The purpose of the current study was to document the epidemiological characteristics of PF OA in elderly Koreans. MethodsRadiographic assessment was performed for 681 elderly (≥65years old) Koreans recruited from a community, and symptom severity was evaluated using Western Ontario and McMaster Universities Index (WOMAC) and Short Form-36 (SF-36) scales. Prevalence of different categories of knee OA (isolated PF OA, isolated TF OA and combined PF and TF OA) was calculated. The symptoms of isolated PF OA group and non-OA group were compared. ResultsThe overall prevalence of OA was 22.0% in the PF compartment and 34.1% in the TF compartment. The prevalence of isolated PF OA, isolated TF OA, and combined PF and TF OA was 3.8%, 17.8%, and 19.2%, respectively. Female sex, aging, and obesity were not associated with isolated PF OA. No significant differences were found in any clinical outcome scales between the isolated PF and non-OA groups. ConclusionThis study documents that OA in the PF joint is common in elderly Koreans, but isolated PF OA is rare. Demographic risk factors are not associated with isolated PF OA, suggesting that isolated PF OA may have a different pathophysiology from other types of knee OA. Our study also indicates that the presence of isolated PF OA should not be construed to be responsible for clinical symptoms.

Contribution of the institutional repositories of the Chinese Academy of Sciences to the webometric indicators of their home institutions

Starting from the perspective of Webometrics, this paper explores the improvement effect of institutional repositories (IRs) on their home institutions with respect to web presence and visibility. Taking 19 IRs from institutions affiliated to the Chinese Academy of Sciences (CAS) as study samples, we calculate the contribution of IRs to the webometric indicators of their home institutions in terms of four indicators: page counts, PDF counts, URL mention counts, and link counts. According to their open-access (OA) status, the IRs of CAS were divided into an OA group and a non-OA group, which were compared with respect to differences in the above indicators as well as browse counts and download counts. The results of the study show that: (1) IRs showed a relatively significant positive improvement with respect to Google page counts, Scholar page counts, and Google PDF counts, although the improvement effect with respect to Scholar PDF counts was almost nonexistent; (2) repositories presented a certain improvement effect with respect to URL mention counts, but the contribution of link counts was limited; and (3) OA repositories manifested noticeable advantages in terms of Google PDF counts, URL mention counts, and download counts. We conclude that IRs can improve the web presence and visibility of their home institutions, while OA IRs offer more benefits to their home institutions.

Comparison of the Chemical Composition of Subchondral Trabecular Bone of Medial Femoral Condyle between with Advanced Osteoarthritis and without Osteoarthritis

BackgroundThe purpose of this study is to investigate differences of chemical composition between subchondral bone in advanced osteoarthritic (OA) and non-OA distal femur.MethodsTwenty femurs were harvested, respectively. The subchondral trabeculae were obtained from the middle of medial articular surface of distal femurs. A 10 mm diameter cylindrical saw was used to harvest. Raman spectroscopy, a non-destructive technique, was employed to determine the chemical information of the trabecular bones in the human distal femurs.ResultsThe maximum intensity of the phosphate peak was 2,376.51±954.6 for the non-OA group and 1,936.3±831.75 for the OA group. The maximum intensity of the phosphate peak observed between the two groups was significantly different (P=0.017). The maximum intensity of the amide I peak were 474.17±253.42 for the nonOA group and 261.91±205.61 for the OA group. The maximum intensity of the amide I peak were significantly different between the two groups (P=0.042). Also, among other chemical and matrix components (Hydroxyproline,Carbonate, Amide IIIdisordered;ordered, and CH2), the spectrums showed similar significant differences in the intensity (P=0.027, P=0.014, P=0.012; P=0.038, P=0.029). Area integration were performed to determine disorder in collagen's secondary structure via amide III (alpha helix/random coil). The value of the alpha helix to random coil band area are significantly different (P=0.021) and result showing that there was a trend toward higher collagen maturity for the nonosteoarthritic bone specimens.ConclusionsThe result suggested that OA may affect the chemical compositions of trabecular bone, and such distinctive chemical information may be.

Structural associations of symptomatic knee osteoarthritis.

ObjectiveStructural changes of osteoarthritis (OA) may occur in the absence of pain. In this study, we aimed to identify histopathologic features that are associated with symptomatic knee OA.MethodsMedial tibial plateaus and synovium samples were obtained at the time of total knee replacement (TKR) surgery for OA (advanced OA group) or were obtained postmortem from subjects who had not sought medical attention for knee pain during the last year of life (non-OA control group). To identify features of OA, we compared the patients with advanced OA with the age-matched non-OA controls (n = 26 per group). To identify OA features associated with symptoms, we compared two additional groups of subjects who were matched for severity of chondropathy (n = 29 per group): patients undergoing TKR for symptomatic OA (symptomatic chondropathy group) and postmortem subjects with similar severity of chondropathy who were asymptomatic during the last year of life (asymptomatic chondropathy group). The histologic features of the samples were graded, and immunoreactivities for macrophages (CD68) and nerve growth factor (NGF) in the synovium were quantified. The cellular localization of synovial NGF was determined by double immunofluorescence analysis.ResultsAdvanced OA cases displayed more severe changes in the synovium (synovitis, increased synovial NGF, and CD68-immunoreactive macrophages) and cartilage (loss of cartilage surface integrity, loss of proteoglycan, tidemark breaching, and alterations in chondrocyte morphology) than did the non-OA controls. Synovial NGF was localized predominantly to fibroblasts and to some macrophages. The symptomatic chondropathy group displayed greater levels of synovitis, synovial NGF, and loss of cartilage integrity, in addition to alterations in chondrocyte morphology, than did the asymptomatic chondropathy group (P < 0.05 for each comparison).ConclusionSynovitis, increased synovial NGF, alterations in chondrocyte morphology, and loss of cartilage integrity are features of knee OA that may be associated with symptoms.

Quadriceps muscle strength and its relationship to radiographic knee osteoarthritis

Quadriceps muscle strength and its relationship to radiographic knee osteoarthritis

Morphological evaluation and diagnosis of medial type osteoarthritis of the knee using ultrasound

ObjectiveThe aim of this study was to diagnose osteoarthritis (OA) of the knee joint using ultrasound (US). MethodsThe study subjects included 27 males and 54 females (131 knees), who had a mean age of 62.8 years. US was used to evaluate the medial joint space of both knees in complete extension with and without weightbearing. In each patient, the medial radial displacement of the medial meniscus (MRD), the peripheral joint space (PJS) and the number of osteophytes were evaluated. The subjects were divided into five groups based on the KL grade (0-4), and then each value was compared. Additionally, the subjects were divided into two groups: KL grade 0 and 1 (non-OA group) and KL grade 2-4 (OA group). We classified patients who met the following criteria as having OA on the US assessment: <5 mm of PJS during weight-bearing, >5 mm of MRD during weight-bearing, and >2 mm of osteophytes. Then we examined the diagnostic accuracy of the radiographic diagnosis (non-OA or OA of the knee) using US assessment. ResultsThe PJS was significantly decreased and the MRD was significantly increased in the OA group compared to the non-OA group (p < 0.001). The percentage of radiographic OA of the knee that was correctly diagnosed by ultrasound was 90.8 % (sensitivity), with a specificity of 95.5 %. The positive predictive value was 97.5 % and the negative predictive value was 84.0 %. ConclusionsOur study therefore showed that US has both a high reliability and excellent diagnostic accuracy.

Quadriceps muscle strength and its relationship to radiographic knee osteoarthritis in Japanese elderly

BackgroundKnee osteoarthritis (OA) is a multifactorial disease and strongly affected by mechanical factors. The aims of the present study were to assess validity and reliability of a new muscle strength measuring device, the Quadriceps Training Machine (QTM) and evaluate the relationship between quadriceps strength measured by QTM and radiographic knee OA by epidemiological survey. MethodsThe isometric knee extension muscle strength of QTM was compared with BIODEX in 24 healthy adults. Then, the relationship between radiographic knee OA and quadriceps strength using QTM was investigated with 2,032 knees in 1,016 subjects by an epidemiological survey (Matsudai Knee Osteoarthritis Survey). ResultsSignificant correlation was observed between QTM and BIODEX (r=0.69, 0.82). In the Matsudai Knee Osteoarthritis Survey, the prevalence of radiographic OA (grade II or higher upon Kellgren–Lawrence classification) was: 13, 36.9, 67.8, and 86.5%, regarding women in their fifties, sixties, seventies, and eighties, respectively, and was 1.7, 13.4, 33.5, and 66.2% regarding men, respectively. Quadriceps muscle strength declined following 50years of age, and significant decline was observed in the their sixties and seventies. Quadriceps muscle strength of the OA group (grades II, III and IV) was significantly declined compared with that of the Non-OA group (grade-0 and I). Furthermore, the tendency of the muscle strength level to decline with the progression of knee OA grade was particularly observed between grade 0 and grade I in both men and women and between grade I and grade II in men. ConclusionThe relationship between radiographic knee OA and quadriceps strength was quantitatively evaluated by an epidemiological survey, and we found a correlation between knee OA and the decline in quadriceps strength. Furthermore, it was suggested that the decline in quadriceps muscle strength may be more strongly related to the incidence of knee OA than to its progression.

Quadriceps muscle strength and its relationship to radiographic knee osteoarthritis

Quadriceps muscle strength and its relationship to radiographic knee osteoarthritis

The excess burden of osteoarthritis in the province of Ontario, Canada

Little is known about the burden of osteoarthritis (OA) in Canada. This study was undertaken to estimate the excess burden of OA in Ontario, the largest province in Canada. The records of Ontarian respondents to the Canadian Community Health Survey (CCHS) who provided consent to data linkage were linked to the Ontario Health Insurance Program physician claims database and the Discharge Abstract Database Inpatient and Day Procedure databases. Patients with OA (n = 1,474) were identified using CCHS 1.1 and the physician claims database. To determine the excess burden of OA, a control group matched by age, sex, and rural/urban status was created, with 3 controls per case (n = 4,422). Sociodemographic and medical characteristics, health-related quality of life, and 1-year physician, day (outpatient) procedure, and hospitalization costs were compared between the 2 groups. Regression analyses were performed to identify predictors of medical characteristics, health utility, and cost. The mean age of the OA patients and the control subjects was 66 years, and 74% of all study subjects were women. Several differences were observed between patients with OA and subjects without OA in terms of socioeconomic and medical characteristics. On a scale of 0-1, the mean utility value associated with OA was 0.68, compared to 0.84 for the control group (P < 0.0001), representing a utility decrement of 0.16. The 1-year physician, outpatient procedure, and hospitalization costs were significantly higher in the OA group than in the non-OA group ($2,233 Canadian versus $1,033 Canadian, respectively; P < 0.0001). These results indicate that the excess burden of OA in Ontario is considerable.

Anteroposterior and varus–valgus laxity of the knee increase after stair climbing in patients with mild osteoarthritis

The aim of this study was to measure exercise-induced changes in knee joint laxity in patients with knee osteoarthritis (OA). The study subjects were 46 female patients with OA and 22 age- and sex-matched normal controls. Radiographs of the knee were taken in all subjects, and the disease severity was graded according to the Kellgren and Lawrence (K-L) grading system. The K-L grade of the control subjects (non-OA group) was 0-1. The OA patients were divided into those with mild OA (K-L grade 2, n = 20) and advanced OA (K-L grade 3-4, n = 26). The subject climbed up and down 8 steps on a staircase apparatus over a period of 10 min. The anteroposterior (A-P) translation was measured with KT2000 arthrometer, and varus-valgus (V-V) rotation was measured on stress radiographs before and after the stair climbing. The Δchange in A-P translation after the exercise was significantly larger in mild OA group than other groups (P < 0.005). The Δchange in V-V rotation after exercise was significantly larger in mild and advanced OA groups than the control (P < 0.003). There were no significant differences in A-P laxity and V-V laxity before exercise among the non-OA, mild OA and advanced OA groups. Exercise resulted in significant changes in A-P knee joint laxity in patients with mild OA relative to the control. The results suggest that daily physical activities (e.g., knee bending or squatting) play a role in the development of knee laxity, particularly in patients with mild OA, and that progression of knee OA seems to correlate with increments of A-P knee joint laxity.

Prior Oral Antithrombotic Therapy is Associated with Early Death in Patients with Supratentorial Intracerebral Hemorrhage

Although oral antithrombotic therapy (OAT) is a risk factor of intracerebral hemorrhage (ICH), the clinical course of supratentorial ICH with prior OAT is unclear. We therefore assessed the characteristics of supratentorial ICH with OAT to determine whether OAT is independently associated with early death in supratentorial ICH. We retrospectively enrolled consecutive patients with supratentorial ICH admitted to the Stroke Center of Kawasaki Medical School Hospital within 24 hours of onset, from April 2004 to March 2009. The group with OAT therapy (OA group) was compared with the group without (non-OA group). A total of 389 patients with supratentorial ICH (median age 68 years, 61% males) were enrolled in the present study. OAT was used in 24% of patients. The OA group was older than the non-OA group (median 74 vs. 66 years, p<0.001). In the OA group, Glasgow Coma Scale was less (10 vs. 13, p<0.001), and hematomas were larger (22 mL vs. 14 mL, p<0.001). Early death was more frequently observed in the OA group than in the non-OA group (28% vs. 8.1%, p<0.001). Unadjusted HR of OAT for death within 14 days was 3.62 (95% CI: 2.06-6.33, p<0.001), the age- and sex-adjusted HR was 3.84 (95% CI: 2.12-6.96, p<0.001), and HR adjusted for age, sex, GCS, and hematoma volume was 2.01 (95% CI: 1.11-3.65, p=0.022). HR adjusted for age, sex, GCS, and hematoma volume at day 1 was 2.63 (p=0.34), day 3: 2.35 (p=0.03), day 7: 2.01 (p=0.04), and day 14: 1.90 (p=0.04). The OA group patients were older, their GCS was lower, they had larger hematoma volume, and more frequent occurrence of early death. Prior oral antithrombotic therapy is associated with early death in patients with supratentorial ICH.

노인 여성 관절염 환자의 보행시간에 따른 지면반력의 특징

The purpose of this study was to analyze and compare the effect of gait time on the gait patterns in elderly female OA and non-OA patients. The intensity of the subjects joint pain was surveyed by using WOMAC. Twelve subjects participated in this study. Measurements were taken for every the 10 minutes for 30 minutes after walking by a force plate. The following variables were recorded; double stance support time, Fx, Fy, Fx_time, Fz and so on. From the investigation of these variables the following was observed and concluded. The Fz values for the OA group was 1.01BW at the start and after 30 minutes was 1.04BW on the other hand the non-OA group's Fz1 was 1.08BW and 1.10BW. There was no significant difference calculated between the two groups and at the start and end of the experiment about all the variables. Therefore, it is concluded that there was no negative effect caused by walking for the 30 minutes and that it was an effective way of strengthen both respiratory and muscle function.

Accumulation of mitochondrial DNA with 4977-bp deletion in knee cartilage – an association with idiopathic osteoarthritis

Since mitochondrial DNA (mtDNA) mutations have been established to associate with the aging process and some degenerative diseases, we investigated the correlation between idiopathic osteoarthritis (OA) and the 4977-bp mtDNA deletion. Cartilage were collected from six sites in knee joints removed from 18 aged patients with idiopathic OA, 10 aged non-OA cadavers, 3 young cadavers (YC), and lateral femoral condyle of 9 young patients. Histopathologic changes were examined and the common 4977-bp mtDNA deletions were analyzed in young and elderly cartilages obtained from different sites in the knee joint. The association of the 4977-bp deletion of mtDNA with idiopathic OA and aging was evaluated. The 4977-bp mtDNA deletion was detected in 17 of the 18 OA patients, 9 of the 10 aged non-OA cadavers, and 1 of the 3 YC. None of the nine specimens collected from the lateral femoral condyle of young patients had a detectable deletion of mtDNA. The 4977-bp mtDNA deletion was not significantly correlated with the severity of OA graded by the Mankin score. The frequencies of occurrence of the 4977-bp mtDNA deletion were significantly different between the OA group and the aged non-OA control group (P=0.004) and between the aged non-OA group and the young control group (P=0.002). The results suggest that accumulation of the 4977-bp deletion of mtDNA in knee cartilage increases with age and may play a role in the development of idiopathic OA in the knee joint.