Non-labile Fraction Research Articles

Effects of additives on shifting phosphorus to solid phase during Solid-Liquid separation of digestate in full-scale biogas plant

Phosphorus (P) is critical for plant growth, but global reserves are exhausting within 250–300 years, therefore enhancing phosphate recycling is crucial for the future. Biogas digestate, rich with nutrients is a promising resource for nutrient recovery. Conventional solid–liquid separation shifts approximately 35 % of the total P in the digestate to the solid phase. Separation trials with additive treatment using a screw press with a 0.75 mm sieve were performed at University of Hohenheim’s full-scale biogas plant. After 22 h, 67.41 % (kieserite treatment) and 52.35 % (strawflour treatment) of total P shifted to the solid phase. Treatment with kieserite enhanced P shift into the solid phase by forming non-labile fractions through a chemical bond between P and Mg2+ ions. Kieserite treatment for 22 h effectively increases the share of total P in the separated solid phase, it also ensures a sustainable nutrient supply and mitigates the risk of nutrient runoff.

Phosphorous Fractions in Soils of Natural Shrub-Grass Communities and Leucaena leucocephala Plantations in a Dry-Hot Valley

Afforestation is an effective approach for restoring degraded ecological functions in the dry-hot valleys of southwest China. Afforestation can affect soil carbon and nitrogen storage; however, how it affects soil P fractions, and their driving factors. is poorly understood in this region. To address these questions, we conducted a field study of Leucaena leucocephala plantations at three different stand age sites (3, 10, and 20 years) and an adjacent natural shrub-grass community control site to investigate changes in soil total phosphorus (Pt), Pi (inorganic phosphorus), Po (organic phosphorus), and phosphorus (P) fractions and their driving factors. Soil Pt, Po, labile P, and moderately labile P significantly increased in the Leucaena leucocephala plantation compared with the natural shrub grass site, and the Leucaena leucocephala plantation increased soil Pt content by significantly increasing soil Po. Soil Pt, Po, Pi, labile P, moderately labile P and non-labile P were not significantly different among the different stages of the Leucaena leucocephala plantation, and soil Pt and its fractions were all significantly higher in the middle-age forest stage of the Leucaena leucocephala plantation. These results indicate that Leucaena leucocephala plantations increased the soil P transformation ability, and soil Po played a critical role in sustaining soil P availability. The middle-age forest stage of Leucaena leucocephala plantations had the best conditions for P stocks and P conversion capacity. The abundance of actinomycetes and fungi showed significant positive relationships with soil Pi fractions (NaHCO3-Pi, NaOH-Pi, and NaOHu.s.-Pi); soil Pt and moderately labile P were significantly and directly influenced by fungal abundance. Soil organic carbon (SOC), NH4+-N, and NO3−-N showed significant and positive relationships with the soil Pi fractions (NaHCO3-Pi, NaHCO3-Po, and HCl-Po). SOC and NO3−-N were the key drivers of soil Pt, labile P, moderately labile P and non-labile fractions. These results indicate that abiotic and biotic factors differently affected the soil P fractions and Pt in Leucaena leucocephala plantations in the dry-hot valley.

Fractions of Organic Matter and Soil Carbon Balance in Different Phases of an Agroforestry System in the Cerrado: A Ten-Year Field Assessment

Integrated production systems composed of trees, crops and pastures have shown good results in improving soil quality and the capacity to store carbon in the soil, being efficient in mitigating greenhouse gas emissions. Despite this, changes in carbon stocks and soil organic matter fractions in the initial stages of implementing an agroforestry system remain unclear. This study evaluated the carbon balance and the dynamics of soil organic matter fractions in an agroforestry system conducted over a decade. Total carbon, labile carbon, carbon from particulate organic matter, organic carbon associated with minerals and inert carbon were determined at depths 0–10 cm, 10–20 cm and 20–40 cm. Soil carbon stocks were also estimated for the 0–40 cm depth. Total carbon increased in the agroforestry system compared with a low-productivity pasture. The total carbon stock in the last growing season (68.57 Mg ha−1) was close to the original soil stocks under native Cerrado vegetation (76.5 Mg ha−1). After 10 years, there was a positive balance in the soil carbon stock of both the total carbon and the soil organic matter fractions. The successional agroforestry system is a good alternative to increasing soil total carbon stocks and labile and non-labile fractions of soil organic matter.

Effect of Reactive and Non-Reactive Additive Treatment on the Recovery of Phosphorus from Biogas Digestate

The annual phosphate (PO43−) utilization has increased, leading to a depletion of existing sources of phosphorus (P). To overcome this, digestate as a source to recover P is being investigated. Due to the abundance of nutrients, the digestate from an agricultural biogas plant is used as fertilizer for crops. The separation of solids and liquids from the digestate by a screw press is the simplest form of concentrating, therefore, recovering PO43−. This is the most commonly employed method in existing biogas plants. However, the separation is not very efficient as only 20–30% of P is recovered in the solid phase. The goal of this study is to increase the separation efficiency and recover more P into the solid phase, in order to improve the transportability. For this, separation trials at a laboratory scale were performed for five experimental groups, with biochar and straw flour as non-reactive additives and kieserite as a reactive additive. In addition, untreated digestate was studied as a control. The control and the treatment with biochar and straw flour were carried out at 25 °C, while the treatment with kieserite was performed at 25 °C and 50 °C. The separation trails were performed at treatment times of 0 h, 1 h, 2 h, 8 h, and 20 h. The results showed that the treatment with additives had a beneficial effect on the recovery of P. It was noted that kieserite treatment at 25 °C and 50 °C bound about 61% of the total P present in the digestate to the solid phase. A sequential extraction was performed to study the effect of additives on the recovery of different P species. The results concluded that, compared to biochar and straw flour, kieserite was efficient in recovering the non-labile fractions (NaOH-P and HCl-P) of P, which act as slow-release fertilizers. This study shows that the use of additives, especially kieserite, has a positive influence on recovering P from digestate, and further research to optimize the recovery process would be beneficial.

Effects of different carbon substrates on PAHs fractions and microbial community changes in PAHs-contaminated soils

Different types of carbon substrates were widely used in soil remediation. However, differences of their impacts and related mechanisms on degradation of polycyclic aromatic hydrocarbons (PAHs) and microbial community structures in contaminated soil still remain unclear. Here, we investigated the effects of corn straw (S), glucose (G), straw combined with glucose (SG), and sodium azide (N, as an abiotic control) on PAHs fractions and bacterial communities in soil. After 70 days’ microcosm experiments, total PAHs concentrations were significantly reduced by 30.9%, 19.5% and 44.6% under S, G and SG treatments. Water soluble, acid soluble and residual PAHs under all treatments were significantly decreased after 70 days of incubation, while organically bound PAHs were increased by 11.4%, 22.7% and 36.1% under G, S and SG treatments. Additionally, straw and glucose application increased relative abundance related PAHs-degrading bacteria and the copy numbers of gram-negative (PAHs-RHDα GN) and gram-positive genes (PAHs-RHDα GP) in the contaminated soil. Redundancy analysis (RDA) and Random Forest (RF) indicated that PAHs fractions are crucial factors for biodegradation of PAHs in PAHs-contaminated soils amended with carbon substrates. These suggested that carbon substrates contributed to PAHs conversion from residual PAHs (nonlabile fractions) to organically bound PAHs and thus increased the potential for PAHs conversion to water-soluble and organic acid-soluble PAHs, which were more easy to be utilized by soil microorganisms. This study revealed the new insights of different carbon substrates on degradation and dynamic changes of PAHs fractions and the better potential of combined application of straw and glucose in enhancing degradation of PAHs in PAHs-contaminated soils.

Soil organic carbon fractions under different land use systems of Jorhat district

The present study was conducted using soil profiles of Jorhat district under different land use systems (LUS) i.e. Bamboo field, tea plantation and rainfed rice. The total soil organic carbon content at 0-15 cm depth varied from 7.86 to 8.43 g kg-1, wherein rainfed rice land use system recorded significantly higher total organic carbon (TOC) than tea plantation but at par with that of bamboo field. However, at 15-30, 30-60 and 60-100 cm soil depths bamboo field recorded significantly higher over other land use systems. Among these LUS, rainfed rice showed significantly higher very labile carbon (CVL) in soil than tea plantation and bamboo field at 0-15 cm depth whereas in 15-30 cm depth CVL in tea plantation was found to be significantly higher than bamboo field and rainfed rice. However, Bamboo field and tea plantation had CVL significantly higher than rainfed rice under 30-60 and 60-100 cm depths, respectively. In all the depths, labile carbon pool (CL) was significantly higher in bamboo field ranging from 1.01 to 1.67 g kg-1 whereas under bamboo field, less labile carbon (CLL) pool 0-15, 15-30 and 30-60 cm depths was significantly higher than tea plantation and rainfed rice soils. However, the recalcitrant carbon (CR) pool did not vary significantly among different LUS. Soil organic carbon stock in soil at 0-15 cm depth was found to be significantly higher in rainfed rice soil than tea plantation and bamboo field whereas in the remaining depths the SOC stock was significantly higher under bamboo field. Moreover, the interaction between LUS and depths varied significantly. Among different fractions (CL, CLL, CVL and CR fractions), CR fraction had the smallest variation among land uses, which indicate that the non-labile fraction was less sensitive to land use systems.

Distribution of soil carbon fractions under different bamboo species in northwest Himalayan foothills, India.

Soil carbon and its fractions are important in understanding the mechanism of soil carbon sequestration. The present study evaluated the impact of seven commercial bamboo species, viz., Bambusa balcooa, B. bambos, B. vulgaris, B. nutans, Dendrocalamus hamiltonii, D. stocksii, and D. strictus, on labile and non-labile carbon fractions. In the 0-15-cm layer, B. nutans had the highest very labile C (7.65gkg-1) followed by B. vulgaris > B. balcooa > D. stocksii > D. hamiltonii > B. bambos > D. strictus > open. The active carbon pool was significantly low under the control plot (i.e. the open) indicating the positive influence of bamboo in soil C build-up in the top 0-15cm soil layer. Amongst the different species of bamboo evaluated in this study, D. strictus accumulated the highest active C pool in 0-30-cm soil layer followed by B. vulgaris. Of the total organic C in the 0-30cm soil depth, majority (55-60%) was contributed by the passive C pool comprising the less labile and the non-labile fraction of SOC. A high value of carbon stratification ratio (> 2) was observed for D. strictus, B. bambos, and D. hamiltonii which proves their potential for restoration of the degraded lands. The majority of bamboo species except for B. balcooa and D. stocksii showed a higher carbon management index than open systems, thereby indicating higher rates of soil C rehabilitation. Of the seven bamboo species, B. vulgaris, D. strictus, and B. nutans can be adopted for cultivation in the northwest Himalayas given their ability to positively impact the SOC and its fractions in both surface and sub-surface soil.

Desorption Isotherms for Poly- and Perfluoroalkyl Substances in Soil Collected from an Aqueous Film-Forming Foam Source Area

Bench-scale experiments were performed to evaluate poly- and perfluoroalkyl substance (PFAS) desorption from vadose zone soils collected at a site historically impacted with aqueous film-forming foam (AFFF). A sequential dilution batch method was employed to obtain PFAS desorption isotherms from a shallow surface soil [0.03 to 0.9 m below ground surface (bgs)] and from a deep soil (0.9–2.4 m bgs). Results showed that the desorption isotherms were reasonably described by a linear model, and that a fraction of the soil-bound PFAS mass was not readily desorbed (or, non-labile); the recalcitrance of this non-labile fraction was evaluated on a subset of samples and shown to persist for up to 9 months. The non-labile fraction of PFAS mass sorbed to the soil (non-labile PFAS sorbed mass divided by the total PFAS sorbed mass) ranged from 0.17 g g−1 for perfluorohexane sulfonamide in the deep soil to 0.87 g g−1 for 8∶2 fluorotelomer sulfonate in the shallow soil, and generally increased with the PFAS organic carbon:water partitioning coefficient (Koc). The fraction of the PFAS within the non-labile fraction was generally greater in the shallow soil than in the deep soil, likely due to the elevated organic carbon content of the shallow soil. The slopes of the desorption isotherms associated with the readily-desorbable (labile) PFAS fraction for the shallow soil were within the ranges predicted by Koc-based models derived from adsorption data. Overall, results for the soils studied herein suggest that much of the PFAS mass present in the soil likely is not readily available for leaching into infiltrating waters. Such information could provide useful insight for developing PFAS soil cleanup standards.

Organic amendments affect soil organic carbon sequestration and fractions in fields with long-term contrasting nitrogen applications

Organic amendments are widely used to both manage soil organic C (SOC) and improve soil fertility in agro-ecosystems. However, crop production and the mechanisms of SOC sequestration following organic amendment may be affected by long-term differential N fertilizations, hence driving the dynamics in SOC fractions in cultivated fields. Our 3-yr (2016–2019) field experiment evaluated the responses of SOC sequestration and SOC fractions to organic amendments in soils with long-term contrasting N applications in a winter wheat monoculture system. We compared wheat straw return (St) and farmyard manure application (Fm) in soils with either long-term (2002–2019) N absence (-N) or normal N application (+N). Both St and Fm increased SOC stocks in the 0–20-cm layer, but Fm increased it more than St did. However, St exhibited a greater SOC sequestration rate in +N soils than that in –N soils, whereas Fm exhibited both a lower SOC sequestration rate and efficiency in +N soils than in –N soils. SOC increased across all fractions in all soils after both St and Fm. However, compared with –N soils, +N soils exhibited a lower SOC increase, specifically in the very labile fraction after St, but in the non-labile fraction after Fm. St, Fm, and +N all increased the soil C management index (CMI), but only +N increased wheat yield and its sustainable yield index (SYI). Those relationships indicated that both straw and manure amendments were responsible for both SOC sequestration and increasing the CMI, and N input was fundamentally responsible for an increased SYI and associated plant-C input. Consequently, straw return was expected to be more effective at sequestering SOC when applied to soil having had adequate long-term N application, whereas farmyard manure application was likely more effective when applied in N-deficient soil. Our field study findings provide empirical evidence that soil N availability affects SOC sequestration capacities following organic amendments, and that impacts strategies aimed at further increasing soil fertility improvements via organic amendment, especially considering that different agro-ecosystems are subject to different fertilization regimes.

Microbial induced carbonate precipitation contributes to the fates of Cd and Se in Cd-contaminated seleniferous soils

Bioremediation based on microbial induced carbonate precipitation (MICP) was conducted in Cd-contaminated seleniferous soils with objective to investigate effects of MICP on the fates of Cd and Se in soils. Results showed that soil indigenous microorganisms could induce MICP process to stabilize Cd and mobilize Se without inputting exogenous urease-producing strain. After remediation, soluble Cd (SOL-Cd) and exchangeable Cd (EXC-Cd) concentrations were decreased respectively by 59.8% and 9.4%, the labile Cd measured by the diffusive gradients in thin-films technique (DGT) was decreased by 14.2%. The MICP stabilized Cd mainly by increasing soil pH and co-precipitating Cd during the formation of calcium carbonate. Compared with chemical extraction method, DGT technique performs better in reflecting Cd bioavailability in soils remediated with MICP since this technique could eliminate the interference of Ca2+. The increase in pH resulted in Se conversion from nonlabile fraction to soluble and exchangeable fractions, thus improving Se bioavailability. And Se in soil solution could adsorb to or co-precipitate with the insoluble calcium carbonate during MICP, which would partly weaken Se bioavailability. Taken together, MICP had positive effects on the migration of Se. In conclusion, MICP could stabilize Cd and improve Se availability simultaneously in Cd-contaminated seleniferous soils.

Soil Carbon Fractions at Different Soil Depths as Influenced by Land use Practices under Cropping Systems in a Vertisol

The experimental field study was conducted at Borlaug Institute for South Asia (BISA) Research Farm, Lakhanwada, Jabalpur, Madhya Pradesh, India to evaluate the soil carbon fractions (very labile, labile, less labile and non-labile or recalcitrant carbon) in different land use practices with soil depths under cropping systems in Vertisols after harvest of Kharif and Rabi season crops of year 2015-16 and 2016-17. The experiment was conducted under Split plot design considering land use practices as main plot treatments [L1: Uncultivated, L2: rice-wheat system with conventional agriculture (CT), L3: rice-wheat system with conservation agriculture (CA), L4: soybean-wheat system with CT, L5: soybean-wheat system with CA, L6: maize-wheat system with CT and L7: maize-wheat system with CA] and depth (0-5 cm, 5-15 cm and 15-30 cm) as sub-plot treatments replicated thrice. Very labile carbon fraction was obtained highest in L3 (rice-wheat system with CA) and lowest under L6 (maize -wheat system with CT) treatment after harvest of Kharif and Rabi season crops during 2015-16 and 2016-17and it was significantly higher at 0-5 cm soil depth than those in 5-15 cm and 15-30 cm soil depths. Similar trends were also obtained in case of labile, less labile and non-labile fraction of carbon i.e. the applied land use practices had significant effect on all the carbon fractions under study and found to be maximum under L3 (R-W system with CA) and minimum in L6: (M-W system with CT) treatment after harvest of both the season crops during both years of experiment. Whereas, the interaction effect of land use practices and soil depths on the carbon fractions was found statistically non-significant during both the seasons and years.

Environmental and geochemical characterization of alkaline mine wastes from Phalaborwa (Palabora) Complex, South Africa

A detailed characterization of alkaline tailing ponds and waste rock dumps from Phalaborwa Igneous Complex (PIC) South Africa, has been accomplished. The study goes beyond the environmental characterization of mining wastes, offering the first insight towards the recycling of the wastes as alkaline reagent to neutralize acid industrial wastewater. To achieve these aims, tailings and waste rocks were characterized using a combination of conventional, novel and modified Acid Rock Drainage (ARD) prediction methodologies, as well as South African leachate tests, sequential extractions and pseudo-total digestions. The scarcity of Fe-sulphide minerals and the abundance of alkaline minerals indicated that PIC wastes are not ARD producers. The highest neutralization potential was found in the carbonatite rocks and East tailing samples (range between 289 and 801 kg CaCO3 eq/t). According to the National Environmental Management Waste Act (59/2008) of South Africa, tailing ponds and waste rock dumps from PIC classify as non-hazardous (Type 3 waste). The sequential extractions showed that the different fractions from most of the samples would mostly release sulphate and non-toxic elements, such as Ca, Mg, Na and K, which might be a concern if leached in high concentration. In addition, relatively high concentrations of radionuclides, such as U and Th (average of 6.7 and 36.3 mg/kg, respectively) are present in the non-labile fraction of PIC wastes, while the leachable concentrations were always below 0.006 mg/L. Among PIC wastes, East tailing would be the best option as alkaline reagent to neutralize acid wastewater because of its high neutralization potential and non-harmful leachate composition. In general, this study exposes the shortcomings in mine waste characterization, particularly for alkaline mine wastes, and introduces the assessment of potential revalorization as a novel practice in mine waste characterization that, if extended as a regular practice, would facilitate a circular economy approach to the mining industry with its consequent economic and environmental benefits.

Forms of inorganic phosphorus in carbonatic soils in the Pantanal of Mato Grosso do Sul, Brazil

The western region of the southern Pantanal is characterised by soils with high phosphorus (P) contents, derived from materials from the surrounding lithostratigraphic units, accumulated by fluvial transport. However, studies on forms and availability of P in these soils are scarce. The objective of this study was to evaluate the different forms of inorganic P and their relationship with some attributes of carbonatic soils in the Pantanal of Mato Grosso do Sul, Brazil. Hedley’s sequential fractionation scheme was used to evaluate the forms of P in soil profiles: Kastanozems (profiles P1 and P3) and Gleysol (profile P2). Total P contents were similar in the three profiles, 3782–5637 mg kg–1, with mean values of 22% for organic P and 46% for inorganic P (P.i). The P.i results indicated that in the profiles there was a predominance of inorganic forms of P in the following order: P-NaOH 0.5 mol L–1 > P-NaHCO3 > P-NaOH 0.1 mol L–1 > P-HCl, that is, adsorbed to microaggregates, labile, adsorbed to oxides and precipitated with calcium (Ca), respectively. The highest values of total organic P were verified in the surface horizons, with high correlation with total P contents. Residual P contents were high in all profiles, representing 29.0–33.3% of the total P, being correlated with CaCO3 contents. The studied profiles had high contents of labile P, with the highest values in the fraction P.i-NaHCO3, possibly associated with the processes of reduction of iron during the periods of floods, making the adsorbed P available. The contents of P.i-NaOH 0.1 mol L–1, a moderately labile fraction adsorbed to oxides, showed few differences compared to the non-labile fractions associated with Ca (P.i-HCl). Possibly, organic matter was bound to Ca, inhibiting the formation of precipitates of Ca with P and making P available for the more labile fractions.

Long term impact of different tillage systems on carbon pools and stocks, soil bulk density, aggregation and nutrients: A field meta-analysis

Intensive tillage frequently has adverse impact on soil physical quality and soil organic carbon stocks in temperate regions. A consequence of this has been a reduction in crop production over the years demonstrating the necessity of effective and sustainable agriculture. This study aimed to evaluate soil organic carbon (SOC) storage and pools, soil bulk density, aggregation and nutrient availability under different tillage systems (no-tillage, chisel and conventional tillage), based on a long field experiment. After 10 years of crop rotation, in 0–30 cm soil depth, no-tillage system had higher total organic carbon over conventional tillage treatment. Plots under no-till and chisel treatments in 0–10 cm depth, had 7–13%, 34–35% and 9–15% higher non-labile fraction (Cfrac4), very-labile fraction (Cfrac1) and total organic carbon (TOC), respectively compared to that of conventional ones. Also, no-till system had higher passive carbon pool and carbon management index than conventional system. In addition, the conservative tillage, reduced soil bulk density compared with conventional tillage in the surface layer. The stability of slow-wetting and room storage aggregates from conservative tillage were significantly higher (p < 0.05) than conventional tillage. Overall, stability of soil aggregates is improved under conservative tillage. Tillage system, crop rotation and residues, increased soil available nutrients N, P and K in the 0–10 cm depth, with the highest values in no-tillage system. Therefore, conservation tillage could be recommended as a tillage practices that improve fertility soil characteristics and production sustainability.

Fractionation and mobility of copper, cadmium, and lead in some soil irrigated with sewage water in Qena governorate, upper Egypt

This study was conducted to investigate the chemical fractionation and mobility of Cu, Cd and Pb in some soil irrigated with sewage water in Qena governorate, Upper Egypt. Sequential extraction results showed that in the treated soil, a major proportion of Cu, Pb, and Cd was associated with poorly crystalline iron oxide bound (PCFeOX), crystalline iron oxide – occluded (CFeOX) and a major proportion of Cu, Pb and Cd was associated with residual (RES) fractions. Based on relative percent. the results also show that The non-labile Cu, Cd and Pb in the non-irrigated soil partially transformed to the potentially labile Cu due to the irrigation with sewage water Also, a part of the readily labile and potentially labile Cu, Cd and Pb of the sewage water irrigated soils for short time transformed to the non-labile fraction with irrigation with same water for a long time. Higher mobility of metals in the soils irrigated with sewage water for short time represent a potential hazard for the redistribution and translocation of these metals into the food chain and leach to ground water.

Sewage Sludge Application in Eucalyptus urograndis Plantation: Availability of Phosphorus in Soil and Wood Production

Phosphate fertilizers used in planted forests mainly come from scarce and non-renewable sources, and there is a need for new sources of phosphorus (P). An alternative is the use of sewage sludge (SS), rich in organic matter, nitrogen and P. The objectives were to evaluate the soil P availability and its effect on wood production in 22-months old Eucalyptus urograndis plantation treated with SS. A field experiment was set up in a randomized complete block design, four replicates, with the following treatments: control without fertilization; mineral fertilization (MF); 14.5 Mg ha-1 of SS + 22 kg ha-1 of P (S1P1); 29 Mg ha-1 of SS (S2); 29 Mg ha-1 of SS + 17.5 kg ha-1 of P (S2P2); and 43.5 Mg ha-1 of SS (S3), dry base. Of total P in SS, 65% was in organic form, and 42% was in labile + moderately labile forms. S2P2 and S3 positively altered the pattern of soil P distribution, with more P in the labile and moderately labile fractions than non-labile fraction, along 0-20 cm depths, than MF and control. There were higher microbial and available P as a function of SS dose. Wood volume and biomass were highly related to soil P availability as a function of SS dose. Within the SS treatments, S2P2 resulted in higher gains of volume and biomass of wood. The SS application at recommend rate, supplemented with 66% of recommended P fertilizer dose in Eucalyptus plantations, can reduce the use of P fertilizer by 33%, N fertilizer by 100% and maintain comparable soil P availability and wood production.

Dynamic of P Flux in Tropical Acid Soils Fertilized with Humic Acid–Complexed Phosphate

Soils are characterized by high phosphorus (P) immobilization, considering the major limiting factor for agricultural production in Brazil. Phosphate fertilizers complexed with humic acids (HA) have been presented as an alternative to inhibit P immobilization in soils; however, little is known regarding its efficiency or its effect on soil P dynamics when associated with liming. The objective was to investigate labile moderately and non-labile P changes in sandy and clayey soils under application of distinct P sources and liming. Tropical soils were incubated using 200 mg kg−1 of P (simple superphosphate—SSP and SSP + HA), associated or not with calcium oxide. Check-plots were run in both soils without any P addition and/or no liming. After 60, 90, and 180 days of incubation, P contents were fractionated into P inorganic (Pi) and organic (Po), in the following pools: labile (PAER and PBIC), moderately labile (PHCl and PHID-0.1), and non-labile fractions (PHID-0.5 and Presidual). There was a P flux between non-labile and labile fractions, controlled by the use of P fertilizer and liming. In sandy soil, the P addition promoted a clear P flux from non-labile to labile fractions; however, in clayey soil, there was a reverse P flux. The lack of P addition promotes a negative balance in soil with a decrease of labile fractions, mainly when associated with liming. Therefore, inputs are necessary to avoid soil P depletion.

Long‐term fertilization does not affect soil carbon/nitrogen/sulfur ratios or the proportion between labile and nonlabile fractions in Mollisols

AbstractAgricultural management practices such as fertilization may affect the overall soil quality and nutrient supply capacity. In this study, we hypothesized that long‐term fertilization promotes (a) an increase in the proportion of easily degradable soil organic C, N, and S fractions and (b) changes in the soil C, N, and S stoichiometric ratios in the direction of favoring the specific nutrient added to the soil. We included S in our study due to the increasing importance of S fertilization in many agricultural soils. A long‐term experiment involving six fertilization treatments and maize (Zea mays L.), soybean [Glycine max (L.) Merr.], and wheat (Triticum aestivum L.) as cash crops was conducted for 14 yr in an on‐farm network located in the Pampean region (Argentina). Long‐term fertilization did not have a central role in defining the C, N, and S distribution between soil organic fractions or their stoichiometric ratios since the particulate organic matter (POM)‐C/total C, POM‐S/total S, C/N, C/N, and N/S ratios were not affected by fertilization treatments and the mineralizable N/total N ratio varied in a very short range. Instead, long‐term fertilization increased residue C inputs to the soil (8.7–19 Mg ha−1 over the unfertilized control) and, in turn, increases in soil total C, N, and S followed a linear relationship with residue inputs. This relationship was not affected by fertilization or site factors. These data contribute to understanding of the effects of continued fertilization on soil properties such as the distribution of fast‐ and slow‐cycling organic matter and nutrient concentrations, which are essential for effectively managing soils for sustainable agriculture.

Responses of Soil Organic Carbon Fractions to Land Use Types in Hilly Red Soil Regions, China

Land use type exerts important influences on soil organic carbon (SOC) and its fractions, and determines the stability of the carbon pool. Taking woodland as a reference, the content of SOC and its labile fractions[dissolved organic carbon (DOC), microbial biomass carbon (MBC), and particulate organic carbon (POC)] and non-labile fractions[mineral-associated organic carbon (MAOC)] in upland and paddy surface soils in hilly red soil regions were determined to explore the responses of SOC fractions to land use type. The results showed that the contents of SOC, MBC, POC, and MAOC ranked highest in paddy compared with upland and woodland. DOC content in woodland was significantly higher than in upland and paddy (P<0.001). The proportion of each SOC fraction, i.e. DOC/SOC, MBC/SOC, POC/SOC, and MAOC/SOC, was in the range of 0.22%-0.93%, 1.62%-2.70%, 31.08%-40.00%, and 43.22%-56.82%, respectively. The contents of labile fractions (MBC and POC) and their proportions (MBC/SOC and POC/SOC) were in the order of paddy > woodland > upland. MAOC content ranked the highest in paddy but the lowest in upland, while MAOC/SOC exhibited the opposite trend. The correlation suggested that the labile fractions (MBC and POC) and inert fraction (MAOC) were significantly positively correlated with SOC (P<0.001) in the three land use types, while no significant correlations were found between DOC and SOC and its fractions (P>0.05). There was a significant positive correlation between POC and MBC in upland and woodland (P<0.001). POC was significantly positively correlated with MAOC in the three land use types (P<0.001). MAOC and MBC in paddy and upland were significantly positively correlated (P<0.001). Therefore, compared with upland and woodland, SOC in paddy had a higher proportion of labile SOC fraction, but a lower proportion of inert fraction. Moreover, MBC content in paddy was not related to the accumulation of the labile fraction of POC, but positively related to the accumulation of the inert fraction of MAOC. In summary, agricultural land uses have great influence on SOC and its fractions in hilly red soil regions. Though paddy is beneficial for SOC sequestration, the proportions of labile fractions in its SOC are relatively higher, and thus it is vulnerable to loss due to improper agricultural management.

A Coupled Extraction/Re-Extraction Method for the Chemical Speciation of Nickel in Natural Waters

Chemical fractionation and speciation of metals species in natural waters and its relation with bioavailability have received increased attention in recent years. A simple liquid membranes method, based on coupled liquid extraction and re-extraction processes, is proposed to separate and quantify the species of nickel present in water samples. A simplex optimization of chemical variables, such as carrier concentration in the organic solution and nitric acid concentration in the receiving solution, was performed and, under optimized conditions, the extraction system was applied to determine nickel species in water samples at natural level concentrations. A linear relationship was established between extraction efficacy and the concentration of dissolved organic carbon in the samples, allowing the separation and determination of labile and non-labile nickel fractions, since the latter was not transported through the organic solution acting as liquid membrane. When the total and labile concentrations of metals were analyzed in real samples with different salinities, no significant differences were found between the results obtained and those from well-established methods. An average relative error of 1.50 and 2.37 was obtained for total Ni concentration and labile fraction, respectively. Finally, a comparison with the theoretical speciation data calculated with the software WinHumic V was successfully performed. Thus, the proposed method allows the simultaneous determination of labile and non-labile nickel fractions, presented as a simple alternative to nickel fractionation in natural waters.