Non-enzymatic Electrochemical Glucose Sensor Research Articles

Non-enzymatic Glucose Sensor Based on Ni(OH)2/NF Materials Using Different Pulse Voltammetry Technique

Non-enzymatic glucose sensors have gained significant attention owing to their potential for accurate and cost-effective glucose detection. Nanomaterials play a pivotal role in enhancing performance of non-enzymatic glucose sensor. In this study, a novel non-enzymatic glucose sensor based on Ni(OH)2/NF (nickel hydroxide/nickel foam) materials was developed using different pulse voltammetry technique. Chemical precipitation methods have been used to grow Ni(OH)2 nanostructures directly on a nickel foam electrode. The characterization of the synthesized materials was performed through field emission scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDX), and X-ray diffraction (XRD). The performance of the Ni(OH)2/NF-based glucose sensor was evaluated through electrochemical measurements. Nanoscale hive-like structures of Ni(OH)2 with a diameter ranging from 450 to 530 μm and a thickness of 20 nm were formed on the NF surface. The sensor exhibited good performance, displaying a high sensitivity of 8.42mA.mM-1.cm-2 with a low detection limit of 0.84 μM. These results position the synthesized electrode as a promising contender for non-enzymatic electrochemical glucose sensors.

Tailoring the d-band center on Ru1Cu single-atom alloy nanotubes for boosting electrochemical non-enzymatic glucose sensing.

The development of cost-effective and highly efficient electrocatalysts is critical to help electrochemical non-enzymatic sensors achieve high performance. Here, a new class of catalyst, Ru single atoms confined on Cu nanotubes as asingle-atom alloy (Ru1Cu NTs), with aunique electronic structure and property, was developed to construct a novel electrochemical non-enzymatic glucose sensor for the first time. The Ru1Cu NTs with a diameter of about 24.0nm showed amuch lower oxidation potential (0.38V) and 9.0-fold higher response (66.5 μA) current than Cu nanowires (Cu NWs, oxidation potential 0.47V and current 7.4 μA) for glucose electrocatalysis. Moreover, as an electrochemical non-enzymatic glucose sensor, Ru1Cu NTs not only exhibited twofold higher sensitivity (54.9 μA mM-1cm-2) and wider linear range (0.5-8mM) than Cu NWs, but also showed alow detection limit (5.0μM), excellent selectivity, and great stability. According to theoretical calculation results, the outstanding catalytic and sensing performance of Ru1Cu NTs could be ascribed to the upshift of the d-band center that helped promote glucose adsorption. This work presents a new avenue for developing highly active catalysts for electrochemical non-enzymatic sensors.

Synergistically enhancing electrocatalysis and non-enzymatic sensing for glucose by iridium single-atom/nickel oxide/N-doped graphene.

The development of novel catalyst with high catalytic activity is important for electrochemical non-enzymatic glucose sensing. Here, iridium single-atom/nickel oxide nanoparticle/N-doped graphene nanosheet (Ir1/NiO/NG) with the loading of 1.13 wt% Ir was successfully synthesized for constructing electrochemical non-enzymatic glucose sensor for the first time. The morphology and structure of Ir1/NiO/NG were characterized by XRD, SEM, TEM, HRTEM, and XPS, and the presence of Ir SAs was confirmed by AC-HAADF-STEM. The Ir1/NiO/NG shows 65 mV lower oxidation potential and 3.3 times higher response current than Ni(OH)2/NG. In addition, Ir1/NiO/NG exhibits high sensitivity (70.09 μA mM-1 cm-2), excellent selectivity, low detection limit (2.00 μM), and great stability (91.53% current remaining after 21 days) for electrochemical non-enzymatic glucose sensing. The outstanding catalytic and sensing performance of Ir1/NiO/NG is mainly attributed to synergistic effect of Ir SAs, NiO nanoparticles, and highly conductive NG, which modulate the electronic and geometric structure of Ir1/NiO/NG. This work shows the promising potential of SACs in electrochemical sensing.

Hollow nanocages heterostructured NiCo-LDH/MWCNTs electrocatalyst for highly sensitive and non-invasive detection of saliva glucose

Monitoring salivary glucose has gained increased attention as a non-invasive approach to prevent and diagnose diabetes. Since the glucose concentration in saliva is lower than that in blood, the development of effective electrocatalysts for highly sensitive glucose detection is thus of great significance. In this study, a hollow nanocage from NiCo-layered double hydroxides (LDH) modified with multi-walled carbon nanotubes (MWCNTs) is designed and further employed as the sensing material to construct a novel non-enzymatic glucose electrochemical sensor with high sensitivity. It is synthesized by utilizing pre-synthesized zeolitic imidazolate framework (ZIF-67)/MWCNTs as a sacrificial template and subsequently conducting a simple solvent-thermal reaction with Ni(NO3)2. Such a hollow nanocage preserves the polyhedral framework structure of the used precursor, where the hollow frame-like structure of NiCo-LDH offers accessible catalytic sites, and MWCNTs provides good conductivity. Their combination brings in a bridging effect between MWCNTs and NiCo-LDH, further leading to a large electrochemical active area and excellent catalytic activity of this hollow nanocage. The constructed sensor exhibits a detection limit as low as 0.03 μM for the glucose detection as well as wide linear ranges of 0.1–3000 μM and 3000–9231.8 μM, corresponding to the sensitivity of 2.55 and 1.15 μA mM−1 cm−2, respectively. Moreover, this sensor enables the tracking of glucose concentration change in salivary before and after food intake. This work offers new highly sensitive sensing materials and potentially valuable approaches for non-invasive glucose detection.

Construction of hierarchical NiCo2 S4 /2D-Carbyne nanohybrid onto nickel foam for high performance supercapacitor and non-enzymatic electrochemical glucose sensor applications.

Synthesising and designing pseudocapacitive material with good electrochemical and electrocatalytic behaviour is essential to use as supercapacitor as well as non-enzymatic glucose sensor electrode. In this work, NiCo2 S4 nanoparticles decorated onto the 2D-Carbyne nanosheets are achieved by the solvothermal process. The as-prepared NiCo2 S4 @2D-Carbyne provides rich reaction sites and better diffusion pathways. On usage as an electrode for supercapacitor application, the NiCo2 S4 @2D-Carbyne exhibits the specific capacitance of about 2507 F g-1 at 1 A g-1 . In addition, the fabricated hybrid device generates an energy density of 52.2 Wh kg-1 at a power density of 1.01 kW kg-1 . Besides, the glucose oxidation behaviour of NiCo2 S4 @2D-Carbyne modified GCE has also been performed. The diffusion of glucose from the electrolyte to the electrode obeys the kinetic control process. Furthermore, the fabricated NiCo2 S4 @2D-Carbyne non-enzymatic glucose sensor exhibits a limit of detection of about 34.5 μM with a sensitivity of about 135 μA mM-1 cm-2 . These findings highlight the need to design and synthesis electrode materials with adequate electrolyte-electrode contact, strong structural integrity, and rapid ion/electron transport.

A novel NiO/C@rGO nanocomposite derived from Ni(gallate): A non-enzymatic electrochemical glucose sensor

The development of suitable substrate materials in electrochemical sensors is essential for rapid and reliable quantification in the diagnostic and clinical fields. In this paper, NiO/C@rGO nanocomposite was synthesized through nickel (gallate) pyrolysis with graphene oxide and employed as a substrate to construct a glucose sensor with high selectivity and sensitivity. The structural characterizations of NiO/C@rGO were assessed by Fourier transform infrared spectroscopy, X-ray diffraction, field emission scanning electron microscopy, energy disperse spectroscopy, and elemental mapping. The newly fabricated biosensor (NiO/C@rGO/GCE) exhibited a high sensing performance with an extensive dynamic linear range (from 1 μM to 1115 μM) and a low limit of detection (LOD) 0.658 μM (S/N = 3) toward glucose. In addition, the NiO/C@rGO-based sensor displayed good stability, favorable repeatability, and anti-interference ability for determining glucose. It was also used to measure glucose in serum samples, and satisfactory results were attained. These results illustrated that the NiO/C@rGO nanocomposite could be applied as an excellent electrochemical sensing material with high sensitivity to diagnose glucose-related illnesses.

Hydrothermally synthesized CuNiS@CNTs composite electrode material for hybrid supercapacitors and non-enzymatic electrochemical glucose sensor

Hydrothermally synthesized CuNiS@CNTs composite electrode material for hybrid supercapacitors and non-enzymatic electrochemical glucose sensor

A novel non-enzymatic electrochemical glucose sensors based on graphene oxide nanoribbons: Tracking energy expenditure and nutritional intake in sports

This work revealed the development of a novel non-enzymatic electrochemical sensor for glucose detection based on a modified glassy carbon electrode made of graphene oxide nanoribbons (GONRs). The TEM, XRD), XPS, FTIR, and Raman spectroscopy were used to characterize the shape and structure of the modified electrode made of GONRs. Using amperometry and cyclic voltammetry (CV), the nanocomposite's electrochemical performance was assessed (AMP). The TEM results support the XPS, Raman, and XRD observations, demonstrating the capability of the suggested approach to generate single-layered GONRs with good edge linearity. The suggested non-enzymatic glucose sensing method was found to be selective, stable, repeatable, and reproducible through electrochemical analyses. The response behavior of GONRs/GCE was found to be linear within the 0.1–105 mM range, and the sensitivity and detection limit were found to be roughly 20.815 µA/mM and 3 µM, respectively. To verify the GONRs/GCE sensor's practicality and viability, tests were conducted on human serum and orange juice samples. The recovery rate ranged from 94.00% to 99.20%, according to the results, and the relative-standard deviation (RSD) values were 3.21% to 4.41%. These encouraging RSD and recovery rates showed that GONRs/GCE would work well as electrodes for measuring glucose in actual samples.

Printing surface cuprous oxides featured liquid metal for non-enzymatic electrochemical glucose sensor

Electrochemical glucose sensors that rely on two-dimensional (2D) oxides have attracted significant attention owing to the strong sensing activity of 2D oxides, but their practical application is hindered by the complexity and high cost of fabrication of electrodes and integrated devices. Herein, a convenient and effective fabrication route that includes printing a Ga-based liquid metal (LM) as a current collection electrode, followed by growing electrochemically active 2D oxides directly on the surface of Ga-based LMs under mild conditions, is developed for non-enzyme-based electrochemical sensors. Specifically, 2D annealed Cu-Oxide (ACO) is successfully grown on a printed Ga electrode through a galvanic replacement reaction, resulting in the formation of a mechanically and electrically well-matched interface between the active sensing materials and the current collection substrate. Benefitting from the high quantity of 2D ACO and good charge transfer at the interface, the as-prepared ACO electrode exhibits attractive glucose sensing performance, with a wide linear range (1 μM-10 mM) of effective detection, low detection limit down to 1 μM, and high sensitivity of 0.87 μA·mM-1·cm-2. Our study highlights the potential of using LMs in bio-sensing applications and provides a non-enzyme-based electrochemical biosensor platform for effective glucose detection in diets and clinical diagnostic settings.

Green synthesized gold nanoparticles and CuO-based nonenzymatic sensor for saliva glucose monitoring.

Glucose, essential for brain and muscle functions, requires careful monitoring in diabetes and other chronic disease management. While blood glucose monitoring provides precise information about these diseases, it remains an invasive method. Saliva glucose monitoring could offer an alternative approach, but the glucose concentration in saliva is very low. In this work, we report a simple, low-cost, highly sensitive nonenzymatic electrochemical glucose sensor. We developed this sensor using green synthesized gold nanoparticles (AuNPs) and wet chemical synthesized copper oxide (CuO) nanoparticles on a screen-printed carbon electrode (Au/CuO/SPCE). The sensor's high sensitivity results from dual amplification strategies using AuNPs and CuO nanomaterials, each demonstrating catalytic activity towards glucose. This shows promising potential for saliva glucose monitoring. The AuNPs were synthesized using an Au precursor and orange peel extract (OPE), yielding stable colloidal AuNPs with a mean diameter of about 37 nm, thus eliminating the need for additional capping agents. Under optimal conditions, amperometric tests revealed that the sensor responded linearly to glucose concentrations ranging from 2 μM to 397 μM with a sensitivity of 236.70 μA mM-1 cm-2. Furthermore, the sensor demonstrated excellent reproducibility, stability and high selectivity for glucose in the presence of different biomolecules. We validated the sensor's efficacy by measuring glucose in human saliva, showing its potential for noninvasive glucose monitoring. This research advances the development of point-of-care devices, positioning the sensor as a promising tool for noninvasive glucose monitoring and improved diabetes management.

One-Step Method for the Direct Growth of NiO Nanoflowers on Pencil Graphite Electrode for Highly Sensitive Non-Enzymatic Glucose Sensing

Electrochemical glucose sensors, including enzymatic and non-enzymatic sensors, have been considered as quick, accurate, and efficent means for glucose measurement. Recently, non-enzymatic electrochemical glucose sensor based on metal oxides have been paid attention to develop thank to their outstanding behaviours. In most of these studies, metal oxides were synthesized individually and subsequently deposited on the electrode surface using a binder. In this study, we aim at developing a non-enzymatic glucose sensor based on NiO nanoflowers directly grown on a commercial pencil graphite electrode. NiO matetials were deposited on the electrode without a binder via a facile and low-cost chemical procedure. The synthesised materials’ morphologies and components of were characterized by a field emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray diffraction analyses. Electrocatalytic performance was recorded by cyclic voltammetry and chronoamperometry. The resulting NiO nanoflowers, characterized by funnel-shaped structures measuring 500–600 nm in diameter and 20 nm in thickness, were formed on the pencil graphite electrode. The fabricated electrode showed a remarkably high sensitivity of 337 μA mM−1 cm−2, a low limit of detection of 4 μM, and good selectivity. The fabricated electrode can be a potential candidate for clinically medical applications.

Green synthesis of reduced graphene oxide (rGO) and its applications in non-enzymatic electrochemical glucose sensors

The synthesis of reduced graphene oxide (rGO) is a challenging task for industrial applications due to the absence of a simple and cost-effective synthesis method. This study proposes an environmentally friendly approach to synthesizing rGO using cow urine as a reducing agent, which eliminates the use of hazardous chemicals. The efficacy of this method was confirmed through various analyses, including UV Vis, Fourier transform infrared, and X-ray diffraction, which demonstrated the successful removal of oxygen-containing functions in GO via reduction with cow urine. The synthesized rGO-10 was utilized to fabricate electrochemical nonenzymatic glucose sensors, which exhibited a rapid response time and excellent amperometry response. The sensors used in this study demonstrated a sensitivity of 19.17 μAcm−2mM−1 when optimized for a glucose concentration of 2 mM in human serum. The sensors demonstrated a wide response range, covering a range of 0.2 to 10 mM, and a low detection limit of 1.9019 µM. Additionally, the sensors displayed desirable attributes about repeatability, reproducibility, and stability.

One-step hydrothermal synthesis of CuS/MoS2 composite for use as an electrochemical non-enzymatic glucose sensor

Early diagnosis may be crucial for the prevention of chronic diabetes mellitus. For that herein, we prepared a CuS/MoS2 composite for a non-enzymatic glucose sensor through a one-step hydrothermal method owing to the synergetic effect of CuS/MoS2. The surface morphology of CuS/MoS2 was studied by Field Emission Scanning Electron Microscopy (FESEM) and Cs-corrected Scanning Transmission Electron Microscopy (Cs-STEM). The crystallinity and surface composition of CuS/MoS2 were analyzed by X-ray Diffraction (XRD) and X-ray Photoelectron Spectroscopy (XPS) respectively. The working electrode was prepared from CuS/MoS2 electrocatalyst, and for that dispersed solution of electrocatalyst was used to fabricate the material-loaded glassy carbon electrode (GC). CuS/MoS2 composite shows the viability of electrocatalyst to oxidize glucose in an alkaline solution with sensitivity and detection limit of 252.71 μA mM−1 cm−2 and 1.52 μM respectively. The proposed glucose sensor showed reasonable stability and potential selectivity during electrochemical analysis. Accordingly, the CuS/MoS2 composite has potential as a viable material for glucose sensing in diluted human serum.

Non-enzymatic glucose electrochemical sensor based on nitrogen-doped graphene modified with polyaniline and Fe3O4@MIL-101-NH2 nano framework

In this study, a novel highly sensitive and selective non-enzymatic glucose sensor was fabricated by coating simple and cheap graphite sheet (GS) electrodes with a proper conductive nanocomposite containing nitrogen-doped functionalized graphene (NFG), conductive polyaniline (PANI), and core–shell nanoparticles (Fe3O4@MIL-101-NH2). The results revealed that the core centers of iron oxide nanoparticles shelled by MIL-101-NH2 enhanced the electron transfer and led to more conductivity, also their existence caused the limitation of the MOF’s low conductivity. In addition, using Fe3O4@MIL-101-NH2 in the nanocomposite structure led to more sensitivity and selectivity at glucose monitoring due to its amine groups and unique structure. The results showed that NFG/PANI/Fe3O4@MIL-101-NH2 nanocomposite provided a large surface area besides enhanced electron transfer and catalytic properties that lead to proper oxidation of glucose with two linear ranges of 0.5 – 10 µM and 100 µM – 25 mM and a low detection limit of 0.3 µM (S/N = 3). Furthermore, the relative standard deviation (RSD%) in all detection processes was lower than 9 %. Finally, the performance of fabricated non-enzymatic sensor (GS/NFG/PANI/Fe3O4@MIL-101-NH2) in human fluids real samples including serum and plasma was acceptable which significantly indicates that it is toward monitoring of glucose in clinical applications.

Electrochemical nonenzymatic glucose sensors catalyzed by Au nanoclusters on metallic nanotube arrays and polypyrrole nanowires

Monitoring blood glucose level is critical, since its abnormality leads to diabetes and causes death, even though glucose is essential for human living. Herein, the sensing study was performed on electrochemical nonenzymatic glucose sensors, which are composed of an Au nanocluster (AuNC) catalyst deposited on a metallic nanotube array (MeNTA) and polypyrrole nanowire (PPyNW). The AuNC was produced by irradiating a femtosecond pulse laser to the Au precursor solution, and it is a simple and facile method. The successful deposition of AuNC on both MeNTA and PPyNW was confirmed by means of the surface morphology and the Au content increase. On the exploration by cyclic voltammetry in alkaline condition, AuNC/MeNTA electrodes showed better performance than AuNC/PPyNW electrodes: The former was a remarkable electrocatalytic detector towards glucose oxidation with better sensitivity, lower detection limit, wider linear range, and longer-term stability without interference from potential interfering agents such as ascorbic acid, urea, NaCl, KCl, etc. Moreover, nonenzymatic AuNC/MeNTA electrodes exhibited high precision and accuracy in real human blood samples and, thus, can be a promising candidate in glucose sensing applications.

A Highly Sensitive Non-Enzymatic Glucose Electrochemical Sensor Electrode Material of CuO Nanospheres/Activated Carbon Composites

A Highly Sensitive Non-Enzymatic Glucose Electrochemical Sensor Electrode Material of CuO Nanospheres/Activated Carbon Composites

Recent advances in electrochemical non-enzymatic glucose sensor for the detection of glucose in tears and saliva: A Review

Recent advances in electrochemical non-enzymatic glucose sensor for the detection of glucose in tears and saliva: A Review

Copper nanoparticles/polyaniline/molybdenum disulfide composite as a nonenzymatic electrochemical glucose sensor

A Cu@Pani/MoS2 nanocomposite was successfully synthesized via combined in-situ oxidative polymerization and hydrothermal reaction and applied to an electrochemical nonenzymatic glucose sensor. The morphology of the prepared Cu@Pani/MoS2 nanocomposite was characterized using FE-SEM and Cs-STEM, and electrochemical analysis was performed using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and chronoamperometry techniques. Electrostatic interaction between Cu@Pani and MoS2 greatly enhanced the charge dispersion, electrical conductivity, and stability, resulting in excellent electrochemical performance. The Cu@Pani/MoS2 was used as an electrocatalyst to detect glucose in an alkaline medium. The proposed glucose sensor exhibited a sensitivity, detection limit, and wide linear range of 69.82 μAmM−1cm−2, 1.78 μM, and 0.1–11 mM, respectively. The stability and selectivity of the Cu@Pani/MoS2 composite for glucose compared to that of the potential interfering species, as well as its ability to determine the glucose concentration in diluted human serum samples at a high recovery percentage, demonstrated its viability as a nonenzymatic glucose sensor.

NiCo2O4/Ti2NbC2 (double MXene) nanohybrid-based non-enzymatic electrochemical biosensor for the detection of glucose in sweat.

The non-enzymatic electrochemical sensors are attractive due to their high sensitivity, quick detection, low cost, and simple construction. Hence, in this work, a non-enzymatic biosensor was constructed with NiCo2O4 nanoparticles (~ 82nm) decorated over Ti2NbC2 nanosheets by an in-situ method. The crystal structure, phase purity, morphology and elemental composition of the synthesized NiCo2O4/Ti2NbC2 nanohybrid was investigated using XRD, Raman and FESEM analysis. The electrocatalytic and electrochemical behaviour of the prepared nanohybrid was investigated using cyclic voltammetry and amperometry analysis. Hybrid of NiCo2O4/Ti2NbC2 produces a biocompatible, electrochemically active surface with enhanced electrical conductivity. The enhanced surface area of NiCo2O4 and superior electrical conductivity of Ti2NbC2 nanosheets helped to develop non-enzymatic electrochemical glucose sensor with enhanced sensitivity (425.6 µA mM-1cm-2), low limit of detection and quick response time that satisfy glucose detection applications. Thus, the developed non-enzymatic electrochemical glucose sensor has excellent electrochemical properties and making it as a strong candidate for the detection of glucose concentration in sweat.

One-pot hydrothermal synthesis of CuS/CoS composite for electrochemical non-enzymatic glucose sensor

Herein, a CuS/CoS electrode material was synthesized using a one-step hydrothermal method as an efficient electrocatalyst for a non-enzymatic glucose sensor. The nanocomposites were characterized by Field emission scanning electron microscope (FE-SEM), Transmission electron microscope (TEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The CuS/CoS composite loaded glassy carbon electrode was used to investigate electrochemical properties using cyclic voltammetry (CV) and chronoamperometry (CA). The results showed 314.85 μA mM−1 cm−2 sensitivity and 1.71 μM detection limit. Selectivity of the sensor was examined over potential interfering species that exhibits the distinct increased current response on addition of glucose before and after addition of interfering species evident the selectivity of CuS/CoS electrode material. Moreover, the CuS/CoS composite-based non-enzymatic glucose sensor revealed considerable stability, selectivity over interfering species, and viability for glucose determination in diluted human serum samples, which provide efficient and cost-effective strategy to develop non-enzymatic electrochemical glucose sensing platform.