TIAR, is a nucleic acid binding protein involved in the formation of cytoplasmic foci known as stress granules, in which mRNA translation is temporarily blocked in response to stressful conditions. TIAR is used as stress granules molecular marker in vertebrates, but it is not so deeply investigated in invertebrates, especially in marine organisms. In the present work, we investigated the role of TIAR in the colonial ascidian Botryllus schlosseri during its non-embryonic development, featured by the cyclical renewal of the colony. We studied the extent of transcription during the colonial blastogenetic cycle and the location of the transcripts in Botryllus tissues. Using an anti-TIAR antibody specific for ascidians, by immunocytochemistry and immunohistochemistry assays, we studied the expression of the protein in haemolymph cells and body tissues and by transmission electron microscopy we identified its subcellular localisation. The anti-TIAR antibody was also microinjected in the circulatory system of B. schlosseri to study its effect on non-embryonic development and immune responses. Results indicate a delay in the progression of the blastogenetic cycle in injected colonies. In addition, degranulation of circulating cytotoxic cells and phagocytosis by professional, circulating phagocytes, two fundamental processes of innate immunity, were also negatively affected.