Plant protection based solely on modern fungicides could lead to genetic changes in neurons of animal and humans that contribute to cases of autism and Alzheimer disease, unless the bio control agents is applied or replaced. Most researches is focused on the strongest chemical fungicides, dangerous to human health, for effective plant disease control and we believe that non-chemical control methods such as biological agents like AMF are of great importance. The aim of study was to investigate whether the soil inoculation with Glomus isolates of arbuscular mycorrhizal fungi (AMF) affected control of powdery mildew disease of apple MM111 and its survival and growth instead of use of chemical fungicides such as Flint and Stroby. Twenty apple seedlings were randomly arranged to 4 treatments, each with 5 replicates (T1, control = no AMF, no fungicide, T2= Flint fungicide, T3= Stroby fungicide and T4 = only AMF mixture) and were monitored throughout 9 weeks. All seedlings were exposed to powdery mildew on week 6 and only T2 and T3 plants treated by fungicides after developing mildew colonies on the leaves. Results showed that, seedling length in plants cultivated in AMF-inoculated-soil was significantly higher than other treatments especially in weeks 1-4 and weeks 6-9. Leaf growth rate of all plants during the experimental growth period non-significantly increased between treatments with the exception of first week that did show a significant increase in leaf growth rate of group 4 plants, even after exposure to disease. T4 samples showed a high average of leaves numbers (P < 0.05) in compared to other groups followed by T3 samples during the experimental growth period. The data from this study confirmed the response of seedling and leaf growth rates of apple seedlings to mycorrhizal colonization. It was concluded that plants cultivated in soil inoculated to AMF throughout 6 weeks had higher resistance and growth rates against Podosphaera leucotricha fungi as an agent of powdery mildew disease in apple seedling and it can be considered as an applicable strategy in biocontrol measures against pathogens when most researches is focused on chemical fungicides.