IntroductionThe probability density function (PDF) of the surface electromyogram (sEMG) depends on contraction force. This dependence, however, has so far been investigated by having the subject generate force at a few fixed percentages of MVC. Here, we examined how the shape of the sEMG PDF changes with contraction force when this force was gradually increased from zero.MethodsVoluntary surface EMG signals were recorded from the vastus lateralis of healthy subjects as force was increased in a continuous manner vs. in a step-wise fashion. The sEMG filling process was examined by measuring the EMG filling factor, computed from the non-central moments of the rectified sEMG signal.Results(1) In 84% of the subjects, as contraction force increased from 0 to 10% MVC, the sEMG PDF shape oscillated back and forth between the semi-degenerate and the Gaussian distribution.(2) The PDF–force relation varied greatly among subjects for forces between 0 and ~ 10% MVC, but this variability was largely reduced for forces above 10% MVC.(3) The pooled analysis showed that, as contraction force gradually increased, the sEMG PDF evolved rapidly from the semi-degenerate towards the Laplacian distribution from 0 to 5% MVC, and then more slowly from the Laplacian towards the Gaussian distribution for higher forces.ConclusionsThe study demonstrated that the dependence of the sEMG PDF shape on contraction force can only be reliably assessed by gradually increasing force from zero, and not by performing a few constant-force contractions. The study also showed that the PDF–force relation differed greatly among individuals for contraction forces below 10% MVC, but this variability was largely reduced when force increased above 10% MVC.