Non-cellulosic Polysaccharides Research Articles

Influence of infection with Brachyspira hyodysenteriae on clinical expression, growth performance, and digestibility in growing pigs fed diets varying in type and level of fiber.

Research on the effects of different fiber types and levels on infection with Brachyspira hyodysenteriae on growth performance and nutrients digestibility in pigs is scarce. The objective of the current study was to investigate the effects of infection with B. hyodysenteriae when feeding diets varying in soluble and insoluble dietary fiber (DF) on the expression of swine dysentery, growth performance, and digestibility of organic matter (OM) nutrients. A total of 96 growing pigs (26.9 ± 2.5 kg) were used for the experiment and divided into six blocks. The growing pigs were fed one of four diets for 12 wk: low fiber (LF), high fiber (HF), high soluble fiber (HS), and high insoluble fiber (HI). After 2 wk, half of the pigs were inoculated with B. hyodysenteriae. Half of the pigs in each group were euthanized at week 6 for the measurement of the apparent digestibility at the ileum, cecum, colon, and total tract. The remaining pigs were maintained to observe and analyze the clinical expression of fecal score and excretion of B. hyodysenteriae, growth performance, and total tract digestibility up to 12 wk. In the current study, the experimental diets did not influence the expression of infection in the pigs. The body weight and average daily gain (ADG) were in line with the results of clinical expression from week 4 to 6. However, the ADG of the infected pigs started to recover from week 6 (P < 0.05) and then recovered from week 8 to 12 (P < 0.05). The infection with B. hyodysenteriae did not impair apparent ileal digestibility (AID; P > 0.05), whereas the apparent digestibility of OM, total non-starch polysaccharide, non-cellulosic polysaccharide, and cellulose in the cecum of the infected pigs was higher than non-infected pigs (P < 0.05). The apparent colonic digestibility of ash and nitrogen was higher in non-infected pigs than in infected pigs (P < 0.05). The pigs fed the LF diet had a higher digestibility in all segments of the intestinal tract, whereas the HS diet had the lowest AID but higher or similar to the LF diet in the cecum, colon, and the total tract (P < 0.05). The pigs fed the HF and HI diets, with a high proportion of insoluble fiber, had a lower digestibility in the hindgut than the other two diets (P < 0.05). In conclusion, the infection with B. hyodysenteriae negatively influenced clinical signs of swine dysentery and growth performance but did not impair AID, and neither soluble nor insoluble DF influenced the expression of the infection.

MODIFYING THE FUNCTIONALITIES OF DIETARY FIBER IN FOODS BY VARIOUS PROCESSING METHODS

Dietary fiber is that part of plant material in the diet which is resistant to enzymatic digestion which includes cellulose, non-cellulosic polysaccharides such as hemicellulose, pectic substances, gums, mucilages and a non-carbohydrate component lignin. The diets rich in fiber such as cereals, nuts, fruits and vegetables have a positive effect on health since their consumption has been related to decreased incidence of several diseases. Dietary fiber can be used in various functional foods like bakery, drinks, beverages and meat products. Influence of different processing treatments (like extrusioncooking, canning, grinding, boiling, frying) alters the physico- chemical properties of dietary fiber and improves their functionality. An attempt has been made to compile the recent developments in the extraction, applications and functions of dietary fiber in different food products

Can lignin and monomer composition of fibre describe the variation in iNDF in forages?

Indigestible NDF (iNDF) is an entity in feed evaluation systems. In this study, iNDF is defined as the portion of NDF not digested after 288 h of rumen incubation in Dacron bags with 12 µm pore size. However, the method is resource demanding. The objective of this study was to elucidate the effect of lignin and hemicellulose monomers on iNDF variation within and among forage types and their ability to predict iNDF variation in linear and multiple regression models. The hypotheses were 1) lignin is expected to have high ability in describing the iNDF variation and 2) the inclusion of hemicellulose monomers will increase this ability. iNDF was determined on 50 grass-clover silages and 50 whole crop maize silages as well as 40 maize stem and 10 maize ear samples. All samples were analysed for neutral detergent fibre (NDF), Klason lignin (KL), cellulose and non-cellulose polysaccharides (NCP). Grass-clover and whole crop maize silages were analysed for acid detergent lignin (ADL). Lignin variables had the most consistent correlation to the iNDF variation throughout both correlation and regression analyses. Arabinose also showed high correlations and relationships in linear regression to iNDF and almost at same level as lignin variables. Multiple linear regression increased the accuracy for grass-clover silage and combined analysis with all forage types, with KL and/or ADL as first explanatory variables. However, the increase in accuracy is not consistent between forage types.

Immune Priming Triggers Cell Wall Remodeling and Increased Resistance to Halo Blight Disease in Common Bean.

The cell wall (CW) is a dynamic structure extensively remodeled during plant growth and under stress conditions, however little is known about its roles during the immune system priming, especially in crops. In order to shed light on such a process, we used the Phaseolus vulgaris-Pseudomonas syringae (Pph) pathosystem and the immune priming capacity of 2,6-dichloroisonicotinic acid (INA). In the first instance we confirmed that INA-pretreated plants were more resistant to Pph, which was in line with the enhanced production of H2O2 of the primed plants after elicitation with the peptide flg22. Thereafter, CWs from plants subjected to the different treatments (non- or Pph-inoculated on non- or INA-pretreated plants) were isolated to study their composition and properties. As a result, the Pph inoculation modified the bean CW to some extent, mostly the pectic component, but the CW was as vulnerable to enzymatic hydrolysis as in the case of non-inoculated plants. By contrast, the INA priming triggered a pronounced CW remodeling, both on the cellulosic and non-cellulosic polysaccharides, and CW proteins, which resulted in a CW that was more resistant to enzymatic hydrolysis. In conclusion, the increased bean resistance against Pph produced by INA priming can be explained, at least partially, by a drastic CW remodeling.

Biosorption of heavy metal ions by green alga Neochloris oleoabundans: Effects of metal ion properties and cell wall structure

Effects of metal ion proprieties and the cell wall structure of green alga Neochloris oleoabundans were investigated on five strategically selected heavy metal ions, Pb(II), Hg(II), Zn(II), Cd(II) and Cu(II). The biosorption of these ions were energy-independent and spontaneous Langmuir adsorption. The adsorption capacities of Pb(II), Hg(II), Zn(II), Cd(II) and Cu(II) were determined to be 1.03, 0.91, 1.20, 0.65 and 1.23 mmol/g, respectively. Data suggest that peptide-containing molecules and non-cellulosic polysaccharides on cell wall were the primary sites of adsorption. Ion Pb(II) showed the strongest inhibitive effects on the adsorption of other metal ions on cells in binaries, corresponding to its large affinity to the biosorbents, which was next only to that of Cu(II). A linear relation was established for the first time between the adsorption capacity and the impact factor, which is defined in this paper as the electronegativity of a metal ion normalized by its atomic radius. In other words, adsorption capacity of N. oleoabundans biomass to the tested two-valence metal ions is proportional to the electronegativity and inversely proportional to the radius of the metal ions. Cell aggregation was caused by the addition of Cu(II), which exhibited distinctive adsorption behaviors than other metal ions.

Fractionation of Lignocellulosic Biomass using the OrganoCat Process.

The shift from a petroleum-based to a more sustainable and bio-based economy requires the development of new refinery concepts to maintain the supply of raw materials and energy. For these novel and sustainable biorefinery concepts, it is important to use catalysts and solvents that are aligned with the principles of Green Chemistry. Therefore, the implementation of biogenic alternatives can be a promising solution. The lignocellulose pretreatment and fractionation process presented herein-OrganoCat-is an integrated fractionation of lignocellulose into its main components using biogenic acids such as 2,5-furandicarboxylic acid as catalyst. Hemicelluloses and other non-cellulosic polysaccharides are selectively depolymerized by the diluted acid and dissolved, while the crystalline cellulose remains in the solid pulp. In the presence of a second organic phase consisting of biogenic 2-methyltetrahydrofuran, disentangled lignin is extracted in situ. The process allows for the efficient fractionation of the three main components-lignin, cellulose, and non-cellulosic sugars. This helps to focus on the quality of the lignin, the improvement of enzymatic hydrolysis of the cellulose-enriched pulp, and the mild non-cellulosic sugar extraction with low degradation.

Fractionation of Lignocellulosic Biomass using the OrganoCat Process

The shift from a petroleum-based to a more sustainable and bio-based economy requires the development of new refinery concepts to maintain the supply of raw materials and energy. For these novel and sustainable biorefinery concepts, it is important to use catalysts and solvents that are aligned with the principles of Green Chemistry. Therefore, the implementation of biogenic alternatives can be a promising solution. The lignocellulose pretreatment and fractionation process presented herein-OrganoCat-is an integrated fractionation of lignocellulose into its main components using biogenic acids such as 2,5-furandicarboxylic acid as catalyst. Hemicelluloses and other non-cellulosic polysaccharides are selectively depolymerized by the diluted acid and dissolved, while the crystalline cellulose remains in the solid pulp. In the presence of a second organic phase consisting of biogenic 2-methyltetrahydrofuran, disentangled lignin is extracted in situ. The process allows for the efficient fractionation of the three main components-lignin, cellulose, and non-cellulosic sugars. This helps to focus on the quality of the lignin, the improvement of enzymatic hydrolysis of the cellulose-enriched pulp, and the mild non-cellulosic sugar extraction with low degradation.

Semidry acid hydrolysis of cellulose sustained by autoclaving for production of reducing sugars for bacterial biohydrogen generation from various cellulose feedstock.

Cellulosic biowastes are one of the cheapest and most abundant renewable organic materials on earth that can be, subsequent to hydrolysis, utilized as an organic carbon source for several fermentation biotechnologies. This study was devoted to explore a semidry acid hydrolysis of cellulose for decreasing the cost and ionic strength of the hydrolysate. For semidry acid hydrolysis, cellulose was just wetted with HCl (0 to 7 M) and subjected to autoclaving. The optimum molar concentration of HCl and period of autoclaving for semidry acid hydrolysis of cellulose were 6 M and 50 min respectively. Subsequent to the semidry acid hydrolysis with a minimum volume of 6 M HCl sustained by autoclaving, the hydrolysate was diluted with distilled water and neutralized with NaOH (0.5 M). The reducing sugars produced from the semidry acid hydrolysis of cellulose was further used for dark fermentation biohydrogen production by Escherichia coli as a representative of most hydrogen producing eubacteria which cannot utilize non-hydrolyzed cellulose. An isolated E. coli TFYM was used where this bacterium was morphologically and biochemically characterized and further identified by phylogenetic 16S rRNA encoding gene sequence analysis. The reducing sugars produced by semidry acid hydrolysis could be efficiently utilized by E. coli producing 0.4 mol H2 mol−1 hexose with a maximum rate of hydrogen gas production of 23.3 ml H2 h−1 L−1 and an estimated hydrogen yield of 20.5 (L H2 kg−1 dry biomass). The cheap cellulosic biowastes of wheat bran, sawdust and sugarcane bagasse could be hydrolyzed by semidry acid hydrolysis where the estimated hydrogen yield per kg of its dry biomass were 36, 18 and 32 (L H2 kg−1 dry biomass) respectively indicating a good feasibility of hydrogen production from reducing sugars prepared by semidry acid hydrolysis of these cellulosic biowastes. Semidry acid hydrolysis could also be effectively used for hydrolyzing non-cellulosic polysaccharides of dry cyanobacterial biomass. The described semidry acid hydrolysis of cellulosic biowastes in this study might be applicable not only for bacterial biohydrogen production but also for various hydrolyzed cellulose-based fermentation biotechnologies.

Immunohistochemical analyses on two distinct internodes of stinging nettle show different distribution of polysaccharides and proteins in the cell walls of bast fibers

Stinging nettle is a perennial herbaceous species holding value as a multi-purpose plant. Indeed, its leaves and roots are phytofactories providing functional ingredients of medicinal interest and its stems produce silky and resistant extraxylary fibers (a.k.a. bast fibers) valued in the biocomposite sector. Similarly to what is reported in other fiber crops, the stem of nettle contains both lignified and hypolignified fibers in the core and cortex, respectively, and it is therefore a useful model for cell wall research. Indeed, data on nettle stem tissues can be compared to those obtained in other models, such as hemp and flax, to support hypotheses on the differentiation and development of bast fibers. The suitability of the nettle stem as model for cell wall-related research was already validated using a transcriptomics and biochemical approach focused on internodes at different developmental stages sampled at the top, middle, and bottom of the stem. We here sought to complement and enrich these data by providing immunohistochemical and ultrastructural details on young and older stem internodes. Antibodies recognizing non-cellulosic polysaccharides (galactans, arabinans, rhamnogalacturonans) and arabinogalactan proteins were here investigated with the goal of understanding whether their distribution changes in the stem tissues in relation to the bast fiber and vascular tissue development. The results obtained indicate that the occurrence and distribution of cell wall polysaccharides and proteins differ between young and older internodes and that these changes are particularly evident in the bast fibers.

Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues.

Plant development involves constant adjustments of the cell wall composition and structure in response to both internal and external stimuli. Cell walls are composed of cellulose and non-cellulosic polysaccharides together with proteins, phenolic compounds and water. 90% of the cell wall is composed ofpolysaccharides (e.g., pectins) and arabinogalactan proteins (AGPs). The fluorescent immunolocalization of specific glycan epitopes in plant histological sections remains a key tool to uncover remodeling of wall polysaccharide networks, structure and components. Here, we report an optimized fluorescent immunolocalization procedure to detect glycan epitopes from AGPs and pectins in plant tissues. Paraformaldehyde/glutaraldehyde fixation was used along with LR-White embedding of the plant samples, allowing for a better preservation of the tissue structure and composition. Thin sections of the embedded samples obtained with an ultra-microtome were used for immunolocalization with specific antibodies. This technique offers great resolution, high specificity, and the chance to detect multiple glycan epitopes in the same sample. This technique allows subcellular localization of glycans and detects their level of accumulation in the cell wall. It also permits the determination of spatio-temporal patterns of AGP and pectin distribution during developmental processes. The use of this tool may ultimately guide research directions and link glycans to specific functions in plants. Furthermore, the information obtained can complement biochemical and gene expression studies.

Structures, Biosynthesis, and Physiological Functions of (1,3;1,4)-β-D-Glucans.

(1,3;1,4)-β-d-Glucans, also named as mixed-linkage glucans, are unbranched non-cellulosic polysaccharides containing both (1,3)- and (1,4)-β-linkages. The linkage ratio varies depending upon species origin and has a significant impact on the physicochemical properties of the (1,3;1,4)-β-d-glucans. (1,3;1,4)-β-d-Glucans were thought to be unique in the grasses family (Poaceae); however, evidence has shown that (1,3;1,4)-β-d-glucans are also synthesized in other taxa, including horsetail fern Equisetum, algae, lichens, and fungi, and more recently, bacteria. The enzyme involved in (1,3;1,4)-β-d-glucan biosynthesis has been well studied in grasses and cereal. However, how this enzyme is able to assemble the two different linkages remains a matter of debate. Additionally, the presence of (1,3;1,4)-β-d-glucan across the species evolutionarily distant from Poaceae but absence in some evolutionarily closely related species suggest that the synthesis is either highly conserved or has arisen twice as a result of convergent evolution. Here, we compare the structure of (1,3;1,4)-β-d-glucans present across various taxonomic groups and provide up-to-date information on how (1,3;1,4)-β-d-glucans are synthesized and their functions.

Interactions between Cellulose and (1,3;1,4)-β-glucans and Arabinoxylans in the Regenerating Wall of Suspension Culture Cells of the Ryegrass Lolium multiflorum.

Plant cell walls (PCWs) form the outer barrier of cells that give the plant strength and directly interact with the environment and other cells in the plant. PCWs are composed of several polysaccharides, of which cellulose forms the main fibrillar network. Enmeshed between these fibrils of cellulose are non-cellulosic polysaccharides (NCPs), pectins, and proteins. This study investigates the sequence, timing, patterning, and architecture of cell wall polysaccharide regeneration in suspension culture cells (SCC) of the grass species Lolium multiflorum (Lolium). Confocal, superresolution, and electron microscopies were used in combination with cytochemical labeling to investigate polysaccharide deposition in SCC after protoplasting. Cellulose was the first polysaccharide observed, followed shortly thereafter by (1,3;1,4)-β-glucan, which is also known as mixed-linkage glucan (MLG), arabinoxylan (AX), and callose. Cellulose formed fibrils with AX and produced a filamentous-like network, whereas MLG formed punctate patches. Using colocalization analysis, cellulose and AX were shown to interact during early stages of wall generation, but this interaction reduced over time as the wall matured. AX and MLG interactions increased slightly over time, but cellulose and MLG were not seen to interact. Callose initially formed patches that were randomly positioned on the protoplast surface. There was no consistency in size or location over time. The architecture observed via superresolution microscopy showed similarities to the biophysical maps produced using atomic force microscopy and can give insight into the role of polysaccharides in PCWs.

The physiochemical alteration of flax fibers structuring components after different scouring and bleaching treatments

Scouring and bleaching are essential steps in flax yarn production; however, in many cases, these treatments have relatively low selectivity on non-cellulosic polysaccharides (NCPs) and lignin. Herein, the specific chemical properties of flax fibers through a variety of scouring and/or bleaching procedures are reviewed to understand their correlations with the mechanical and morphological characteristics. The hydrolysis behaviors of hemicellulose and lignin are different from those of cellulose during chlorine-alkali-oxygen (Cl-AO), enzyme-alkali-oxygen (EAO), and alkali-oxygen (AO) treatments, while the structural hemicellulose polysaccharides fractions were not affected by acid pretreatment followed by alkali scouring (AA) treatment. It was found that the unbleached AA treatment improved scouring efficiency to the greatest extent by decreasing 73.3 % of hemicellulose content and 59.8 % of lignin content compared to the untreated flax fibers and obtained the highest tensile strength (6.58 cN/dtex) and elongation (3.73 %). Delignification was more crucial on fiber tenacity and fineness than hemicellulose removal (R2 = 0.71, 0.79 vs R2 = 0.46, 0.61). Within the hemicellulose fractions, a corresponding beneficial relationship between the xylan (XYL) composition and flax fiber properties is presented. The correlation of GM and GGM contents in hemicellulose with flax fiber length was significant with R2 value 0.97 and 0.99, respectively. This study provides insight into the hydrolysis behavior of hemicellulose and a basis for developing scouring and/or bleaching models that can predict fiber properties.

Biomass composition of the golden tide pelagic seaweeds Sargassum fluitans and S. natans (morphotypes I and VIII) to inform valorisation pathways

Massive strandings of the pelagic brown algae Sargassum have occurred in the Caribbean, and to a lesser extent, in western Africa, almost every year since 2011. These events have major environmental, health, and economic impacts in the affected countries. Once on the shore, Sargassum is mechanically harvested and disposed of in landfills. Existing commercial applications of other brown algae indicate that the pelagic Sargassum could constitute a valuable feedstock for potential valorisation. However, limited data on the composition of this Sargassum biomass was available to inform on possible application through pyrolysis or enzymatic fractionation of this feedstock. To fill this gap, we conducted a detailed comparative biochemical and elemental analysis of three pelagic Sargassum morphotypes identified so far as forming Atlantic blooms: Sargassum natans I (SnI), S. fluitans III (Sf), and S. natans VIII (SnVIII). Our results showed that SnVIII accumulated a lower quantity of metals and metalloids compared to SnI and Sf, but it contained higher amounts of phenolics and non-cellulosic polysaccharides. SnVIII also had more of the carbon storage compound mannitol. No differences in the content and composition of the cell wall polysaccharide alginate were identified among the three morphotypes. In addition, enzymatic saccharification of SnI produced more sugars compared to SnVIII and Sf. Due to high content of arsenic, the use of pelagic Sargassum is not recommended for nutritional purposes. In addition, low yields of alginate extracted from this biomass, compared with brown algae used for industrial production, limit its use as viable source of commercial alginates. Further work is needed to establish routes for future valorisation of pelagic Sargassum biomass.

Rearrangement of the Cellulose-Enriched Cell Wall in Flax Phloem Fibers over the Course of the Gravitropic Reaction

The plant cell wall is a complex structure consisting of a polysaccharide network. The rearrangements of the cell wall during the various physiological reactions of plants, however, are still not fully characterized. Profound changes in cell wall organization are detected by microscopy in the phloem fibers of flax (Linum usitatissimum) during the restoration of the vertical position of the inclined stems. To characterize the underlying biochemical and structural changes in the major cell wall polysaccharides, we compared the fiber cell walls of non-inclined and gravistimulated plants by focusing mainly on differences in non-cellulosic polysaccharides and the fine cellulose structure. Biochemical analysis revealed a slight increase in the content of pectins in the fiber cell walls of gravistimulated plants as well as an increase in accessibility for labeling non-cellulosic polysaccharides. The presence of galactosylated xyloglucan in the gelatinous cell wall layer of flax fibers was demonstrated, and its labeling was more pronounced in the gravistimulated plants. Using solid state NMR, an increase in the crystallinity of the cellulose in gravistimulated plants, along with a decrease in cellulose mobility, was demonstrated. Thus, gravistimulation may affect the rearrangement of the cell wall, which can enable restoration in a vertical position of the plant stem.

Waterlogging increases organic carbon decomposition in grassland soils

Drainage increases soil organic carbon (SOC) mineralization, but waterlogging will not necessarily slow the SOC decomposition. We investigated the effects of water regime (anaerobic vs. aerobic conditions) on SOC mineralization in adjacent wetland and grassland soils with contrasting biochemical properties in the east Tibetan Plateau. Biochemical characteristics of SOC (compositions of non-cellulosic and amino polysaccharides) were analyzed, then soils were moistened to 70% of water-holding capacity (aerobic) or saturated (anaerobic) and incubated at 25 °C for 95 days. The non-cellulosic sugar content in SOC was similar in wetland and meadow soils, but the mass ratio of hexoses (galactose plus mannose or rhamnose plus fucose) to pentoses (arabinose plus xylose) was three-fold greater in meadows than wetlands. Furthermore, there was three times more accumulation of microbial residues (sum of glucosamine, mannosamine, galactosamine, and muramic acid) in SOC, and more fungal than bacterial necromass in the meadow than wetland soils. This suggests slower transformation of plant litter by microorganisms, and thus less accumulation of microbial products in the SOC of wetlands than meadows. Meadow soils had more microbial biomass and higher activities of β–glucosidase, phenol oxidase, and urease per unit of SOC than wetland soils. During the 95-day incubation, C loss (as CO2) per unit of SOC was 55–73% greater in anaerobic than aerobic meadow soils, but was 59–64% less from wetland soils under anaerobic conditions than aerobic conditions. Thus, SOC decomposition in anaerobic conditions was dependent on the degree of SOC degradation, which was related to the initial proportion of plant and microbial residues. We addressed the importance of biochemical nature in regulating SOC decomposition under anaerobic conditions. We conclude that both the wetland drainage and grassland waterlogging increase SOC mineralization (measured as CO2 evolution) in alpine areas.

Management of grasslands by mowing versus grazing – impacts on soil organic matter quality and microbial functioning

Although 30% of the European surface area is covered with grasslands, little is known about the effect of their management on soil quality and biogeochemical cycling. Here, we analysed soil from an experimental site in Western France, which had been under either grazing or mowing regime for 13 years. We aimed to assess the effect of the two management practices on the biogeochemical functioning of the soil system. To this end we compared soil organic matter (SOM) composition and microbial properties at two depths. We analysed for elemental, lignin and non-cellulosic polysaccharide content and composition, microbial biomass, soil microbial respiration and enzyme activities. Our results showed higher soil organic carbon (SOC) and nitrogen contents in the surface soil under grazing as compared to mowing. Soil biogeochemical properties also differed between grazing and mowing treatments. In particular, soil under grazing showed lower lignin and higher microbial biomass. Despite the similar non-cellulosic polysaccharide content under both treatments, microbial community under mowing was characterised by higher enzyme production per microbial biomass, leading to more degraded SOM in the mowing system as compared to grazing. We conclude that grazing and mowing regimes impact differently biogeochemical soil functioning. Higher and more diverse carbon input under grazing compared to mowing may lead to enhanced substrate availability and thus more efficient microbial functioning, which could favour SOC sequestration through formation of microbial products.

Thermochemical conversion of apple seeds before and after supercritical CO2 extraction: an assessment through evolved gas analysis

In the food industry, apple seeds are usually considered as a waste despite their potential to be further exploited in a biorefinery perspective. In this study, apple seeds before and after supercritical CO2 extraction were thoroughly characterized to mark the effects of the extraction process on their physicochemical characteristics and unveil their hidden value. Moreover, thermal degradation of apple seeds was carried out by means of a thermogravimetric analyzer coupled with a Fourier transform infrared spectrometer for evolved gas analysis (TG/EGA) to assess their energetic potential. The results showed that the main compound extracted was the lipid fraction. The exhausted biomass still contained cellulosic and noncellulosic polysaccharides as well as proteins that could be further valorized. Thermogravimetry combined with the analysis of evolved gas showed some characteristic temperatures that could be attributed to the degradation of different biological compounds.

Response of the Organic Carbon Fractions and Stability of Soil to Alpine Marsh Degradation in Zoige, East Qinghai-Tibet Plateau

Soil organic carbon (SOC) fractions play a crucial role in the carbon cycle of alpine wetland ecosystems and regional climate regulation. In the last decades, alpine marshes at Tibetan Plateau have undergone increased degradation. The effects of marsh degradation on soil labile and recalcitrant organic carbon fractions and on SOC stability remain unclear. In this study, we investigated changes in SOC fractions with different biochemical recalcitrance and stability in the marsh ecosystems with various levels of degradation in the Zoige Wetland, Eastern Qinghai-Tibet Plateau. Soil samples of relatively pristine marsh (RPM), lightly degraded marsh (LDM), moderately degraded marsh (MDM), heavily degraded marsh (HDM) and severely degraded marsh (SDM) were collected and analysed using the acid hydrolysis method. Compared with RPM, the concentrations of SOC labile fraction I (non-cellulosic polysaccharides), SOC labile fraction II (cellulose) and recalcitrant organic carbon (ROC) in degraded marshes decreased significantly: 29–97, 38–95 and 32–99%, respectively, except for the LDM. The SOC recalcitrance index (RIc) in the HDM and SDM was significantly lower than that in RPM by 11–35 and 15–47%, respectively; compared with RPM, the stability of organic carbon was significantly lower in the HDM and SDM. Additionally, the marsh degradation increased vertical variation in the concentrations of SOC fractions. The degradation of alpine marshes decreases the capacity for carbon (C) sequestration via decreasing the resistance of SOC to biodegradation, indicating that the alpine marsh degradation may weaken the carbon sink capacity of the Zoige Plateau, thus aggravating the global greenhouse effect.

Cellulose Nanofibrils as Assay Substrates for Cellulases and Lytic Polysaccharide Monooxygenases

A facile method for preparing colloidal cellulose nanofibrils (CNFs) from potato tubers is presented comprising alkaline treatment to remove noncellulosic polysaccharides, followed by hypochlorite ...