Organic pollutants are widely distributed in the environment. Due to their physical and chemical characteristics, they tend to be biomagnified in food chains, mainly in aquatic organisms. Therefore, food consumption is a significant route of lifelong exposure. Although the Amazon River basin contains the highest freshwater biodiversity on Earth, there is scarce literature focusing on the levels of organic pollutants in the local population. The present study was aimed at assessing the levels of several environmental pollutants (polycyclic aromatic hydrocarbons, bisphenols, parabens, and benzophenones) in urine samples from riverside communities along the Tapajós and Amazon Rivers in the Brazilian Amazon region. The results show a 100 % detection of naphthalene metabolites (namely, 1-hydroxy-naphthalene (1OH-NAP), 2-hydroxy-naphthalene (2OH-NAP)), with the highest levels belonging to benzylparaben (BzP) (17.3 ng/mL). Gender-specific analysis revealed that women had significantly higher levels of certain PAH metabolites (i.e., 1OH-NAP and 2-hydroxy-fluorene (2OH-FLU)) than men. In turn, most of the evaluated compounds were higher in urine samples from people living near the Amazon River, which presents increased traffic of boats and ships than the Tapajós River. On the other hand, the human health risk assessment suggested that all communities are at risk of suffering non-carcinogenic effects from exposure to PAHs. At the same time, they are also at risk of carcinogenic effects from exposure to benzo[a]pyrene metabolites. Thus, further studies are needed in order to evaluate the potential health effects of exposure to a mixture of these organic pollutants and other contaminants present in the area, such as mercury.