Non-anthocyanin Polyphenols Research Articles

Study of the mass spectrometric behaviors of anthocyanins in negative ionization mode and its applications for characterization of anthocyanins and non‐anthocyanin polyphenols

Traditionally, anthocyanin analysis in mass spectrometry is carried out in the positive ionization mode only. A study of the mass spectrometric behaviors of anthocyanins in the negative ionization mode revealed interesting characteristics that was not previously reported. It can be used to differentiate anthocyanins from other non-anthocyanin polyphenols. An ultra-high-performance liquid chromatography with high-resolution mass spectrometry (U-HPLC/HRMS) method was developed. The method used multiple-stage mass fragmentation in both the negative and positive ion modes. The whole cycle time of the new method is 1.8 s for two full scans and six data-dependent scans. The new strategy found, in the negative ionization mode, a series of characteristic ions, e.g. [M-2H](-), [M-2H + H(2)O](-), formic acid adducts, and doubly charged ions were observed for the MS analysis of anthocyanins. The characteristic ions can be used for identification and differentiation of anthocyanins and non-anthocyanin phenolic compounds. Comprehensive studies were performed on the differentiation of anthocyanins and non-anthocyanin polyphenols in blueberry (Vaccinium cyanococcus), Hongcaitai (Brassica compestris L. var. purpurea Bailey), and red radish (Raphanus sativus var. longipinnatus 'Shinrimei'). The data generated from a single LC run enables rapid and reliable differentiation and identification of anthocyanins and non-anthocyanins in botanicals and foods. Published 2012. This article is a US Government work and is in the public domain in the USA.

Liquid chromatography/mass spectrometry based fingerprinting analysis and mass profiling of Euterpe oleracea (açaí) dietary supplement raw materials

Chemical fingerprinting and mass profiling methods to identify biologically active compounds in botanical dietary supplements is gaining much attention in recent years. Euterpe oleracea (açaí) has been reported to be rich in health-beneficial chemical constituents. We have developed LC/MS based fingerprinting and mass profiling methods to identify fatty acids, anthocyanins and non-anthocyanin polyphenols in three processed raw materials; non-organic açaí powder (ADSR-1), raw-organic açaí powder (ADSR-2) and freeze-dried açaí powder (ADSR-3) that are used in the preparation of botanical dietary supplements. For LC/MS analysis of fatty acids and non-anthocyanin polyphenols, the açaí samples were extracted sequentially with dichloromethane followed by methanol. To study fingerprinting analysis of anthocyanins, açaí samples were extracted with acidic methanol-water. The LC separation of fatty acids, non-anthocyanin polyphenols and anthocyanins in açaí raw materials was achieved using a C18 column with a gradient mobile phase consisting of solvents A (0.1% formic acid in water), and B (0.1% formic acid in methanol). MS experiments were carried out with negative and positive mode electrospray ionization. LC/MS analysis of dichloromethane extracts of (ADSR-1), (ADSR-2) and (ADSR-3) açaí powders have shown to contain fatty acids, γ-linolenic acid, linoleic acid, palmitic acid, and oleic acid. Whereas, the fingerprinting analysis of methanol extracts of ADSR-1, ADSR-2 and ADSR-3 led to the identification of phenolic acids, anthocyanin and non-anthocyanin polyphenols. The results from our study may be useful for the authentication and quality assessment of açaí dietary supplement raw materials.