Residual cavity mode noise and weak output signal are the main factors that restrict the detection performance of off-axis integrated cavity output spectroscopy (OA-ICOS). In order to overcome the above drawbacks, a novel V-shaped off-axis integrated cavity output spectroscopy (V-OA-ICOS) device was demonstrated, and the dual-path coupling method was further proposed. The dual-path coupling V-OA-ICOS device can not only suppress the residual cavity mode noise but also enhance the intensity of the output signal. The V-shaped resonator cavity ensures a more uniform and square-shaped spot pattern distribution on the surface of the highly reflective mirrors, significantly reducing spots overlap. The dual-path coupling method can re-couple the laser reflected by the front cavity mirror, thereby increasing the total energy and mode density of the cavity. The spectrum of water vapor stable isotopes HD16O and H216O at 3.66 μm was measured to illustrate the performance of the presented approach, the dual-path coupling V-OA-ICOS increases the signal-to-noise ratio (SNR) by a factor ∼ 2.1 compared to single path V-OA-ICOS and the signal intensity by a factor of ∼ 2. The δD variation was obtained by measuring the outdoor water vapor isotope using this device for three consecutive days. The variation range of δD is −220‰ ∼ −120‰. This work proposes a new cavity structure and optical re-coupling method, which offers a novel approach to enhance the performance of OA-ICOS devices.