Background: Sentinel lymph node biopsy (SLNB) using radio-pharmaceutical and a blue dye is gold standard for axillary staging in clinically node-negative early breast cancer and increasingly being used for post NACT cN0 axilla as well. High costs and limited availability of radio-pharmaceutical and/or gamma probe are major deterrents in performing SLNB in developing countries. In this study, we evaluated feasibility of SLN identification (SLN-IR) of fluorescein-guided (FG) SLNB in combination with methylene blue dye (MBD). Methods: This was a prospective cross-sectional non-randomized validation study in patients with post NACT clinically node negative axilla. Patients underwent validation SLNB using fluorescein (and blue LED light) and MBD. Axillary dissection was performed irrespective of SLNB histology. SLIN-IR and False Negative Rate (FNR) were assessed. Results: The SLNs were identified in 51 out of 56 (91%) post Neoadjuvant Chemotherapy (NACT) patients. The median number of sentinel lymph nodes identified 1 (range 1–3) in post NACT patients. The SLN-IR using MBD was 91%, FD was 85%, and combined MBD FD was 89%. The false negative rate (FNR) was 7.8% (MBD), 8.3% (FD) and 7.8% (MBD+FD) Conclusions: This prospective validation study showed adequate SLN-IR and FNR using low cost dual dyes in post NACT cN0 patients and can be used in low resource settings. No conflict of interest.