Nitrogen Removal In Wastewater Treatment Research Articles

Insight into performance of nitrogen removal enhanced by adding lactic acid-rich food waste fermentation liquid as carbon source in municipal wastewater treatment

Lactic acid-rich fermentation liquid (LAFL) of food waste is found to act as a promising alternative carbon source for nitrogen removal in wastewater treatment. Here, LAFL was employed to investigate its impacts on nitrogen removal during raw municipal wastewater treatment with a comparison to sodium acetate (NaAc). Results indicated that nitrogen removals were comparable when incorporated with LAFL and NaAc (92.89 % v.s. 91.23 %). Unlike the utilization of NaAc, using LAFL could avoid suppressing the relative abundance of the nitrification genes and thus pose a negative risk to nitrogen removal during prolonged operation. The introduction of LAFL increased the stability and robustness of the functional microbial community and effectively reduced excess activated sludge (AS) generation by 109 % compared to NaAc addition, consequently enhancing nitrogen removal but diminishing the treatment cost. In general, LAFL exhibits prospective engineering application potentials and economic advantages in improving nitrogen removal by AS process.

High-efficient nitrogen removal with low demand of Fe source and mechanism analysis driven by Fe(II)/Fe(III) cycle

Feammox (Fe(III) reduction coupled with anaerobic ammonium oxidation) is less studied for nitrogen removal in wastewater treatment, and the limited studies show its low removal efficiency and need for continuous resupply of Fe(III) sources. In this study, sustainable and efficient nitrogen removal was obtained by the Fe redox cycle with only 1 g/L Fe2O3 dosage. The removal efficiencies of NH4+-N and TN were 100% and 92.45%, respectively, significantly higher than previous studies (∼50%). Fe(III) was reduced to Fe(II) by Feammox and subsequently regenerated by NDFO (NOx--N-dependent Fe(II) oxidation) and chemical oxidization. NOx--N produced by Feammox was also reduced to N2 by NDFO. The iron-reducing bacteria (Ferruginibacter and Dechloromonas) and the iron-oxidizing bacteria (Rhodanobacter and Thermomonas) were enriched by the addition of Fe2O3 and played important roles in Feammox and NDFO, respectively. Results of X-ray diffraction showed that Fe(OH)2 was produced from Feammox and then transformed to lepidocrocite by Fe(II) oxidation. Metagenomic analysis showed D/ANRA (dissimilatory/ assimilatory nitrate reduction to ammonia) were the potential nitrogen conversion pathways. N2O as an intermediate product of Feammox or NDFO was undetected. The genes associated with Fe transport were upregulated, accelerating the transfer of electrons between microorganisms and Fe(Hydr)oxide. The process for nitrogen removal by the Fe redox cycle requires no aeration and no organic carbon source, and can reduce the need for Fe(III) sources. It offers a promising method for nitrogen removal from wastewater.

Bibliometric Analysis of Research Trends Related to Carbon Sources for Nitrogen Removal in Wastewater Treatment

The low organic carbon content of municipal wastewater reduces the effectiveness of biological nitrogen removal. Herein, we reviewed recent trends in carbon sources for nitrogen removal during wastewater treatment. A bibliometric analysis using the Science Citation Index Expanded database from the Web of Science was performed to analyze articles published in this field from 2000 to 2022. The major conclusions are as follows: (1) publications from China ranked 1st, followed by those from the USA and India during the statistical period. Regarding research institutions, the Chinese Academy of Sciences was the largest contributor with the most publications. (2) Based on the trend analysis of keywords, further research hotspots were discussed, showing related research on external carbon sources. Research on traditional carbon sources (e.g., methanol and ethanol) has decreased, whereas that on alternative carbon sources, such as sludge hydrolytic acidification liquid, fermentation liquid of food waste, agricultural waste, and biodegradable polymers, has increased. (3) New nitrogen removal technologies, particularly anammox, are becoming increasingly popular. (4) In the study on nitrogen removal, increasing attention is paid to changes in the microbial community, and the biological mechanism of nitrogen removal is discussed from a microscopic perspective.

Achieving superior nitrogen removal in an air-lifting internal circulating reactor for municipal wastewater treatment: Performance, kinetic analysis, and microbial pathways

Anticipated growth in living standards has accentuated higher requirements for effluent quality from municipal wastewater treatment. In this study, an air-lifting internal circulating reactor with a high internal circulation ratio (36:1) was established to treat municipal wastewater with a long-term operation. In the bioreactor, the average effluent chemical oxygen demand, total nitrogen, and ammonium nitrogen could be 13.1, 5.7, and lower than 1 mg/L, respectively. Further analysis of nitrogen removal showed that traditional nitrification and denitrification, simultaneous nitrification and denitrification (SND), and nitrogen assimilation accounted for 27.4 %, 68.7 %, and 3.9 % respectively. The proportion of aerobic bacteria (Saprospiraceae) and facultative bacteria (Comamonadaceae) were significantly increased, indicating a higher capacity for organic degradation in the reactor. The relative abundance of denitrifying bacteria and bacterial groups with SND (Comamonadaceae) increased. These results suggested the air-lifting internal circulating reactor could be a viable and efficient option for superior nitrogen removal in wastewater treatment.

Fe-C wrapped in polyurethane sponge cubes tied in anoxic zones to enrich multiple denitrifying bacteria enhancing wastewater nitrogen removal

To enhance nitrogen removal in wastewater treatment, a novel method of wrapping waste iron shavings (Fe-C) in polyurethane sponge cubes tied in anoxic zones of a continuous reactor was proposed. Three types of denitrifying bacteria were enriched simultaneously in biofilms and activated sludge, including iron autotrophic denitrifying bacteria (7.83–8.71 %), heterotrophic denitrifying bacteria (10.60–11.95 %), and heterotrophic nitrification-aerobic denitrifying bacteria (23.47–40.06 %). Iron autotrophic denitrifying bacteria (Dechloromonas and Sphaerotilus) can remove NO3−-N using Fe2+ as electron donor produced by micro-electrolysis of iron shavings without organic carbon demand, and heterotrophic nitrification-aerobic denitrifying bacteria (Ferribacterium and Zoogloea) can simultaneously accomplish nitrification and denitrification under aerobic conditions. The enrichment of these two functional bacteria strengthened the nitrogen removal performance compared to conventional heterotrophic denitrifying process, which could achieve efficient and stable total nitrogen removal (87.4 %) even with low organic carbon. In addition, the synergy of biological and chemical processes through Fe-C micro-electrolysis also enhanced total phosphorus removal (86.0 %).

Controlling anammox speciation and biofilm attachment strategy using N-biotransformation intermediates and organic carbon levels

Conventional nitrogen removal in wastewater treatment requires a high oxygen and energy input. Anaerobic ammonium oxidation (anammox), the single-step conversion of ammonium and nitrite to nitrogen gas, is a more energy and cost effective alternative applied extensively to sidestream wastewater treatment. It would also be a mainstream treatment option if species diversity and physiology were better understood. Anammox bacteria were enriched up to 80%, 90% and 50% relative abundance, from a single inoculum, under standard enrichment conditions with either stepwise-nitrite and ammonia concentration increases (R1), nitric oxide supplementation (R2), or complex organic carbon from mainstream wastewater (R3), respectively. Candidatus Brocadia caroliniensis predominated in all reactors, but a shift towards Ca. Brocadia sinica occurred at ammonium and nitrite concentrations > 270 mg NH4–N L−1 and 340 mg NO2–N L−1 respectively. With NO present, heterotrophic growth was inhibited, and Ca. Jettenia coexisted with Ca. B. caroliniensis before diminishing as nitrite increased to 160 mg NO2–N L−1. Organic carbon supplementation led to the emergence of heterotrophic communities that coevolved with Ca. B. caroliniensis. Ca. B. caroliniensis and Ca. Jettenia preferentially formed biofilms on surfaces, whereas Ca. Brocadia sinica formed granules in suspension. Our results indicate that multiple anammox bacteria species co-exist and occupy sub-niches in anammox reactors, and that the dominant population can be reversibly shifted by, for example, changing nitrogen load (i.e. high nitrite concentration favors Ca. Brocadia caroliniensis). Speciation has implications for wastewater process design, where the optimum cell immobilization strategy (i.e. carriers vs granules) depends on which species dominates.

Start-up characteristics and microbial nitrogen removal mechanisms in ANAMMOX systems with different inoculations under prolonged starvation

The characteristics of lab-scale ANAMMOX system for efficient nitrogen removal were tested by different inoculations (anaerobic granular sludge (AGS) + anammox sludge (AMS), R1; AGS alone, R2) in continuous flow reactors following start-up under starvation. TN removal rates were above 90 % in both reactors. Microbial community analysis showed that the nitrogen removal was the combined results of anammox and partial denitrification (PD), Higher microbial growth rate triggered by quorum-sensing (QS) caused R1 to secrete more extracellular polymeric substances (EPS) and promoted the growth of its particle size. However, the overproduction of EPS destroyed the particle structure with time, and led to a deterioration of operational stability in R1. R2 showed better nitrogen removal stability to high nitrogen loading rate (NLR). This was due to higher endogenous organics within R2, which inhibited cell aggregation and EPS overproduction. But undeniably, R1 had higher specific anammox activity (SAA) with higher anammox bacteria (AAOB) abundance (The abundance of Planctomycetota in R1 and R2 were 21.2 %, 7.71 %). The results suggest that the operational stability was influenced by a combination of particle properties and microbial metabolism, and that the inoculation of AGS is more conducive to efficient and economic nitrogen removal in wastewater treatment under starvation.

Effects of Heavy Metals on the Performance and Mechanism of Anaerobic Ammonium Oxidation for Treating Wastewater

Persistence of ammonium nitrogen and heavy metals in wastewater still remains a challenge, and many wastewater treatment plants face the challenge of removing nitrogen under heavy metal stresses. There is no preferred method for the biological treatment of wastewater containing nitrogen and heavy metals with the possible exception of the anaerobic ammonium oxidation (anammox), since it has shown promise for removing nitrogen under heavy metal stresses. This article reviews the recent research results of the nitrogen-removal performance and mechanism by the anammox process under heavy metal stresses, mainly discussing the enhancing and inhibition effects of heavy metals on the performance of the Anammox reactor. The influencing mechanism of heavy metals on the microbial community and extracellular polymeric substances is also presented, and examples are given for explanation. The main problems of the present research are pointed out, and it is proposed that unifying the metal ion concentrations of inhibiting or promoting anammox activity is necessary for the development and industrial application of the anammox process. The information of this review can offer a great possibility for achieving desired nitrogen removal in wastewater treatment under heavy metal stresses and with significant energy savings.

Disturbance-based management of ecosystem services and disservices in partial nitritation-anammox biofilms.

The resistance and resilience provided by functional redundancy, a common feature of microbial communities, is not always advantageous. An example is nitrite oxidation in partial nitritation-anammox (PNA) reactors designed for nitrogen removal in wastewater treatment, where suppression of nitrite oxidizers like Nitrospira is sought. In these ecosystems, biofilms provide microhabitats with oxygen gradients, allowing the coexistence of aerobic and anaerobic bacteria. We designed a disturbance experiment where PNA biofilms, treating water from a high-rate activated sludge process, were constantly or intermittently exposed to anaerobic sidestream wastewater, which has been proposed to inhibit nitrite oxidizers. With increasing sidestream exposure we observed decreased abundance, alpha-diversity, functional versatility, and hence functional redundancy, among Nitrospira in the PNA biofilms, while the opposite patterns were observed for anammox bacteria within Brocadia. At the same time, species turnover was observed for aerobic ammonia-oxidizing Nitrosomonas populations. The different exposure regimens were associated with metagenomic assembled genomes of Nitrosomonas, Nitrospira, and Brocadia, encoding genes related to N-cycling, substrate usage, and osmotic stress response, possibly explaining the three different patterns by niche differentiation. These findings imply that disturbances can be used to manage the functional redundancy of biofilm microbiomes in a desirable direction, which should be considered when designing operational strategies for wastewater treatment.

Fractional order-based hierarchical controller design and evaluation with Bürger-Diehl settler model in a total nitrogen removal wastewater treatment process.

The primary objective of this study was to establish two-level structured control techniques based on the globally known benchmark simulation model no. 1 (BSM1) and the Bürger-Diehl settler model in order to improve effluent quality. The latter was based on the activated sludge model no. 1 (ASM1), while the classic Takacs model was superseded by the more recent Bürger-Diehl settler model with enhanced predictive potential. A two-level hierarchical control structure was considered to maintain the dissolved oxygen concentration basing on the ammonia levels and also a nitrate controller was considered to improve nitrogen removal efficiency. Fractional order PI controllers were considered at the secondary level and advanced control techniques, namely, MPC and fuzzy, were implemented at the primary level. Two advance control schemes, being, FPI-MPC and FPI-fuzzy were designed in the present work. The controllers were designed based on the plant model which was identified using prediction-error minimization method. It was observed that the implemented control strategies in consideration with the plant modifications showed a profound impact in improving the plant performance in terms of the effluent quality. FPI-fuzzy resulted in noticeable 60% and 53% reduction in total nitrogen violations for dry and storm climatic conditions, respectively.

Test of Membrane Aerated Biofilm Reactors for Nitrogen Removal in Wastewater Treatment

Membrane aerated biofilm reactor, as a biological wastewater treatment technology, has been nearly mature on a commercial scale. It uses bubble-free aeration to provide oxygen for biological nitrification and wastewater degradation. A novel oxygen-permeable hollow fiber membrane (Zeelung cord) specifically designed for use in a membrane aerated biofilm reactors (MABR). These fibers are organized into bundles, which are wrapped around the reinforcing core to increase strength. This permeable membrane allows oxygen to diffuse into the attached biofilm, which directly leads to the biological oxidation of pollutants in the wastewater. This study aimed to determine the nitrification and oxygen transfer capacity of Zeelung fibers used in the MABR system. The effects of various C/N ratios (in the range of 1.0 to 3.0) on the membrane modules were studied using three laboratory-scale reactors over the course of 165 days. In this test, the average removal efficiency of COD can reach 74% under selected conditions, up to 90%. Meanwhile, the average nitrification rate is 3.9 g/d/m2, the average ammonia removal rate is 90%, and the maximum value can reach 99%. In addition, the oxygen transfer rate of the fiber in the liquid phase was 19.65 g/d/m2. The experiment also indicated that the nitrification rate is directly proportional to the transfer flux of oxygen and is related to the content of dissolved oxygen in the water. The nitrification rate can be controlled by controlling the concentration of dissolved oxygen in water, thus affecting the removal rate of ammonia nitrogen.

Impact of Nitrogen Removal in Wastewater Treatment on NDMA Formation at Downstream Drinking-Water Treatment Plants

AbstractDe facto reuse (DFR) occurs when surface waters receiving wastewater effluent are used as source water by a downstream drinking-water treatment plant (DWTP). Wastewater effluent may contain...

An Experimental Study on the Biological Fixation and Effective Use of Carbon Using Biogas and Bacterial Community Dominated by Methanotrophs, Methanol-Oxidizing Bacteria, and Ammonia-Oxidizing Bacteria

This study used biogas from a wastewater treatment plant and bacterial community where methanotrophs, Methylophilus, and ammonia-oxidizing bacteria clusters coexisted to propose an effective method for biological carbon fixation and nitrogen removal in wastewater treatment for carbon capture, utilization, and storage (CCUS). Biogas from wastewater treatment plant was provided, instead of purified CH4, to provide operation and maintenance conditions of bio-catalyst reaction for efficient biological carbon fixation by bacterial community using methane and CO2. This study assessed the conditions to induce type X methanotrophs that can use CO2 as a carbon source, as dominant species in the bacterial community and continuously and effectively supply reducing equivalents required for the conversion of CO2 to methanol within the system. Herein, the results of inducing efficient co-existence of methanotrophs, Methylophilus, and ammonia-oxidizing bacteria cluster in the bacterial community were shown.

Inhibitory effect of oil and fat on denitrification using food waste fermentation liquid as carbon source

Food waste fermentation liquid (FWFL) can be used as carbon source to enhance nitrogen removal in wastewater treatment. However, the influence of lipid, a common component of food waste, on denitrification remains unclear. In this study, the effect of oil and fat on denitrification process and the underlying mechanisms were investigated using synthetic oil- and fat-bearing carbon source and verified with real FWFL. In the batch experiment, oil and fat had no obvious influence on denitrification, but in the semi-continuous experiment, the denitrification rate in the oil- and fat-added assays decreased to 44% and 38% of that in the control, respectively, after 45 batches. Oil and fat caused sludge floatation, and the floating sludge thickness increased with the continuous operation. Oil/fat-sludge aggregates were observed in the floating sludge and limited gas release. Microbial community analysis indicated that oil and fat did not affect denitrifying bacteria abundance. Limitation of mass transfer might be the main reason for the inhibition of oil and fat on denitrification. In the real FWFL experiment, the denitrification rate in the original and emulsified oil-bearing FWFL decreased to 24% and 56% of that in the demulsifying FWFL, respectively, after 45 batches. These findings indicate the necessity of removing lipids when FWFL is used as denitrification carbon source.

Potential of anammox process towards high-efficient nitrogen removal in wastewater treatment: Theoretical analysis and practical case with a SBR

Anammox process has been widely applied in the wastewater nitrogen removal for its high rate and low cost. However, few researches reported the process potential in treating low-strength nitrogen wastewater. In this study, a sequencing batch reactor (SBR) was taken to explore the feasibility of low-strength nitrogen wastewater treatment by anammox process in theory and practice. The practical operation indicated that the effluent with satisfactory quality (1.90 ± 0.70 mg-TN·L−1) could be achieved, when the SBR was fed with low-strength nitrogen influent (6.20 ± 0.45 mg-NH4+-N·L−1 and 7.96 ± 0.59 mg-NO2--N·L−1). The hydraulic retention time (HRT), nitrogen removal efficiency, nitrogen removal rate (NRR) and hydraulic loading rate of SBR were 5.42 h, 86.5%, 0.054 kg-N·m−3·d−1 and 4.43 m3·m−3·d−1 during the 79-day operation, respectively. The theoretical analysis revealed the potential of anammox SBR. When SBR is stably operated, the maximum NRR would be 0.062 kg-N·m−3·d−1 if the effluent nitrogen was required to be as low as 3 mg·L−1. The NRR value is feasible for engineering. However, considering the lower specific substrates utilization rate in practice, the maximum stable NRR was calibrated and found inefficient afterwards. In order to improve the potential of anammox process, the reactors without back mixing and with periodic bioaugmentation should be taken in priority for the engineering applications. In particular, the bioaugmentation frequency and single addition amount were calculated as 7 d and 0.3 g-VSS·L−1, respectively. The results may provide guidance for the development of high-efficient and stable nitrogen removal process under low-strength condition.

Intensification of activated sludge sedimentation processes by means of vacuuming

Introduction. The mixed liquor of nitrogen removal wastewater treatment plants is characterized by a high concentration of nitrates and dissolved oxygen at the inlet to the secondary settling tank. In the sludge layer of secondary sedimentation tanks, conditions of decreased oxygen content and uncontrolled denitrification processes take place. This leads to the floating up and removal of sludge with the effluent and secondary pollution of treated water. The purpose of this article is to study the parameters of activated sludge sedimentation in municipal wastewater treatment plants and their intensification by means of vacuuming.
 
 Materials and methods. The studies were carried out under laboratory conditions. Activated sludge vacuuming and sedimentation processes were simulated. Diagrams of the “sludge-water” phase reduction (Kinsh curves) were drawn. Mathematical and graphic processing of the results was carried out.
 
 Results. Biological treatment of municipal wastewater (aerotank — secondary settling tank) and methods of its intensification by influencing the activated sludge were considered in this article. Trends of activated sludge (at different concentrations of mixed liquor suspended solids) sedimentation were experimentally obtained for municipal wastewater treatment plants. The process of sludge vacuuming was researched, the process efficiency was determined as a function of the treatment time.
 
 Conclusions. Vacuuming allows removing gases from the fluid, which accelerates the process of sludge separation from the treated water and prevents it from floating to the surface. The treated sample is characterized by better sedimentation characteristics, density, coarseness and integrity of flakes. The optimal duration of mixed liquor vacuuming before sedimentation is 0.5 minutes; this accelerates the processes of subsequent sedimentation and reduces the removal of sludge with treated water. The results of laboratory tests can be applied to the design of the mixed liquor vacuuming unit before the secondary sedimentation tanks and its sludge separation.

Transcriptomics Uncovers the Response of Anammox Bacteria to Dissolved Oxygen Inhibition and the Subsequent Recovery Mechanism.

Understanding the recovery of anaerobic ammonium-oxidizing (anammox) bacteria after inhibition by dissolved oxygen (DO) is critical for the successful applications of anammox-based processes. Therefore, the effects of oxygen exposure (2 mg L-1 DO for 90 min) and subsequent recovery treatments [N2 purging or nano zero-valent iron (nZVI) addition] on the activity and gene expression in a Kuenenia stuttgartiensis enrichment culture were examined. Combining the self-organizing map clustering and enrichment analysis, we proposed the oxidative stress response of anammox bacteria based on the existing concepts of oxidative stress in microbes: the DO exposure triggered a stringent response in K. stuttgartiensis, which downregulated the transcription levels of genes involved in the central metabolism and diverted energy to a flagellar assembly and metal transport modules; these changes possibly promoted survival during the inhibition of anammox activity. According to the cotranscription with central catabolism genes, putative reactive oxygen species (ROS) scavenger genes (kat and sod) were presumed to detoxify the anammox intermediates rather than ROS. In addition, both activity and mRNA profiles with appropriate amount of nZVI addition (5 and 25 mg L-1) were close to that of control, which proved the effectiveness of nZVI addition in anammox recovery. These results would be relevant to the physio-biochemistry development of anammox bacteria and further enhancement of nitrogen removal in wastewater treatment.

Trends of microbial electrochemical technologies for nitrogen removal in wastewater treatment

Trends of microbial electrochemical technologies for nitrogen removal in wastewater treatment

The community compositions of three nitrogen removal wastewater treatment plants of different configurations in Victoria, Australia, over a 12-month operational period.

Amplicon sequence fingerprinting of communities in activated sludge systems have provided data revealing the true level of their microbial biodiversity and led to suggestions of which intrinsic and extrinsic parameters might affect the dynamics of community assemblage. Most studies have been performed in China and Denmark, and comparatively little information is available for plants in other countries. This study looked at how the communities of three plants in Victoria, Australia, treating domestic sewage changed with season. All were designed to remove nitrogen microbiologically. They were all located close together to minimise any influence that climate and demographics might have on their operation, and samples were taken at weekly intervals for 12 months. 16S rRNA amplicon sequencing revealed that each plant community was distinctively different to the others and changed over the 12-month sampling period. Many of the factors suggested in other similar studies to be important in determining community composition in activated sludge systems could not explain the changes noted here. The most likely influential factors were considered to be temperature and influent composition reflecting changes in dietary intake by the populations served by each plant, since in all three, the most noticeable changes corresponded to seasonal shifts. KEY POINTS: • Monitoring microbial communities in 3 wastewater treatment plants removing nitrogen • Temperature is the most influential factor in dynamic changes in community composition.

Optimization for the Operational Parameters of the Partial Nitrification in a Fluidized Bed Bioreactor (PNFBR)

Partial nitrification combined with anaerobic ammonium oxidation (Anammox) process has been recognized as a promising technology for the nitrogen removal in wastewater treatment. This research aimed to focus on how to successfully achieve the partial nitrification in a fluidized bed bioreactor (PNFBR) for the treatment of synthetic wastewater with high ammonia concentration. The influence factors, including dissolved oxygen (DO) levels, free ammonia (FA)/free nitrous acid (FNA) concentration, ambient temperature, influent ammonia concentration, and alkalinity, were investigated to achieve the goal of high nitrite accumulation. Results exhibit that the optimal conditions for the PNFBR was 30 °C with the 7:1 of ratio of alkalinity to influent nitrogen, 1 mg/L of DO concentration, 28 day of SRT, 16.6 day of hydraulic retention time (HRT), and 400 mg/L of influent ammonia concentration. Additionally, at the lower temperature (20 °C), high nitrite accumulation was also attained by increasing the alkalinity (8:1 of ratio of alkalinity to influent nitrogen) in system. Compared with other biofilm system, the PNFBR with the new media mixture of acrylonitrile butadiene styrene (ABS) plastic and its modified one could achieve the effluent requirements for anammox, with the PNFBR effluent NO2--N:NH4+-N ratio appropriately at 1:1.