An experiment was conducted during Boro rice in the permanent experimental field of the Department of Soil Science, Bangladesh Agricultural University (BAU), Mymensingh to investigae the long term outcome of manure and fertilizers on the yield and Nitrogen Use Efficiency (NUE) of BRRI dhan29 in floodplain (Subtropical) soil (Aeric Haplaquepts). The experiment was designed in a randomized complete block design with three replications. There were ten treatment combinations viz, control; Nitrogen (N); (Nitrogen Phosphorus) NP; Nitrogen Potassium (NK); Nitrogen Phosphorus Potassium (NPK); Nitrogen Zinc (NZn); Nitrogen Sulphur (NS); Nitrogen Sulphur Zinc (NSZn), Nitrogen Phosphorus Potassium Sulphur Zinc (NPKSZn) and NPK+FYM. The yield contributing characters and yield of BRRI dhan29 significantly increased due to different treatments. Grain and straw yields were highly influenced by the application of nutrients in various combinations. The longest panicle length (25.3 cm), the highest 1000 grain weight (22.7 g), the maximum grain yield (6.29 t ha-1), the highest nitrogen uptake in grain (70.4 kg ha-1) were found for the NPKSZn treatment. Grain yield increased by 24.8%, 85.8%, 40.7%, 68.9%, 29.2%, 33.2%, 107.1%, 28.9% and 78.9% due to N, NP, NK, NPK, NS, NSZn, NPKSZn, NZn and NPK+FYM treatments respectively and the corresponding straw yields attainment by 49.6%, 51.1%, 82.0%, 95.3%, 37.8%, 34.4%, 82.7%, 41.8% and 90.2%. The maximum value of apparent nitrogen use efficiency was obtained with NPKSZn (54.06%) treatment which evidently suggested its resultness for the long term fertilized soil.