Nitrite-oxidizing Bacteria Research Articles

Influence of decreasing temperature on aerobic granular sludge - microbial community dynamics and treatment performance

Municipal wastewater in temperate climates is characterized by seasonal temperature changes. Temperature is a determining factor for biological processes, but the impact of gradually decreasing temperature on aerobic granular sludge (AGS) has been largely unexplored. In this study, the influence of decreasing temperature from 20 °C to 6 °C on AGS was investigated at rates of 0.5 and 1 °C per week. Temperature was a major driver for microbial community change, where the community response could be divided into three main subclusters. Strains within the guilds of ammonium- and nitrite-oxidising bacteria (AOB and NOB) as well as polyphosphate- and glycogen-accumulating organisms (PAOs and GAOs) grouped in different subclusters, indicating variable responses among and between the guilds. The phosphorous removal rate was sufficient for complete removal at all temperatures, presumably due to functional redundancy within the PAOs. The nitrification rate was, however, seriously impaired below 13 °C, despite diversity within AOB and NOB.

Towards low carbon demand and highly efficient nutrient removal: Establishing denitrifying phosphorus removal in anaerobic/anoxic/oxic + nitrification system

A two-sludge anaerobic/anoxic/oxic + nitrification system with simultaneous nitrogen and phosphorus removal was studied for enhanced low-strength wastewater treatment. After 158 days of operation, excellent NH4+-N, chemical oxygen demand (COD) and PO43−-P removal (99.0 %, 90.0 % and 92.0 %, respectively) were attained under a low carbon/nitrogen ratio of 5, resulting in effluent NH4+-N, COD and PO43−-P concentrations of 0.3, 30.0 and 0.5 mg/L, respectively. The results demonstrate that the anaerobic/anoxic/oxic sequencing batch reactor (A2-SBR) and nitrification sequencing batch reactor (N-SBR) had favorable denitrifying phosphorus removal and nitrification performance, respectively. High-throughput sequencing results indicate that the phosphate-accumulating organisms Dechloromonas (1.1 %) and Tetrasphaera (1.2 %) were enriched in the A2-SBR, while the ammonia-oxidizing bacteria Nitrosomonas (7.8 %) and the nitrite-oxidizing bacteria Nitrospira (18.1 %) showed excellent accumulation in the N-SBR. Further analysis via functional prediction revealed that denitrification is the primary pathway of nitrogen metabolism throughout the system. Overall, the system achieved low carbon and high efficiency nutrient removal.

High ammonia loading rate and biofilm reattachment initiated partial nitrification and anammox in a membrane aerated biofilm reactor

Inhibition of nitrite oxidizing bacteria (NOB) is important for efficient wastewater treatment via anammox. Here, a novel strategy was demonstrated, which based on the detachment and reattachment of the biofilm to establish partial nitrification and anammox in a membrane aerated biofilm reactor (MABR). The results showed that using free ammonia as the sole inhibitor to start up partial nitrification took more time, and NOB acclimated to the free ammonia inhibition. Detaching the biofilm made it possible for free ammonia to contact NOB in deeper zones of the biofilm while at the same time washing NOB out of the reactor. Nitrite accumulation efficiency (NAE) increased to 70 % in 50 days when both high ammonia loading and biofilm reattachment were applied. With a short hydraulic retention time and high concentrations of ammonia and nitrite, anammox activity appeared in the reactor and resulted in a nitrogen removal efficiency of 60 %. Microbial community analysis indicated that Nitrosomonas, Nitrospira, and Candidatus Brocadia were the main functional taxa. Anaerobic ammonia oxidizing bacteria (AnAOB) proliferated within the biofilm, inhibiting NOB growth by competing for nitrite. Endogenous denitrifying bacteria also grew, coexisting with AnAOB. Predicted abundance of genes related to fermentation correlated with those involved in denitrification, indicating the possibility of heterotrophic denitrification. These findings help further our understanding of biofilm control-based strategies for NOB management and have implications for establishing autotrophic nitrogen removal using MABRs.

Effect and potential mechanisms of sludge-derived chromium, nickel, and lead on soil nitrification: Implications for sustainable land utilization of digested sludge

Increasing occurrence of heavy metals (HMs) in sewage sludge threatens its widespread land utilization in China due to its potential impact on nutrient cycling in soil, requiring a better understanding of HM-induced impacts on nitrification. Herein, lab-scale experiments were conducted over 185-day, evaluating the effect of sludge-derived chromium (Cr3+), nickel (Ni2+), and lead (Pb2+) on soil nitrification at different concentrations. Quantitative polymerase chain reaction and linear regression results revealed an inhibitory sequence of gene abundance by HMs’ labile fraction: ammonia-oxidizing bacteria (AOB)-ammonia monooxygenase (amoA)> nitrite oxidoreductase subunit alpha (nxrA)> nitrite oxidoreductase subunit beta (nxrB). The toxicity of HMs’ incremental labile fraction decreased in the order of Ni2+>Cr3+>Pb2+, with respective threshold values of 5.01, 24.03 and 38.42 mg·kg−1. Furthermore, extending incubation time reduced HMs inhibition on ammonia oxidation, mainly related to their fraction bound to carbonate minerals. Random Forest analysis, variation partitioning analysis, and Mantel test indicated that soil physicochemical properties primarily affected nitrification genes, especially in the test of Cr3+ on AOB-amoA, nxrA, nxrB, Ni2+ for complete ammonia-oxidizing bacteria-amoA, and Pb2+ for nxrA and nxrB. These findings underline the importance of labile HMs fractions and soil physicochemical properties to nitrification, guiding the establishment of HM control standards for sludge utilization.

Dynamic pulse approach to enhancing mainstream Anammox process stability: Integrating sidestream support and tackling nitrite-oxidizing bacteria challenges

Nitrite-oxidizing bacteria (NOB) seriously threaten the partial nitritation and Anammox (PN/A) process, hindering its mainstream application. Herein, a one-stage PN/A reactor was continuously operated for 245 days under nitrogen loading rate lifted from 0.4 g N/L/d to 0.6 g N/L/d and 0.8 g N/L/d with the nitrogen removal efficiency of 71 %, 64 %, and 41 %, respectively. Furthermore, the NOB species over time was identified as Nitrospira_sp._OLB3, exhibiting an increase of the relative abundance from 0.9 % to 4.3 %. The hydroxyapatite (HAP) granules gradually lost their microbiological function of Anammox bacteria then aged, leading to NOB dominance. Therefore, one “pulse therapy” was introduced and combined with “continuous enhancement” of Anammox sludge supported by sidestream to competitively limit the NOB dynamics. The treatment's effect persisted for around two months. The strategy that returning at least 50 % of the impaired HAP granular sludge to the sidestream for recultivation could fulfill the bottlenecks of mainstream PN/A.

Partial nitrification response to dissolved oxygen variation and aerobic starvation: Kinetics and microbial community analyses

Partial nitrification (PN) has great significance in promoting both anaerobic ammonium oxidation (anammox) and advanced nitrogen removal. However, the kinetics of PN have not been sufficiently studied. Deterioration of PN often occurs due to nitrite-oxidizing bacteria (NOB) outgrowth, for which the dissolved oxygen (DO) is a critical factor. This study investigated the kinetic parameters of ammonia-oxidizing bacteria (AOB) and NOB by varying the DO concentration. The partial nitrification-anammox (PNA) process was operated under sequentially low (0.3–0.8 mg/L) and high (4.0–5.0 mg/L) DO. The results show that AOB could not adapt to low DO, resulting in a significant decrease in the maximum AOB activity (Vmax,AOB) and a slightly altered oxygen half-saturation constant of AOB (KO,AOB). Conversely, KO,NOB decreased and Vmax,NOB maintained, indicating that the main NOB genus, Nitrospira, was more adaptable to DO changes. Under high DO, AOB growth was weaker than NOB, leading to a low Vmax,AOB – Vmax,NOB value; KO,AOB increased and was larger than KO,NOB and PNA was eventually destroyed. The aerobic starvation was then adopted to restore PN. AOB recovered faster and dominated, with Vmax,AOB greatly exceeding Vmax,NOB. Finally, NOB were inhibited and PN was recovered. During this process, KO,NOB increased and was higher than KO,AOB. Changes in the kinetic parameters, especially the Vmax,AOB -Vmax,NOB value, have specific guiding significance for DO regulation during PNA. This work helps better understand the effect of DO variations on the nitrifying microbial community, which influences oxygen affinity and maximum activity.

Isolation of a Moderately Acidophilic Nitrobacter from a Nitrifying Community Supplied with Urea.

Nitrite-oxidizing bacteria (NOB), which perform the second step of aerobic nitrification, play an important role in soil. In the present study, we report a novel isolate from agricultural soil affiliated with the genus Nitrobacter and its physiological characteristics. We sampled the surface soil of a vegetable field and obtained mixed culture A31 using the most probable number (MPN) method with inorganic medium containing 0.75‍ ‍mM urea (pH 5.5). The dilution-extinction procedure on culture A31 led to the isolation of a strain that was designated as Nitrobacter sp. A67. The nxrB1 gene sequence of Nitrobacter sp. A67 (302 bp) was classified into Cluster 5, and the highest sequence identity was 96.10% with Nitrobacter sp. BS5/19. The NO2- oxidation activity of Nitrobacter sp. A67 was investigated at various pH. The optimum pH for NO2- oxidation was 5.8-6.4. This result indicates that Nitrobacter sp. A67 is a moderately acidophilic nitrite-oxidizing bacterium.

Effect of thermal shock and sustained heat treatment on mainstream partial nitrification and microbial community in sequencing batch reactors.

In order to develop a promising means of achieving mainstream short-cut nitrification, this study evaluated the effect of thermal shock on nitrite accumulation using intermittent offline and continuous inline heat treatment of biomass in sequencing batch reactors (SBRs). The SBRs fed with municipal wastewater were operated at a solid retention time of 7 days and nitrogen loading rate of 0.04 gN/L·d to 0.08 gN/L·d without the application of pre-treatment. Contrary to literature studies that showed suppression of nitrite-oxidizing bacteria at temperature 60 to 80 °C, nitrite accumulation was achieved temporarily when 20% of the biomass was heated for 2 h at 47 °C, as well as in continuously heated SBRs at 37 °C and 42 °C. The continuously heated reactors at 37 °C and 42 °C produced a maximum nitrite accumulation ratio (NAR) of 0.59 and 0.79, respectively, whereas the intermittent offline heating at 47 °C-2 h produced a NAR of 0.37. Although nitrite accumulation was stable only for 10-12 days in all heated reactors, this study demonstrates the achievement of mainstream partial nitrification (PN) at lower temperature (42 °C) than that reported in literature and also highlights the potential for achieving PN by implementing heat treatment of a portion of the return activated sludge (RAS) in biological nitrogen removal (BNR) systems. During the time when full nitrification was achieved, Nitrospira was more dominant than Nitrosomonas in all reactors at ratios of 1.4:1, 2.4:1, 2.4:1, and 3.7:1 for the control SBR (22 °C), 47 °C -2 h offline heating SBR, 37 °C SBR, and 42 °C SBR, respectively, suggesting that it may have played a role as a comammox bacteria capable of degrading ammonia to nitrates at elevated temperature.

Achieving partial nitrification in a continuous-flow bioreactor by free ammonia treatment combined with low dissolved oxygen

Partial nitrification (PN) is still difficult to achieve in continuous-flow bioreactors. In this study, free ammonia (FA) treatment combined with low dissolved oxygen (DO) were applied to achieve PN in an A/O bioreactor. Batch tests showed 200 mg/L FA with 12 h treatment time could effectively expand the activity difference between ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB), with an 24.86% falling of AOB activity and an 54.34% decrease of NOB activity. Furthermore, AOB activity can be recovered in a shorter time after once FA treatment than NOB. FA treatment combined with low DO could effectively establish AOB advantage and achieved PN with the nitrite accumulation ratio (NAR) with 86.95% and the ammonia removal efficiency (ARE) with 93.11% in our A/O reactor. FA treatment caused a decrease of extracellular polymeric substance (EPS) secretion, among which the protein reduction may be a key factor that prevented NOB activity recovered. Meanwhile, Nitrospira abundance with FA treatment once a day decreased by 79.1%, while Nitrosomonas abundance dropped by 16.4% only. Amo and hao abundance increased while nxr abundance reduced by 71.8%. The decreased abundance of Nitrospira and nxr was the key to achieve PN. This study demonstrated critical regulating factors for PN achievement in continuous-flow bioreactors using FA and DO, and may provide a new insight for PN in engineering applications.

Recurrent multi-stressor floc treatments with sulphide and free ammonia enabled mainstream partial nitritation/anammox

Selective suppression of nitrite-oxidising bacteria (NOB) over aerobic and anoxic ammonium-oxidising bacteria (AerAOB and AnAOB) remains a major challenge for mainstream partial nitritation/anammox implementation, a resource-efficient nitrogen removal pathway. A unique multi-stressor floc treatment was therefore designed and validated for the first time under lab-scale conditions while staying true to full-scale design principles. Two hybrid (suspended + biofilm growth) reactors were operated continuously at 20.2 ± 0.6 °C. Recurrent multi-stressor floc treatments were applied, consisting of a sulphide-spiked deoxygenated starvation followed by a free ammonia shock. A good microbial activity balance with high AnAOB (71 ± 21 mg N L−1 d−1) and low NOB (4 ± 17 % of AerAOB) activity was achieved by combining multiple operational strategies: recurrent multi-stressor floc treatments, hybrid sludge (flocs & biofilm), short floc age control, intermittent aeration, and residual ammonium control. The multi-stressor treatment was shown to be the most important control tool and should be continuously applied to maintain this balance. Excessive NOB growth on the biofilm was avoided despite only treating the flocs to safeguard the AnAOB activity on the biofilm. Additionally, no signs of NOB adaptation were observed over 142 days. Elevated effluent ammonium concentrations (25 ± 6 mg N L−1) limited the TN removal efficiency to 39 ± 9 %, complicating a future full-scale implementation. Operating at higher sludge concentrations or reducing the volumetric loading rate could overcome this issue. The obtained results ease the implementation of mainstream PN/A by providing and additional control tool to steer the microbial activity with the multi-stressor treatment, thus advancing the concept of energy neutrality in sewage treatment plants.

Influence of Light Irradiation on Nitrification in Microalgal–Bacterial Systems for Treating Wastewater

The use of bacterial and microalgal consortia to remove nitrogen from wastewater has garnered attention as a potential alternative to conventional systems. This approach not only reduces energy consumption but also aids in nutrient recovery. Light is essential for algae photosynthesis; however, nitrifying bacteria are also influenced by light radiation. This mini-review summarizes the current knowledge concerning photoinhibition, the light stimulation of ammonia-oxidizing bacteria (AOB), resistance to light radiation, the implementation of microalgal–bacterial systems, and the possible mechanisms involved. Nitrosomonadaceae AOB and Nitrospiraceae nitrite-oxidizing bacteria (NOB) often coexist in a microalgal–bacterial system. Studies have suggested that AOB can tolerate light radiation at 200 μmol m−2·s−1 in microalgal–bacterial systems, whereas NOB are almost completely suppressed, which can result in partial nitrification in the bioreactor. An appropriate light level can stimulate AOB growth in microalgal–bacterial granular reactors and may improve algae metabolic activity. Granular sludges or artificial “light-shielding hydrogel” could effectively protect nitrifying bacteria from light intensities up to 1600 μmol m−2·s−1 in wastewater treatment reactors. Microalgal–bacterial systems along with the associated “algal shading effect” have been widely used in pond aquaculture. This approach minimizes the need for costly mechanical aeration through photo-oxygenation and facilitates nutrient recovery by filter-feeding fish.

How to Provide Nitrite Robustly for Anaerobic Ammonium Oxidation in Mainstream Nitrogen Removal.

Innovation in decarbonizing wastewater treatment is urgent in response to global climate change. The practical implementation of anaerobic ammonium oxidation (anammox) treating domestic wastewater is the key to reconciling carbon-neutral management of wastewater treatment with sustainable development. Nitrite availability is the prerequisite of the anammox reaction, but how to achieve robust nitrite supply and accumulation for mainstream systems remains elusive. This work presents a state-of-the-art review on the recent advances in nitrite supply for mainstream anammox, paying special attention to available pathways (forward-going (from ammonium to nitrite) and backward-going (from nitrate to nitrite)), key controlling strategies, and physiological and ecological characteristics of functional microorganisms involved in nitrite supply. First, we comprehensively assessed the mainstream nitrite-oxidizing bacteria control methods, outlining that these technologies are transitioning to technologies possessing multiple selective pressures (such as intermittent aeration and membrane-aerated biological reactor), integrating side stream treatment (such as free ammonia/free nitrous acid suppression in recirculated sludge treatment), and maintaining high activity of ammonia-oxidizing bacteria and anammox bacteria for competing oxygen and nitrite with nitrite-oxidizing bacteria. We then highlight emerging strategies of nitrite supply, including the nitrite production driven by novel ammonia-oxidizing microbes (ammonia-oxidizing archaea and complete ammonia oxidation bacteria) and nitrate reduction pathways (partial denitrification and nitrate-dependent anaerobic methane oxidation). The resources requirement of different mainstream nitrite supply pathways is analyzed, and a hybrid nitrite supply pathway by combining partial nitrification and nitrate reduction is encouraged. Moreover, data-driven modeling of a mainstream nitrite supply process as well as proactive microbiome management is proposed in the hope of achieving mainstream nitrite supply in practical application. Finally, the existing challenges and further perspectives are highlighted, i.e., investigation of nitrite-supplying bacteria, the scaling-up of hybrid nitrite supply technologies from laboratory to practical implementation under real conditions, and the data-driven management for the stable performance of mainstream nitrite supply. The fundamental insights in this review aim to inspire and advance our understanding about how to provide nitrite robustly for mainstream anammox and shed light on important obstacles warranting further settlement.

Genomic insight into strategy, interaction and evolution of nitrifiers in metabolizing key labile-dissolved organic nitrogen in different environmental niches.

Ammonia-oxidizing archaea (AOA) and bacteria (AOB), nitrite-oxidizing bacteria (NOB), and complete ammonia oxidizers (comammox) are responsible for nitrification in nature; however, some groups have been reported to utilize labile-dissolved organic nitrogen (LDON) for satisfying nitrogen demands. To understand the universality of their capacity of LDON metabolism, we collected 70 complete genomes of AOA, AOB, NOB, and comammox from typical environments for exploring their potentials in the metabolism of representative LDON (urea, polyamines, cyanate, taurine, glycine betaine, and methylamine). Genomic analyses showed that urea was the most popular LDON used by nitrifiers. Each group harbored unique urea transporter genes (AOA: dur3 and utp, AOB: utp, and NOB and comammox: urtABCDE and utp) accompanied by urease genes ureABC. The differentiation in the substrate affinity of these transporters implied the divergence of urea utilization efficiency in nitrifiers, potentially driving them into different niches. The cyanate transporter (cynABD and focA/nirC) and degradation (cynS) genes were detected mostly in NOB, indicating their preference for a wide range of nitrogen substrates to satisfy high nitrogen demands. The lack of genes involved in the metabolism of polyamines, taurine, glycine betaine, and methylamines in most of nitrifiers suggested that they were not able to serve as a source of ammonium, only if they were degraded or oxidized extracellularly as previously reported. The phylogenetic analyses assisted with comparisons of GC% and the Codon Adaptation Index between target genes and whole genomes of nitrifiers implied that urea metabolic genes dur3 and ureC in AOA evolved independently from bacteria during the transition from Thaumarchaeota to AOA, while utp in terrestrial AOA was acquired from bacteria via lateral gene transfer (LGT). Cyanate transporter genes cynS and focA/nirC detected only in a terrestrial AOA Candidadus Nitrsosphaera gargensis Ga9.2 could be gained synchronously with Nitrospira of NOB by an ancient LGT. Our results indicated that LDON utilization was a common feature in nitrifiers, but metabolic potentials were different among nitrifiers, possibly being intensely interacted with their niches, survival strategies, and evolutions.

Rapid start-up of an innovative pilot-scale staged PN/A continuous process for enhanced nitrogen removal from mature landfill leachate via robust NOB elimination and efficient biomass retention

The start-up and stable operation of partial nitritation-anammox (PN/A) treatment of mature landfill leachate (MLL) still face challenges. This study developed an innovative staged pilot-scale PN/A system to enhance nitrogen removal from MLL. The staged process included a PN unit, an anammox upflow enhanced internal circulation biofilm (UEICB) reactor, and a post-biofilm unit. Rapid start-up of the continuous flow PN process (full-concentration MLL) was achieved within 35 days by controlling dissolved oxygen and leveraging free ammonia and free nitrous acid to selectively suppress nitrite-oxidizing bacteria (NOB). The UEICB was equipped with an annular flow agitator combined with the enhanced internal circulation device of the guide tube, which achieved an efficient enrichment of Candidatus Kuenenia in the biofilm (relative abundance of 33.4 %). The nitrogen removal alliance formed by the salt-tolerant anammox bacterium (Candidatus Kuenenia) and denitrifying bacteria (unclassified SBR1031 and Denitratisoma) achieved efficient nitrogen removal of UEICB (total nitrogen removal percentage: 90.8 %) and at the same time effective treatment of the refractory organic matter (ROM). The dual membrane process of UEICB fixed biofilm combined with post-biofilm is effective in sludge retention, and can stably control the effluent suspended solids (SS) at a level of less than 5 mg/L. The post-biofilm unit ensured that effluent total nitrogen (TN) remained below the 40 mg/L discharge standard (98.5 % removal efficiency). Compared with conventional nitrification-denitrification systems, the staged PN/A process substantially reduced oxygen consumption, sludge production, CO2 emissions and carbon consumption by 22.8 %, 67.1 %, 87.1 % and 87.1 %, respectively. The 195-day stable operation marks the effective implementation of the innovative pilot-scale PN/A process in treating actual MLL. This study provides insights into strategies for rapid start-up, robust NOB suppression, and anammox biomass retention to advance the application of PN/A in high-ammonia low-carbon wastewater.

Oxygen affinity and light intensity induced robust phosphorus removal and fragile ammonia removal in a non-aerated bacteria-algae system

Non-aerated bacteria-algae system gaining O2 through photosynthesis presents an alternative for costly mechanical aeration. This study investigated oxygen supply and performance of nutrients removal at low and high light intensity (LL and HL). The results showed that P removal was high and robust (LL 97 ± 1.8 %, HL 95 % ± 2.9 %), while NH4+-N removal fluctuated dramatically (LL 66 ± 14.7 %, HL 84 ± 8.6 %). Oxygen generated at illumination of 200 μmol m−2 s−1, 6 h was sufficient to sustain aerobic phase for 2.25 g/L MLSS. However, O2 produced by algae was preferentially captured in the order of heterotrophic bacteria (HB), ammonia oxidizing bacteria (AOB), nitrite oxidizing bacteria (NOB). Oxygen affinity coupled with light intensity led to NOB suppression with stable nitrite accumulation ratio of 57 %. Free nitrous acid (FNA) and light stimulated the abundance of denitrifying polyphosphate accumulating organism (DPAO) of Flavobacterium, but with declined P-accumulating metabolism (PAM) of P release, P/C, K/P and Mg/P ratios. Flavobacterium and cyanobacteria Leptolyngbya, along with biologically induced CaP in extracellular polymeric substances was the key to robust P removal. AOB of Ellin6067 and DPAO of Flavobacteria offer a promising scenario for partial nitrification-denitrifying phosphorus removal.

Single-stage partial nitrification/anammox process performance restoration by bio-augmenting nitrifiers with or without nitrite oxidizing bacteria suppression

The use of low dissolved oxygen (DO) is common to suppress the growth of nitrite oxidizing bacteria (NOB) in the partial nitrification/anammox (PN/A) process. However, this approach can negatively impact the activity of ammonium oxidizing bacteria (AOB) and reduce the oxygen consumption rate, disrupting the essential low DO environment required for anammox bacteria (AMX) and leading to a decline in PN/A performance. We innovatively harnessed the strategy of common nitrifiers (AOB + NOB) bioaugmentation in an impaired single-stage PN/A reactor after operation for a period of time. The results demonstrated an immediate improvement, with the total nitrogen removal efficiency increasing from approximately 60 % to 80 %. This enhancement was attributed to decreased NOB activity and increased AMX activity, which occurred at reduced DO level (0.24 to 0.12 mg O2/L) following nitrifiers bioaugmentation. Partial nitrification (PN) and PN/A batch experiments with nitrifiers bioaugmentation further supported these findings. This strategy pioneered a fresh perspective that the diversion of nitrite from NOB to AMX was the pivotal underlying mechanism. Mathematical simulations complemented these results by demonstrating that nitrifiers bioaugmentation broadened the optimal oxygen supply window.

Newly Discovered Bacteria Help Keep Ecosystems in Balance

Nitrogen is essential to all life on Earth. Tiny organisms, called microbes, help to make nitrogen available to all other living things. One group of microbes, called nitrite-oxidizing bacteria (NOB), help to remove excess nitrogen from the environment that can be dangerous to plants and animals. However, there is still a lot to learn about the distribution and function of NOB in nature. In the lab, we grew new types of NOB from rivers. By studying their DNA, we found that these NOB can gain energy in many ways and survive the stress of pollution. Similar NOB seem to be present all over Earth. Studying NOB can help us to understand how changes to the environment might affect microbes and their habitats.

Temperate grassland soil nitrifiers are more sensitive to nitrogen addition than simulated warming

Soil nitrogen transformations are influenced by both global climate change and human activities. Nitrification, affected by ammonia-oxidizing and nitrite-oxidizing microorganisms, is one of the crucial N transformation processes. However, the impact of environmental change on the diversity and community structure of these microorganisms in grassland ecosystems remains poorly understood. This study explored the alpha diversity and community structures of N-cycling microorganisms in response to simulated warming and N addition in a temperate steppe. The results showed that N addition significantly increased the taxon richness of ammonia-oxidizing bacteria and nitrite-oxidizing bacteria but significantly decreased the diversity of total archaea. Also, N addition significantly changed the community structures of ammonia-oxidizing bacteria, nitrite-oxidizing bacteria, and total archaea and bacteria. However, warming had no effect on the whole microbiome alpha and beta diversity. The alternations of microbial communities were mainly induced by changes in soil pH and inorganic N concentrations. This study illustrates that the temperate grassland microbial communities are more sensitive to N addition than simulated warming under future climate change scenes.

Effects of Nitrogen Fertilizer on Nitrospira- and Nitrobacter-like Nitrite-Oxidizing Bacterial Microbial Communities under Mulched Fertigation System in Semi-Arid Area of Northeast China

The accumulation of nitrite is frequently overlooked, despite the fact that nitrification is the most essential phase of the entire nitrogen (N) cycle and that nitrifying bacteria play a significant role in nitrification. At present, the effects of different N application rates on soil nitrite-oxidizing bacteria (NOB) abundance, community composition, diversity, and its main influencing factors are still unclear. In this study, five N fertilizer application rates under film mulching and a drip irrigation system were studied in the semi-arid area of Northeast China. The treatments were 0 kg ha−1 (N0), 90 kg ha−1 (N1), 150 kg ha−1 (N2), 210 kg ha−1 (N3), and 270 kg ha−1 (N4). Fluorescent quantitative PCR and Illumina Miseq sequencing were used to analyze the abundance and community structure of NOB under different amounts of N application. The results showed that the increase in amounts of N application was strongly accompanied by an increase in the content of soil organic matter (SOM), total nitrogen (TN), nitrate nitrogen (NO3−-N), and ammonium nitrogen (NH4+-N), while the pH significantly reduced with an increase in N fertilization. N fertilization significantly increased soil nitrite oxidoreductase (NXR) activity, soil nitrification potential (PNR), and soil nitrite oxidation potential (PNO). A high N application rate significantly heightened the abundance of Nitrospira- and Nitrobacter-like NOB. N fertilizer considerably raised the Shannon index of Nitrospira-like NOB. The N application amount was the key factor affecting the community structure of Nitrospira-like NOB, and available nitrogen (AN) had the dominant influence on the community structure of Nitrospira-like NOB. N fertilizer can cause soil acidification, which affects NOB abundance and diversity. Nitrospira-like NOB may promote nitrite oxidation in different N application rates under a mulched fertigation system. The findings offered a crucial scientific foundation for further investigation into how nitrite-oxidizing bacteria respond to N fertilizer management strategies in farmland soil under film mulching drip irrigation in Northeast China.

Effect of antibiotics addition on nutrient removal stability and microbial community change of the solar-powered oxidation ditch-membrane bioreactor in treating building wastewater

The solar-powered oxidation ditch-membrane bioreactors (SOD-MBR) system was developed and operated with long solid retention times (SRTs) of 80 and 160 days. The aim was to investigate the effects of using a long SRT and antibiotics in building wastewater on the stability of nutrient removal, as well as membrane fouling. An increase in the SRT from 80 days to 160 days did not significantly affect the performance of the SOD-MBR system. Ciprofloxacin and Sulfamethoxazole removal efficiencies were 94.47 ± 1.54% and 87.54 ± 24.7%. However, the presence of antibiotics resulted in lower removal efficiencies for NH4+-nitrogen and phosphorus and stimulated the production of extracellular polymeric substances (EPS), particularly proteins in L-EPS and T-EPS of the foulant. FTIR and FEEM analysis revealed that the microbial sludge primarily consisted of proteins, carbohydrates, and lipids. Furthermore, the relative abundance analysis of microbial communities identified bacteria associated with nitrogen removal in the SOD-MBR system, including Anammox, AOB (ammonia oxidizing bacteria), DNB (denitrifying bacteria), and NOB (nitrite oxidizing bacteria), with a total of 25 genera. The majority of these bacteria were stimulated by the presence of antibiotics, resulting in higher relative abundance. Finally, the SOD-MBR system achieved energy savings of 97.38% by utilizing photovoltaic (PV) technology.