We determined the different parameters of antioxidant activity with different solvents of polarities in sequence of raising polarity i.e., pet-ether, chloroform, ethyl acetate, methanolic and aqueous extracts of Safoof-e-Tabkheer. Potential antioxidant was detected through DPPH, nitric oxide and metal chelating test. MeSt and AqSt extract had important antioxidant activity in compared to all extracts, by values of IC50=82.31±2.16μg/mL, IC50=98.65±1.47μg/mLin the DPPH and IC50=84.28±1.54μg/mL, IC50=72.14±1.04μg/mL in nitric oxide assays, and IC50=233.38±2.39μg/mL, IC50=98.65±1.47μg/mLmetal chelating test. The total amount of phenol and flavonoid quantified wereMeSt194.7±1.15mg/g and AqSt 149.4±1.64mg/g of gallic acid equivalent per gram respectively and MeSt152.4±1.12 mg/g and AqSt 105.01±1.13mg/gof rutin equivalent respectively. Antibacterial, antifungal and MIC were calculated. MeSt and AqSt prohibited the growth of Gram-negative and Gram-positive bacteria, at MIC of 8-64 µg/ml. Antifungal activity at MIC was 16-128 µg/ml. MeSt and AqSt exhibited cytotoxicity against MTT assay. These results suggest that MeSt and AqSt extract has potential cytotoxic, antimicrobial and antioxidant potential that support the ethnopharmacological uses of Safoof-e-Tabkheer.