The effects of aqueous pod extract of Acacia nilotica was investigated in rats. The pod extract of A. nilotica was obtained by Soxhlet extraction using distilled water as a solvent. The aqueous extract was administered for a period of 21 days at 50, 100 and 200 and 400 mg/kg body weight, respectively. The liver enzymes and some biochemical parameters were evaluated. The tissue samples were also taken for histopathological preparations. The effect of the extract significantly (P 0.05) decreased values of serum albumin, total protein, glucose and triglyceride at the end of the 21 days. While alkaline phosphatase (ALP) and Urea values were not affected. The histopathological study revealed marked haemorrhages of lung, heart and kidney with renal tubular degeneration. Liver revealed hepatocellular necrosis and mononuclear cell infiltrations, mild congestion of the stomach and mild sub mucosal mononuclear cell infiltrations of the small intestine, respectively. It was therefore concluded that A. nilotica extract has the potential to produce some level of toxicities and it consumption for medicinal purposes should be discourage. Key words: Histopathological features, A. nilotica aqueous pod extract, biochemical parameters.