Nigrum Extract Research Articles

Black pepper extract reduces tumour incidence via suppressing tumour-promoted cytokines/chemokines and modulating T cell subtype

ABSTRACT Our study aimed to evaluate tumour retardation effects of low-piperine fractional Piper nigrum extract (PFPE or LP-PE) related to enhancement of immune response. Results showed that PFPE at doses of 50 (low), 100 (intermediated) and 150 (high) mg/kg BW had a tumour incidence of 20, 20 and 0%, respectively. PFPE at low dose inhibited cytokines/chemokines in the early stages of cancer however it could not suppress longer than 3 months. Intermediated dose of PFPE inhibited cancer cytokines/chemokines including sICAM-1, CX3CL1, CXCL7, VEGF, TIMP-1, CCL5, CCL20, CXCL1, CXCL5, CXCL9 and IL-1β. Interestingly, PFPE stimulated IFN- γ at 3 months after treatment in all doses. Intermediated and high doses of PFPE increased Th1 and reduced Th2. Moreover, only a high dose of PFPE reduced Treg. Additionally, PFPE reduced ER-α and NF-κB and increased IL-6R and STAT3 proteins in tumour tissues. Our findings indicate that PFPE suppressed breast cancer via immune modulation.

Hybrid Albumin-Decorated Lipid-Nanocarrier-Mediated Delivery of Polyphenol-Rich Sambucus nigra L. in a Potential Multiple Antitumoural Therapy.

The current research attempted to address the suitability of bioactive Sambucus nigra extract entrapped in albumin-decorated nanostructured lipid carriers (NLCs) as a promising "adjuvant" in improving tumour penetration for multiple antitumour therapy. The new hybrid albumin-decorated NLCs were characterised based on, e.g., the particle size, zeta electrokinetic potential, SambucusN entrapment efficiency, and fluorescence spectroscopy and tested for different formulation parameters. The antioxidant activity of NLC-SambucusN was significantly enhanced by a bovine serum albumin (BSA) polymer coating. According to the real-time cell analysis (RTCA) results, NLC-I-SambucusN-BSA behaved similarly to the chemotherapeutic drug, cisplatin, with cell viability for LoVo tumour cells of 21.81 ± 1.18%. The new albumin-NLC-SambucusN arrested cancer cells in G1 and G2 cycles and intensified the apoptosis process in both early and late phases. An advanced induction, over 50% apoptosis in LoVo colon cells, was registered for 50 μg/mL of NLC-II-SambucusN-BSA, a fourfold increase compared to that of untreated cells. RTCA and flow cytometry results showed that concentrations of the hybrid NLC-SambucusN up to 50 μg/mL do not affect the proliferation of normal HUVEC cells. This approach provides insightful information regarding the involvement of phytochemicals in future therapeutic strategies. Albumin-decorated NLCs can be considered a noteworthy strategy to be connected to antitumour therapeutic protocols.

Separation of major bioactive components by GC-MS and antioxidant studies on Solanum nigrum extract

Separation of major bioactive components by GC-MS and antioxidant studies on Solanum nigrum extract

DIETARY EFFECTS OF TULSI LEAVES AND BLACK PEPPER EXTRACT ON BROILER GROWTH PERFORMANCE AND CARCASS TRAITS

This study was conducted to determine the efficacy of the dietary effect of different levels of Tulsi leaves (Ocimum sanctum) and Black Pepper (Piper nigrum) Extract (TBE) on growth performance and carcass traits of commercial broiler. The study was conducted at the Poultry farm of Hajee Mohammad Danesh Science and Technology University, Dinajpur during the period from 11 April to 08 May, 2019. A total of 96 day old broiler chicks (Lohman Meat) were randomly and equally distributed to 4 groups having 3 replicates of 8 birds each. The birds were grouped as T0, T1, T2 and T3 group with normal water (control group), 1% TBE 1ml /L of drinking water, 2% TBE 2 ml/L of drinking water and 3% TBE 3ml /L of drinking water, respectively. Body weight gains, Feed Conversion Ratio (FCR), mortality (%) and meat yield traits were recorded. The collected data were analyzed by using ANOVA with SPSS version 22. Feed intake (g/bird) was almost similar (P>0.05) among the dietary treatment groups. Body weight gain (g) and FCR were significantly (P<0.05) differed among the dietary treatment groups. The highest body weight gain (g) was significantly (P<0.05) higher in T2 (1659.35g±2.19), followed by T1 (1631.05g±2.52), T3 (1590.67g±2.73) and T0 (1472.01g±4.58), respectively. The lowest FCR was found in T2 (1.41±0.00) and the highest FCR in T0 (1.52±0.01), the intermediate in T1 (1.45±0.01) and T3 (1.46±0.01), respectively. It was found that there was significant (P<0.05) difference among the dietary treatment groups in case of live weight (g), carcass weight (g),dressing percentage (%), breast weight (g), liver weight(g), but there was almost similar (P>0.05) among the dietary treatment groups in case of heart weight (g), spleen weight (g), gizzard weight (g), thigh weight (%), head weight (g), intestine weight (g) and shank weight (g). The carcass meat weight, Carcass yield (%), breast weight and breast meat weight (%) was significantly (P<0.05) higher in treatment T2 group compared to control group T0. Liver weight was significantly (P<0.05) higher in control group T0 compared to treatment group T2. No mortality was found among the dietary treatment groups during experimental period. Based on the result it could be concluded that 2% Tulsi leaves and Black Pepper Extract (TBE) 2 ml/L of drinking water may be used in broiler diet as growth promoter and it may also serve as an antioxidant.

Chemical Profile and Potential Application of Agri-food Waste Products for Counteracting Diabetes Induced Neuropathy in Rats.

This study aimed to prepare defatted ethanol extract of Abelmoschus esculentus leaves, Morus nigra leaves and Punica granatum peel, to identify the chemical composition of these extracts and to explore their efficacy in counteracting diabetic neuropathy. LC-ESI-MS spectrometry was the hyphenated tool for component identification of these extracts. Behavioral, biochemical, and histopathological investigations were carried out after treatments of diabetic rats. The phenolic contents in the extracts are 16.38, 34.75 and 40.57 mg GAE/g extract regarding A. esculentus leaves, M. nigra leaves and P. granatum peel respectively. Chemodiversity of the phenolic contents was observed from the LC/Mass, where A. esculentus extract contained isoflavonoids and flavanones, M. nigra extract consisted of benzofurans, prenylated flavonoids, stilbenes, and xanthones, and P. granatum extract was rich in ellagitanins, condensed tannins, and anthocyanins. The extracts normalize of blood glucose levels, enhance the explorative behavior of the rats and their response time to thermal pain, restore the oxidant/antioxidant balance, attenuate inflammation, augment brain monoamines levels and modulate MAO-A and Ache enzyme activity. Furthermore, they recovered brain histopathological alterations. Conclusively, this study offers experimental evidence for the neuroprotective impact of studied defatted ethanol extracts against diabetic neuropathy via their hypoglycemic effect, antioxidant activity, and anti-inflammatory potential.

Antidepressant-like and antioxidant effects of Morus nigra L. in rats subjected to the unpredictable chronic mild stress model

This study aimed to evaluate the antidepressant-like and antioxidant potential of the aqueous leaf extract of Morus nigra L. in rats subjected to unpredictable chronic mild stress (UCMS). Male Wistar rats were divided into four groups, control and stressed, and treated with saline or aqueous extract of M. nigra. The dose administered was 10 mg/kg daily by gavage for 5 weeks concurrently with UCMS. M. nigra extract which showed syringic acid, quercetin, and rutin, as major phenolic compounds decreased the immobility time without changes in locomotor activity of stressed rats, in the forced swimming and open field tests, respectively. In addition, the extract diminished carbonylated proteins, lipid peroxidation, and nitrite levels in the brains of rats subjected to UCMS. These data reinforced antidepressant-like properties with the involvement of oxidative stress modulation by M. nigra extract pointing it as a possible new adjuvant treatment for depression.

Green Synthesis of Copper Oxide Nanoparticles from Waste Solar Panels Using Piper nigrum Fruit Extract and Their Antibacterial Activity

To promote environmental development and sustain resource circularity, recycling metals from electronic waste is essential. Electronic waste is a significant secondary source of metals, with its production increasing rapidly and most remaining unrecycled. In solar panels, copper is the second-most-valuable metal after silver. We propose an innovative method to recycle copper from waste solar panels and convert it into copper oxide nanoparticles (CuONPs) using a green synthesis method. Synthesizing CuONPs is advantageous due to their large surface area compared to bulk material. Nitric acid, a strong oxidizing agent, was used to leach copper from solid copper wires in waste solar panels. A green synthesis method, following a bottom-up approach, was employed using Piper nigrum fruit extract to synthesize CuONPs. The synthesized nanoparticles were characterized using various qualitative and quantitative techniques. Spectroscopic analysis confirmed the formation of CuONPs, and transmission electron microscopy revealed that the nanoparticles were spherical with sizes up to 60 nm. Biomolecules from the Piper nigrum extract were detected on the surface of the crystalline CuONPs. These nanoparticles exhibited antibacterial activity against Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus using a well-diffusion method, showing a larger zone of inhibition for E. coli compared to S. aureus. This research demonstrates the complete recovery of copper from waste solar panels and its conversion into useful CuONPs, which have potential medicinal applications.

GC-MS and RP-HPLC Analysis Reveals Phytochemical Compositions and Antioxidant Potential in Solanum Schimperianum, Solanum Cordatum, and Solanum Nigrum Extracts from Saudi Arabia

GC-MS and RP-HPLC Analysis Reveals Phytochemical Compositions and Antioxidant Potential in Solanum Schimperianum, Solanum Cordatum, and Solanum Nigrum Extracts from Saudi Arabia

Solanum nigrum Toxicity and Its Neuroprotective Effect Against Retinal Ganglion Cell Death Through Modulation of Extracellular Matrix in a Glaucoma Rat Model.

Purpose: Glaucoma is a complex degenerative optic neuropathy characterized by loss of retinal ganglion cells (RGCs) leading to irreversible vision loss and blindness. Solanum nigrum has been used for decades in traditional medicine system. However, no extensive studies were reported on its antiglaucoma properties. Therefore, this study was designed to investigate the neuroprotective effects of S. nigrum extract on RGC against glaucoma rat model. Methods: High performance liquid chromatography and liquid chromatography tandem mass spectrometry was used to analyze the phytochemical profile of aqueous extract of S. nigrum (AESN). In vitro, {3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide} (MTT) and H2DCFDA assays were used to determine cell viability and reactive oxygen species (ROS) production in Statens Seruminstitut Rabbit Cornea cells. In vivo, AESN was orally administered to carbomer-induced rats for 4 weeks. Intraocular pressure, antioxidant levels, and electrolytes were determined. Histopathological and immunohistochemical analysis was carried out to evaluate the neurodegeneration of RGC. Results: MTT assay showed AESN exhibited greater cell viability and minimal ROS production at 10 μg/mL. Slit lamp and funduscopy confirmed glaucomatous changes in carbomer-induced rats. Administration of AESN showed minimal peripheral corneal vascularization and restored histopathological alterations such as minimal loss of corneal epithelium and moderate narrowing of the iridocorneal angle. Immunohistochemistry analysis showed increased expression of positive BRN3A cells and decreased matrix metalloproteinase (MMP)-9 activation in retina and cornea, whereas western blot analysis revealed downregulation of extracellular matrix proteins (COL-1 and MMP-9) in AESN-treated rats compared with the diseased group rats. Conclusions: AESN protects RGC loss through remodeling of MMPs and, therefore, can be used for the development of novel neurotherapeutics for the treatment of glaucoma.

Potential molluscicidal activity of the aqueous extracts of some plants and their powders against terrestrial snail Monacha obstructa (L. Pfeiffer, 1842) under laboratory and field conditions

As it is known that the excessive use of pesticides causes many environmental problems, the effects of four aqueous plant extracts and their powders [Solanum nigrum L., Withania somnifera (L.) Dunal, Salix mucronata Thunb. and Lawsonia inermis L.] were evaluated as natural molluscicides on the land snail Monacha obstructa (Family: Hygromiidae) under laboratory conditions. Three different bioassay methods were used: contact, leaf-dipping and bait techniques. The results indicated that, using all methods, S. Nigrum extract was the most toxic extract for the terrestrial snail M. obstructa. The contact technique of the tested plant extracts was the most effective method of application compared to other methods. Moreover, using plant extracts was better than using powders of these plants for controlling the terrestrial snail M. obstructa. In addition, the results indicated that the aqueous extract of S. nigrum gave the highest percentage of reduction in the snail population when assessed under field conditions. The obtained data showed that plant extracts were significantly effective against the terrestrial snail and could be used as alternatives to pesticides in integrated pest management.

Green synthesis of silver nanoparticles using mature-pseudostem extracts of Alpinia nigra and their bioactivities

Abstract Green synthesis of silver nanoparticles (AgNPs) employing agricultural wastes as plant extracts to improve environmental benignity and also economic value added is the highlight of this research. The mature pseudostem of Alpinia nigra is an unbeneficial raw material discarded from several food ingredients and medicinal formulas. Therefore, this research focused on condition optimization for AgNP synthesis with ecofriendly techniques using A. nigra mature-pseudostem extracts and evaluation of their antioxidant, antibacterial activities, and toxicity with brine shrimp lethality assay (BSLA). The optimal reaction conditions were achieved by using 5 mM silver nitrate (AgNO3) solution with a volume ratio of 2:8 for the extract to AgNO3 at pH 12 under room temperature. The morphology and crystalline phase of the generated AgNPs were characterized using UV–visible spectrophotometry, field emission-scanning electron microscope (FE-SEM), energy dispersive X-ray, X-ray diffraction, and Fourier transform-infrared (FTIR) techniques. The FE-SEM analysis exposed spherical shapes with an average diameter of approximately 49 nm. The XRD analysis indicated their face center cubic structure, and the FTIR spectra confirmed that phytochemicals from A. nigra extract promoted the synthesis of AgNPs. In particular, the biosynthesized AgNPs presented potential antibacterial activity against both Staphylococcus aureus and Escherichia coli and effective antioxidant capacity using the DPPH radical scavenging assay. Additionally, non-toxic desired AgNPs were confirmed with BSLA.

Total Phenolic content and Potential of Black Betel Leaf (Piper betle L.var Nigra) Extract as an Antioxidant and Anti-Gout

Research to find antioxidants and anti-gout has been done using black betel leaf. The sample was extracted at room temperature for 30 minutes using the Ultrasound-Assisted Extraction (UAE) method, and a yield of 8.47% was obtained. Phytochemical screening was carried out on the ethanol extract of black betel leaves and showed the presence of secondary metabolite compounds consisting of alkaloids, phenolics, saponins, tannins, and steroid glycosides, with a total phenolic content of 758,534±0.003mg GAE per gram of sample. Antioxidant activity analysis was carried out using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP) methods, which showed IC50 and EC50 values of 115.562±0.14mg/L and 119.273±0.385 mg/L, respectively. The antioxidant activity of black betel leaf extract is in the medium category (100–150 mg/L). The anti-gout activity test was carried out using allopurinol as a control, which showed the ability of black betel leaf extract to reduce uric acid up to 14.48±0.01%. The ability of black betel leaf extract as an anti-uric acid is five times better than allopurinol, which is commonly used as a gout drug. The research results obtained opened up the opportunity to find a natural medicine for gout.

Investigation of inhibition kinetics of various plant extracts on polyphenol oxidase enzyme from Paulownia Tomentosa and binding mechanism by molecular docking

Polyphenol oxidase/tyrosinase enzyme, which contains binuclear copper clusters play an important role in melanogenesis and enzymatic browning. Therefore, its inhibitors can be attractive in cosmetics and medicinal industries as depigmentation agents and also in food and agriculture industries as antibrowning compounds. Our aim in this study is to find natural plant extracts that will inhibit the polyphenol oxidase enzyme. The inhibitory effects of Verbascum thapsus, Tanacetum vulgare, Solanum nigrum and Datura stramonium herbal plant extracts obtained from Kosovo on the polyphenol oxidase enzyme activity were investigated. Polyphenol oxidase from the Poulownia tomentosa plant was purified using the three-phase purification method. It is determined that T. vulgare extract showed the most effective inhibitory effect with 0.43 mg/mL IC50 value. In order to explain and support the binding mechanism of the best inhibitor extract, the phenolic content of the T. vulgare extract was determined by LC-MS/MS and the mechanism of the inhibitory effect was explained by performing a molecular docking study for the phenolic compounds it contained at the highest rate.

THE ENDOPHYTIC FUNGUS EPICOCCUM NIGRUM: ISOLATION, MOLECULAR IDENTIFICATION AND STUDY ITS ANTIFUNGAL ACTIVITY AGAINST PHYTOPATHOGENIC FUNGUS FUSARIUM SOLANI

Fusarium solani is a soil-borne fungus that causes fusarium dry rot disease in Upper Egypt. In this study, endophytic fungi from the Helwan University campus were collected, separated, and classified according to their morphological traits. Based on morphological and molecular characteristics, Epicocum nigrum was isolated and identified from E. milii leaves; other endophytic fungi were recovered and recognised morphologically from E. hirta leaves. The endophytic isolates were examined for their antifungal activity against F. solani on PDA plates using the dual culture technique, which exhibited a variable growth inhibition percentage. E. nigrum showed a highly inhibited percentage (94%) after 5 days. As well, E. nigrum metabolites were extracted by different solvents (e.g., ethyl acetate, dichloromethane, and chloroform: methanol, 2:1), and the crude extracts were active against F. solani compared to the control. The antifusarial activity was mostly reported in the ethyl acetate extract. Both light microscopy and scanning electron microscopy showed that higher concentrations of ethyl acetate extract caused a change in hyphal aggregation and spore formation in F. solani. Identification of antifungal components in ethyl acetate extract using GC-MS analysis revealed the presence of 2, 2, 3, 3, 4, 4-Hexadeutero Octadecanal, 2, 2-Dideutero Octadecanal, and Isochiapin B as major components that could have antifungal and antimicrobial substances. These results suggest using E. nigrum extracts for controlling phytopathogenic F. solani.

Phytochemical profiling and evaluation of antimicrobial activities of common culinary spices: Syzygium aromaticum (clove) and Piper nigrum (black pepper).

The increasing resistance of microbial pathogens to conventional antibiotics necessitates the exploration of alternative antimicrobial agents. This study aims to evaluate the antimicrobial potential and phytochemical properties of Syzygium aromaticum (clove) and Piper nigrum (black pepper) extracts, both of which are known for their historical use in traditional medicine and culinary applications. Hydroalcoholic and aqueous extracts of clove and black pepper were prepared. The antimicrobial activity of these extracts was assessed using the disk diffusion method against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and Aspergillus niger. Minimum inhibitory concentration (MIC) was determined using the broth dilution method. Qualitative phytochemical screening identified the presence of key bioactive compounds, while quantitative analysis measured total phenolic and flavonoid contents. LC-HRMS/MS analysis of ethanolic extracts was performed. Both spices extracts exhibited significant antimicrobial activity, with inhibition zones ranging from 14 to 18 mm. clove showed superior antimicrobial efficacy compared to black paper, particularly against fungi. MIC values ranged between 3 mg/mL and 6 mg/mL for both spices. Phytochemical analysis revealed higher total phenolic and flavonoid contents in clove, with hydroalcoholic extracts showing greater concentrations than aqueous extracts. HPLC quantified higher eugenol content in clove extracts and higher piperine content in black pepper extracts. The differences in bioactive compound content were statistically significant (p < 0.05). The study confirms that both spices possess significant antimicrobial properties, attributable to their rich phytochemical composition, particularly phenolics and flavonoids. Clove exhibited slightly superior antimicrobial activity compared to black paper. These findings support the potential use of these spices as complementary antimicrobial agents. Further research should investigate their synergistic effects with conventional antibiotics and explore their applications in food preservation and alternative medicine.

Antigenotoxic and cosmetic potential of elderberry (Sambucus nigra) extract: protection against oxidative DNA damage.

The integrity of the genome is under constant threat from both endogenous and exogenous factors that induce oxidative stress and accelerate ageing. The demand for natural and organic cosmetics is rising due to the harmful effects of synthetic genotoxic agents on human health and the environment. Elderberry (Sambucus nigra L.), a fruit rich in bioactive compounds such as polyphenols, has demonstrated significant antioxidant properties. This study aimed to evaluate elderberry extract's chemical characterization and biological activities in peripheral blood mononuclear cells exposed to streptonigrin and H2O2, both known for causing DNA damage. The antigenotoxic evaluation and antioxidant assays (ABTS and DPPH) were conducted to assess its biological properties. Using the Comet assay enhanced with formamidopyrimidine-DNA glycosylase (Fpg) to detect oxidized purines, we found that elderberry extract significantly reduced DNA damage. These findings suggest that elderberry has potential as a natural alternative to synthetic ingredients in cosmetics, offering protective benefits against DNA damage and contributing to anti-ageing and skin health.

Effect of Dietary Solanum nigrum Extract Supplementation on Growth Performance, Nutrient Digestibility, and Anti-Oxidant Activity in Labeo rohita Fingerlings

Effect of Dietary Solanum nigrum Extract Supplementation on Growth Performance, Nutrient Digestibility, and Anti-Oxidant Activity in Labeo rohita Fingerlings

Medicinal Importance of Solanum nigrum Linn; A Review

Solanum nigrum (Black nightshade) is an indigenous medicinal plant that has been utilized in traditional medical systems for ages. S. nigrum includes bioactive substances such as alkaloids, flavonoids, steroids, and saponins, according to phytochemical research. Extracts and isolated chemicals indicate antioxidant, anti-inflammatory, antibacterial, antifungal, anti-diabetic, anti-cancer, immune-stimulating, therapeutic, and heart-protective effects. S. nigrum extracts, in particular, limit cancer cell growth, minimize chemically induced organ damage, alter immunological function, and provide protection against oxidative stress, according to animal and cell culture studies. S. nigrum has traditionally been used for the prevention of symptoms such as discomfort, inflammation, infections, and problems with the gastrointestinal tract. Overall, this comprehensive study reveals the varied therapeutic qualities of S. nigrum that demand further exploration.

Antioxidant and Antihyperlipidemic Effect of Solanum Nigrum Extract in Experimental Diabetes Model

Diabetes mellitus (DM) is a chronic metabolic non-commu¬nicable disease; it is globally considered the fifth cause of death and it has attained worldwide epidemic pro¬portions. In our study, we aimed to investigate the diabetic effects of Solanum nigrum extract using the control group (C), diabetes group (D), groups given the Solanum nigrum extract (SN) and diabetes group + Solanum nigrum extract (D+SN). Our results observed the biological effectiveness of Solanum nigrum extract on glucose levels, significant increase serum glucose level group (D) (663±21.8 mg/dL) in comparison with C (131±9.8 mg/dL) were recorded. However, there were no significant difference in glucose level between C group (131±9.8 mg/dl) and SN group (196.14±12.1 mg/dL). Moreover, glucose level of D+SN group (484.8±40.0 mg/dL) was significantly higher than C (131±9.8 mg/dl), D (663±21.8 mg/dl) and SN groups (196.14±12.1 mg/dL). Total antioxidant status (TAS) level in D group (1.85±0.15.7) was significant when compared C group (1.28±0.17). Significant differences were observed between D group and D+SN group (1.54±0.07). However, TAS levels showed no significant difference in both SN (1.27±0.10) and D+SN (1.54±0.07) groups in comparison to the control group. Total oxidant status (TOS) level in D group (6.30±1.41) was given significant differences in comparison with control C (3.87±0.34), SN (4.87±0.80) group and D+SN (4.14±0.34) groups. In contrary, there were no significant differences between all of C, SN, D+SN groups. As a result, we can say that the Solanum nigrum plant extract is effective on diabetes, but it cannot lower the glucose level to normal levels, it needs to be investigated in future studies and its effects at different doses by different extraction methods.

Catechin as Natural Product Antifouling Agent against Biofilm Forming Bacteria from Visakhapatnam Coast, Andhra Pradesh, India

The aim of this work was to evaluate the effectiveness of Catechin as natural product antifoulants (NPAs) by inhibiting bacterial growth. 10 different medicinal plants namely Pongamia pinnata, Mucuna pruriens, Garcinia cambogia, Acacia catechu, Curcuma longa, Curcuma xanthorrhiza, Coffea arabica, Griffonia simplicifolia, Bacopa monnieri and Piper nigrum extracts were evaluated for potential natural product antifouling agent. Of which Acacia catechu and Curcuma longa showed best results, a significant zone of inhibition of bacteria was achieved by aqueous catechin. The secondary metabolite catechin is obtained by performing TLC, further purified by HPLC. Acacia catechu has antibacterial activity even up to 50mg L-1. The proposed partial structure of Catechin a secondary metabolite (flavanoid) is obtained by the results of Nuclear Magnetic Resonance, Mass Spectra and IR Spectra.