Purpose: This study aimed to determine the differences in the effects of high and low maximal oxygen consumption on the increase in lactic acid after night training. Materials and Methods: This type of research is comparative. This study uses the Bleep Test tool. The minimum total sample to be selected is 20 people in 2 groups. Data analysis in this study used a test for normality of data using the Shapiro-Wilk test. Test the homogeneity of the data using the chi-square test. Hypothesis testing using ANOVA with a significant level of 5%. Results: According to the results of the study, which showed that high and low levels of maximal oxygen consumption can affect the change or decrease of lactic acid levels, this can be confirmed by a significant value = 0.041 <0.05. Therefore, the hypothesis Ho is rejected and Ha is accepted, which means that there is a significant difference in the effect of high and low levels of maximal oxygen consumption on the reduction of lactic acid levels. Further analysis revealed that the exposure of the high maximal oxygen consumption group caused a greater decrease in lactic acid compared to the low maximal oxygen consumption group, with the high maximal oxygen consumption group having a mean of 6.370, while the low maximal oxygen consumption group low peak oxygen consumption averaged 5.460. Conclusions: There are differences in the effects of high and low levels of maximal oxygen consumption on lactic acid levels after nighttime exercise. The high-capacity effect of maximal oxygen uptake has a greater reduction in lactic acid levels compared to the low maximal oxygen uptake treatment.