Knowledge is crucial for improving animal welfare. People's perceptions, attitudes, and understanding may be a hindrance to adopting animal welfare-improving ideas which is crucial for developing effective animal welfare policies and advocacy in Nigeria. This study evaluates the current knowledge, attitudes, and perceptions of animal welfare among Nigerian residents. A cross-sectional survey using a structured questionnaire distributed via emails, social media platforms, and by personal contact. The survey collected data from 694 respondents across the 36 states and the Federal Capital Territory of Nigeria over 6 months (November 2023 and June 2024). Descriptive statistics, reliability testing, and inferential statistical tests were performed using SPSS for windows. The result revealed that majority (60-87.3%) of the respondents had a good knowledge of what constitutes cruelty to animals. Also, 88.8% were aware that animals have right to freedoms that could enhance their welfare, and 96.1% agreed that freedom from hunger and thirst is reasonable for animals. The standard deviations were relatively low, suggesting consistent responses among participants. Respondents (87.3%) also supported the enforcement of animal cruelty laws and the enactment of comprehensive animal welfare laws in Nigeria. The high knowledge scores among Nigerian residents suggest a strong foundational understanding of animal welfare principles. It is recommended that despite the result continual advocacy and policy development are still necessary to shape the public attitudes, and perceptions to animal.