Fungi belonging to the genus <em>Tuber</em> establish ectomycorrhizal symbioses with shrubs, trees and some herbaceous plants. Some <em>Tuber</em> species, for example, <em>T. melanosporum</em>, <em>T. magnatum</em>, <em>T. aestivum</em> are economically important because they produce edible fruiting bodies with a distinctive taste and flavor. Our concept of truffle ecophysiology is dominated by the symbiosis with deciduous hosts, such as: <em>Quercus</em> spp., <em>Fagus sylvatica</em>, <em>Castanea sativa</em>, <em>Corylus</em> spp., <em>Carpinus betulus</em>, <em>Ostrya carpinifolia</em>, <em>Betula verrucosa</em>, and <em>Tilia</em> spp., whereas the real range of hosts in nature seems to be much wider. Moreover, interactions between <em>Tuber</em> mycelium and plant community could be more complex than just forming the ectomycorrhizal symbiosis. Here we show our inventory of plants and soils at six truffle’ sites in the southern part of Poland (Nida Basin and Przedbórz Upland). The aim of this study was to widen our understanding of ecological factors affecting <em>Tuber</em> spp., in the context of pioneering stage of research on truffles in Poland. We hope our findings will have a practical application and will help to choose suitable soils for truffle orchards.