Nibea Coibor Research Articles

Cloning, tissue distribution, functional characterization and nutritional regulation of Δ6 fatty acyl desaturase in chu's croaker Nibea coibor

In the present study, we successfully cloned the full-length cDNA of a Δ6 fatty acyl desaturase (Δ6 Fad) of chu's croaker Nibea coibor. The cloned cDNA sequence was 1590bp at full length, including a 5′-UTR of 137bp, a 3′-UTR of 115bp and a mean ORF of 1338bp that specified a peptide of 445 aa. Sequence analysis of the deduced polypeptide showed that it contained all the characteristics of ∆6 Fad identified such as one cytochrome b5 domain, three trans-membrane regions and three histidine-rich boxes. Tissue distribution revealed that it strongly expressed in brain, significantly high than the level in eye, spleen, intestine, heart, gill, liver, stomach, muscle, liver and kidney. Functional characterization by heterologous expression in yeast showed that the chu's croaker desaturase was a Fad2 with ∆6 Fad activity, with 9.36% conversion of 18:2n-6 to 18:3n-6 and 4.93% of 18:3n-3 to 18:4n-3, respectively. Nutritional study showed that the expression of ∆6 Fad in liver were not significantly affected among treatments.

Effects of Dietary Fish Oil Replacement with Palm Oil on the Growth, Feed Utilization, Biochemical Composition, and Antioxidant Status of Juvenile Chu's Croaker, Nibea coibor

AbstractA 56‐d feeding trial was conducted to evaluate the growth performance, feed utilization, biochemical composition, and antioxidant status of chu's croaker, Nibea coibor (12.6 ± 0.1 g) in net pens fed diets supplemented with various levels of crude palm oil: 0% fish oil (FO), 20% palm oil (2PO), 40% (4PO), 60% (6PO), 80% (8PO), and 100% (10PO). Weight gain and specific growth rate (SGR) were significantly lower in fish fed 8PO and 10PO diets. The increase of PO levels did not affect fish survival, feed intake, and feed conversion ratio. Feeding diet 10PO led to significantly higher lipid content in the liver, but not the whole body and dorsal muscle, than diets FO and 2PO. PO supplementation led to increased α‐tocopherol, but decreased thiobarbituric acid‐reactive substances, n‐3 highly unsaturated fatty acid (n‐3 HUFA), and n‐3/n‐6 fatty acid ratio, in both the liver and muscle. Hepatic superoxide dismutase and catalase were significantly higher in fish fed 10PO diet than FO diet. Polynomial regression analysis showed that the maximum SGR was obtained at 24.2% crude PO. Results of this study suggested that PO could replace up to 60% of FO without adverse effect on growth performance or nutritional quality of fish.

Effects of protein levels on growth, feed utilization, body composition, amino acid composition and physiology indices of juvenile chu's croaker,Nibea coibor

A 49-day feeding trial was conducted to determine the optimal dietary protein level for juvenile chu's croaker Nibea coibor in sea cages. Five isocaloric diets were formulated to contain graded levels of protein: 360 (D36), 400 (D40), 440 (D44), 480 (D48) and 520 (D52) g kg−1 diet. Each diet was randomly assigned to triplicate groups of 25 fish (mean 76.0 ± 0.8 g) in a total of 15 floating cages. Specific growth rate (SGR), protein retention efficiency (PRE), feed efficiency and feed intake were significantly higher at D44 and D48. Consumed protein (CP) obtained the maximum at D48, and protein efficiency ratio was significantly higher in fish fed with D44 and D48 compared with fish fed with D36 and D52. Hepatosomatic index and condition factor were not affected. Body protein and moisture content increased with increasing dietary protein, while lipid and ash content were not affected. Individual and total essential amino acids maximized at D44. Digestive enzymes and physiology indices in intestine, liver and plasma were differentially affected. Based on polynomial regression analysis of SGR, PRE and CP, we suggested that the optimum dietary protein level for juvenile chu's croaker was 444–478 g kg−1 diet.

Effects of dietary lipid levels on growth, feed utilization, body composition, fatty acid profiles and antioxidant parameters of juvenile chu’s croaker Nibea coibor

The present study aimed at determining the growth performance, feed utilization, body composition and antioxidant status of chu’s croaker (Nibea coibor) juveniles fed with increasing levels of dietary lipid: 6 % (D6), 9 % (D9), 12 % (D12) and 15 % (D15). Each diet was assigned to triplicate groups of fish in a total of 12 floating pens (300 fish, 25 fish per pen). After a 49-day growth trial, survival rate was not affected. D12 and D15 led to significantly higher specific growth rate (SGR). Fish fed D12 showed the highest protein efficiency ratio (PER), protein and lipid retention efficiencies (PER and LRE, respectively). The hepatosomatic and viscerosomatic indexes (HIS and VSI, respectively) increased, while feed conversion ratio (FCR) and feed intake (FI) decreased. Body protein, ash and muscle lipid contents were not significantly affected, but significantly higher body and liver lipid were noticed in fish fed with D15. Monounsaturated fatty acid (MUFA) was found to decrease compared to the experimental diets in muscle, while high unsaturated fatty acid (HUFA) was selectively accumulated in all treatments. Serum alanine transaminase (ALT, 14.13–22.53 U/L), aspartate transaminase (AST, 34.31–51.25 U/L), cholesterol (CHO, 2.02–3.03 mmol/L) and triglyceride (TG, 5.61–8.50 mmol/L) were correlated with increasing dietary lipids. Liver malate dehydrogenase (MDA, 3.32–6.67 mmol/L) and superoxide dismutase (SOD, 42.69–52.86 U/mg prot) increased with dietary lipids, while total antioxidant capacity (t-AOC, 1.08–3.50 U/mg prot) decreased. Polynomial regression analyses between SGR and dietary lipid levels showed that the optimal dietary lipid requirement of chu’s croaker is 12.9 % of dry matter.

The complete mitochondrial genome of Nibea coibor (Perciformes: Sciaenidae)

In this study, the complete mitochondrial genome (mitogenome) sequence of Nibea coibor has been determined by long polymerase chain reaction and primer walking methods. The complete mitochondrial genome is 16,509 bp in length and contains 37 mitochondrial genes (13 protein-coding genes, 2 ribosomal RNA (rRNA), 22 transfer RNA (tRNA)) and a control region as other bony fishes. Within the control region, we identified the termination-associated sequence domain (TAS), the central conserved sequence block domains (CSB-F, CSB-E, CSB-D and CSB-C), and the conserved sequence block domains (CSB-2, CSB-3).

The complete mitochondrial genome of Nibea coibor (Perciformes, Sciaenidae)

We present the complete mitochondrial genome of Nibea coibor in this study. The mitochondrial genome is 16,502 bp in length and consists of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region. All of them are encoded on the heavy strand except ND6 and 8 tRNA genes on the light strand. The nucleotide compositions of the light strand were 31.62% of G, 26.88% of T, 25.19% of A and 16.31% of C. Three types of initiation codons are ATA (ATP6), ATG (ND2, COXI, COXII, ATP8, COXIII, ND3, ND4L, ND4, ND5, ND6, Cytb) and GTG (ND1), and 3 types of termination codons are AGA (COXI), T (ND2, COXII, ND3, ND4) and TAA (ATP6, ATP8, COXIII, ND4L, ND5, ND6, Cytb, ND1). There are 13 intergenic spacers, 7 gene overlaps and no tandem repeat sequence. Gene arrangement and distribution are consistent with the typical vertebrates.

Nibea coibor growth hormone gene: Its phylogenetic significance, microsatellite variation and expression analysis

The growth hormone (GH) gene has been characterized for a number of fishes and used to establish phylogenetic relationships and as a candidate gene for studies of genetic variation in connection with growth traits. In this study, we report the genomic structure of Nibea coibor GH (designated as ncGH) including its 5’-flanking region, being cloned by homology-cloning and chromosome walking methods. The ncGH gene spans approximately 3.0 kb and consists of six exons and five introns, as found for all cloned teleost GH genes with the exception of carps and catfish. The 5’-flanking region contains consensus sequences for a TATA box, a CRE, a pit-1α, a TRE, two HNF-3, a ERE and a GRE. Five microsatellites are identified in the ncGH gene and three of them are polymorphic marker. The open reading frame (ORF) of ncGH is 615 bp in length encoding a polypeptide of 204 amino acids with an estimated molecular mass of 23.04 kDa and theoretical isoelectric point of 7.79. The precursor of ncGH consists of a 17 amino-acid signal peptide and a 187 amino-acid mature peptide. The four Cys residues are located at conserved positions (Cys 69, Cys 177, Cys 194 and Cys 202), and One possible site for N-glycosylation (Asn-X-Ser/Thr motif) is present at Asn 201. The coding region sequence of ncGH is used to align with the sequences of 18 other species from Percoidei and one species from Anabantoidei using Clustal X. A matrix of 612 bp was used to construct the phylogenetic trees using neighbor-joining and maximum parsimony methods. The phylogenetic trees by two methods are identical in most of the clades with high bootstrap support. Every family all forms independent monophyly on the phylogenetic trees, in the family, the different species also forms the monophyly according to the different genera. The results are also identical to those from morphological data, and demonstrated that the GH gene is very suitable for phylogenetic relationship analysis of Percoidei. To validate the predicted exon/intron boundaries, ncGH cDNA is cloned using RT-PCR, and tissue distributions are investigated using semi-quantitative RT-PCR method. The results indicate that the predicted exon/intron is correct, the ncGH mRNA are mainly expressed in pituitary, and weakly expressed in ovary, brain, liver, gill, intestine, muscle and hear, but not expressed in spleen and kidney.

The syntypes of the sciaenid Corvina albida Cuvier and the status of Dendrophysa hooghliensis Sinha and Rao and Nibea coibor (nec Hamilton) of Chu, Lo & Wu

Day (1876) rightly used the name Corvina albida Cuvier for a species with a pair of one on either side of the median pore of the lower jaw, and Dendrophysa hooghliensis Sinha & Rao (1969) is a synonym of it. Following Talwar (1970c), it may be known as Daysciaena albida. Reports of ‘Pseudosciaenu’ albida or Nibea cuibor from the western Pacific are of this species, but at least some refer to a Nibea, here renamed Nibea chui. The genus is redefined to exclude species without a cephalic extension to the swim‐bladder and a generic name is proposed for ‘Nibea’ diacanthus (Lacépède). A key to the species of is given.