New Plant Type Research Articles

Korelasi dan Sidik Lintas Karakter Agronomi Padi Hibrida

Two approachment to increase yield productivity with new variety throught new plant type (NPT) concept and heterosis exploitation of hybrid rice. The research was to evaluated yield and yield component of 36 hybrid rice genotypes in preliminary yield trial at two locations. The experiment was conducted in the second season MT (II) 2013 at the Sukamandi experimentalstation, Subang, West JavaProvince and in Batang, Central Java Province by usingRandomized Complete Block Design (RCBD). The material used were36hybrid rice genotypes and four check varieties namely Hipa8, Hipa Jatim2,Ciherang, and Longping Optima. The results showed that grain yield had been effected by location, genotypes, and both interactions. There were two hybrid rice genotypes that give high yield potential than four check varieties, and one hybrid rice genotype significantly different from Longping Optima. Those hybrids were GMJ6/CRS519 (9.9 t/ha) and GMJ14/CRS757 (9.5 t/ha), and A1/CRS518 (6.2 t/ha). Based on path analysisnumber of productive tiller and number of total grain per panicle have direct effect to yield with path coefficient as 0.4028 and 0.2153. Length panicle and seed set also gave positif and significant direct effect to yield as 0.095 and 0.0956, so these characteristics could be used as selection criteria.

Genetic characterization of introgression lines with the genetic background of the Indica-type rice (Oryza sativa L.) cultivar IR 64 under irrigated lowland and upland conditions

We characterized 333 introgression lines (INLs) harboring chromosome segments from new plant type (NTP) and Japonica-type cultivars with the genetic background of the elite Indica-type rice (Oryza sativa L.) cultivar IR 64, by evaluating yield components and dry matter production under irrigated lowland (IL) and upland (UP) culture in 2011 and 2012; and elucidated the relationships between the traits and chromosome segments introduced from the donors. The panicle weight (PW), PW/total plant weight (PW/TW), number of fertile spikelets (FS), and FS/total number of spikelets per panicle (FS/TS) of the INLs increased, while the culm weight (CW) and panicle number (PN) decreased. Most INLs produced more dry matter in the UP than in the IL culture. The INLs were clustered into three groups (A–C) according to their traits in the UP culture, and four groups (I–IV) according to their traits in the IL culture. They were then sorted into six groups (A-I, A-II, B-II, B-III, C-III, and C-IV) according to the relationships between culture conditions. The INLs of group A-II had increased panicle length (PL), PW, PW/TW, FS, and TS, and these traits were stable in both IL and UP culture. In the other groups, the trait values changed with the conditions and season. Association analyses using trait phenotype and genotype data of SSR markers detected 166 quantitative trait loci (QTLs) for 10 traits, distributed on all chromosomes except 3 and 10. Of these, 81 QTLs for DH, CL, PL, CW, PW, PW/TW, PN, FS, TS, and FS/TS were newly detected. The INLs were characterized under different conditions, and dramatic trait modifications in IR64 rice were associated with these QTLs, through introgression from the donor cultivars. Thus, these INLs and QTLs will be useful in genetic studies to identify the gene(s) that modified IR64, and as breeding materials for genetic improvement of Indica-type cultivars.

Identification of a low tiller gene from a new plant type cultivar in rice (Oryza sativa L.).

We characterized a rice introgression line, YTH34, harboring a chromosome segment from a New Plant Type (NPT) cultivar, IR65600-87-2-2-3, in the genetic background of an Indica Group elite rice cultivar, IR 64, under upland and irrigated lowland conditions in Japan. The number of panicles (as an indicator of tiller number) and number of spikelets per panicle of YTH34 were lower than those of IR 64 under irrigated lowland conditions, but both of those as well as culm length, panicle length, seed fertility, panicle weight, whole plant weight, and harvest index were dramatically reduced under upland conditions. And the low tiller of YTH34 was confirmed to start after the maximum tiller stage. In particular, the decrease of panicle number was remarkable in upland, so we tried to identify the chromosome location of the relevant gene. Through segregation and linkage analyses using F3 family lines derived from a cross between IR 64 and YTH34, and SSR markers, we found that low tiller number was controlled by a single recessive gene, ltn2, and mapped with the distance of 2.1 cM from SSR marker RM21950, in an introgressed segment on chromosome 7. YTH34 harboring ltn2 and the genetic information for DNA markers linked will be useful for genetic modification of plant architectures of Indica Group rice cultivar.

Quantitative trait locus analysis and fine mapping of the qPL6 locus for panicle length in rice.

Two QTLs were identified to control panicle length in rice backcross lines, and one QTL qPL6 was finely mapped with potential in high yield breeding. Panicle length (PL) is the key determinant of panicle architecture in rice, and strongly affects yield components, such as grain number per panicle. However, this trait has not been well studied genetically nor its contribution to yield improvement. In this study, we performed quantitative trait locus (QTL) analysis for PL in four backcross populations derived from the cross of Nipponbare (japonica) and WS3 (indica), a new plant type (NPT) variety. Two QTLs were identified on chromosome 6 and 8, designated as qPL6 and qPL8, respectively. Near-isogenic lines (NILs) were developed to evaluate their contribution to important agronomic traits. We found that qPL6 and qPL8 had additive effects on PL trait. For the qPL6 locus, the WS3 allele also increased panicle primary and secondary branches and grain number per panicle. Moreover, this allele conferred wide and strong culms, a character of lodging resistance. By analyzing key recombinants in two steps, the qPL6 locus was finely mapped to a 25-kb interval, and 3 candidate genes were identified. According to the single nucleotide polymorphisms (SNPs) within candidate genes, 5 dCaps markers were designed and used to get haplotypes of 96 modern Chinese varieties, which proved that qPL6 locus is differentiated between indica and temperate japonica varieties. Taken together, the superior qPL6 allele can be applied in rice breeding programs for large sink size, particularly for japonica varieties that originally lack the allele.

Transcriptomic analysis reveals differential gene expressions for cell growth and functional secondary metabolites in induced autotetraploid of Chinese woad (Isatis indigotica Fort.).

The giant organs and enhanced concentrations of secondary metabolites realized by autopolyploidy are attractive for breeding the respective medicinal and agricultural plants and studying the genetic mechanisms. The traditional medicinal plant Chinese woad (Isatis indigotica Fort., 2n = 2x = 14) is now still largely used for the diseases caused by bacteria and viruses in China. In this study, its autopolyploids (3x, 4x) were produced and characterized together with the 2x donor for their phenotype and transcriptomic alterations by using high-throughput RNA sequencing. With the increase of genome dosage, the giantism in cells and organs was obvious and the photosynthetic rate was higher. The 4x plants showed predominantly the normal meiotic chromosome pairing (bivalents and quadrivalents) and equal segregation and then produced the majority of 4x progeny. The total 70136 All-unigenes were de novo assembled, and 56,482 (80.53%) unigenes were annotated based on BLASTx searches of the public databases. From pair-wise comparisons between transcriptomic data of 2x, 3x, 4x plants, 1856 (2.65%)(2x vs 4x), 693(0.98%)(2x vs 3x), 1045(1.48%)(3x vs 4x) unigenes were detected to differentially expressed genes (DEGs), including both up- and down-regulated ones. These DEGs were mainly involved in cell growth (synthesis of expansin and pectin), cell wall organization, secondary metabolite biosynthesis, response to stress and photosynthetic pathways. The up-regulation of some DEGs for metabolic pathways of functional compounds in the induced autotetraploids substantiates the promising new type of this medicinal plant with the increased biomass and targeted metabolites.

Changes in the Chlorophyll Content and Cytokinin Levels in the Top Three Leaves of New Plant Type Rice During Grain Filling

This paper reports the ways that the differences in leaf senescence are related to grain filling, grain yield, and the dynamics of cytokinins (CKs) in the top three leaves of four field-grown new plant type (NPT) rice, a tropical japonica developed at the International Rice Research Institute, Philippines, to increase the yield potential of rice. The chlorophyll content in leaves decreased from flowering to maturity in all the NPT lines, whereas the grain filling percentage was higher in the fast-senescing NPT line than in slow-senescing NPT line. Grain yield was positively correlated with senescence in the flag leaf. Rapid changes in the CK levels were recorded in the leaves of the fast-senescing line, whereas the CK levels were relatively stable in leaves of the slow-senescing line, suggesting that the dynamics of CKs in the fast-senescing line are vital for fast senescence. There were no significant changes in bioactive CKs, CK O-glucosides (storage CKs), and cis-zeatin derivatives in different leaves of the slow-senescing NPT line between 0 and 3 weeks after flowering, suggesting that the content of these CKs is relatively stable during grain filling. A progressive increase in levels of bioactive CKs was positively correlated with gradual accumulation of CK N-glucosides (inactive CKs) in the top three leaves of the slow-senescing NPT line, whereas the decrease of bioactive CKs in the flag leaf of the fast-senescing line was accompanied by accumulation of CK O-glucosides. These results suggest that there is a higher rate of biosynthesis and/or import of bioactive CKs as well as their turnover which may favor delay of leaf senescence in the slow-senescing NPT line.

Study of Micro Hydropower Plant Operating in Gravitational Vortex Flow Mode

This paper refers to experimental study of a new type of micro hydropower plant for energy conversion of low river water heads into mechanical work by imposing gravitational vortex flow in spiral form through a vertical conical channel. In this vortex, at different heights of the conical channel, are positioned rotor blades of the turbine with different values of specific speed. The characteristics of the gravitational vortex of water flow through the conical channel, the speed and the power characteristics of the micro hydropower plant were investigated and attempts to efficiency modeling were performed.

Biosafety considerations of RNAi-mediated virus resistance in fruit-tree cultivars and in rootstock

A major application of RNA interference (RNAi) is envisaged for the production of virus-resistant transgenic plants. For fruit trees, this remains the most, if not the only, viable option for the control of plant viral disease outbreaks in cultivated orchards, due to the difficulties associated with the use of traditional and conventional disease-control measures. The use of RNAi might provide an additional benefit for woody crops if silenced rootstock can efficiently transmit the silencing signal to non-transformed scions, as has already been demonstrated in herbaceous plants. This would provide a great opportunity to produce non-transgenic fruit from transgenic rootstock. In this review, we scrutinise some of the concerns that might arise with the use of RNAi for engineering virus-resistant plants, and we speculate that this virus resistance has fewer biosafety concerns. This is mainly because RNAi-eliciting constructs only express small RNA molecules rather than proteins, and because this technology can be applied using plant rootstock that can confer virus resistance to the scion, leaving the scion untransformed. We discuss the main biosafety concerns related to the release of new types of virus-resistant plants and the risk assessment approaches in the application of existing regulatory systems (in particular, those of the European Union, the USA, and Canada) for the evaluation and approval of RNAi-mediated virus-resistant plants, either as transgenic varieties or as plant virus resistance induced by transgenic rootstock.

A Trend in Electrochemical Plants: Electrical Issues with Direct to Bleach

This article presents the challenges faced and lessons learned by the scaling down of processes and equipment in the design and construction of direct to bleach plants and also examines some electrical issues with a new type of electrochemical process plant.

Molten-Salt Power Towers: Newly Commercial Concentrating Solar Storage

Molten-salt storage is already commercially available for concentrating solar power (CSP) plants, allowing solar power to be produced on demand and to “backup” variable renewable sources such as wind and photovoltaics. The first CSP plants to operate commercially with molten-salt storage utilized parabolic trough concentrators, for example, the Andasol-1 plant. A new type of storage plant has now reached commercial status, with the 19.9-MWe Torresol Gemasolar power tower, featuring 15 h of molten-salt storage, having come online in Spain in May 2011. Advantages of the power tower storage system include the elimination of heat transfer oil and associated heat exchangers, a lower salt requirement, higher steam cycle efficiency, better compatibility with air cooling, improved winter performance, and simplified piping schemes. Near-term advances in molten-salt power tower technology include planned up-scaling, with SolarReserve due to begin constructing a 110-MWe plant in Nevada by August 2011. Other advances include improvements to the thermal properties of molten salts and the development of storage solutions in a single tank. With these developments at hand, CSP will continue to provide dispatchable solar power, with the capacity to provide energy storage for 100% renewable electricity grids in sun-belt countries.

Theoretical settings of photovoltaic-hydro energy system for sustainable energy production

Since the main problem of continuous energy supply from photovoltaic (PV) power plant is intermittence and inability to provide continuous energy supply, this paper proposes its hybridization with hydro energy, or with pump storage hydroelectric (PSH) as a possible solution. This creates a new type of sustainable hybrid power plant which can work continuously, using solar energy as primary energy source and water for energy storage. The characteristics of the solution as an open thermodynamic system are presented, as well as the basic theoretical settings for its application, i.e. key relationships between power and collector field area of PV power plant and working volume of upper storage. The paper introduces hydrological and hydro-energetic indicators for the hybrid plant description, “artificial rainfall”, as the relationship between the water pumped into the upper water storage of the PSH (artificial water inflow) and collector field area of the PV power plant, as well as hydroenergy potential. The proposed hybrid electric power plant does not emit greenhouse gases, produce waste or significantly exploit water resources while the risks to humans and the environment are far smaller than when using conventional technology. This solution is flexible for implementation and can be applied in various climates, hydrological and physical conditions. It is especially productive in cases of joint use of solar and hydro energy where they naturally complement each other as natural energy sources in the annual working cycle.

QTL analysis of novel genomic regions associated with yield and yield related traits in new plant type based recombinant inbred lines of rice (Oryza sativa L.)

BackgroundRice is staple food for more than half of the world’s population including two billion Asians, who obtain 60-70% of their energy intake from rice and its derivatives. To meet the growing demand from human population, rice varieties with higher yield potential and greater yield stability need to be developed. The favourable alleles for yield and yield contributing traits are distributed among two subspecies i.e., indica and japonica of cultivated rice (Oryza sativa L.). Identification of novel favourable alleles in indica/japonica will pave way to marker-assisted mobilization of these alleles in to a genetic background to break genetic barriers to yield.ResultsA new plant type (NPT) based mapping population of 310 recombinant inbred lines (RILs) was used to map novel genomic regions and QTL hotspots influencing yield and eleven yield component traits. We identified major quantitative trait loci (QTLs) for days to 50% flowering (R2 = 25%, LOD = 14.3), panicles per plant (R2 = 19%, LOD = 9.74), flag leaf length (R2 = 22%, LOD = 3.05), flag leaf width (R2 = 53%, LOD = 46.5), spikelets per panicle (R2 = 16%, LOD = 13.8), filled grains per panicle (R2 = 22%, LOD = 15.3), percent spikelet sterility (R2 = 18%, LOD = 14.24), thousand grain weight (R2 = 25%, LOD = 12.9) and spikelet setting density (R2 = 23%, LOD = 15) expressing over two or more locations by using composite interval mapping. The phenotypic variation (R2) ranged from 8 to 53% for eleven QTLs expressing across all three locations. 19 novel QTLs were contributed by the NPT parent, Pusa1266. 15 QTL hotpots on eight chromosomes were identified for the correlated traits. Six epistatic QTLs effecting five traits at two locations were identified. A marker interval (RM3276-RM5709) on chromosome 4 harboring major QTLs for four traits was identified.ConclusionsThe present study reveals that favourable alleles for yield and yield contributing traits were distributed among two subspecies of rice and QTLs were co-localized in different genomic regions. QTL hotspots will be useful for understanding the common genetic control mechanism of the co-localized traits and selection for beneficial allele at these loci will result in a cumulative increase in yield due to the integrative positive effect of various QTLs. The information generated in the present study will be useful to fine map and to identify the genes underlying major robust QTLs and to transfer all favourable QTLs to one genetic background to break genetic barriers to yield for sustained food security.

Characterization of near-isogenic lines carrying QTL for high spikelet number with the genetic background of an indica rice variety IR64 (Oryza sativa L.).

Total spikelet number per panicle (TSN) is one of the most important traits associated with rice yield potential. This trait was assessed in a set of 334 chromosomal segment introgression lines (ILs: BC3-derived lines), developed from new plant type (NPT) varieties as donor parents and having the genetic background of an indica-type rice variety IR64. Among the 334 ILs, five lines which had different donor parents and showed significantly higher TSN than IR64 were used for genetic analysis. Quantitative trait locus (QTL) analysis was conducted using F2 populations derived from crosses between IR64 and these ILs. As a result, a QTL for high TSN (one from each NPT donor variety) was detected on common region of the long arm of chromosome 4. The effect of the QTL was confirmed by an increase in TSN of five near-isogenic lines (NILs) developed in the present study. The variation in TSN was found among these NILs, attributing to the panicle architecture in the numbers of primary, secondary and tertiary branches. The NILs for TSN and the SSR markers linked to the TSN QTLs are expected to be useful materials for research and breeding to enhance the yield potential of rice varieties.

Window of opportunity – potential of increase in profitability using modular compact plants and micro-reactor based flow processing

Recently, much focus has been given to a new type of chemical production plant, with the aim of a much faster time-to-market (“50% idea”) and better cash-flow revenue. The main enabling technology is to have the plants pre-manufactured and assembled by a modular construction and to use innovative, smart-scale processing and apparatus technology, in order to achieve a compact overall plant footprint. Focal points in such technology are on the one hand, flow processing, with micro process technology as a cutting-edge cornerstone, and on the other hand, the container framework. Yet, other process-intensified technologies are suitable as well such as milli-flow or upgraded batch technologies. Finally, process robustness and short-time applicability make the decision. In this paper, for the first time, a CAPEX and OPEX analysis of the new plant technology is done, at the example of the Evotrainer production platform. This platform is pre-manufactured in serial and provides all the utilities needed around the reactor and e.g., separator to be tailored and inserted. The unit-operational modularization, with respective definition of interfaces, was developed further to a so-called functional modularization, where different cabinets with pre-defined functions and equipment are proposed. Three virtual microreactor applications were used and stand as model-based scenarios for market applications in bulk chemistry, fine chemistry and pharmacy. It is shown, in many facets, that the Evotrainer infrastructure based plants indeed have a faster payback and higher earnings as compared to conventional technology, particularly when serving high-priced markets such as pharmaceutical applications. Further, the combination with novel chemical routes or novel processing (Novel Process Windows) is advantageous. Micro process technology is one of the key enablers and was taken here, since the dataset of such technology was readily available to the authors due to past research efforts and there is some general belief in the combination to the so-called “Future Factories”. Yet, it stands also for any other process intensification technology which can achieve the same performance level and which is able to satisfy the needs of a producing industry.

PERFORMANCE OF GRAIN QUALITY AND AROMA OF AROMATIC NEW PLANT TYPE PROMISING RICE LINES

Grain quality and aroma are important characteristics of rice that affect consumer acceptance. This research was conducted to study the grain quality and aroma of aromatic new plant type (NPT) promising rice lines. Thirty five lines as well as Ciherang and Sintanur varieties were planted at Bogor and Pusakanagara, West Java in the dry season (DS) 2009 and wet season (WS) 2009. Three methods, i.e. leaf aroma tested with KOH, rice aroma tested in the test tube, and cooked rice aroma test, were used to evaluate the aroma of the lines. The results showed that line B11742-RS*2-3-MR-34-1-2-1 was aromatic identified using different methods. The line had long, slender, and small chalkiness grains, high percentage of head rice, high amylose, and hard texture. Lines IPB 140-F-6, B11249-9C-PN-3-3-2-2-MR-1, and B11955-MR-84-1-4 also had a high aroma score and grain yield. Testing leaf aroma with KOH can be used as early selection method in breeding program for aromatic lines. Lines derived from aromatic parents from highlands of South Sulawesi did not show consistent aroma under three testing methods. Those tested lines had good grain quality, both physical and cooked rice quality.<br /><br /><br />

PERFORMANCE OF GRAIN QUALITY AND AROMA OF AROMATIC NEW PLANT TYPE PROMISING RICE LINES

Grain quality and aroma are important characteristics of rice that affect consumer acceptance. This research was conducted to study the grain quality and aroma of aromatic new plant type (NPT) promising rice lines. Thirty five lines as well as Ciherang and Sintanur varieties were planted at Bogor and Pusakanagara, West Java in the dry season (DS) 2009 and wet season (WS) 2009. Three methods, i.e. leaf aroma tested with KOH, rice aroma tested in the test tube, and cooked rice aroma test, were used to evaluate the aroma of the lines. The results showed that line B11742-RS*2-3-MR-34-1-2-1 was aromatic identified using different methods. The line had long, slender, and small chalkiness grains, high percentage of head rice, high amylose, and hard texture. Lines IPB 140-F-6, B11249-9C-PN-3-3-2-2-MR-1, and B11955-MR-84-1-4 also had a high aroma score and grain yield. Testing leaf aroma with KOH can be used as early selection method in breeding program for aromatic lines. Lines derived from aromatic parents from highlands of South Sulawesi did not show consistent aroma under three testing methods. Those tested lines had good grain quality, both physical and cooked rice quality.<br /><br /><br />

Study on Freeboard Properties to Maintain Low N2O Emissions from Sewage Sludge in a Fluidized Bed Combustor

A new type of incineration plant, which was equipped with a pressurized bubbling fluidized bed combustor coupled with a turbocharger, was proposed. N2O emissions in the proposed combustor of the demonstration plant were less than half of those observed in an atmospheric plant. In this study, the freeboard properties in the combustor were investigated to clarify the detailed reason for the low N2O emissions under pressurized conditions. First, the effect of a wide range of operating pressures on N2O emissions in a laboratory-scale pressurized fluidized bed combustor, which can control the freeboard temperature independently using an electric heater, was examined experimentally. Second, the effect of the operating pressure on the freeboard temperature profiles was estimated theoretically by a computational analysis. As a result, N2O emissions were obviously decreased with an increasing freeboard temperature even when the operating pressure changed substantially. Thus, the freeboard temperature is an importa...

Keragaan varietas dan galur padi tipe baru Indonesia dalam sistem ratun

Eighteen Indonesian new plant type (NPT) of rice varieties and lines were evaluated in a ratoon system. The results showed that ratooning ability was related to the vegetative growth prior to harvest, which gave high ability ratoon growth. New plant type varieties and several lines of rice had better vegetative growth and produced higher ratoon than other genotypes. Ratoons started to develop 2-7 days after harvest, with an average number of leaf 2-4 per tiller. Number of productive tillers of ratoon ranged from 6.0 to 30.0 per hill, with number of grain per panicle ranged from 38.0 to 228.2, and grain weight per hill of ratoon 10.4 to 31.2 g, dependent on genotypes. Maturity of ratoon was also dependent on genotypes. On average, maturity was 68 days after harvest of the main crop. Grouping of ratooning ability based on hierarchical cluster analysis resulted in three groups, namely nine genotypes had high potential, fi ve genotypes had medium potential, and four genotypes had low potential. This study indicated that ratoon is potential to increase rice planting index in Indonesia. The genotypes with high ratoon ability need to be evaluated for agronomic performance in the fi eld. Keywords: new plant type, ratooning ability, rice

Yield stability and adaptability of aromatic New Plant Type (NPT) rice lines

Aromatic new plant type (NPT) rice lines were selected to obtain high yielding and aromatic lines. The objectives of the research were to study the yield stability and adaptability of 35 NPT rice lines across different environment, with Ciherang and Sintanur as check varieties. The lines planted at two locations, Bogor and Pusakanagara in two seasons (2009 dry and wet seasons, DS-WS). The experiment used randomized complete block design (RCBD), with three replications. The 21-day-old seedlings were planted with spacing of 20 cm x 20 cm, with a plot size of 2 m x 5 m. Yield stability and adaptability were estimated by using coeffi cient regression (bi) and general mean of yield by Finlay-Wilkinson method. Combined analysis of variance showed that lines (G), environment (E), and the G x E interaction were signifi cantly different. Lines showed different stability and adaptability. Several lines yielded higher than Ciherang variety. Nine lines were classifi ed as stable and widely adapted at the marginal environment, i.e IPB 116-F-3-1, IPB 117-F-4-1, IPB-117-F-14-2, IPB-117-F-15-2, IPB-117F-17-5, IPB 140-F-1-1, 140-F-IPB, 2-1, IPB 140-F-3, and IPB 149-F-2. Lines IPB 113-F-2, IPB 140-F-4, IPB 140-F-6, IPB 140-F-7, and B11738-MR-Si-1-2-1-2 were not stable and adapted only in optimum environmental condition (bi > 1) while IPB 116-F-46-1, IPB-117-F 17-4, IPB-117-F 18-3 and B11955-MR-84-1-4 has the value of bi < 1 or adaptable to marginal environments.

Development of introgression lines of an Indica-type rice variety, IR64, for unique agronomic traits and detection of the responsible chromosomal regions

A total of 334 introgression lines (INLs: BC 3-derived lines) derived from crosses between a recurrent parent of Indica rice cultivar IR64 and 10 donor parents, including new plant type (NPT) lines IR65600-87-2-2-3, IR65598-112-2, IR65564-2-2-3, IR69093-41-2-3-2, IR69125-25-3-1-1, Hoshiaoba, IR66215-44-2-3, IR68522-10-2-2, IR71195-AC1, and IR66750-6-2-1, have been developed. These INLs with IR64 genetic background were characterized for eight agronomic traits: days to heading, culm length, leaf width, leaf length, panicle length, panicle number, 100-grain weight, and total spikelet number per panicle at the International Rice Research Institute from 2005 to 2007. To identify introgressed segments from the donor parents, genotypes of the 334 INLs were detected using more than 200 polymorphic simple sequence repeat markers. These segments detected on chromosomes 1, 2, 4, 5, and 6 were commonly introgressed across the INLs from more than four donor varieties. Based on the data of phenotype and genotype for the 334 INLs, associations between agronomic traits and introgressed chromosomal segments in the 334 INLs were investigated. A total of 54 regions for the eight traits were detected: seven regions for days to heading, eight regions for culm length, eight regions for leaf width, four regions for leaf length, six regions for panicle length, three regions for panicle number per plant, seven regions for 100-grain weight, and 11 regions for total spikelet number per panicle. Among them, the region on the long arm of chromosome 4 was associated with characteristics of the NPT such as long leaf, broad leaf, and high spikelet number. The developed 334 INLs with the IR64 genetic background will be useful materials for genetic analysis of agronomic traits.