Abstract: Based on morphological and molecular data, a new species of Gesneriaceae, Primulina yingdeensis, from Guangdong, China, is described and illustrated. It is morphologically most similar to P. hochiensis and P. hochiensis var. rosulata, but the three taxa differ from each other in discrete characters. They share yellow honey guides, but in the new species these are strongly ridged and with glands. At the same time, also based on morphological and molecular data, P. hochiensis var. rosulata is raised to the rank of species, as P. rosulata. Citation: Ning Z.-L., Pan B., Wen F., Kang M. & Zhuang X.-Y. 2016: Primulina yingdeensis, a new species from Guangdong, China, and P. rosulata, a new combination (Gesneriaceae), based on morphological and molecular evidence. — Willdenowia 46: 399–409. doi: http://dx.doi.org/10.3372/wi.46.46308 Version of record first published online on 15 November 2016 ahead of inclusion in December 2016 issue.