Neutral Composition Research Articles

Development of the neutral gas diagnostic system for neutral pressure measurements and gas analysis on the SPARC tokamak.

A suite of plasma diagnostics will be installed on the SPARC tokamak to allow for real-time plasma control, an investigation of high-field tokamak physics, and to de-risk the design of ARC, a compact fusion power plant with the aim to supply electricity to the grid. Among these diagnostics is the neutral gas diagnostics system (NTGS), a set of pressure sensors and gas analyzers used to monitor neutral pressure and gas composition for plasma control, optimization of wall conditioning, and helium ash removal, among other measurement functions linked to operational and scientific research needs. While reliable measurements of neutral pressure and gas composition have been fielded on existing magnetic-confinement fusion devices, SPARC represents a step increase in challenge due to its larger power density, higher field, high vacuum vessel bake temperatures, and higher neutron flux environment, as well as a step decrease in the accessibility for maintenance of in-vessel sensors. Multiple sensor types will be employed to have defense-in-depth and mitigate common failure modes. The NTGS system is currently progressing through final design, working to close out decisions using prototyping and analysis, and then moving on to procuring sensors for assembly and installation on SPARC. This paper outlines the current status of the system design and the diagnostic requirements that motivate neutral gas measurements on SPARC, as well as highlights the planned prototyping activities.

Modeling the plasma composition of 67P/C-G at different heliocentric distances

The Rosetta spacecraft accompanied the comet 67P/C-G for nearly 2 years, collecting valuable data on the neutral and ion composition of the coma. The Rosetta Plasma Consortium (RPC) provided continuous measurements of the in situ plasma density while ROSINA-COPS monitored the neutral composition. In this work, we aim to estimate the composition of the cometary ionosphere at different heliocentric distances of the comet. Läuter et al. (2020) derived the temporal evolution of the volatile sublimation rates for 50 separated time intervals on the orbit of 67P/C-G using the COPS and DFMS data. We use these sublimation rates as inputs in a multifluid chemical-hydrodynamical model for 36 of the time intervals for heliocentric distances <3 au. We compare the total ion densities obtained from our models with the local plasma density measured by the RPC instruments. We find that at the location of the spacecraft, our modeled ion densities match with the in situ measured plasma density within factors of 1−3 for many of the time intervals. We obtain the cometocentric distance variation of the ions H2O+ and H3O+ and the ion groups created respectively by the ionization and protonation of neutral species. We see that H3O+ is dominant at the spacecraft location for nearly all the time intervals while ions created due to protonation are dominant at low cometocentric distances for the intervals near perihelion. We also discuss our ion densities in the context of their detection by DFMS.

A Community Ionosphere‐Thermosphere Observing System Simulation Experiment (OSSE) Tool: Geospace Dynamics Constellation Example

AbstractObserving System Simulation Experiments (OSSEs) provide an effective way to evaluate the impact of assimilating data from a specific observing system on hindcasting, nowcasting, and forecasting of environmental systems. The NSF NCAR's Data Assimilation (DA) Research Testbed/Thermosphere‐Ionosphere‐Electrodynamics General Circulation Model (DART/TIEGCM) tool, to be hosted at the NASA Community Coordinated Modeling Center, serves as a valuable and accessible community resource for quantitatively evaluating the impact of observations from both current and future ionosphere‐thermosphere (IT) observing systems. This study demonstrates the utility of DART/TIEGCM as an IT OSSE tool, using synthetic observations simulated using a currently planned NASA Geospace Dynamics Constellation (GDC) observing system design. Five sets of OSSEs are carried out to compare the effects of assimilating various combinations of prospective GDC observations (e.g., neutral temperature, neutral wind, neutral composition, atomic oxygen ion density, and ion and electron temperature) during a major geomagnetic storm period of the St Patrick's Day Storm on 17 March 2013. The maximum error reduction in neutral temperature and atomic ion oxygen density is 24.6% and 43.3% compared to the control experiment. These OSSEs indicate the benefits of coupled IT DA approaches implemented in DART/TIEGCM to maximize the impact of multi‐parameter IT observations, such as those expected from the GDC mission. Although more work is required to draw any definitive conclusion on the GDC data impact, the study provides an illustrative example of how the DART/TIEGCM community tool can be used to evaluate observational impacts of planned or existing missions for geospace research and applications.

Evaluation of Lipids and Lipid-Related Transcripts in Human and Ovine Theca Cells and an in Vitro Mouse Model Exposed to the Obesogen Chemical Tributyltin.

Exposure to obesogenic chemicals has been reported to result in enhanced adipogenesis, higher adipose tissue accumulation, and reduced ovarian hormonal synthesis and follicular function. We have reported that organotins [tributyltin (TBT) and triphenyltin (TPT)] dysregulate cholesterol trafficking in ovarian theca cells, but, whether organotins also exert lipogenic effects on ovarian cells remains unexplored. We investigated if environmentally relevant exposures to organotins [TBT, TPT, or dibutyltin (DBT)] induce lipid dysregulation in ovarian theca cells and the role of the liver X receptor (LXR) in this effect. We also tested the effect of TBT on oocyte maturation and neutral lipid accumulation, and lipid-related transcript expression in cumulus cells and preimplantation embryos. Primary theca cell cultures derived from human and ovine ovaries were exposed to TBT, TPT, or DBT (1, 10, or ). The effect of these chemical exposures on neutral lipid accumulation, lipid abundance and composition, lipid homeostasis-related gene expression, and cytokine secretion was evaluated using liquid chromatography-mass spectrometry (LC-MS), inhibitor-based methods, cytokine secretion, and lipid ontology analyses. We also exposed murine cumulus-oocyte complexes to TBT and evaluated oocyte maturation, embryo development, and lipid homeostasis-related mRNA expression in cumulus cells and blastocysts. Exposure to TBT resulted in higher intracellular neutral lipids in human and ovine primary theca cells. In ovine theca cells, this effect was dose-dependent, independent of cell stage, and partially mediated by LXR. DBT and TPT resulted in higher intracellular neutral lipids but to a lesser extent in comparison with TBT. More than 140 lipids and 9 cytokines were dysregulated in TBT-exposed human theca cells. Expression of genes involved in lipogenesis and fatty acid synthesis were higher in theca cells, as well as in cumulus cells and blastocysts exposed to TBT. However, TBT did not impact the rates of oocyte maturation or blastocyst development. TBT induced dyslipidemia in primary human and ovine theca cells, which may be responsible for some of the TBT-induced fertility dysregulations reported in rodent models of TBT exposure. https://doi.org/10.1289/EHP13955.

Distribution of carboxy groups in TEMPO-oxidized cellulose nanofibrils prepared from never-dried Japanese cedar holocellulose, Japanese cedar-callus, and bacterial cellulose

We prepared 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-oxidized samples from never-dried Japanese cedar (JC) holocellulose, JC-callus, and bacterial cellulose (BC). The original never-dried samples and their TEMPO-oxidized products were characterized by neutral sugar composition analysis. TEMPO-oxidized cellulose nanofibrils (TEMPO-CNFs) were prepared from the TEMPO-oxidized samples by ultrasonication in water. The carboxy groups in TEMPO-CNFs were position-selectively esterified with 9-anthryl diazomethane (ADAM) to prepare TEMPO-CNF-COOCH2-C14H9 samples, which had UV absorption peak at 365 nm. The mass-average degree of polymerization (DPw) values of 1% lithium chloride/N,N-dimethylacetamide (LiCl/DMAc) solutions of the original samples were determined by size-exclusion chromatography in combination with multi-angle laser-light scattering, ultraviolet absorption, and refractive index detection (SEC/MALLS/UV/RI), and were 5490, 2660, and 2380 for the JC holocellulose, JC-callus, and BC samples, respectively. The TEMPO-CNF-COOCH2-C14H9 sample solutions in 1% LiCl/DMAc were analyzed by SEC/MALLS/UV/RI to obtain SEC elution patterns. The patterns corresponded to the molar mass and carboxy group distributions of the samples, which were detected by RI and UV absorption of anthryl groups, respectively. The carboxy groups existed in the entire molar mass distribution regions of all the TEMPO-CNF samples, although their lower molar mass regions contained higher carboxy group densities. The obtained results indicate that random depolymerization occurred on the cellulose microfibril surfaces at the initial stage of TEMPO-catalyzed oxidation and/or ultrasonication in water. This depolymerization mechanism can explain all the obtained SEC-elution patterns of the TEMPO-CNFs, without considering the presence of periodically disordered regions in the cellulose microfibrils of the never-dried cellulose samples.Graphical abstract

Longitudinal variations of ionospheric responses to the February and April 2023 geomagnetic storms over American and Asian sectors

This paper investigates the ionospheric responses to the February and April 2023 geomagnetic storms using Total Electron Content (TEC) derived from the 10 Global Navigation Satellite System (GNSS) stations at the transition of low to mid-latitudes (Approx. 20 ∼ 30°N) across different longitudes in the American and Asian sectors. Significant variations in both sectors were recorded during the recovery phase and were mainly attributed to the Prompt Penetration Electric Field (PPEF), Disturbance Dynamo Electric Field (DDEF) and the thermospheric neutral composition changes as O/N2 depletion. Similarly, the American and Asian sectors showed TEC enhancements, primarily attributed to the PPEF, during the main phase of the April storm. The negative TEC variations during the recovery phase were observed due to the DDEF and changes in O/N2. In the Asian sector, positive and negative variations were recorded over Pakistan and China, respectively, during the main phase of the April storm. These variations resulted in high and low concentrations of the O/N2 ratio due to Magnetosphere-Ionosphere-Thermosphere coupling.

Numerical Prediction of Changes in Atmospheric Chemical Compositions During a Solar Energetic Particle Event on Mars

AbstractPrecipitation of solar energetic particles (SEPs) into planetary atmospheres causes changes in atmospheric chemical composition through ionization, dissociation, and excitation of atmospheric molecules. In contrast to the terrestrial atmosphere, where depletion of ozone in the polar mesosphere has been studied by observations and models for decades, there have been no studies on the effects of SEPs on the neutral chemical composition of Mars' present‐day atmosphere. This study provided the first estimate of the impacts of SEPs on neutral chemical composition in the present‐day Martian atmosphere coupling a Monte Carlo model and a one‐dimensional photochemical model. Our results showed that ozone density in the Martian atmosphere might decrease in the altitude range of 20–60 km with a factor 10 maximum enhancement occurring at 40 km during a Halloween‐class SEP event due to an enhanced concentration of HOx. The depletion of ozone occurred in the altitude range of 20–60 km, corresponding to the penetration of protons with an energy range of 4.6–46 MeV. Such ozone depletion should be detected by Trace Gas Orbiter/Nadir and Occultation for MArs Discovery during intense SEP event. A 75% depletion of the ozone density at 40 km can be expected during SEP events occurring once in every 1 year. Therefore, ozone concentration in the Martian atmosphere might sufficiently decrease during a SEP event as on Earth, but through different chemical pathways driven by CO2 ionization and CO recombination catalytic cycle.

A New Approach to the Ionosphere at Middle and Low Latitudes under the Geomagnetic Quiet Time of December 2019 by ICON and GOLD Observations

It has been found that the total electron content (TEC) and the ionospheric electric fields indicated by the geomagnetic data showed inconsistent changes with each other at the mid- and low latitudes in both the American and the Asian–Australian sectors during geomagnetic quiet time (GQT) from 30 November to 8 December 2019 (Kpmax = 1.7). Meanwhile, the effects of thermospheric compositions are still indistinct. In this work, we analyze the mid/low-latitude ionospheric variations during this period, utilizing multi-instrument observations. The vertical drift velocities from the Ionospheric Connection Explorer (ICON) show significant variations and are in line with the changes in TEC at low latitudes in both of the two sectors. The zonal electric fields are supposed to play the main role in the TEC changes. This is also confirmed by the ionospheric F2 layer parameters data from the ionosonde stations at Sanya in the Asian–Australian sectors. The correlation between the variations in the geomagnetic H component (ΔH) and ionospheric F-layer electric fields can be affected by solar activity levels. The geomagnetic data ΔH sometimes may not indicate the magnitude of the electric fields in the F-region ionosphere under geomagnetic quiet conditions. The column density ratio of atomic oxygen (O) to molecular nitrogen (N2) (∑O/N2) from the Global Scale Observations of the Limb and Disk (GOLD) showed a strong enhancement at mid-latitudes in the American sector on 30 November. It is speculated that the neutral compositions should make a minor contribution to the changes in TEC during this event, compared with the electric fields.

Impact of chlorine ion chemistry on ozone loss in the middle atmosphere during very large solar proton events

Abstract. Solar coronal mass ejections can accelerate charged particles, mostly protons, to high energies, causing solar proton events (SPEs). Such energetic particles can precipitate upon the Earth's atmosphere, mostly in polar regions because of geomagnetic shielding. Here, SPE-induced chlorine activation due to ion chemistry can occur, and the activated chlorine depletes ozone in the polar middle atmosphere. We use the state-of-the-art 1D stacked-box Exoplanetary Terrestrial Ion Chemistry (ExoTIC) model of atmospheric ion and neutral composition to investigate such events in the Northern Hemisphere (NH). The Halloween SPE that occurred in late October 2003 is used as a test field for our study. This event has been extensively studied before using different 3D models and satellite observations. Our main purpose is to use such a large event that has been recorded by the Michelson Interferometer for Passive Atmospheric Sounding (MIPAS) on the Environmental Satellite (ENVISAT) to evaluate the performance of the ion chemistry model. Sensitivity tests were carried out for different model settings with a focus on the chlorine species of HOCl and ClONO2 as well as O3 and reactive nitrogen, NOy. The model simulations were performed in the Northern Hemisphere at a high latitude of 67.5∘ N, inside the polar cap. Comparison of the simulated effects against MIPAS observations for the Halloween SPE revealed rather good temporal agreement, also in terms of altitude range for HOCl, O3 and NOy. For ClONO2, good agreement was found in terms of altitude range. The model showed ClONO2 enhancements after the peak of the event. The best model setting was the one with full ion chemistry where O(1D) was set to photo-chemical equilibrium. HOCl and ozone changes are very well reproduced by the model, especially for nighttime. HOCl was found to be the main active chlorine species under nighttime conditions, resulting in an increase of more than 0.2 ppbv. Further, ClONO2 enhancements of 0.2–0.3 ppbv have been observed during both daytime and nighttime. Model settings that compared best with MIPAS observations were applied to an extreme solar event that occurred in AD 775, presumably once in a 1000-year event. With the model applied to this scenario, an assessment can be made about what is to be expected at worst for the effects of a SPE on the middle atmosphere, concentrating on the effects of ion chemistry compared to crude parameterizations. Here, a systematic analysis comparing the impact of the Halloween SPE and the extreme event on the Earth's middle atmosphere is presented. As seen from the model simulations, both events were able to perturb the polar stratosphere and mesosphere with a high production of NOy and HOx. Longer-lasting and stronger stratospheric ozone loss was seen for the extreme event. A qualitative difference between the two events and a long-lasting impact on HOCl and HCl for the extreme event were found. Chlorine ion chemistry contributed to stratospheric ozone losses of 2.4 % for daytime and 10 % for nighttime during the Halloween SPE, as seen with time-dependent ionization rates applied to the model. Furthermore, while comparing the Halloween SPE and the extreme scenario, with ionization rate profiles applied just for the event day, the inclusion of chlorine ion chemistry added ozone losses of 10 % and 20 % respectively.

Vertical Variations in Thermospheric O/N2 and the Relationship Between O and N2 Perturbations During a Geomagnetic Storm

AbstractThe ratio of O to N2 number densities (O/N2) at different altitudes is an important parameter in describing thermospheric neutral composition changes and their effects on the ionosphere during geomagnetic storms. However, storm‐induced vertical variations in O/N2 and its dependence on the O and N2 perturbations are still not fully understood. Here, the Thermosphere/Ionosphere Electrodynamics General Circulation Model simulations were used to investigate the responses of thermospheric composition at different pressure levels to the super geomagnetic storm occurred on November 20 and 21 in 2003. Our analysis shows that the behaviors of O/N2 perturbations on different pressure levels are similar above ∼180 km altitude. In the middle and low thermosphere of below ∼300 km, the storm‐time O/N2 decrease is mainly caused by a large reduction of O number density. However, N2 enhancement plays a vital role in O/N2 decreases in the upper thermosphere. The O/N2 enhancement is mainly attributed to the N2 decreases at all pressure levels. The changes of O and N2 number densities at a constant pressure level can be explained by the perturbations of their mass mixing ratio (mmr) and total mass density (ρ). The regions of the O/N2 decrease are characterized by the O mmr decrease and N2 mmr enhancement, whereas the regions of the O/N2 increase are characterized by the O mmr increase and N2 mmr decrease. The ρ value that shows the decrease globally at most pressure levels during the storm either enhance or reduce the O and N2 perturbations.

Space weather impacts on the ionosphere over the southern African mid-latitude region

The ionosphere suffers major perturbations during severe space weather events such as Coronal Mass Ejections (CMEs), solar flares, high-speed streams, and Corotating Interaction Regions (CIRs). The ionosphere can experience depletions or enhancements in Total Electron Content (TEC) during severe space weather conditions. The South African National Space Agency (SANSA) near-real-time (NRT) TEC maps were used to show the ionospheric variability during the geomagnetic storm of 3–8 Nov 2021 over the southern Africa mid-latitude region. The ionosonde TEC, NRT TEC, and the quiet-time AfriTEC model were compared during the 6-day period. A negative ionospheric response was observed during the main and recovery phases of the geomagnetic storm (4–5 Nov 2021). The changes to neutral composition O/N2 was one of the physical processes attributed to the decrease in TEC over the mid-latitude region. The GPS TEC maps showed a very good agreement with ionosonde measurements and the AfriTEC model. A strong east–west TEC gradient was observed occurring between two ionosonde stations.Graphical

N-glycosylation in non-invasive and invasive intraductal papillary mucinous neoplasm

Intraductal papillary mucinous neoplasms (IPMNs), often found incidentally, are potentially malignant cystic tumors of the pancreas. Due to the precancerous nature, IPMNs lacking malignant features should be kept on surveillance. The follow-up relies on magnetic resonance imaging, which has a limited accuracy to define the high-risk patients. New diagnostic methods are thus needed to recognize IPMNs with malignant potential. Here, aberrantly expressed glycans constitute a promising new area of research. We compared the N-glycan profiles of non-invasive IPMN tissues (n = 10) and invasive IPMN tissues (n = 10) to those of non-neoplastic pancreatic controls (n = 5) by matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) mass spectrometry. Both IPMN subgroups showed increased abundance of neutral composition H4N4 and decrease in H3N5F1, increase in sialylation, and decrease in sulfation, as compared to the controls. Furthermore, invasive IPMN showed an increase in terminal N-acetylhexosamine containing structure H4N5, and increase in acidic complex-type glycans, but decrease in their complex fucosylation and sulfation, as compared to the controls. In conclusion, the N-glycan profiles differed between healthy pancreatic tissue and non-invasive and invasive IPMNs. The unique glycans expressed in invasive IPMNs may offer interesting new options for diagnostics.

On the importance of neutral composition and temperature measurements in the 100-200 km altitude region

On the importance of neutral composition and temperature measurements in the 100-200 km altitude region

Whether Sudden Stratospheric Warming Effects Are Seen in the Midlatitude Thermosphere of the Opposite Hemisphere?

AbstractThe reaction of midlatitude daytime foF2 and thermospheric parameters in two Hemispheres has been analyzed for a minor Arctic Sudden Stratospheric Warming (SSW) in January 2008, three major Arctic SSWs in January 2006, 2009, 2013, and a minor Antarctic SSW in September 2019. Arctic SSWs always result in a simultaneous foF2 depression observed at some stations in the Northern Hemisphere but not necessary in the opposite Hemisphere, that is, not all SSWs have global appearance. Thermospheric parameters retrieved from ionospheric observations in two Hemispheres manifest a dependence on the type of SSW (major/minor) and its magnitude. Pronounced thermospheric SSW effects in the opposite (Southern) Hemisphere may be expected only for strong major Arctic SSW events. Retrieved exospheric temperature does not manifest any visible reaction to SSWs both for major and minor SSW events. The duration of foF2 and atomic oxygen decrease related to SSW is 3–5 days in the vicinity of the SSW peak. Both observed neutral gas density and retrieved thermospheric parameters do not manifest a significant difference comparing western and eastern regions of North America during the minor Antarctic SSW in September 2019. Therefore, the previously reported (80–100%) increase in TEC in the western region of North America was not related with variations of neutral composition and winds.

Performance evaluation of IRI, IRI Plas and SAMI2 during the consecutive prolonged solar minimum of cycles 23 and 24 around 100°E

We have examined the performance of the International Reference Ionosphere (IRI 2016), International Reference Ionosphere extended to Plasmasphere (IRI-Plas 2017), and Sami2 is Another Model of the Ionosphere (SAMI2) models for long and deep solar minimum quiet time period around 100°E by comparing the models with the total electron content (TEC) obtained from a chain of Global Navigation Satellite Systems receivers. Results of the study reveal that; all the models overestimate the observed TEC for all stations and seasons, with the highest overestimation shown by IRI Plas. The IRI 2016 model performed better for all stations and seasons than IRI Plas. IRI Plas 2017 model is also simulated by assimilating the TEC derived from GPS (GPS-TEC) and Global Ionospheric Map (GIM-TEC) into the model code. The “no input” option of the model is used as a reference to study the effect of data assimilation on the model's efficacy. It is observed that the assimilation of GPS TEC and GIM-TEC into the model code reproduces TEC quite well for all the quiet days considered during both solar minima. IRI-Plas and IRI 2016 simulation is also compared with SAMI2 simulations for the same space–time configuration. SAMI2 also overestimates the quiet time GNSS TEC during daytime peak hours in all northern and southern hemisphere. Changes are made to the SAMI2 model inputs based on previously reported changes during deep solar minimum. By changing the ionizing EUV, neutral thermospheric composition, and neutral temperature, a good agreement between the simulated and measured data is obtained. However, due to strong tidal effects during low solar activity, additional modifications may be needed to the neutral density, temperature, and EUV. The drift model used in SAMI2 should also be replaced or modified for the low solar activity periods.

Seasonal Transitions in the Thermosphere Inferred from Ionospheric Observations

Ionospheric observations along with CHAMP/STAR neutral gas density measurements were used to retrieve thermospheric parameters and to check whether the equinox transition season exists separately from the December solstice and June solstice seasons. Juliusruh and Boulder ionosonde stations located in “far-from-pole” and “near-pole” longitudinal sectors were analyzed during deep solar minimum in 2008–2009. The results were compared to GOLD column O/N2 ratio observations. The retrieved thermospheric parameters have shown that equinoctial transition period exists separately from the winter one at Juliusruh, while column O/N2 ratios, exospheric temperatures Tex, and vertical plasma drifts related to thermospheric winds retrieved at Boulder for the winter season do not significantly differ from vernal values. This means that the December solstice season just does not exist as it merges with the vernal season in the “near-pole” longitudinal sector. The obtained results indicate that two longitudinal sectors manifest different seasonal variations both in thermospheric circulation and neutral composition.

Changes in chemical structures and molar mass parameters of birch wood powder by ethylene diamine treatment

It is necessary to delignify wood samples and treat them with ethylene diamine (EDA) before they are dissolved in 8% (w/v) lithium chloride/N,N-dimethylacetamide (LiCl/DMAc) prior to size-exclusion chromatography (SEC) analysis. In the present study, the effects of delignifying birch wood powder 0–3 times with NaClO2 and subsequently treating it with EDA on its solubility in 8% (w/v) LiCl/DMAc and its SEC data. The neutral sugar composition of the birch powder was almost unaffected by either delignification or treatment with EDA. Treatment of the birch powder with EDA resulted in 28% solubilities in 8% (w/v) LiCl/DMAc. Approximately 11% of cellulose molecules in the birch wood powder was dissolved, and detected as a high-molar-mass (HMM) fraction in the SEC elution pattern. Each single delignification treatment increased the solubility in 8% (w/v) LiCl/DMAc to 68–74% after EDA treatment. Based on the glucose contents of the delignified samples, almost all cellulose molecules in the delignified samples were dissolved in 8% (w/v) LiCl/DMAc after EDA treatment, and detected as the HMM fractions in the SEC elution patterns. The HMM cellulose molecules in the EDA-treated birch powder had linear random-coil conformations in 1% (w/v) LiCl/DMAc. However, the SEC data suggest that there probably were some chemical linkages between the HMM cellulose molecules and lignin or NaClO2-treated lignin fragments in the HMM fractions.

Changes in Cell Wall Sugar Neutral Composition Contribute to Apple Texture Loss during Storage among Cultivars

Fruit texture affects apples’ quality, consumer preference, and shelf life. The cell wall neutral sugar composition was reported to contribute to apples’ mechanical properties at harvest. However, the contributions of cell wall neutral sugar composition to apple texture loss during storage among different cultivars are still unclear. In this study, six cultivars of the apple fruit were stored at 25 °C for 60 days (i.e., rapid loss of texture: ‘Jiguan’, ‘Yindu’, and ‘Qinguan’; slow loss of texture: ‘Fuji’, ‘Huaguan’, and ‘Cripps pink’). The texture properties, physiological indicators, and expression of cell wall-related genes were investigated every 20 d. The results showed that apple cultivars with rapid texture loss showed a faster loss in flesh hardness and crispness, and a higher level of flesh tightness and pericarp break distance, than the cultivars with slow texture loss. Galactose content showed the closest association with the texture properties among the cell wall neutral sugar compositions. The rapid loss of galacturonic acid content and the expression of MdPG1 were higher in the cultivars with rapid texture loss than in those with slow texture loss. These results indicated that changes in cell wall neutral sugar composition contribute to apple texture loss during storage among cultivars.

Characterization of neutral species in an NH3-Ar plasma generated by a capacitively coupled plasma source at various radio frequencies

Plasma-enhanced atomic layer deposition of SiNx is promising for many device applications, but a lack of growth and material property conformality on three-dimensional device surfaces limits its application. Changing the neutral composition of the nitridation plasma can have an impact on side wall nitridation. In plasma deposition experiments, the role of frequency of the delivered power on neutral species formation is underexplored. We measure the densities of various neutral species generated in a capacitively coupled, 300 mTorr ammonia/argon plasma as a function of driving frequency between 13.56 and 200 MHz using line-of-sight threshold ionization mass spectroscopy. High-density species, which are on the order of 1015 molecules/cm3, such as NH3, H2, H, and N2, as well as lower density species at densities on the order of 1012 molecules/cm3, including N2H2, N2H4, and N2 metastables, were measured and their densities varied with drive frequency.

Neutral Per- and Polyfluoroalkyl Substances in In Situ Landfill Gas by Thermal Desorption–Gas Chromatography–Mass Spectrometry

Landfills receive over half of the municipal solid waste generated in the U.S. Wastes in landfills include consumer products, some of which are known to contain of per- and polyfluoroalkyl substances (PFAS). Although the occurrence of nonvolatile (ionic) PFAS in landfill leachate is established and volatile (neutral) PFAS are found in ambient air near landfills, the neutral PFAS composition of landfill gas (LFG) collected in situ from landfills is unknown. A thermal desorption–gas chromatography–mass spectrometry (TD-GC-MS) sampling and analysis approach was optimized for the quantification of 25 target neutral PFAS, including fluorotelomer alcohols (FTOHs) and five other PFAS classes, in landfill from a gas well or header pipe. Optimized LFG sampling parameters included the use of an explosion-proof pump, a 100 mL/min flow rate through a sorbent tube, and a LFG volume of 350 mL. The method was applied to LFG samples collected from southeastern U.S. landfills for method demonstration. Fluorotelomer alcohols were found at the highest levels, ranging from 830,000–4,900,000 pg/m3, which is approximately 2 orders of magnitude greater than FTOH levels reported in ambient air collected near landfills.