The food system represents a complex, interrelated, and interdependent network of individuals and organizations. Despite the unique characteristics of the food system domain, there has never been a comprehensive analysis of leadership capacity amongst food system professionals. The present study provides an empirical assessment of food system leadership capacity using many leadership instruments. The study included pilot (n = 125) and primary (n = 4185) data collection across 27 food system-related leadership development programs. The results validate the Issue Leadership scale, which comprises seven capacity areas: action, change, communication, critical thinking, strategic planning and visioning, interpersonal traits and characteristics, leadership process, and leadership skills. The Issue Leadership scale captures both perception of importance and current skill level. The study results indicate that food system professionals perceived communication and critical thinking, strategic planning and visioning as the most important leadership capacity areas. Furthermore, respondents indicated the highest level of self-perceived skill in critical thinking, strategic planning and visioning, and interpersonal traits and characteristics. Overall, respondents had higher perceived importance levels (m = 4.17) than skill (m = 3.44), indicating the need for additional leadership capacity development amongst food system professionals. Respondents also indicated similar levels of opinion leadership (m = 3.92), servant leadership (m = 3.82), transformational leadership (m = 3.78), and transactional leadership (m = 3.70), providing an additional empirical assessment of food system professional leadership capacity.