Network Clustering Research Articles

A network approach for multiscale catchment classification using traits

Abstract. The classification of river catchments into groups with similar biophysical characteristics is useful to understand and predict their hydrological behavior. The increasing availability of remote sensing and other large-scale geospatial datasets has enabled the use of advanced data-driven approaches to classify catchments using traits such as topography, geology, climate, land cover, land use, and human influence. Unsupervised clustering algorithms based on the Euclidean distance are commonly used for trait-based classification but are not suitable for highly dimensional data. In this study we present a new network-based method for multi-scale catchment classification, which can be applied to large datasets and used to determine the traits associated with different catchment groups. In this framework, two networks are analyzed in parallel: the first being where the nodes are traits and the second being where the nodes are catchments. In both cases, edges represent pairwise similarity, and a network cluster detection algorithm is used for the classification. The trait network is used to investigate redundancy in the trait data and to condense this information into a small number of interpretable categories. The catchments network is used to classify the catchments into clusters and to identify representative catchments for the different groups using the degree centrality metric. We apply this method to classify 9067 river catchments across the contiguous United States at both regional and continental scales using 274 non-categorical traits. At the continental scale, we identify 25 interpretable trait categories and 34 catchment clusters of sizes greater than 50. We find that catchments with similar trait categories are typically located in the same region, with different spatial patterns emerging among clusters dominated by natural and anthropogenic traits. We also find that the catchment clusters exhibit distinct hydrological behavior based on an analysis of streamflow indices. This network approach provides several advantages over traditional means of classification, including better separation of clusters, the use of alternate similarity metrics that are more suitable for highly dimensional data, and reducing redundancy in the trait information. The paired catchment–trait networks enable analysis of hydrological behavior using the dominant trait categories for each catchment cluster. The approach can be used at multiple spatial scales since the network topologies adjust automatically to reflect the trait patterns at the scale of investigation. Finally, the representative catchments identified as hub nodes in the network can be used to guide transferable observational and modeling strategies. The method is broadly applicable beyond hydrology for classification of other complex systems that utilize different types of trait datasets.

Empowering precise advertising with Fed-GANCC: A novel federated learning approach leveraging Generative Adversarial Networks and group clustering.

In the realm of targeted advertising, the demand for precision is paramount, and the traditional centralized machine learning paradigm fails to address this necessity effectively. Two critical challenges persist in the current advertising ecosystem: the data privacy concerns leading to isolated data islands and the complexity in handling non-Independent and Identically Distributed (non-IID) data and concept drift due to the specificity and diversity in user behavior data. Current federated learning frameworks struggle to overcome these hurdles satisfactorily. This paper introduces Fed-GANCC, an innovative federated learning framework that synergizes Generative Adversarial Networks (GANs) and Group Clustering. The framework incorporates a user data augmentation algorithm predicated on adversarial generative networks to enrich user behavior data, curtail the impact of non-uniform data distribution, and enhance the applicability of the global machine learning model. Unlike traditional approaches, our framework offers user data augmentation algorithms based on adversarial generative networks, which not only enriches user behavior data but also reduces the challenges posed by non-uniform data distribution, thereby enhancing the applicability of the global machine learning (ML) model. The effectiveness of Fed-GANCC is distinctly showcased through experimental results, outperforming contemporary methods like FED-AVG and FED-SGD in terms of accuracy, loss value, and receiver operating characteristic (ROC) indicators within the same computing time. Experimental results vindicate the effectiveness of Fed-GANCC, revealing substantial enhancements in accuracy, loss value, and receiver operating characteristic (ROC) metrics compared to FED-AVG and FED-SGD given the same computational time. These outcomes underline Fed-GANCC's exceptional prowess in mitigating issues such as isolated data islands, non-IID data, and concept drift. With its novel approach to addressing the prevailing challenges in targeted advertising such as isolated data islands, non-IID data, and concept drift, the Fed-GANCC framework stands as a benchmark, paving the way for future advancements in federated learning solutions tailored for the advertising domain. The Fed-GANCC framework promises to offer pivotal insights for the future development of efficient and advanced federated learning solutions for targeted advertising.

Identifying acute illness phenotypes via deep temporal interpolation and clustering network on physiologic signatures

Using clustering analysis for early vital signs, unique patient phenotypes with distinct pathophysiological signatures and clinical outcomes may be revealed and support early clinical decision-making. Phenotyping using early vital signs has proven challenging, as vital signs are typically sampled sporadically. We proposed a novel, deep temporal interpolation and clustering network to simultaneously extract latent representations from irregularly sampled vital signs and derive phenotypes. Four distinct clusters were identified. Phenotype A (18%) had the greatest prevalence of comorbid disease with increased prevalence of prolonged respiratory insufficiency, acute kidney injury, sepsis, and long-term (3-year) mortality. Phenotypes B (33%) and C (31%) had a diffuse pattern of mild organ dysfunction. Phenotype B’s favorable short-term clinical outcomes were tempered by the second highest rate of long-term mortality. Phenotype C had favorable clinical outcomes. Phenotype D (17%) exhibited early and persistent hypotension, high incidence of early surgery, and substantial biomarker incidence of inflammation. Despite early and severe illness, phenotype D had the second lowest long-term mortality. After comparing the sequential organ failure assessment scores, the clustering results did not simply provide a recapitulation of previous acuity assessments. This tool may impact triage decisions and have significant implications for clinical decision-support under time constraints and uncertainty.

Influence of Promotion and Pricing on Purchase Incidence, Demand, and Sales Using Machine Learning

The consumer goods industry is a dynamic and fast-paced sector that faces significant challenges in meeting the consumer’s ever-evolving demands and preferences. Today’s retail businesses focus on optimizing their supply and retail execution to maintain a competitive edge in the market and remain profitable. The most impactful method is to offer promotional events that stimulate large-scale purchases and attract new customers. The patterns of normal sales days, promotion days, and non-promotion days are different and it is vital to capture the influence of promotions on demand and sales. Thus, it is vital to understand the effects of promotion and plan them. This paper aims to understand the influence of promotion and pricing strategies for FMCG retail businesses to maximize demand for each brand. Explore the use of Machine Learning (ML) and Deep Learning models such as Clustering and Neural Networks to identify and understand the various demand patterns to analyse the influence of promotion and pricing on demand, and enable businesses to respond more quickly to changes in the market by enabling them to make better-informed decisions that can mitigate risks associated with the impact of disruptions and to ensure the continuity of the business.

Urbanization and Bottom-up Politics in Palestine: The Case of Yatta

The West Bank (occupied Palestinian Territories) is a fragmented network of urban clusters while the rural area is slowly chipped away by Israeli settlers and military apparatus. Palestinians’ bodies are squeezed between checkpoints, razor wire, apartheid walls, military watchtowers, barracks but also between state bureaucracy, incorporation of labourers and cultural dispossession. The occupation through multiple devices produces dispossession and the national struggle explodes with violent and unforeseen forms against Israeli settlers and soldiers. The present paper is a reflection based on one year of fieldwork in Yatta’s urban cluster and its rural area in the southern West Bank. By analysing Israeli’s occupation law, the present work aims at explaining how the Palestinian urbanization process is forced under colonial rule with interlinked consequences on cultural and material dispossession, and youth liminal identities. Within the frame of this set of socio-spatial fragmented relations, the distrust of political parties is growing, and political forms are re-territorialized in local struggle. The research inquires why radical subjectivity is emerging in the Yatta social-spatial context produced by occupation law. How, despite the devices of Israeli occupation, aiming at drawing docile bodies and geography, the bottom-up political phenomenon emerging from Yatta’s urban context explodes in sudden violence.

Single-cell data revealed exhaustion of characteristic NK cell subpopulations and T cell subpopulations in hepatocellular carcinoma.

The treatment and prognosis of patients with advanced hepatocellular carcinoma (HCC) have been a major medical challenge. Unraveling the landscape of tumor immune infiltrating cells (TIICs) in the immune microenvironment of HCC is of great significance to probe the molecular mechanisms. Based on single-cell data of HCC, the cell landscape was revealed from the perspective of TIICs. Special cell subpopulations were determined by the expression levels of marker genes. Differential expression analysis was conducted. The activity of each subpopulation was determined based on the highly expressed genes. CTLA4+ T-cell subpopulations affecting the prognosis of HCC were determined based on survival analysis. A single-cell regulatory network inference and clustering analysis was also performed to determine the transcription factor regulatory networks in the CTLA4+ T cell subpopulations. 10 cell types were identified and NK cells and T cells showed high abundance in tumor tissues. Two NK cells subpopulations were present, FGFBP2+ NK cells, B3GNT7+ NK cells. Four T cells subpopulations were present, LAG3+ T cells, CTLA4+ T cells, RCAN3+ T cells, and HPGDS+ Th2 cells. FGFBP2+ NK cells, and CTLA4+ T cells were the exhaustive subpopulation. High CTLA4+ T cells contributed to poor prognostic outcomes and promoted tumor progression. Finally, a network of transcription factors regulated by NR3C1, STAT1, and STAT3, which were activated, was present in CTLA4+ T cells. CTLA4+ T cell subsets in HCC exhibited functional exhaustion characteristics that probably inhibited T cell function through a transcription factor network dominated by NR3C1, STAT1, and STAT3.

Research on electric vehicle charging load prediction method based on spectral clustering and deep learning network

With the increasing prominence of environmental and energy issues, electric vehicles (EVs) as representatives of clean energy vehicles have experienced rapid development in recent years, and the charging load has also exhibited statistical characteristics. Accurate prediction of EV charging load is crucial to improve grid load dispatch and intelligent level. However, current research on EV charging load prediction still faces challenges such as data reliability, complexity and variability of charging behavior, uncertainty, and lack of standardization methods. Therefore, this paper proposes an electric vehicle charging load prediction method based on spectral clustering and deep learning network (SC-CNN-LSTM). Firstly, to address the insufficient amount of EV charging load data, this paper proposes to use Monte Carlo simulation to sample and simulate historical load data. Then, in order to identify the internal structure and patterns of charging load, the sampled and simulated dataset is clustered using spectral clustering, dividing the data into different clusters, where each cluster represents samples with similar charging load characteristics. Finally, based on the different sample features of each cluster, corresponding CNN-LSTM models are constructed and trained and predict using the respective data. By modifying the model parameters, the prediction accuracy of the model is improved. Through comparative experiments, the proposed method in this paper has significantly improved prediction accuracy compared to traditional prediction methods without clustering, thus validating the effectiveness and practicality of the method.

Demand Clustering and Spectrum Allocation (DCSA) problem in P2MP network with time-varying traffic

In this paper, the Demand Clustering and Spectrum Allocation (DCSA) problem in a point-to-multipoint (P2MP) network with time-varying traffic is studied. This article introduces several heuristic techniques that can be applied to traffic aggregation for P2MP optical network architectures employing digital subcarrier multiplexing. Based on the transmitter constraint, the algorithms assign individual subcarriers (SCs) to receiving nodes with uncorrelated traffic profiles. The simulation results show that the proposed algorithms outperform the clustering methods.

Network analysis of anxiety and depressive symptoms during the COVID-19 pandemic in older adults in the United Kingdom

The health crisis caused by COVID-19 in the United Kingdom and the confinement measures that were subsequently implemented had unprecedented effects on the mental health of older adults, leading to the emergence and exacerbation of different comorbid symptoms including depression and anxiety. This study examined and compared depression and anxiety symptom networks in two specific quarantine periods (June–July and November–December) in the older adult population in the United Kingdom. We used the database of the English Longitudinal Study of Aging COVID-19 Substudy, consisting of 5797 participants in the first stage (54% women) and 6512 participants in the second stage (56% women), all over 50 years of age. The symptoms with the highest centrality in both times were: “Nervousness (A1)” and “Inability to relax (A4)” in expected influence and predictability, and “depressed mood (D1”; bridging expected influence). The latter measure along with "Irritability (A6)" overlapped in both depression and anxiety clusters in both networks. In addition, a the cross-lagged panel network model was examined in which a more significant influence on the direction of the symptom "Nervousness (A1)" by the depressive symptoms of "Anhedonia (D6)", "Hopelessness (D7)", and "Sleep problems (D3)" was observed; the latter measure has the highest predictive capability of the network. The results report which symptoms had a higher degree of centrality and transdiagnostic overlap in the cross-sectional networks (invariants) and the cross-lagged panel network model of anxious and depressive symptomatology.

Single cell analysis reveals the roles and regulatory mechanisms of type-I interferons in Parkinson’s disease

The pathogenesis of Parkinson’s disease (PD) is strongly associated with neuroinflammation, and type I interferons (IFN-I) play a crucial role in regulating immune and inflammatory responses. However, the specific features of IFN in different cell types and the underlying mechanisms of PD have yet to be fully described. In this study, we analyzed the GSE157783 dataset, which includes 39,024 single-cell RNA sequencing results for five PD patients and six healthy controls from the Gene Expression Omnibus database. After cell type annotation, we intersected differentially expressed genes in each cell subcluster with genes collected in The Interferome database to generate an IFN-I-stimulated gene set (ISGs). Based on this gene set, we used the R package AUCell to score each cell, representing the IFN-I activity. Additionally, we performed monocle trajectory analysis, and single-cell regulatory network inference and clustering (SCENIC) to uncover the underlying mechanisms. In silico gene perturbation and subsequent experiments confirm NFATc2 regulation of type I interferon response and neuroinflammation. Our analysis revealed that microglia, endothelial cells, and pericytes exhibited the highest activity of IFN-I. Furthermore, single-cell trajectory detection demonstrated that microglia in the midbrain of PD patients were in a pro-inflammatory activation state, which was validated in the 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD mouse model as well. We identified transcription factors NFATc2, which was significantly up-regulated and involved in the expression of ISGs and activation of microglia in PD. In the 1-Methyl-4-phenylpyridinium (MPP+)-induced BV2 cell model, the suppression of NFATc2 resulted in a reduction in IFN-β levels, impeding the phosphorylation of STAT1, and attenuating the activation of the NF-κB pathway. Furthermore, the downregulation of NFATc2 mitigated the detrimental effects on SH-SY5Y cells co-cultured in conditioned medium. Our study highlights the critical role of microglia in type I interferon responses in PD. Additionally, we identified transcription factors NFATc2 as key regulators of aberrant type I interferon responses and microglial pro-inflammatory activation in PD. These findings provide new insights into the pathogenesis of PD and may have implications for the development of novel therapeutic strategies.

Simulation of Full HIV Cluster Networks in a Nationally Representative Model Indicates Intervention Opportunities.

Clusters of rapid HIV transmission in the United States are increasingly recognized through analysis of HIV molecular sequence data reported to the National HIV Surveillance System. Understanding the full extent of cluster networks is important to assess intervention opportunities. However, full cluster networks include undiagnosed and other infections that cannot be systematically observed in real life. We replicated HIV molecular cluster networks during 2015-2017 in the United States using a stochastic dynamic network simulation model of sexual transmission of HIV. Clusters were defined at the 0.5% genetic distance threshold. Ongoing priority clusters had growth of ≥3 diagnoses/year in multiple years; new priority clusters first had ≥3 diagnoses/year in 2017. We assessed the full extent, composition, and transmission rates of new and ongoing priority clusters. Full clusters were 3-9 times larger than detected clusters, with median detected cluster sizes in new and ongoing priority clusters of 4 (range 3-9) and 11 (range 3-33), respectively, corresponding to full cluster sizes with a median of 14 (3-74) and 94 (7-318), respectively. A median of 36.3% (range 11.1%-72.6%) of infections in the full new priority clusters were undiagnosed. HIV transmission rates in these clusters were >4 times the overall rate observed in the entire simulation. Priority clusters reflect networks with rapid HIV transmission. The substantially larger full extent of these clusters, high proportion of undiagnosed infections, and high transmission rates indicate opportunities for public health intervention and impact.

Regulation of phonon localization on thermal transport in complex networks.

The regulation of thermal transport is a challenging topic in complex networks. At present, the hidden physical mechanism behind thermal transport is poorly understood. This paper addresses this issue by proposing a complex network model that focuses on the thermal transport regulation through the manipulation of the network's degree distribution and clustering coefficient. Our findings indicate that increasing the degree distribution regulation parameter σ leads to reduced phonon localization and improved thermal transport efficiency. Conversely, increasing the clustering coefficient c results in enhanced phonon localization and reduced thermal transport efficiency. Meanwhile, by calculating the pseudodispersion relation of the network, we find that the maximum (or the second smallest) eigenfrequency decreases with increasing σ (or c). Finally, we elucidate that phonon localization plays a pivotal role in the thermal transport of the network, as demonstrated through density of states and the participation ratio.

문학 글쓰기의 과제 수행에 대한 분석 연구

Objectives The purpose of this study is to examine what students at G and K universities recognize and make meaning of after appreciating literary works, and to show the performance results.
 Methods For this purpose, this study used students' writing assignments conducted at two universities as analysis data. As a method of statistically analyzing text data, KH-coder 3 (Folder), a corpus research method, was used to conduct K University assignments such as ‘extracted word correspondence analysis, extracted word multidimensional scaling method, hierarchical cluster analysis, network analysis, and self-organizing instruction method.’ was used to present statistical results intuitively, focusing on typical cases by type. As for the analysis method of G University's project, we presented the results of the task analysis through three methods: ‘correspondence analysis, network analysis, and hierarchical cluster analysis’, and presented some conclusions focusing on typical cases by type.
 Results First, through texts, students at K University remember their somewhat turbulent times and learn about where their foundation came from, how they lived in relationships with their family and friends. It showed that they had come. It had the effect of soothing the wounded heart of the present through the experience of writing down past memories and memories. Second, as a result of student responses at G University, there was a tendency to interpret the story as a story about an immature boy, Namu, experiencing loss, accepting the loss and growing, and also interpreting loss as the basis for growth. Additionally, students responded that they understood the two characters as complementary and aimed for a relationship of growth. Third, while watching the character's growth process, students projected their inner self and showed a journey to self-understanding. And, he said that he applied the characters' motifs of innocence, love, and memories to his own life and felt comfort. In addition, positive writing responses were shown regarding the importance of nature and life.
 Conclusions In this study, we applied literary writing teaching and learning activities that can promote self-growth and found the results to be meaningful in literary education.

Transcriptomics- and Genomics-Guided Drug Repurposing for the Treatment of Vesicular Hand Eczema.

Vesicular hand eczema (VHE), a clinical subtype of hand eczema (HE), showed limited responsiveness to alitretinoin, the only approved systemic treatment for severe chronic HE. This emphasizes the need for alternative treatment approaches. Therefore, our study aimed to identify drug repurposing opportunities for VHE using transcriptomics and genomics data. We constructed a gene network by combining 52 differentially expressed genes (DEGs) from a VHE transcriptomics study with 3 quantitative trait locus (QTL) genes associated with HE. Through network analysis, clustering, and functional enrichment analyses, we investigated the underlying biological mechanisms of this network. Next, we leveraged drug-gene interactions and retrieved pharmaco-transcriptomics data from the DrugBank database to identify drug repurposing opportunities for (V)HE. We developed a drug ranking system, primarily based on efficacy, safety, and practical and pricing factors, to select the most promising drug repurposing candidates. Our results revealed that the (V)HE network comprised 78 genes that yielded several biological pathways underlying the disease. The drug-gene interaction search together with pharmaco-transcriptomics lookups revealed 123 unique drug repurposing opportunities. Based on our drug ranking system, our study identified the most promising drug repurposing opportunities (e.g., vitamin D analogues, retinoids, and immunomodulating drugs) that might be effective in treating (V)HE.

Enhancing Traffic Routing Inside a Network through IoT Technology & Network Clustering by Selecting Smart Leader Nodes

. IoT networking uses real items as stationary or mobile nodes. Mobile nodes complicate networking. Internet of Things (IoT) networks have a lot of control overhead messages because devices are mobile. These signals are generated by the constant flow of control data as such device identity, geographical positioning, node mobility, device configuration, and others. Network clustering is a popular overhead communication management method. Many cluster-based routing methods have been developed to address system restrictions. Node clustering based on the Internet of Things (IoT) protocol, may be used to cluster all network nodes according to predefined criteria. Each cluster will have a Smart Designated Node. SDN cluster management is efficient. Many intelligent nodes remain in the network. The network design spreads these signals. This paper presents an intelligent and responsive routing approach for clustered nodes in IoT networks. An existing method builds a new sub-area clustered topology. The Nodes Clustering Based on the Internet of Things (NCIoT) method improves message transmission between any two nodes. This will facilitate the secure and reliable interchange of healthcare data between professionals and patients. NCIoT is a system that organizes nodes in the Internet of Things (IoT) by grouping them together based on their proximity. It also picks SDN routes for these nodes. This approach involves selecting one option from a range of choices and preparing for likely outcomes problem addressing limitations on activities is a primary focus during the review process. Predictive inquiry employs the process of analyzing data to forecast and anticipate future events. This document provides an explanation of compact units. The Predictive Inquiry Small Packets (PISP) improved its backup system and partnered with SDN to establish a routing information table for each intelligent node, resulting in higher routing performance. Both principal and secondary roads are available for use. The simulation findings indicate that NCIoT algorithms outperform CBR protocols. Enhancements lead to a substantial 78% boost in network performance. In addition, the end-to-end latency dropped by 12.5%. The PISP methodology produces 5.9% more inquiry packets compared to alternative approaches. The algorithms are constructed and evaluated against academic ones.

Single-Cell Nuclear Sequencing Atlas Revealed the Induction of Parkinson's Disease by RELN+ Neuron 3 and the Gene Regulatory Network of MSRA.

To determine the cell types that promoted the progression of Parkinson's disease (PD) using the substantia nigra in the brain tissues derived from patients with PD and normal controls. PD is an incurable neurodegenerative disease that threatens the physical activity of the aging population, and the complex molecular mechanisms remain be comprehensively elucidated. To describe potential disease-promoting cell types in PD and to provide a theoretical basis. Single-cell nuclear sequencing data of nine PD samples and control samples from Gene Expression Omnibus (GEO) were included, and heterogeneous cell subpopulations in the substantia nigra were identified by annotation analysis. Potential pathogenic cell subpopulations of PD were determined based on the expression data of marker genes. Cell differentiation trajectories and communication networks were generated by Pseudotime trajectory analysis and cell communication analysis. Furthermore, single-- cell regulatory network inference and clustering (SCENIC) analysis was conducted to determine the regulatory network of transcription factor-target genes in PD. Among the nine cell subpopulations classified, RELN+neuron 3 showed reduced abundance and dopamine secretion capacity in PD and was therefore considered as a promoter of PD pathogenesis and progression. The regulatory network of MSRA action was involved in the developmental process of cells in the central nervous system, indicating that MSRA and its targets might serve as potential therapeutic targets for PD. RELN+neuron 3 had two directions of differentiation, specifically, branch 1 exhibited a high apoptotic profile and branch 2 exhibited a high cell death profile. In addition, the intensity of EPHA and EPHB signaling was attenuated between RELN+neuron 3 and other cell subpopulations. To conclude, this study identified a subpopulation of RELN+neuron 3 cells with markedly reduced abundance in the brain substantia nigra in PD. The MSRA-involved gene regulatory networks was considered as a novel therapeutic network for PD.

Implementation of the Provisions of the Cluster Theory into the Accounting System: Genesis and Evolution

In current economic conditions, a large number of enterprises carry out their activities by joining into network (cluster) structures, which is one of the means of improving competitive relations in the external environment. To ensure the integration of the interests of participants in network structures by increasing the coordination and controllability of interactions, a significant place is given to the accounting system as the main source of information. The article aims to study the theoretical and methodological foundations of implementing the cluster economy concept in the accounting system. The methodological basis is the fundamental provisions of modern economic institutional theory and scientific works of scientists. As a result of the research, it was possible to analyze the historical aspects of the development of accounting support for managing cluster structures. The areas of development of accounting based on the provisions of economic theory and taking into account the specific features of cluster structures are identified and substantiated.

Indoor positioning fingerprint database construction based on CSA-DBSCAN and RCVAE-GAN

With the increasing size of buildings, in order to achieve high-precision indoor positioning services, it is a challenging task to build an offline fingerprint database with high quality, high density and less manpower and material consumption. Aiming to solve the problem of low-quality WiFi indoor positioning fingerprint inventory constructed by traditional methods, which affects positioning accuracy and incurs high costs, this paper proposes a method for indoor positioning fingerprint database construction based on Crow Search Algorithm Optimizes Density Clustering (CSA-DBSCAN) and Regressor Conditional VAE Generative Adversarial Network (RCVAE-GAN). Collecting only a tiny amount of sparse reference point position coordinates and RSS data makes it possible to construct a high-quality WiFi indoor positioning fingerprint database. Firstly, the method utilizes the density clustering method based on Crow Search Algorithm Optimization (CSA-DBSCAN) to process RSS data collected from the reference point. This helps minimize the impact of abnormal RSS data on creating the fingerprint database. Secondly, the RCVAE-GAN depth generation model was developed. The model consists of an encoder E, a generator G, a discriminator D, and a regressor R. After constructing the model, the data with abnormal RSS will be removed and input into the model for pre-training and joint training, resulting in a high-quality deep-generation model. Finally, a high-quality and high-density fingerprint database is constructed by combining the collected reference points with fingerprint data generated by the depth generation model. Experimental results show that the proposed method reduces the root mean square error (RMSE) deviation of the generated fingerprint data by 38% and 12% respectively, compared to the RBF interpolation method and the CVAE-GAN method in the same experimental scenario. The constructed fingerprint database is used for positioning, improving positioning accuracy by 70% and 65% respectively. The method described in this paper can construct a high-quality fingerprint database, effectively improving the efficiency of fingerprint database construction and reducing the costs associated with labor and time.

SpiDe-Sr: blind super-resolution network for precise cell segmentation and clustering in spatial proteomics imaging

Spatial proteomics elucidates cellular biochemical changes with unprecedented topological level. Imaging mass cytometry (IMC) is a high-dimensional single-cell resolution platform for targeted spatial proteomics. However, the precision of subsequent clinical analysis is constrained by imaging noise and resolution. Here, we propose SpiDe-Sr, a super-resolution network embedded with a denoising module for IMC spatial resolution enhancement. SpiDe-Sr effectively resists noise and improves resolution by 4 times. We demonstrate SpiDe-Sr respectively with cells, mouse and human tissues, resulting 18.95%/27.27%/21.16% increase in peak signal-to-noise ratio and 15.95%/31.63%/15.52% increase in cell extraction accuracy. We further apply SpiDe-Sr to study the tumor microenvironment of a 20-patient clinical breast cancer cohort with 269,556 single cells, and discover the invasion of Gram-negative bacteria is positively correlated with carcinogenesis markers and negatively correlated with immunological markers. Additionally, SpiDe-Sr is also compatible with fluorescence microscopy imaging, suggesting SpiDe-Sr an alternative tool for microscopy image super-resolution.

Research on the mechanism and policies of polycentric agglomeration for promoting the upgrading of China's position in the global production value chain

Recently, with the increasing trend of anti-globalization, China's manufacturing industry has encountered instability in the supply of upstream products in international trade, a challenge that has given rise to an urgent demand for ways to improve China's position in the global production chain. In response to this challenge, the 14th Five-Year Plan puts forward the strategic goal of building multi-level, multi-node, networked city clusters and trade powerhouses, which provides new development opportunities for China to strengthen its position in the global production chain through the model of polycentric agglomeration. In order to gain a deeper understanding of the impact of polycentric agglomeration on the position of enterprises in the global production chain, this project will use the instrumental variable estimation method to conduct an empirical study. This method can help us accurately identify the causal relationship between polycentric agglomeration and global production chain position and assess the differential impact of different agglomeration patterns on firms' production chain position. Through this study, we expect to reveal how polycentric agglomeration can enhance firms' global production chain positions through mechanisms such as optimizing resource allocation, promoting technological innovation, and strengthening industrial chain synergies. In addition, we plan to explore the impact of polycentric agglomeration on the evolution of firms' roles in global value chains and on international trade competitiveness. The objective of this study is to provide targeted recommendations for policymakers and enterprises to help them achieve high-quality development of China's manufacturing industry by optimizing the structure of polycentric agglomeration in the context of global production chain restructuring. Through this comprehensive study, we expect to provide scientific decision support and action guidelines for China's sustainable upgrading and competitiveness enhancement in global production chains.